>AFL192 (AFL192Q) /CSM/AF/AFL1-D/AFL192Q.Seq.d/
AGTAACTAATTATTCTTCCAAAATAATTAATNATCTCAATGAATTGTCGCCATCACTTTA
TTTAGTGCTTTGACAAATTCATACTTCGAAATATATGTACCAAACAAAATTTGTTGGTTT
GGTGATGTGTTACTGCTGGTGAACACATCCCAAACTGGCTCCATATAAATTACCTTATTA
CTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGATGTATTAAAATCACAAGA
TCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAATTGAAAAATTACC
ATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAACTTTGAAGTACA
CGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAAAGTTGAAATTCC
TTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGCAGTTGTTGATCT
TGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAAAATTAACCCACT
TGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATXXXXXXXXXXATA
GTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTC
GTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAG
GTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGG
CTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAG
ATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATT
ATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGT
GTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTA
TCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAAC
AATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGG
TTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAG
TTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATAATT
TCTTTTTTAAANAAAAAAAAAAAANAA

vtnysskiinxlnelspslylvl*qihtskymyqtkfvglvmcycw*THPKLAPYKLPYY
LKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDNFEVH
EKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAKINPL
VPVDLVIDHSVQVDV---

---SITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIV
NKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQ
GIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQ
TVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLHNFFFKXKKKX

Frame A:
sn*lffqnn*xsq*ivaitlfsaltnsyfeiyvpnkicwfgdvlllvntsqtgsi*itll
lkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgisss*l**l*st
rkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekirw*sskn*pt
css*fsy*slcss*c---

---ivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvl
linwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtf
kelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklv
kp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyiisflxkkkkx

Frame B:
vtnysskiinxlnelspslylvl*qihtskymyqtkfvglvmcycw*THPKLAPYKLPYY
LKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDNFEVH
EKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAKINPL
VPVDLVIDHSVQVDV---

---*yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc
**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsips
rn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nws
nrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktft*flf*xkkkx

Frame C:
*liilpk*lxismncrhhfi*cfdkfilrnictkqnllvw*cvtagehipnwlhinylit
*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqqfvivitlkyt
kkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*vviqqklthl
fqli*llitlfklmy---

---SITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIV
NKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQ
GIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQ
TVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLHNFFFKXKKKX

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL192 (AFL192Q) /CSM/AF/AFL1-D/AFL192Q.Seq.d/                       2454   0.0
VHB318 (VHB318Q) /CSM/VH/VHB3-A/VHB318Q.Seq.d/                       1334   0.0
SHG840 (SHG840Q) /CSM/SH/SHG8-B/SHG840Q.Seq.d/                       1334   0.0
SHG773 (SHG773Q) /CSM/SH/SHG7-D/SHG773Q.Seq.d/                       1334   0.0
CHM637 (CHM637Q) /CSM/CH/CHM6-B/CHM637Q.Seq.d/                       1334   0.0
CHF743 (CHF743Q) /CSM/CH/CHF7-B/CHF743Q.Seq.d/                       1334   0.0
AHM729 (AHM729Q) /CSM/AH/AHM7-B/AHM729Q.Seq.d/                       1330   0.0
AHI823 (AHI823Q) /CSM/AH/AHI8-A/AHI823Q.Seq.d/                       1330   0.0
SHG304 (SHG304Q) /CSM/SH/SHG3-A/SHG304Q.Seq.d/                       1328   0.0
AHN321 (AHN321Q) /CSM/AH/AHN3-A/AHN321Q.Seq.d/                       1328   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115581|AC115581.3 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.   258   1e-72  2
AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    54   1e-30  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    58   1e-25  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    58   4e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   1e-20  3
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   2e-18  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   2e-18  3
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   3e-17  6
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    56   3e-17  4
AF073507|AF073507.1 Citrus limon aconitase-iron regulated protein 1 (IRP1) mRNA, complete cds.    56   4e-14  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   441   e-122
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   289   1e-76
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   288   4e-76
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   286   1e-75
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   285   2e-75
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   285   2e-75
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   282   2e-74
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   281   3e-74
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   281   4e-74
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   280   1e-73

 psg: 0.75  gvh: 0.20  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 36.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFL192 is nuc

>AFL192F.Seq
AGTAACTAATTATTCTTCCAAAATAATTAATNATCTCAATGAATTGTCGCCATCACTTTA
TTTAGTGCTTTGACAAATTCATACTTCGAAATATATGTACCAAACAAAATTTGTTGGTTT
GGTGATGTGTTACTGCTGGTGAACACATCCCAAACTGGCTCCATATAAATTACCTTATTA
CTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGATGTATTAAAATCACAAGA
TCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAATTGAAAAATTACC
ATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAACTTTGAAGTACA
CGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAAAGTTGAAATTCC
TTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGCAGTTGTTGATCT
TGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAAAATTAACCCACT
TGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTAT----------

>AFL192Z.Seq
----------ATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAA
TCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTAT
GGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATT
GTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTA
ATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTG
GCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTT
CAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTT
GGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTC
ACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGT
CAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGAT
ACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAA
CTTTTACATAATTTCTTTTTTAAANAAAAAAAAAAAANAA

>AFL192P.Seq
AGTAACTAATTATTCTTCCAAAATAATTAATNATCTCAATGAATTGTCGCCATCACTTTA
TTTAGTGCTTTGACAAATTCATACTTCGAAATATATGTACCAAACAAAATTTGTTGGTTT
GGTGATGTGTTACTGCTGGTGAACACATCCCAAACTGGCTCCATATAAATTACCTTATTA
CTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGATGTATTAAAATCACAAGA
TCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAATTGAAAAATTACC
ATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAACTTTGAAGTACA
CGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAAAGTTGAAATTCC
TTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGCAGTTGTTGATCT
TGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAAAATTAACCCACT
TGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTAT----------ATA
GTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTC
GTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAG
GTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGG
CTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAG
ATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATT
ATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGT
GTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTA
TCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAAC
AATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGG
TTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAG
TTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATAATT
TCTTTTTTAAANAAAAAAAAAAAANAA