>AFL190 (AFL190Q) /CSM/AF/AFL1-D/AFL190Q.Seq.d/
ATGTTNAAATTTTTTTTAAATTACCCGTCCAAACTTCATTAGTGTCAAAATNAAGATCAA
TTCAATTTAATTATTCAACAAAAAGTAATATTAGAAATATGGAACAATACTTACCACAAG
CTAAGGAATTTATTTCATTTATTGATAAATCACCATCACCATATCATGCAGTTCAATACT
TTTCAGAGATTTTAAAGAGTAAAGGTTTCATTCATTTATCAGAGAAACAAATGTGGGATA
TTCAACCAAATAAAAAATATTTCTTTACTCGTAATCAATCATGTATTTCTGCTTTTGCAG
TTGGTGGTAAATATAAACCAGGTAATGGTTTTAATATTGCAGCAGCACATACTGATAGTC
CAAATTTTAAAGTTAGACCAGTATCAAAAGTTGAATCAGTTGGATATCAACAAGTTGGTG
TTGAAACATATGGTGGTGGATTATGGTATACATGGTTTGATCGTGATTTAACCGTTGCAG
GTAGAGTCATTGTTAAATCAGGTGATGGTAGTTATGAGAGTAAATTAGTTCACATAAAGA
AACCAATTCTTAGAATTCCATCATTAGCAATTCATCTCGATCGXXXXXXXXXXACCAGCT
GCAATCGGTGGTGCACTTGATGAATTCATTTTCTCACCACGTTGTGATAATTTGGGTATG
TCATATTGCGCAATGATTGGTTTATTGAATGTCAAAGAATCAACATTGGCACAAGAGGAG
AATGTATTGAATGTGATATTATTCGATAATGAAGAGGTTGGCTCAAGTTCACCACAAGGT
GCATGTGCACCATTGATCAATGATACCATTAGTCGTGTCAATAGCTCAATGTTTGCATCC
ACTTGTAAACCACATGAATTAAACAATTTCATTGATTTAACTTTAAGAAATAGTTTCTTA
ATCTCTGCTGATATGGCACATGCTATTCATCCAAATTACACAGCAAATCATGAACCACTT
CATAGACCAGCTCTTAATAAAGGTCCAGTAATCAAATATAATGCAAATTTACGTTATGCT
TCAAATGGTCCAACTAGTTTTGTTATCTTGGATATCTGTAAAAAGAATGGTATCCCAATT
CAAGAATTCCTCGTCAAAAATGATTCACCATGTGGTTCAACAATTGGTCCAATCATTTCT
GGTACTTATGGTATTAGAACTGTAGATATTGGTAATCCACAATTATCAATGCATTCAATT
CGTGAAACTTGTGGTGTTGCNGATATCACTCAGGTATNATTNATTCAAAAATATTTNAAC
AATTACAAAAT

VXIFFKLPVQTSLVSKXRSIQFNYSTKSNIRNMEQYLPQAKEFISFIDKSPSPYHAVQYF
SEILKSKGFIHLSEKQMWDIQPNKKYFFTRNQSCISAFAVGGKYKPGNGFNIAAAHTDSP
NFKVRPVSKVESVGYQQVGVETYGGGLWYTWFDRDLTVAGRVIVKSGDGSYESKLVHIKK
PILRIPSLAIHLD---

---PAAIGGALDEFIFSPRCDNLGMSYCAMIGLLNVKESTLAQEENVLNVILFDNEEVGS
SSPQGACAPLINDTISRVNSSMFASTCKPHELNNFIDLTLRNSFLISADMAHAIHPNYTA
NHEPLHRPALNKGPVIKYNANLRYASNGPTSFVILDICKKNGIPIQEFLVKNDSPCGSTI
GPIISGTYGIRTVDIGNPQLSMHSIRETCGVADITQVXXIQKYXNNYK

Frame A:
mxkfflnypsklh*cqnxdqfnliiqqkvileiwnntyhklrnlfhllinhhhhimqfnt
fqrf*rvkvsfiyqrnkcgifnqiknisllvinhvflllqlvvninqvmvlilqqhiliv
qilkldqyqklnqldinklvlkhmvvdygihglivi*plqvesllnqvmvvmrvn*ft*r
nqflefhh*qfisi---

---tscnrwct**ihflttl**fgyvilrndwfiecqrinigtrgeciecdiir**rgwl
kfttrcmctidq*yh*scq*lnvcihl*tt*ikqfh*fnfkk*flnlc*ygtcyssklhs
ks*tts*tss**rssnqi*ckftlcfkwsn*fcylgyl*kewypnsriprqk*ftmwfnn
wsnhfwylwy*ncryw*stiinafns*nlwccxyhsgxixskifxqlqn

Frame B:
cxnff*itrpnfisvkxkinsi*lfnkk*y*kygtiltts*giyfiy**ititiscssil
frdfke*rfhsfiretnvgystk*kiflys*simyfcfcsww*i*tr*wf*ycssty**s
kf*s*tsiks*iswistswc*niwwwimvymv*s*fnrcr*shc*ir*w*l*e*isshke
tns*nsiisnssrs---

---PAAIGGALDEFIFSPRCDNLGMSYCAMIGLLNVKESTLAQEENVLNVILFDNEEVGS
SSPQGACAPLINDTISRVNSSMFASTCKPHELNNFIDLTLRNSFLISADMAHAIHPNYTA
NHEPLHRPALNKGPVIKYNANLRYASNGPTSFVILDICKKNGIPIQEFLVKNDSPCGSTI
GPIISGTYGIRTVDIGNPQLSMHSIRETCGVADITQVXXIQKYXNNYK

Frame C:
VXIFFKLPVQTSLVSKXRSIQFNYSTKSNIRNMEQYLPQAKEFISFIDKSPSPYHAVQYF
SEILKSKGFIHLSEKQMWDIQPNKKYFFTRNQSCISAFAVGGKYKPGNGFNIAAAHTDSP
NFKVRPVSKVESVGYQQVGVETYGGGLWYTWFDRDLTVAGRVIVKSGDGSYESKLVHIKK
PILRIPSLAIHLD---

---qlqsvvhlmnsfshhvviiwvchiaq*lvy*msknqhwhkrrmy*m*yysimkrlaq
vhhkvhvhh*smiplvvsiaqclhplvnhmn*tisli*l*eivs*slliwhmlfiqitqq
imnhfidqllikvq*snimqiyvmlqmvqlvllswisvkrmvsqfknssskmihhvvqql
vqsflvlmvlel*ilvihnyqciqfvklvvlxislryxxfknixtitk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL190 (AFL190Q) /CSM/AF/AFL1-D/AFL190Q.Seq.d/                       2397   0.0
CFG366 (CFG366Q) /CSM/CF/CFG3-C/CFG366Q.Seq.d/                       1320   0.0
SFC625 (SFC625Q) /CSM/SF/SFC6-B/SFC625Q.Seq.d/                       1292   0.0
AFD815 (AFD815Q) /CSM/AF/AFD8-A/AFD815Q.Seq.d/                       1292   0.0
AFB676 (AFB676Q) /CSM/AF/AFB6-D/AFB676Q.Seq.d/                       1292   0.0
SFL484 (SFL484Q) /CSM/SF/SFL4-D/SFL484Q.Seq.d/                       1291   0.0
CHA114 (CHA114Q) /CSM/CH/CHA1-A/CHA114Q.Seq.d/                       1291   0.0
AFM702 (AFM702Q) /CSM/AF/AFM7-A/AFM702Q.Seq.d/                       1291   0.0
AFK435 (AFK435Q) /CSM/AF/AFK4-B/AFK435Q.Seq.d/                       1291   0.0
VFI820 (VFI820Q) /CSM/VF/VFI8-A/VFI820Q.Seq.d/                       1289   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY133535|AY133535.1 Arabidopsis thaliana At5g60160/f15l12_20 mRNA, complete cds.    64   1e-12  3
AY085075|AY085075.1 Arabidopsis thaliana clone 126533 mRNA, complete sequence.    64   1e-12  3
AY045584|AY045584.1 Arabidopsis thaliana AT5g60160/f15l12_20 mRNA, complete cds.    64   1e-12  3
BG525546|BG525546.1 51-5 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    80   1e-10  1
AB026632|AB026632.1 Arabidopsis thaliana genomic DNA, chromosome 5, BAC clone:F15L12.    64   1e-09  5
CB259770|CB259770.1 23-E011176-013-009-M05-T7R MPIZ-ADIS-013 Arabidopsis thaliana cDNA clone MPIZp770M059Q 5-PRIME, mRNA sequence.    64   4e-07  2
AV823138|AV823138.1 Arabidopsis thaliana cDNA clone:RAFL05-15-M22, 5'-end.    64   6e-07  2
CA523816|CA523816.1 KS12029A03 KS12 Capsicum annuum cDNA, mRNA sequence.    66   1e-06  1
BU007153|BU007153.1 QGH13J06.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGH13J06, mRNA sequence.    50   2e-06  2
CA158596|CA158596.1 SCEZRZ3088A05.g RZ3 Saccharum officinarum cDNA clone SCEZRZ3088A05 5', mRNA sequence.    64   6e-06  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54M70) RecName: Full=Aspartyl aminopeptidase;          EC=3.4....   439   e-121
AC159446_25(AC159446|pid:none) Trypanosoma brucei chromosome 3 c...   224   5e-57
AM494966_233(AM494966|pid:none) Leishmania braziliensis chromoso...   217   8e-55
AJ720587_1(AJ720587|pid:none) Gallus gallus mRNA for hypothetica...   214   4e-54
CP000592_232(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   213   8e-54
CR954212_224(CR954212|pid:none) Ostreococcus tauri strain OTTH05...   212   2e-53
EU954490_1(EU954490|pid:none) Zea mays clone 1468685 unknown mRNA.    211   3e-53
BT062007_1(BT062007|pid:none) Zea mays full-length cDNA clone ZM...   211   3e-53
AP007151_1241(AP007151|pid:none) Aspergillus oryzae RIB40 genomi...   211   4e-53
AB008271_3(AB008271|pid:none) Arabidopsis thaliana genomic DNA, ...   211   5e-53

 psg: 0.84  gvh: 0.50  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFL190 is nuc

>AFL190F.Seq
ATGTTNAAATTTTTTTTAAATTACCCGTCCAAACTTCATTAGTGTCAAAATNAAGATCAA
TTCAATTTAATTATTCAACAAAAAGTAATATTAGAAATATGGAACAATACTTACCACAAG
CTAAGGAATTTATTTCATTTATTGATAAATCACCATCACCATATCATGCAGTTCAATACT
TTTCAGAGATTTTAAAGAGTAAAGGTTTCATTCATTTATCAGAGAAACAAATGTGGGATA
TTCAACCAAATAAAAAATATTTCTTTACTCGTAATCAATCATGTATTTCTGCTTTTGCAG
TTGGTGGTAAATATAAACCAGGTAATGGTTTTAATATTGCAGCAGCACATACTGATAGTC
CAAATTTTAAAGTTAGACCAGTATCAAAAGTTGAATCAGTTGGATATCAACAAGTTGGTG
TTGAAACATATGGTGGTGGATTATGGTATACATGGTTTGATCGTGATTTAACCGTTGCAG
GTAGAGTCATTGTTAAATCAGGTGATGGTAGTTATGAGAGTAAATTAGTTCACATAAAGA
AACCAATTCTTAGAATTCCATCATTAGCAATTCATCTCGATCG----------

>AFL190Z.Seq
----------ACCAGCTGCAATCGGTGGTGCACTTGATGAATTCATTTTCTCACCACGTT
GTGATAATTTGGGTATGTCATATTGCGCAATGATTGGTTTATTGAATGTCAAAGAATCAA
CATTGGCACAAGAGGAGAATGTATTGAATGTGATATTATTCGATAATGAAGAGGTTGGCT
CAAGTTCACCACAAGGTGCATGTGCACCATTGATCAATGATACCATTAGTCGTGTCAATA
GCTCAATGTTTGCATCCACTTGTAAACCACATGAATTAAACAATTTCATTGATTTAACTT
TAAGAAATAGTTTCTTAATCTCTGCTGATATGGCACATGCTATTCATCCAAATTACACAG
CAAATCATGAACCACTTCATAGACCAGCTCTTAATAAAGGTCCAGTAATCAAATATAATG
CAAATTTACGTTATGCTTCAAATGGTCCAACTAGTTTTGTTATCTTGGATATCTGTAAAA
AGAATGGTATCCCAATTCAAGAATTCCTCGTCAAAAATGATTCACCATGTGGTTCAACAA
TTGGTCCAATCATTTCTGGTACTTATGGTATTAGAACTGTAGATATTGGTAATCCACAAT
TATCAATGCATTCAATTCGTGAAACTTGTGGTGTTGCNGATATCACTCAGGTATNATTNA
TTCAAAAATATTTNAACAATTACAAAAT

>AFL190P.Seq
ATGTTNAAATTTTTTTTAAATTACCCGTCCAAACTTCATTAGTGTCAAAATNAAGATCAA
TTCAATTTAATTATTCAACAAAAAGTAATATTAGAAATATGGAACAATACTTACCACAAG
CTAAGGAATTTATTTCATTTATTGATAAATCACCATCACCATATCATGCAGTTCAATACT
TTTCAGAGATTTTAAAGAGTAAAGGTTTCATTCATTTATCAGAGAAACAAATGTGGGATA
TTCAACCAAATAAAAAATATTTCTTTACTCGTAATCAATCATGTATTTCTGCTTTTGCAG
TTGGTGGTAAATATAAACCAGGTAATGGTTTTAATATTGCAGCAGCACATACTGATAGTC
CAAATTTTAAAGTTAGACCAGTATCAAAAGTTGAATCAGTTGGATATCAACAAGTTGGTG
TTGAAACATATGGTGGTGGATTATGGTATACATGGTTTGATCGTGATTTAACCGTTGCAG
GTAGAGTCATTGTTAAATCAGGTGATGGTAGTTATGAGAGTAAATTAGTTCACATAAAGA
AACCAATTCTTAGAATTCCATCATTAGCAATTCATCTCGATCG----------ACCAGCT
GCAATCGGTGGTGCACTTGATGAATTCATTTTCTCACCACGTTGTGATAATTTGGGTATG
TCATATTGCGCAATGATTGGTTTATTGAATGTCAAAGAATCAACATTGGCACAAGAGGAG
AATGTATTGAATGTGATATTATTCGATAATGAAGAGGTTGGCTCAAGTTCACCACAAGGT
GCATGTGCACCATTGATCAATGATACCATTAGTCGTGTCAATAGCTCAATGTTTGCATCC
ACTTGTAAACCACATGAATTAAACAATTTCATTGATTTAACTTTAAGAAATAGTTTCTTA
ATCTCTGCTGATATGGCACATGCTATTCATCCAAATTACACAGCAAATCATGAACCACTT
CATAGACCAGCTCTTAATAAAGGTCCAGTAATCAAATATAATGCAAATTTACGTTATGCT
TCAAATGGTCCAACTAGTTTTGTTATCTTGGATATCTGTAAAAAGAATGGTATCCCAATT
CAAGAATTCCTCGTCAAAAATGATTCACCATGTGGTTCAACAATTGGTCCAATCATTTCT
GGTACTTATGGTATTAGAACTGTAGATATTGGTAATCCACAATTATCAATGCATTCAATT
CGTGAAACTTGTGGTGTTGCNGATATCACTCAGGTATNATTNATTCAAAAATATTTNAAC
AATTACAAAAT