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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL225
Library AF
(Link to library)
Clone ID AFL225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13885-1
Original site URL
Representative seq. ID AFL225P
(Link to Original site)
Representative DNA sequence 
>AFL225 (AFL225Q) /CSM/AF/AFL2-B/AFL225Q.Seq.d/
AATTTTATTTTATTTTACACACACTCACATAAATATACATATTTAAATAATAATAATAAA
TAAAAAAAAATAAAAAAATGAAAAAACAAATATTTTTTATTATTTTACTTTTTACATTTT
TATTTTTTATTAATAATATAGAATCAAAGAATAATAGTGTACTTGGAAATTGTGAAAAGA
ATCAAACTGCAAAATATAAAAAGATATCATCCAAATATGGTAATGAAGAAATTAAAGAGA
TTACAACCAATCAATTAAGCAAAGATCAACTTGAAAAACATGAAATGTTTATGAAGATTG
CATTGGAAATAGCAATTAAAAGTAAAGTTAAATTCGTTTCCATCATTGTATCACCAGATG
ATCGTGTACTTTGTTCAGGTATCTTCTCAAAAGAGAATGCCATTCTTCATAGTGAATTGG
TTGCCATTACAAATTGCAGTTCGTCCCATAAAATGGTAACTTTTGAAAATCATACAATCT
ATAGTACTGCCGAACCAGATXXXXXXXXXXCAATGTAAATGTAAAAATCCAAAATTATCA
AAAGAAAATACCANCAACAACGATGATGGTGATGATGATAACAAAAACCATCATCGTCGT
CATAGTCAAAATAATGAAATTGATGTTGATACTAAAAATGAAAGAAATCAACACCACCAT
GACAAAGATGATAGTATTGATAACTTTTTCGATGAAATAAAATCTGATGATGATTTTGAT
AAAAGAATTGAATCAGGTAAACAAGCTGAAAAGTCAAAGAATAAAGAAAAAAAGAAACAT
CATTCTCACCATCACTCTCACACTAGTAAAGATGGTCGTAGTAGAACAAGTACAAGTACA
AGTATTTCAGGTAATGGTCATGCAACTGCTTCTGCCTCTGCTTCTTCTTCTTCTTCTTCT
ACTTCTTTCTCTCATTCTTCCTCTTCAAATGATCATCACCATAAAAAGGATAAATCAAAT
AAAAACAAAGACAACAAAGAAAAAGGTNATAAAAAAGATAAAAAAGATAAAAAAGAAAAG
AAAGATAAGAAAAAAGATAAAAAAGATAAATCAACCTCTGTAGAAGAAGAAATCAATACC
TTAAAAGTCGAAAAGAATTCAATTTAAATTTATTAAATAATTTAAAGNT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fyfilhtlt*iyifk****IKKNKKMKKQIFFIILLFTFLFFINNIESKNNSVLGNCEKN
QTAKYKKISSKYGNEEIKEITTNQLSKDQLEKHEMFMKIALEIAIKSKVKFVSIIVSPDD
RVLCSGIFSKENAILHSELVAITNCSSSHKMVTFENHTIYSTAEPD---

---QCKCKNPKLSKENTXNNDDGDDDNKNHHRRHSQNNEIDVDTKNERNQHHHDKDDSID
NFFDEIKSDDDFDKRIESGKQAEKSKNKEKKKHHSHHHSHTSKDGRSRTSTSTSISGNGH
ATASASASSSSSSTSFSHSSSSNDHHHKKDKSNKNKDNKEKGXKKDKKDKKEKKDKKKDK
KDKSTSVEEEINTLKVEKNSI*iy*ii*x
Translated Amino Acid sequence (All Frames) 
Frame A:
nfilfythshkytylnnnnk*kkikk*knkyfllfyflhfyfllii*nqriivyleivkr
iklqnikryhpnmvmkklkrlqpin*akinlknmkcl*rlhwk*qlkvklnsfpslyhqm
ivyfvqvssqkrmpffivnwlplqiavrpikw*llkiiqsivlpnq---

---QCKCKNPKLSKENTXNNDDGDDDNKNHHRRHSQNNEIDVDTKNERNQHHHDKDDSID
NFFDEIKSDDDFDKRIESGKQAEKSKNKEKKKHHSHHHSHTSKDGRSRTSTSTSISGNGH
ATASASASSSSSSTSFSHSSSSNDHHHKKDKSNKNKDNKEKGXKKDKKDKKEKKDKKKDK
KDKSTSVEEEINTLKVEKNSI*iy*ii*x

Frame B:
ilfyfththinihi*iiiinkkk*knektnifyyftfyifify**yrike**ctwkl*ke
sncki*kdiiqiw**rn*rdynqsikqrst*kt*nvyedcignsn*k*s*irfhhcitr*
sctlfryllkrechss**igchyklqfvp*ngnf*ksynl*ycrtr---

---nvnvkiqnyqkkipxttmmvmmitktiivvivkimklmlilkmkeinttmtkmivli
tfsmk*nlmmilikelnqvnklksqrikkkrniiltitltlvkmvvveqvqvqvfqvmvm
qlllpllllllllllslilplqmiitikrinqiktkttkkkvikkikkikkkrkirkkik
kinqpl*kkksip*kskriqfkfik*fkx

Frame C:
fyfilhtlt*iyifk****IKKNKKMKKQIFFIILLFTFLFFINNIESKNNSVLGNCEKN
QTAKYKKISSKYGNEEIKEITTNQLSKDQLEKHEMFMKIALEIAIKSKVKFVSIIVSPDD
RVLCSGIFSKENAILHSELVAITNCSSSHKMVTFENHTIYSTAEPD---

---m*m*kskiikrkyxqqr*w****qkpssss*sk**n*c*y*k*kkstpp*qr**y**
lfr*nki***f**kn*ir*ts*kvke*rkketsfspslsh**rws**nkykykyfr*wsc
ncfclcffffffyfflsfflfk*ssp*kg*ik*kqrqqrkrx*kr*kr*krker*ekr*k
r*inlcrrrnqylksrkefnlnllnnlk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL225 (AFL225Q) /CSM/AF/AFL2-B/AFL225Q.Seq.d/                       1348   0.0
SFJ787 (SFJ787Q) /CSM/SF/SFJ7-D/SFJ787Q.Seq.d/                        729   0.0
SFJ362 (SFJ362Q) /CSM/SF/SFJ3-C/SFJ362Q.Seq.d/                        729   0.0
SFG631 (SFG631Q) /CSM/SF/SFG6-B/SFG631Q.Seq.d/                        729   0.0
CHE494 (CHE494Q) /CSM/CH/CHE4-D/CHE494Q.Seq.d/                        729   0.0
AFK271 (AFK271Q) /CSM/AF/AFK2-C/AFK271Q.Seq.d/                        729   0.0
AFJ852 (AFJ852Q) /CSM/AF/AFJ8-C/AFJ852Q.Seq.d/                        729   0.0
AFJ609 (AFJ609Q) /CSM/AF/AFJ6-A/AFJ609Q.Seq.d/                        729   0.0
AFE746 (AFE746Q) /CSM/AF/AFE7-B/AFE746Q.Seq.d/                        729   0.0
SLC561 (SLC561Q) /CSM/SL/SLC5-C/SLC561Q.Seq.d/                        714   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          38   3e-08  26
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   2e-06  19
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   3e-06  24
AF090533|AF090533.1 Dictyostelium discoideum unconventional myosin heavy chain MyoM (myoM) mRNA, complete cds.    38   4e-05  6
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   6e-05  17
AB017910|AB017910.1 Dictyostelium discoideum myoM gene, complete cds.    38   9e-05  6
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   1e-04  17
AE014839|AE014839.1 Plasmodium falciparum 3D7 chromosome 11 section 4 of 8 of the complete sequence.    44   1e-04  14
AL929354|AL929354.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 4/4.    34   2e-04  16
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36   3e-04  12
dna update 2004. 8. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116977_84(AC116977|pid:none) Dictyostelium discoideum chromoso...    72   4e-11
AC116032_1(AC116032|pid:none) Dictyostelium discoideum chromosom...    64   7e-09
T31613(T31613)hypothetical protein Y50E8A.i - Caenorhabditis ele...    43   0.017
CP000634_69(CP000634|pid:none) Agrobacterium vitis S4 chromosome...    37   1.2
AE001362_162(AE001362|pid:none) Plasmodium falciparum 3D7 chromo...    37   1.2
AY714675_1(AY714675|pid:none) Epizootic hemorrhagic disease viru...    35   4.6
AY714694_1(AY714694|pid:none) Epizootic hemorrhagic disease viru...    35   4.6
AL844508_99(AL844508|pid:none) Plasmodium falciparum strain 3D7,...    35   4.6
CR382399_68(CR382399|pid:none) Plasmodium falciparum chromosome ...    35   4.6
AP009484_1852(AP009484|pid:none) Macrococcus caseolyticus JCSC54...    35   5.9
protein update 2009. 4.25
PSORT 

 psg: 1.02  gvh: 0.60  alm: 0.21  top: 0.07  tms: 0.07  mit: 0.27  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for AFL225 is cyt
5' end seq. ID AFL225F
5' end seq. 
>AFL225F.Seq
AATTTTATTTTATTTTACACACACTCACATAAATATACATATTTAAATAATAATAATAAA
TAAAAAAAAATAAAAAAATGAAAAAACAAATATTTTTTATTATTTTACTTTTTACATTTT
TATTTTTTATTAATAATATAGAATCAAAGAATAATAGTGTACTTGGAAATTGTGAAAAGA
ATCAAACTGCAAAATATAAAAAGATATCATCCAAATATGGTAATGAAGAAATTAAAGAGA
TTACAACCAATCAATTAAGCAAAGATCAACTTGAAAAACATGAAATGTTTATGAAGATTG
CATTGGAAATAGCAATTAAAAGTAAAGTTAAATTCGTTTCCATCATTGTATCACCAGATG
ATCGTGTACTTTGTTCAGGTATCTTCTCAAAAGAGAATGCCATTCTTCATAGTGAATTGG
TTGCCATTACAAATTGCAGTTCGTCCCATAAAATGGTAACTTTTGAAAATCATACAATCT
ATAGTACTGCCGAACCAGAT----------
Length of 5' end seq. 500
3' end seq. ID AFL225Z
3' end seq. 
>AFL225Z.Seq
----------CAATGTAAATGTAAAAATCCAAAATTATCAAAAGAAAATACCANCAACAA
CGATGATGGTGATGATGATAACAAAAACCATCATCGTCGTCATAGTCAAAATAATGAAAT
TGATGTTGATACTAAAAATGAAAGAAATCAACACCACCATGACAAAGATGATAGTATTGA
TAACTTTTTCGATGAAATAAAATCTGATGATGATTTTGATAAAAGAATTGAATCAGGTAA
ACAAGCTGAAAAGTCAAAGAATAAAGAAAAAAAGAAACATCATTCTCACCATCACTCTCA
CACTAGTAAAGATGGTCGTAGTAGAACAAGTACAAGTACAAGTATTTCAGGTAATGGTCA
TGCAACTGCTTCTGCCTCTGCTTCTTCTTCTTCTTCTTCTACTTCTTTCTCTCATTCTTC
CTCTTCAAATGATCATCACCATAAAAAGGATAAATCAAATAAAAACAAAGACAACAAAGA
AAAAGGTNATAAAAAAGATAAAAAAGATAAAAAAGAAAAGAAAGATAAGAAAAAAGATAA
AAAAGATAAATCAACCTCTGTAGAAGAAGAAATCAATACCTTAAAAGTCGAAAAGAATTC
AATTTAAATTTATTAAATAATTTAAAGNT
Length of 3' end seq. 619
Connected seq. ID AFL225P
Connected seq. 
>AFL225P.Seq
AATTTTATTTTATTTTACACACACTCACATAAATATACATATTTAAATAATAATAATAAA
TAAAAAAAAATAAAAAAATGAAAAAACAAATATTTTTTATTATTTTACTTTTTACATTTT
TATTTTTTATTAATAATATAGAATCAAAGAATAATAGTGTACTTGGAAATTGTGAAAAGA
ATCAAACTGCAAAATATAAAAAGATATCATCCAAATATGGTAATGAAGAAATTAAAGAGA
TTACAACCAATCAATTAAGCAAAGATCAACTTGAAAAACATGAAATGTTTATGAAGATTG
CATTGGAAATAGCAATTAAAAGTAAAGTTAAATTCGTTTCCATCATTGTATCACCAGATG
ATCGTGTACTTTGTTCAGGTATCTTCTCAAAAGAGAATGCCATTCTTCATAGTGAATTGG
TTGCCATTACAAATTGCAGTTCGTCCCATAAAATGGTAACTTTTGAAAATCATACAATCT
ATAGTACTGCCGAACCAGAT----------CAATGTAAATGTAAAAATCCAAAATTATCA
AAAGAAAATACCANCAACAACGATGATGGTGATGATGATAACAAAAACCATCATCGTCGT
CATAGTCAAAATAATGAAATTGATGTTGATACTAAAAATGAAAGAAATCAACACCACCAT
GACAAAGATGATAGTATTGATAACTTTTTCGATGAAATAAAATCTGATGATGATTTTGAT
AAAAGAATTGAATCAGGTAAACAAGCTGAAAAGTCAAAGAATAAAGAAAAAAAGAAACAT
CATTCTCACCATCACTCTCACACTAGTAAAGATGGTCGTAGTAGAACAAGTACAAGTACA
AGTATTTCAGGTAATGGTCATGCAACTGCTTCTGCCTCTGCTTCTTCTTCTTCTTCTTCT
ACTTCTTTCTCTCATTCTTCCTCTTCAAATGATCATCACCATAAAAAGGATAAATCAAAT
AAAAACAAAGACAACAAAGAAAAAGGTNATAAAAAAGATAAAAAAGATAAAAAAGAAAAG
AAAGATAAGAAAAAAGATAAAAAAGATAAATCAACCTCTGTAGAAGAAGAAATCAATACC
TTAAAAGTCGAAAAGAATTCAATTTAAATTTATTAAATAATTTAAAGNT
Length of connected seq. 1119
Full length Seq ID -
Full length Seq. -
Length of full length seq. -