>AFL224 (AFL224Q) /CSM/AF/AFL2-A/AFL224Q.Seq.d/
XXXXXXXXXXTTTNTGATACAAAGGATTTAGGTGAAGATATAAATATAGCNCAAGTACCA
TATGAATTGCCAGATGGTAATTTATTANAGATTGGTTCAGATAGATTTCAAATTCCTGAA
TTATTATTTAATCCAACACCATTGAATGAACAAATTTTAAATAACAATAATAATAATAGC
AATGGTGAGAATACAACAACATCATCAGTTGTTCAACAACCNCAATATCAAGGATTAGGA
AGAATGATTATAGAATCATTANAGAAAAGTGATACAGATATTCGTAAAGAATTAATAAGT
AATTTAATTATAACTGGTGGTAATACATTATTTGCAGGTTTCCAAGAAAGATTAGTTANA
GACATTTCTGAAATCTCTTATAATAAACCAAAAATCGTTGCAAATCAAACTGAAAAAAAG
AATTCAGTTTGGATTGG

---XDTKDLGEDINIAQVPYELPDGNLLXIGSDRFQIPELLFNPTPLNEQILNNNNNNSN
GENTTTSSVVQQPQYQGLGRMIIESLXKSDTDIRKELISNLIITGGNTLFAGFQERLVXD
ISEISYNKPKIVANQTEKKNSVWI

Frame A:
---x*ykgfr*rykysxsti*iarw*fixdwfr*isns*iii*sntie*tnfk*q****q
w*eynniiscsttxisrirkndyriixek*yrys*rink*fnynww*yiicrfprkisxr
hf*nll**tknrcksn*kkefsldw


Frame B:
---XDTKDLGEDINIAQVPYELPDGNLLXIGSDRFQIPELLFNPTPLNEQILNNNNNNSN
GENTTTSSVVQQPQYQGLGRMIIESLXKSDTDIRKELISNLIITGGNTLFAGFQERLVXD
ISEISYNKPKIVANQTEKKNSVWI


Frame C:
---xiqri*vki*i*xkyhmncqmviyxrlvqidfkflnyyliqhh*mnkf*itiiiiam
vriqqhhqlfnnxnikd*ee*l*nhxrkviqifvkn**vi*l*lvvihylqvskkd*lxt
flksliinqkslqiklkkriqfgl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL243 (SFL243Q) /CSM/SF/SFL2-B/SFL243Q.Seq.d/                        769   0.0
SFB730 (SFB730Q) /CSM/SF/SFB7-B/SFB730Q.Seq.d/                        769   0.0
CFH524 (CFH524Q) /CSM/CF/CFH5-A/CFH524Q.Seq.d/                        769   0.0
AFL224 (AFL224Q) /CSM/AF/AFL2-A/AFL224Q.Seq.d/                        769   0.0
AFG296 (AFG296Q) /CSM/AF/AFG2-D/AFG296Q.Seq.d/                        769   0.0
SFC783 (SFC783Q) /CSM/SF/SFC7-D/SFC783Q.Seq.d/                        763   0.0
SFF737 (SFF737Q) /CSM/SF/SFF7-B/SFF737Q.Seq.d/                        761   0.0
AFM734 (AFM734Q) /CSM/AF/AFM7-B/AFM734Q.Seq.d/                        761   0.0
CFK273 (CFK273Q) /CSM/CF/CFK2-D/CFK273Q.Seq.d/                        745   0.0
AFA162 (AFA162Q) /CSM/AF/AFA1-C/AFA162Q.Seq.d/                        630   e-180

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014838|AE014838.1 Plasmodium falciparum 3D7 chromosome 11 section 3 of 8 of the complete sequence.    38   1e-06  11
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    48   2e-06  3
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    46   5e-06  4
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    44   1e-05  3
AE014826|AE014826.1 Plasmodium falciparum 3D7 chromosome 14 section 11 of 13 of the complete sequence.    38   2e-05  12
AY484462|AY484462.1 Dictyostelium discoideum kinesin family member 8 gene, complete cds.    38   2e-05  4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    46   2e-05  4
Z98551|Z98551.1 Plasmodium falciparum MAL3P6.                      42   4e-05  7
AC004688|AC004688.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    40   7e-05  9
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    40   7e-05  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY763944_1(AY763944|pid:none) Allogromia sp. A clone Pu_94t acti...    85   6e-16
BT038602_1(BT038602|pid:none) Zea mays full-length cDNA clone ZM...    85   8e-16
(Q84M92) RecName: Full=Actin-related protein 4;  &AK228098_1(AK2...    84   1e-15
AF421536_1(AF421536|pid:none) Crypthecodinium cohnii actin mRNA,...    84   2e-15
AY087788_1(AY087788|pid:none) Arabidopsis thaliana clone 38419 m...    84   2e-15
AJ132370_1(AJ132370|pid:none) Allogromia sp. mRNA for actin 1.         84   2e-15
AC152123_6(AC152123|pid:none) Genomic sequence for broccoli [Bra...    83   2e-15
DQ980427_1(DQ980427|pid:none) Peridinium limbatum clone plactin2...    83   3e-15
(P45520) RecName: Full=Actin;  &L16961_1(L16961|pid:none)              82   4e-15
AY079087_1(AY079087|pid:none) Leishmania donovani actin gene, co...    82   4e-15

 psg: 0.75  gvh: 0.27  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.11  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFL224 is cyt

>AFL224Z.Seq
----------TTTNTGATACAAAGGATTTAGGTGAAGATATAAATATAGCNCAAGTACCA
TATGAATTGCCAGATGGTAATTTATTANAGATTGGTTCAGATAGATTTCAAATTCCTGAA
TTATTATTTAATCCAACACCATTGAATGAACAAATTTTAAATAACAATAATAATAATAGC
AATGGTGAGAATACAACAACATCATCAGTTGTTCAACAACCNCAATATCAAGGATTAGGA
AGAATGATTATAGAATCATTANAGAAAAGTGATACAGATATTCGTAAAGAATTAATAAGT
AATTTAATTATAACTGGTGGTAATACATTATTTGCAGGTTTCCAAGAAAGATTAGTTANA
GACATTTCTGAAATCTCTTATAATAAACCAAAAATCGTTGCAAATCAAACTGAAAAAAAG
AATTCAGTTTGGATTGG