>AFL230 (AFL230Q) /CSM/AF/AFL2-B/AFL230Q.Seq.d/
XXXXXXXXXXCATCANCAACAACANCAACAACAATTACAACAACAACAACAACAACAACA
ACAACAACAACAACAACAACAACAAATGTATCAACAACAAAGACAAAACAATAACCATCA
ACAACAAACTAATCAACCCCAACAAATGCAACAGCAACAACAAGAACAACAACAATACAC
TGAAGAACAATACGAACAACCACAAGAAGAACAACAATACCAAGAAGAACAACAACAATA
CCAAGAA

---IXNNXNNNYNNNNNNNNNNNNNNNKCINNKDKTITINNKLINPNKCNSNNKNNNNTL
KNNTNNHKKNNNTKKNNNNTK

Frame A:
---IXNNXNNNYNNNNNNNNNNNNNNNKCINNKDKTITINNKLINPNKCNSNNKNNNNTL
KNNTNNHKKNNNTKKNNNNTK


Frame B:
---sxttxttittttttttttttttttnvsttktkq*psttn*stptnatattrtttih*
rtirtttrrttiprrtttipr


Frame C:
---xqqxqqqlqqqqqqqqqqqqqqqqmyqqqrqnnnhqqqtnqpqqmqqqqqeqqqyte
eqyeqpqeeqqyqeeqqqyqe

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Gapped
Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 0
Number of Sequences: 97611
Number of extensions: 0
Number of successful extensions: 0
Number of sequences better than 10.0: 0
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 0
length of query: 247
length of database: 80,480,566
effective HSP length: 17
effective length of query: 230
effective length of database: 78,821,179
effective search space: 18128871170
effective search space used: 18128871170
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 0
Number of Sequences: 37578590
Number of extensions: 0
Number of successful extensions: 0
Number of sequences better than 10.0: 0
length of query: 247
length of database: Z,316,359,891
effective HSP length: 22
effective length of query: 225
effective length of database: Y,489,630,911
effective search space: 9335166954975
effective search space used: 9335166954975
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)
[blastall] WARNING:  [000.000]  AFL230: Blast: No valid letters to be indexed on context 0

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

 ***** No hits found ******

Lambda     K      H
   0.200   0.0445   0.0655

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 2,612,217

 psg: 0.54  gvh: 0.22  alm: 0.69  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 24.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFL230 is nuc

>AFL230Z.Seq
----------CATCANCAACAACANCAACAACAATTACAACAACAACAACAACAACAACA
ACAACAACAACAACAACAACAACAAATGTATCAACAACAAAGACAAAACAATAACCATCA
ACAACAAACTAATCAACCCCAACAAATGCAACAGCAACAACAAGAACAACAACAATACAC
TGAAGAACAATACGAACAACCACAAGAAGAACAACAATACCAAGAAGAACAACAACAATA
CCAAGAA