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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL256
Library AF
(Link to library)
Clone ID AFL256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFL256P
(Link to Original site)
Representative DNA sequence 
>AFL256 (AFL256Q) /CSM/AF/AFL2-C/AFL256Q.Seq.d/
TATCTTTTTTGTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATT
TTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGAT
AACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGT
AAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACT
AGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGT
AAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTCCA
TACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATAT
TCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAAC
TACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTA
ACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAXX
XXXXXXXXGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCA
AACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCG
GTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGA
ATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTG
AATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAA
TTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTA
TCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCG
ACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCG
AAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACG
AAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAG
AAGAAGTCGCTAAGAAGATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ylfc**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSG
KLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGP
YIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVETDQL
THQYTLVISPDNTYKVLVD---

---DDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDW
NEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGA
IGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKAD
EKLAAEKAAEKEAEEADEEEEEVAKKI
Translated Amino Acid sequence (All Frames) 
Frame A:
ylfc**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSG
KLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGP
YIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVETDQL
THQYTLVISPDNTYKVLVD---

---DDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDW
NEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGA
IGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKAD
EKLAAEKAAEKEAEEADEEEEEVAKKI

Frame B:
iffvnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkmvnqv
n*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkklivvvh
tssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklkpin*
lintlwsfhqitptkyssi---

---mignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrig
mkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmnstslmisvl
svsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppklkrerklt
kn*lpkkllkkkpkklmkkkkkslrr

Frame C:
sfllikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rrw*ir*
issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*lwwsi
hqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*nrsin
ssihfghftr*hlqstrr---

---*lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle
*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcy
rfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*rekes*r
kiscrksc*krsrrs**rrrrsr*ed
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL256 (AFL256Q) /CSM/AF/AFL2-C/AFL256Q.Seq.d/                       1994   0.0
VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                       1015   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                       1015   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                       1015   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                       1015   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                       1015   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1015   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                       1015   0.0
SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                       1015   0.0
SFJ301 (SFJ301Q) /CSM/SF/SFJ3-A/SFJ301Q.Seq.d/                       1015   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1015   0.0    3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    84   1e-25  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   2e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-10  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   4e-10  2
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   4e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   4e-09  3
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   6e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   6e-09  1
dna update 2004. 8.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   355   0.0
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   224   e-106
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   222   e-106
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   217   e-106
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   218   e-106
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   223   e-106
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   221   e-106
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   221   e-105
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   221   e-105
L27348_1(L27348|pid:none) Hordeum vulgare calreticulin (CRH1) mR...   220   e-105
protein update 2009. 4.25
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for AFL256 is exc
5' end seq. ID AFL256F
5' end seq. 
>AFL256F.Seq
TATCTTTTTTGTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATT
TTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGAT
AACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGT
AAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACT
AGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGT
AAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTCCA
TACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATAT
TCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAAC
TACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTA
ACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATA--
--------
Length of 5' end seq. 598
3' end seq. ID AFL256Z
3' end seq. 
>AFL256Z.Seq
----------GATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATC
CAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGC
CGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTG
GAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGG
TGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATT
AATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGC
TATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCAC
CGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGC
CGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGA
CGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGA
AGAAGAAGTCGCTAAGAAGATT
Length of 3' end seq. 612
Connected seq. ID AFL256P
Connected seq. 
>AFL256P.Seq
TATCTTTTTTGTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATT
TTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGAT
AACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGT
AAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACT
AGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGT
AAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTCCA
TACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATAT
TCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAAC
TACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTA
ACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATA--
--------GATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCA
AACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCG
GTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGA
ATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTG
AATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAA
TTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTA
TCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCG
ACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCG
AAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACG
AAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAG
AAGAAGTCGCTAAGAAGATT
Length of connected seq. 1210
Full length Seq ID -
Full length Seq. -
Length of full length seq. -