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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL249
Library AF
(Link to library)
Clone ID AFL249
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15323-1
Original site URL
Representative seq. ID AFL249P
(Link to Original site)
Representative DNA sequence 
>AFL249 (AFL249Q) /CSM/AF/AFL2-C/AFL249Q.Seq.d/
CTTAATATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTTAAAATGAATTCT
AAATTTGCTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTNTT
TTAGCAGCAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAA
AACAATGAAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTNCTGGTAATGGNGTT
AACGAATTTATTGGNGGTATTATTTTATATGAAGAAACTNTTTACCAAAAGATGGCAGAT
GGTAGATTATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGAT
AAGGGNGTTGTNCCAATTCCAGGTNCCGATGGTGAAACCTCAACCCAAGGTTTANATGGT
CTTGCAGAGCGTTGTAXXXXXXXXXXTCCCAATTATCAATCACTGAAAATGCATACACAT
TAGCTCGTTATGCTGCAATTTGTCAAGAGAATGGTTTGGTCCCAATCGTCGAACCAGAAA
TCTTAATGGATGGTAATCACACTGTCGAGCAATCTGCTGAAGTCACTGAGAAAGTTTTAG
CTGCAGTTTTCAAAGCACTCAATGACCATCATATCTTATTAGAAGGTGCCTTATTAAAAC
CAAATATGGTCGTCAATGGTACCGACTGTCCAATCAAAGCCACCTCTGAACAAATTGGTA
AATTCACCGTCCGTACCCTTCAACGTACTGTTCCACCAGCTCTTACTGGTGTTGTTTTCT
TATCTGGTGGTCAAACTGAAATTGAAGCCACTGCAAACTTAAACGCTATGAATGTCTTAC
CAAATCGTCCATGGGCTCTCTCTTTCTCATACGGTCGTGCCTTACAGGCCTCTGTCATCT
CCACTTGGAAAGGTGAATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTG
CCAAGTGTAACTCTCTTGCTCAACTCGGTAAATACACTGGTGAGGAATCAACTGGTTCAG
CATCTAATCATTATATGTAAAAGATTACAAATATTAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LNILYCTPLYYILFFFKMNSKFADELIATAKAIVAPGKGXLAADESTNTIGARFKKINLE
NNEENRRAYRELLIGXGNGVNEFIGGIILYEETXYQKMADGRLFTDVLREQKIHIGIKVD
KGVVPIPGXDGETSTQGLXGLAERC---

---SQLSITENAYTLARYAAICQENGLVPIVEPEILMDGNHTVEQSAEVTEKVLAAVFKA
LNDHHILLEGALLKPNMVVNGTDCPIKATSEQIGKFTVRTLQRTVPPALTGVVFLSGGQT
EIEATANLNAMNVLPNRPWALSFSYGRALQASVISTWKGESANVEAARKVYLHRAKCNSL
AQLGKYTGEESTGSASNHYM*kitnik
Translated Amino Acid sequence (All Frames) 
Frame A:
LNILYCTPLYYILFFFKMNSKFADELIATAKAIVAPGKGXLAADESTNTIGARFKKINLE
NNEENRRAYRELLIGXGNGVNEFIGGIILYEETXYQKMADGRLFTDVLREQKIHIGIKVD
KGVVPIPGXDGETSTQGLXGLAERC---

---SQLSITENAYTLARYAAICQENGLVPIVEPEILMDGNHTVEQSAEVTEKVLAAVFKA
LNDHHILLEGALLKPNMVVNGTDCPIKATSEQIGKFTVRTLQRTVPPALTGVVFLSGGQT
EIEATANLNAMNVLPNRPWALSFSYGRALQASVISTWKGESANVEAARKVYLHRAKCNSL
AQLGKYTGEESTGSASNHYM*kitnik

Frame B:
lifyiahhytiyffflk*ilnllmn*lqllkql*hqvkvf*qqmnqpiqlelvskrli*k
tmkrteeptenf*lvlvmxltnllxvlfymkklftkrwqmvdysqmf*enkrftlvlkli
rxlxqfqvpmvkpqpkvxmvlqsvv---

---pnyqslkmhth*lvmlqfvkrmvwsqssnqks*wmvitlssnllkslrkf*lqfskh
smtiisy*kvpy*nqiwssmvptvqskpplnklvnspsvpfnvlfhqlllvlfsylvvkl
klkplqt*tl*msyqivhglslshtvvpyrplssplgkvnlptlkllvkfiyivpsvtll
lnsvntlvrnqlvqhliiickrlqil

Frame C:
*yfilhtiilytfff*nef*ic**incnc*sncstr*rxfssr*inqynwssfqkd*frk
q*reqkslqrtfnwxw*wx*riywxyyfi*rnxlpkdgrw*iihrcfkrtkdshwy*s**
gxcxnsrxrw*nlnprfxwscral---

---piinh*kcihisslccnlsrewfgpnrrtrnlngw*shcraic*sh*esfscsfqst
q*psylirrcliktkygrqwyrlsnqshl*tnw*ihrpypstycstssywccfliwwsn*
n*shcklkryecltkssmgslflirscltglchlhler*icqr*scs*slftscqv*lsc
str*ihw*ginwfsi*slyvkdyky*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL249 (AFL249Q) /CSM/AF/AFL2-C/AFL249Q.Seq.d/                       1919   0.0
SFD187 (SFD187Q) /CSM/SF/SFD1-D/SFD187Q.Seq.d/                       1197   0.0
AFN510 (AFN510Q) /CSM/AF/AFN5-A/AFN510Q.Seq.d/                       1191   0.0
VFJ101 (VFJ101Q) /CSM/VF/VFJ1-A/VFJ101Q.Seq.d/                       1183   0.0
VFI422 (VFI422Q) /CSM/VF/VFI4-A/VFI422Q.Seq.d/                       1183   0.0
VFD501 (VFD501Q) /CSM/VF/VFD5-A/VFD501Q.Seq.d/                       1183   0.0
VFB543 (VFB543Q) /CSM/VF/VFB5-B/VFB543Q.Seq.d/                       1183   0.0
SFI819 (SFI819Q) /CSM/SF/SFI8-A/SFI819Q.Seq.d/                       1183   0.0
SFI292 (SFI292Q) /CSM/SF/SFI2-D/SFI292Q.Seq.d/                       1183   0.0
SFG307 (SFG307Q) /CSM/SF/SFG3-A/SFG307Q.Seq.d/                       1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1132   0.0    4
BM167314|BM167314.1 EST569837 PyBS Plasmodium yoelii yoelii cDNA clone PYCOH24 5' end, mRNA sequence.    62   2e-13  3
AF247059|AF247059.1 Plasmodium yoelii YM fructose 1,6-bisphosphate aldolase gene, partial cds.    62   3e-13  3
AF247061|AF247061.1 Plasmodium vinckei fructose 1,6-bisphosphate aldolase gene, partial cds.    54   3e-13  3
AF247060|AF247060.1 Plasmodium chabaudi AS fructose 1,6-bisphosphate aldolase gene, partial cds.    54   3e-13  3
AF247062|AF247062.1 Plasmodium berghei QIMR fructose 1,6-bisphosphate aldolase gene, partial cds.    62   2e-11  3
AJ768835|AJ768835.1 Populus euphratica EST, clone P0000100022C12F1.    56   6e-09  2
AJ767555|AJ767555.1 Populus euphratica EST, clone P0000100007E09F1.    56   7e-09  2
M81793|M81793.1 Plasmodium berghei fructosebisphosphate aldolase (ALDO-2) gene, complete cds.    54   1e-08  3
CD479311|CD479311.1 eca01-7ms1-b11 Eca01 Eschscholzia californica cDNA clone eca01-7ms1-b11 5', mRNA sequence.    72   2e-08  1
dna update 2004. 8.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86A67) RecName: Full=Fructose-bisphosphate aldolase;          ...   379   e-159
EF144942_1(EF144942|pid:none) Populus trichocarpa clone WS0111_B...   243   4e-89
AL162506_11(AL162506|pid:none) Arabidopsis thaliana DNA chromoso...   235   4e-89
EF146691_1(EF146691|pid:none) Populus trichocarpa clone WS01212_...   238   5e-89
AF308587_1(AF308587|pid:none) Fragaria x ananassa cytosolic aldo...   235   8e-89
AY062566_1(AY062566|pid:none) Arabidopsis thaliana fructose-bisp...   234   1e-88
AF247060_1(AF247060|pid:none) Plasmodium chabaudi AS fructose 1,...   244   1e-88
AM910994_614(AM910994|pid:none) Plasmodium knowlesi strain H chr...   248   2e-88
AF247063_1(AF247063|pid:none) Plasmodium vivax WDK fructose 1,6-...   246   6e-88
DQ874472_1(DQ874472|pid:none) Plasmodium vivax strain A11 fructo...   246   6e-88
protein update 2009. 4.25
PSORT 

 psg: 0.95  gvh: 0.37  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFL249 is cyt
5' end seq. ID AFL249F
5' end seq. 
>AFL249F.Seq
CTTAATATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTTAAAATGAATTCT
AAATTTGCTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTNTT
TTAGCAGCAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAA
AACAATGAAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTNCTGGTAATGGNGTT
AACGAATTTATTGGNGGTATTATTTTATATGAAGAAACTNTTTACCAAAAGATGGCAGAT
GGTAGATTATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGAT
AAGGGNGTTGTNCCAATTCCAGGTNCCGATGGTGAAACCTCAACCCAAGGTTTANATGGT
CTTGCAGAGCGTTGTA----------
Length of 5' end seq. 436
3' end seq. ID AFL249Z
3' end seq. 
>AFL249Z.Seq
----------TCCCAATTATCAATCACTGAAAATGCATACACATTAGCTCGTTATGCTGC
AATTTGTCAAGAGAATGGTTTGGTCCCAATCGTCGAACCAGAAATCTTAATGGATGGTAA
TCACACTGTCGAGCAATCTGCTGAAGTCACTGAGAAAGTTTTAGCTGCAGTTTTCAAAGC
ACTCAATGACCATCATATCTTATTAGAAGGTGCCTTATTAAAACCAAATATGGTCGTCAA
TGGTACCGACTGTCCAATCAAAGCCACCTCTGAACAAATTGGTAAATTCACCGTCCGTAC
CCTTCAACGTACTGTTCCACCAGCTCTTACTGGTGTTGTTTTCTTATCTGGTGGTCAAAC
TGAAATTGAAGCCACTGCAAACTTAAACGCTATGAATGTCTTACCAAATCGTCCATGGGC
TCTCTCTTTCTCATACGGTCGTGCCTTACAGGCCTCTGTCATCTCCACTTGGAAAGGTGA
ATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTGCCAAGTGTAACTCTCT
TGCTCAACTCGGTAAATACACTGGTGAGGAATCAACTGGTTCAGCATCTAATCATTATAT
GTAAAAGATTACAAATATTAAA
Length of 3' end seq. 612
Connected seq. ID AFL249P
Connected seq. 
>AFL249P.Seq
CTTAATATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTTAAAATGAATTCT
AAATTTGCTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTNTT
TTAGCAGCAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAA
AACAATGAAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTNCTGGTAATGGNGTT
AACGAATTTATTGGNGGTATTATTTTATATGAAGAAACTNTTTACCAAAAGATGGCAGAT
GGTAGATTATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGAT
AAGGGNGTTGTNCCAATTCCAGGTNCCGATGGTGAAACCTCAACCCAAGGTTTANATGGT
CTTGCAGAGCGTTGTA----------TCCCAATTATCAATCACTGAAAATGCATACACAT
TAGCTCGTTATGCTGCAATTTGTCAAGAGAATGGTTTGGTCCCAATCGTCGAACCAGAAA
TCTTAATGGATGGTAATCACACTGTCGAGCAATCTGCTGAAGTCACTGAGAAAGTTTTAG
CTGCAGTTTTCAAAGCACTCAATGACCATCATATCTTATTAGAAGGTGCCTTATTAAAAC
CAAATATGGTCGTCAATGGTACCGACTGTCCAATCAAAGCCACCTCTGAACAAATTGGTA
AATTCACCGTCCGTACCCTTCAACGTACTGTTCCACCAGCTCTTACTGGTGTTGTTTTCT
TATCTGGTGGTCAAACTGAAATTGAAGCCACTGCAAACTTAAACGCTATGAATGTCTTAC
CAAATCGTCCATGGGCTCTCTCTTTCTCATACGGTCGTGCCTTACAGGCCTCTGTCATCT
CCACTTGGAAAGGTGAATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTG
CCAAGTGTAACTCTCTTGCTCAACTCGGTAAATACACTGGTGAGGAATCAACTGGTTCAG
CATCTAATCATTATATGTAAAAGATTACAAATATTAAA
Length of connected seq. 1048
Full length Seq ID -
Full length Seq. -
Length of full length seq. -