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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL221
Library AF
(Link to library)
Clone ID AFL221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14949-1
Original site URL
Representative seq. ID AFL221P
(Link to Original site)
Representative DNA sequence 
>AFL221 (AFL221Q) /CSM/AF/AFL2-A/AFL221Q.Seq.d/
ATAAAAATGATTTNTTAGAAGATCTATCACCAACATTATGGAAATAACCAGATAAAGAGG
GTGAATTAAAGAAACAAGGACATGTAGTAAAGAATTGGAAGAAAAGAAAGTCTATTATTC
AAAATGATATGTTATTTTATTTTAAGGATAAAGAGGAGAGACCAGTTGGAGCGGTGCCAT
TAAGAATGTCACGTTGCTATGAAAATAAATCATTAGGTAAACCAAATTGTTTCGAATTAG
TATCACCAAGAATCAATAAAACATTTTTCATTCAAGCCAATACACCAGATGAAATGGCAA
GTTGGATGAAAGCAGTTGAGAAAGGATCAGAATATAGCACAGTATCACAACCATTCAATC
TTAAACATGAGGTACATGTTGATTTCAATTCAGCAACTGGTTTCTCTGGTTTACCAAAAG
AATGGGAAGTTATCTTAAAGTCTTCAAATGTTTCAAAACAAGAAGTACTCGATAAACCAA
GTGAATGGTTATCAGTTTTAGAATTTCAAGCTGGTAGAACAATGGAAAAATCAAATTCTC
AAAATTTATCTGCACTTCCTGATGAATCAAATTTAACTCTTAGTGATTTAGTAACXXXXX
XXXXXCTTTGGGTTGCAATGGAGTTTATGGGTGGTGGTTGTTTAACAGACATTTTAGAGG
CATTTGATAATATCAAAATGAGTGAGATTCAAATTGCTTATGTGGTTAAAGAGACCTTGA
AGGCATTGCAATACATTCATAGTCTTCATCGTATTCATAGAGATATAAAGAGTGATAATA
TTTTATTGGGCTCAGAGGGTAGTGTAAAGATTGCTGATTTTGGTTATGCCGCTCAATTAA
CTCAAAAACAACAAAAACGTAATACCGTCGTTGGTACCCCTTATTGGATGGCCCCAGAAC
TCATTAGAGGTCACGATTATGGTGTCAAGGTTGATATTTGGTCTTTGGGTATCATGATGA
TGGAAATGGCTGAAGGTGAACCACCTTATATGGATTTCCCACCATTGCGTGCTCTTTTCC
TCATCACTACTAAAGGTATTCCACCACTTAAAGAAACAACCAAATGGAGTAAAACTTTCC
AAGATTTCTTCTCAAAATGTCTCGATATCAATGTTGCTAATCGTCCTGATGCTACCGATC
TTTTAAAACATCCTTTCATGGATCTGGCATGTATTCCTCNAATTTAACCATNATTCAAGC
CGCAAGAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kndxledlsptlwk*PDKEGELKKQGHVVKNWKKRKSIIQNDMLFYFKDKEERPVGAVPL
RMSRCYENKSLGKPNCFELVSPRINKTFFIQANTPDEMASWMKAVEKGSEYSTVSQPFNL
KHEVHVDFNSATGFSGLPKEWEVILKSSNVSKQEVLDKPSEWLSVLEFQAGRTMEKSNSQ
NLSALPDESNLTLSDLV---

---LWVAMEFMGGGCLTDILEAFDNIKMSEIQIAYVVKETLKALQYIHSLHRIHRDIKSD
NILLGSEGSVKIADFGYAAQLTQKQQKRNTVVGTPYWMAPELIRGHDYGVKVDIWSLGIM
MMEMAEGEPPYMDFPPLRALFLITTKGIPPLKETTKWSKTFQDFFSKCLDINVANRPDAT
DLLKHPFMDLACIPXI*pxfkpqe
Translated Amino Acid sequence (All Frames) 
Frame A:
ikmix*kiyhqhygnnqikrvn*rnkdm**rigrkesllfkmicyfilrikrrdqlerch
*echvamkinh*vnqivsn*yhqesikhfsfkpihqmkwqvg*kqlrkdqniaqyhnhsi
lnmrymlisiqqlvslvyqkngkls*slqmfqnkkysinqvngyqf*nfklveqwknqil
kiylhflmnqi*llvi**---

---LWVAMEFMGGGCLTDILEAFDNIKMSEIQIAYVVKETLKALQYIHSLHRIHRDIKSD
NILLGSEGSVKIADFGYAAQLTQKQQKRNTVVGTPYWMAPELIRGHDYGVKVDIWSLGIM
MMEMAEGEPPYMDFPPLRALFLITTKGIPPLKETTKWSKTFQDFFSKCLDINVANRPDAT
DLLKHPFMDLACIPXI*pxfkpqe

Frame B:
*k*fxrrsitnimeitr*rg*iketrtcskeleekkvyysk*yvilf*g*rgetswsgai
knvtll*k*iir*tklfrisitknq*nifhssqytr*ngkldess*eriri*hsittiqs
*t*gtc*fqfsnwflwftkrmgsylkvfkcfktrstr*tk*mvisfrissw*nngkikfs
kficts**ikfns**fsn---

---fglqwslwvvvv*qtf*rhliisk*vrfkllmwlkrp*rhcntfivfivfiei*rvi
ifywaqrvv*rllilvmpln*lknnknvipslvpligwpqnslevtimvsrlifglwvs*
*wkwlkvnhliwishhcvlfsssllkvfhhlkkqpngvklskissqnvsismllivlmlp
if*nilswiwhvflxfnhxssrkk

Frame C:
kndxledlsptlwk*PDKEGELKKQGHVVKNWKKRKSIIQNDMLFYFKDKEERPVGAVPL
RMSRCYENKSLGKPNCFELVSPRINKTFFIQANTPDEMASWMKAVEKGSEYSTVSQPFNL
KHEVHVDFNSATGFSGLPKEWEVILKSSNVSKQEVLDKPSEWLSVLEFQAGRTMEKSNSQ
NLSALPDESNLTLSDLV---

---lgcngvygwwlfnrhfrgi**yqne*dsnclcg*rdlegiaihs*sssys*ryke**
yfiglrg*ckdc*fwlcrsinskttkt*yrrwyplldgprth*rsrlwcqg*ylvfgyhd
dgng*r*ttlygfptiacsfphhy*rystt*rnnqme*nfprfllkmsryqcc*ss*cyr
sfktsfhgsgmyssnltxiqaar
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL221 (AFL221Q) /CSM/AF/AFL2-A/AFL221Q.Seq.d/                       2319   0.0
SFC672 (SFC672Q) /CSM/SF/SFC6-C/SFC672Q.Seq.d/                       1170   0.0
CFH857 (CFH857Q) /CSM/CF/CFH8-C/CFH857Q.Seq.d/                       1164   0.0
CFA281 (CFA281Q) /CSM/CF/CFA2-D/CFA281Q.Seq.d/                       1164   0.0
SFH221 (SFH221Q) /CSM/SF/SFH2-A/SFH221Q.Seq.d/                       1156   0.0
AFB708 (AFB708Q) /CSM/AF/AFB7-A/AFB708Q.Seq.d/                       1156   0.0
SFL389 (SFL389Q) /CSM/SF/SFL3-D/SFL389Q.Seq.d/                       1154   0.0
AFK585 (AFK585Q) /CSM/AF/AFK5-D/AFK585Q.Seq.d/                       1150   0.0
AFH470 (AFH470Q) /CSM/AF/AFH4-C/AFH470Q.Seq.d/                       1144   0.0
VFF415 (VFF415Q) /CSM/VF/VFF4-A/VFF415Q.Seq.d/                       1136   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF277804|AF277804.3 Dictyostelium discoideum PakC mRNA, complete cds.  1136   0.0    2
U67716|U67716.1 Dictyostelium discoideum myosin I heavy chain kinase (mihck) mRNA, complete cds.    94   8e-49  6
Y10158|Y10158.1 D.discoideum mRNA for STE20/pak kinase homologue.    94   1e-48  6
AC115606|AC115606.2 Dictyostelium discoideum chromosome 2 map 159760-209066 strain AX4, complete sequence.    94   1e-43  9
AF131221|AF131221.1 Dictyostelium discoideum p21-activated protein kinase PakA (pakA) mRNA, complete cds.    66   2e-26  5
U51923|U51923.3 Dictyostelium discoideum STE20-telated kinase DPAK1 (dpak1) mRNA, complete cds.    60   1e-24  5
AZ677365|AZ677365.1 ENTFX50TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   1e-10  5
AZ684578|AZ684578.1 ENTJV90TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   1e-10  5
CR382122|CR382122.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome B of strain NRRL  Y-1140 of Kluyveromyces lactis.    54   2e-08  2
BH161927|BH161927.1 ENTQY64TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42   7e-08  5
dna update 2004. 8. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF277804_1(AF277804|pid:none) Dictyostelium discoideum PakC mRNA...   638   0.0
(Q55GV3) RecName: Full=Serine/threonine-protein kinase pakC;    ...   632   e-180
Y10158_1(Y10158|pid:none) D.discoideum mRNA for STE20/pak kinase...   298   2e-79
(Q869N2) RecName: Full=Serine/threonine-protein kinase pakB;    ...   298   2e-79
U67716_1(U67716|pid:none) Dictyostelium discoideum myosin I heav...   298   2e-79
AY870660_1(AY870660|pid:none) Entamoeba histolytica p21-activate...   292   2e-77
AF131221_1(AF131221|pid:none) Dictyostelium discoideum p21-activ...   273   8e-72
AE014297_446(AE014297|pid:none) Drosophila melanogaster chromoso...   226   9e-58
U56080_1(U56080|pid:none) Drosophila melanogaster p21-activated ...   225   2e-57
AY710430_19(AY710430|pid:none) Cryptococcus gattii strain WM276 ...   225   3e-57
protein update 2009. 4.25
PSORT 

 psg: 0.62  gvh: 0.37  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for AFL221 is cyt
5' end seq. ID AFL221F
5' end seq. 
>AFL221F.Seq
ATAAAAATGATTTNTTAGAAGATCTATCACCAACATTATGGAAATAACCAGATAAAGAGG
GTGAATTAAAGAAACAAGGACATGTAGTAAAGAATTGGAAGAAAAGAAAGTCTATTATTC
AAAATGATATGTTATTTTATTTTAAGGATAAAGAGGAGAGACCAGTTGGAGCGGTGCCAT
TAAGAATGTCACGTTGCTATGAAAATAAATCATTAGGTAAACCAAATTGTTTCGAATTAG
TATCACCAAGAATCAATAAAACATTTTTCATTCAAGCCAATACACCAGATGAAATGGCAA
GTTGGATGAAAGCAGTTGAGAAAGGATCAGAATATAGCACAGTATCACAACCATTCAATC
TTAAACATGAGGTACATGTTGATTTCAATTCAGCAACTGGTTTCTCTGGTTTACCAAAAG
AATGGGAAGTTATCTTAAAGTCTTCAAATGTTTCAAAACAAGAAGTACTCGATAAACCAA
GTGAATGGTTATCAGTTTTAGAATTTCAAGCTGGTAGAACAATGGAAAAATCAAATTCTC
AAAATTTATCTGCACTTCCTGATGAATCAAATTTAACTCTTAGTGATTTAGTAAC-----
-----
Length of 5' end seq. 595
3' end seq. ID AFL221Z
3' end seq. 
>AFL221Z.Seq
----------CTTTGGGTTGCAATGGAGTTTATGGGTGGTGGTTGTTTAACAGACATTTT
AGAGGCATTTGATAATATCAAAATGAGTGAGATTCAAATTGCTTATGTGGTTAAAGAGAC
CTTGAAGGCATTGCAATACATTCATAGTCTTCATCGTATTCATAGAGATATAAAGAGTGA
TAATATTTTATTGGGCTCAGAGGGTAGTGTAAAGATTGCTGATTTTGGTTATGCCGCTCA
ATTAACTCAAAAACAACAAAAACGTAATACCGTCGTTGGTACCCCTTATTGGATGGCCCC
AGAACTCATTAGAGGTCACGATTATGGTGTCAAGGTTGATATTTGGTCTTTGGGTATCAT
GATGATGGAAATGGCTGAAGGTGAACCACCTTATATGGATTTCCCACCATTGCGTGCTCT
TTTCCTCATCACTACTAAAGGTATTCCACCACTTAAAGAAACAACCAAATGGAGTAAAAC
TTTCCAAGATTTCTTCTCAAAATGTCTCGATATCAATGTTGCTAATCGTCCTGATGCTAC
CGATCTTTTAAAACATCCTTTCATGGATCTGGCATGTATTCCTCNAATTTAACCATNATT
CAAGCCGCAAGAAA
Length of 3' end seq. 604
Connected seq. ID AFL221P
Connected seq. 
>AFL221P.Seq
ATAAAAATGATTTNTTAGAAGATCTATCACCAACATTATGGAAATAACCAGATAAAGAGG
GTGAATTAAAGAAACAAGGACATGTAGTAAAGAATTGGAAGAAAAGAAAGTCTATTATTC
AAAATGATATGTTATTTTATTTTAAGGATAAAGAGGAGAGACCAGTTGGAGCGGTGCCAT
TAAGAATGTCACGTTGCTATGAAAATAAATCATTAGGTAAACCAAATTGTTTCGAATTAG
TATCACCAAGAATCAATAAAACATTTTTCATTCAAGCCAATACACCAGATGAAATGGCAA
GTTGGATGAAAGCAGTTGAGAAAGGATCAGAATATAGCACAGTATCACAACCATTCAATC
TTAAACATGAGGTACATGTTGATTTCAATTCAGCAACTGGTTTCTCTGGTTTACCAAAAG
AATGGGAAGTTATCTTAAAGTCTTCAAATGTTTCAAAACAAGAAGTACTCGATAAACCAA
GTGAATGGTTATCAGTTTTAGAATTTCAAGCTGGTAGAACAATGGAAAAATCAAATTCTC
AAAATTTATCTGCACTTCCTGATGAATCAAATTTAACTCTTAGTGATTTAGTAAC-----
-----CTTTGGGTTGCAATGGAGTTTATGGGTGGTGGTTGTTTAACAGACATTTTAGAGG
CATTTGATAATATCAAAATGAGTGAGATTCAAATTGCTTATGTGGTTAAAGAGACCTTGA
AGGCATTGCAATACATTCATAGTCTTCATCGTATTCATAGAGATATAAAGAGTGATAATA
TTTTATTGGGCTCAGAGGGTAGTGTAAAGATTGCTGATTTTGGTTATGCCGCTCAATTAA
CTCAAAAACAACAAAAACGTAATACCGTCGTTGGTACCCCTTATTGGATGGCCCCAGAAC
TCATTAGAGGTCACGATTATGGTGTCAAGGTTGATATTTGGTCTTTGGGTATCATGATGA
TGGAAATGGCTGAAGGTGAACCACCTTATATGGATTTCCCACCATTGCGTGCTCTTTTCC
TCATCACTACTAAAGGTATTCCACCACTTAAAGAAACAACCAAATGGAGTAAAACTTTCC
AAGATTTCTTCTCAAAATGTCTCGATATCAATGTTGCTAATCGTCCTGATGCTACCGATC
TTTTAAAACATCCTTTCATGGATCTGGCATGTATTCCTCNAATTTAACCATNATTCAAGC
CGCAAGAAA
Length of connected seq. 1199
Full length Seq ID -
Full length Seq. -
Length of full length seq. -