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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL229
Library AF
(Link to library)
Clone ID AFL229
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15511-1
Original site URL
Representative seq. ID AFL229P
(Link to Original site)
Representative DNA sequence 
>AFL229 (AFL229Q) /CSM/AF/AFL2-B/AFL229Q.Seq.d/
AATTTATAAATAAAATATATGCATATATTCAAATAGTTTTATAAATAATAATAAAAACAA
CAACAACAAAAATAATATAACAATAATCTTTTATTTTTTTTTATTAATATAAATTAAAAA
AAAAAXXXXXXXXXXGCAGCATCAAAATCAGATTCCACTTTGGATGCATTAAACATTTAC
AATGGTCATACATCAATTGTTGAAGATGTAGCATGGCATTATATTCACGATACATTTTTT
GGATCAGTAGGCGATGATAAGAAACTTATGATTTGGGATACTCGTACTGGAACAAAACCA
ATTCACGTTGTTGAAGCACATAATAGCGAAGTCAATTGTTTATCATTCAATCCATTTTGT
GAGTTTTTAGTTGCAACTGGTTCAACCGATAAGACCGTTGCTCTTTGGGATATGCGTAAT
TTAGGTAATCGTCTTCATTCTTTAATCTCTCATACTGATGAAGTGTTTCAAGTTCAATTT
TCACCACATAATGAAACGGTTTTGGCAAGTTGTGGTTCTGATCGTCGTGTAAACGTTTGG
GATCTCTCTAGAATAGGTGAAGAACAAAACAATG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
finkiyayiqivl*iiikttttkii*q*sfiffy*YKLKKK---

---AASKSDSTLDALNIYNGHTSIVEDVAWHYIHDTFFGSVGDDKKLMIWDTRTGTKPIH
VVEAHNSEVNCLSFNPFCEFLVATGSTDKTVALWDMRNLGNRLHSLISHTDEVFQVQFSP
HNETVLASCGSDRRVNVWDLSRIGEEQNN
Translated Amino Acid sequence (All Frames) 
Frame A:
nl*ikymhifk*fyk***kqqqqk*ynnnllfffinin*kk---

---AASKSDSTLDALNIYNGHTSIVEDVAWHYIHDTFFGSVGDDKKLMIWDTRTGTKPIH
VVEAHNSEVNCLSFNPFCEFLVATGSTDKTVALWDMRNLGNRLHSLISHTDEVFQVQFSP
HNETVLASCGSDRRVNVWDLSRIGEEQNN

Frame B:
iyk*niciysnsfinnnknnnnknnitiifyfflli*ikkk---

---qhqnqiplwmh*tftmvihqllkm*hgiiftihfldq*amirnl*fgilvleqnqft
llkhiiaksivyhsihfvsf*lqlvqpirpllfgicvi*vivfil*slilmkcfkfnfhh
imkrfwqvvvlivv*tfgisle*vknktm

Frame C:
finkiyayiqivl*iiikttttkii*q*sfiffy*YKLKKK---

---sikirfhfgcikhlqwsyinc*rcsmalysryifwisrr**etydlgysywnktnsr
c*st**rsqlfiiqsil*vfscnwfnr*drcslgya*fr*sssffnlsy**svsssiftt
**ngfgklwf*ssckrlgsl*nr*rtkq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ175 (VFJ175Q) /CSM/VF/VFJ1-D/VFJ175Q.Seq.d/                        870   0.0
AFL441 (AFL441Q) /CSM/AF/AFL4-B/AFL441Q.Seq.d/                        870   0.0
AFL229 (AFL229Q) /CSM/AF/AFL2-B/AFL229Q.Seq.d/                        870   0.0
AFI439 (AFI439Q) /CSM/AF/AFI4-B/AFI439Q.Seq.d/                        868   0.0
AFF147 (AFF147Q) /CSM/AF/AFF1-B/AFF147Q.Seq.d/                        862   0.0
VFD507 (VFD507Q) /CSM/VF/VFD5-A/VFD507Q.Seq.d/                        702   0.0
AFA262 (AFA262Q) /CSM/AF/AFA2-C/AFA262Q.Seq.d/                        563   e-159
AFD468 (AFD468Q) /CSM/AF/AFD4-C/AFD468Q.Seq.d/                        422   e-117
AFD891 (AFD891Q) /CSM/AF/AFD8-D/AFD891Q.Seq.d/                         86   7e-16
SFL265 (SFL265Q) /CSM/SF/SFL2-C/SFL265Q.Seq.d/                         56   6e-07
own update 2001.11.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CD074099|CD074099.1 MA3-0001U-M280-B08-U.G MA3-0001 Schistosoma mansoni cDNA cloneMA3-0001U-M280-B08.G, mRNA sequence.    46   4e-12  3
BI862961|BI862961.1 rm14f05.y1 Meloidogyne arenaria egg pAMP1 v1 Chiapelli McCarter Meloidogyne arenaria cDNA 5' similar to SW:RB48_HUMAN Q09028 CHROMATIN ASSEMBLY FACTOR 1 P48 SUBUNIT ;, mRNA sequence.    50   2e-11  3
CD079902|CD079902.1 MA3-9999U-M280-B08-U.B MA3-0001 Schistosoma mansoni cDNA clone MA3-9999U-M280-B08.B, mRNA sequence.    52   2e-11  3
BU794126|CAF1_DROME CHROMATIN ASSEMBLY FACTOR 1 P55 SUBUNIT (CAF-1 P55 SUBUNIT) (DCAF-1) (NUCLEOSOME REMODELING FACTOR 55 KDA SUBUNIT) (NURF-55), mRNA sequence.    50   2e-07  3
CN756145|CN756145.1 ID0AAA17DE04RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA17DE04 5', mRNA sequence.    42   2e-06  4
BI745989|BI745989.1 rm21d02.y1 Meloidogyne arenaria egg pAMP1 v1 Chiapelli McCarter Meloidogyne arenaria cDNA 5' similar to SW:RB46_HUMAN Q16576 HISTONE ACETYLTRANSFERASE TYPE B SUBUNIT 2 ;, mRNA sequence.    44   2e-05  2
BE779693|BE779693.1 601465216F1 NIH_MGC_67 Homo sapiens cDNA clone IMAGE:3868659 5', mRNA sequence.    60   4e-05  1
AE000658|AE000658.1 Homo sapiens T-cell receptor alpha delta locus from bases 1 to 250529 (section 1 of 5) of the Complete Nucleotide Sequence.    58   2e-04  1
BG282998|BG282998.1 602406187F1 NIH_MGC_91 Homo sapiens cDNA clone IMAGE:4518356 5', mRNA sequence.    58   2e-04  1
CK232366|CK232366.1 ILLUMIGEN_MCQ_4000 Katze_MMPL2 Macaca mulatta cDNA 5' similar to human RBBP4 (Hs.16003), mRNA sequence.    58   2e-04  1
dna update 2004. 8. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SD4) RecName: Full=Probable histone-binding protein rbbD;         306   2e-82
(Q24572) RecName: Full=Probable histone-binding protein Caf1; Al...   214   2e-54
BT045583_1(BT045583|pid:none) Salmo salar clone ssal-rgf-525-298...   212   5e-54
BC077257_1(BC077257|pid:none) Xenopus laevis retinoblastoma A as...   212   6e-54
(Q9W7I5) RecName: Full=Histone-binding protein RBBP4; AltName: F...   212   6e-54
AK299251_1(AK299251|pid:none) Homo sapiens cDNA FLJ53908 complet...   212   6e-54
AK222779_1(AK222779|pid:none) Homo sapiens mRNA for retinoblasto...   212   6e-54
(O93377) RecName: Full=Histone-binding protein RBBP4-A; AltName:...   212   6e-54
(Q09028) RecName: Full=Histone-binding protein RBBP4; AltName: F...   212   6e-54
(Q5M7K4) RecName: Full=Histone-binding protein RBBP4; AltName: F...   212   6e-54
protein update 2009. 4.25
PSORT 

 psg: 0.66  gvh: 0.36  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFL229 is cyt
5' end seq. ID AFL229F
5' end seq. 
>AFL229F.Seq
AATTTATAAATAAAATATATGCATATATTCAAATAGTTTTATAAATAATAATAAAAACAA
CAACAACAAAAATAATATAACAATAATCTTTTATTTTTTTTTATTAATATAAATTAAAAA
AAAAA----------
Length of 5' end seq. 125
3' end seq. ID AFL229Z
3' end seq. 
>AFL229Z.Seq
----------GCAGCATCAAAATCAGATTCCACTTTGGATGCATTAAACATTTACAATGG
TCATACATCAATTGTTGAAGATGTAGCATGGCATTATATTCACGATACATTTTTTGGATC
AGTAGGCGATGATAAGAAACTTATGATTTGGGATACTCGTACTGGAACAAAACCAATTCA
CGTTGTTGAAGCACATAATAGCGAAGTCAATTGTTTATCATTCAATCCATTTTGTGAGTT
TTTAGTTGCAACTGGTTCAACCGATAAGACCGTTGCTCTTTGGGATATGCGTAATTTAGG
TAATCGTCTTCATTCTTTAATCTCTCATACTGATGAAGTGTTTCAAGTTCAATTTTCACC
ACATAATGAAACGGTTTTGGCAAGTTGTGGTTCTGATCGTCGTGTAAACGTTTGGGATCT
CTCTAGAATAGGTGAAGAACAAAACAATG
Length of 3' end seq. 439
Connected seq. ID AFL229P
Connected seq. 
>AFL229P.Seq
AATTTATAAATAAAATATATGCATATATTCAAATAGTTTTATAAATAATAATAAAAACAA
CAACAACAAAAATAATATAACAATAATCTTTTATTTTTTTTTATTAATATAAATTAAAAA
AAAAA----------GCAGCATCAAAATCAGATTCCACTTTGGATGCATTAAACATTTAC
AATGGTCATACATCAATTGTTGAAGATGTAGCATGGCATTATATTCACGATACATTTTTT
GGATCAGTAGGCGATGATAAGAAACTTATGATTTGGGATACTCGTACTGGAACAAAACCA
ATTCACGTTGTTGAAGCACATAATAGCGAAGTCAATTGTTTATCATTCAATCCATTTTGT
GAGTTTTTAGTTGCAACTGGTTCAACCGATAAGACCGTTGCTCTTTGGGATATGCGTAAT
TTAGGTAATCGTCTTCATTCTTTAATCTCTCATACTGATGAAGTGTTTCAAGTTCAATTT
TCACCACATAATGAAACGGTTTTGGCAAGTTGTGGTTCTGATCGTCGTGTAAACGTTTGG
GATCTCTCTAGAATAGGTGAAGAACAAAACAATG
Length of connected seq. 564
Full length Seq ID -
Full length Seq. -
Length of full length seq. -