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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL177
Library AF
(Link to library)
Clone ID AFL177
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16255-1
Original site URL
Representative seq. ID AFL177P
(Link to Original site)
Representative DNA sequence 
>AFL177 (AFL177Q) /CSM/AF/AFL1-D/AFL177Q.Seq.d/
CCTCAAGGTAATATTTATGAATTTTGGAGTAGTTGTACACCAAGTAGTGGTTTCACAGAT
TTTATTCCATCCAATGCAACATGTTCATCATTAAATTGTAATGCTCAACAAATGAGTTGC
AAATATGTTCAACAAGCATGTCATGAAACATCATGTTGTCCTGATATTCCTCAATGTCAA
ATTCCTGCAACTGGCGGTGGCCCAGCTACTGGAAGTGCAACTGGTCAAGGCACATCAGGT
GGTACACCAGGTTCTTGTGATAAAGTTAATTGTCCAAATGGTTACATTTGTACAATAGTA
AATCAATTAGCAGTATGTGTTTCTCCATCATCCTCTTCGTCAAGTTCTTCATCAACCACT
GGCTCACATACTACCACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCA
ACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGT
GGTTCAACCACTGGCTCACATACTACTACTGGNGGTTCAACTACTGGXXXXXXXXXXATG
TGTAAAGATTGAAAAACCAAAATGTCTCACTTGTAAAGATATGCATTGTGAGTCAAATGG
ATTACTCTGTGTCTTGACTCCACAAAAGAAGACTGATGAAGAATGTTGTCCAATTATTCC
AATCTGTATCAATCCATCAACTATTGCCGCATCCACTATTGCCACTACAACTGCATCAAC
TCGTCACTCAACTGCCTCAACAATTGCCTCATTAGTCACTGGTACCACAAGTGGTGGTGG
TGGTATGGGTTCATTTGGTGGTCGATCCGATGAAGAATCAAGTGATCCAAATGCTATACT
TGGTCTCTTTGAAGACGATATTTTCTGGGGTGATAATGATGAATACTTTAGTGATAACTA
CCGTTATGTTGATGAAGAAGATATTGATGAAGATTTCAATGGTAATGATGAATTTGAAAT
TGATGTTGAAGGTGATTTCCAAGAAGATGATTTCGAAGAAGAATATGCATTTTATTAAAT
TAAACTTTTTTTCATAAAAAAATAATAATAATATTAAAACAATCATATCATCAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
PQGNIYEFWSSCTPSSGFTDFIPSNATCSSLNCNAQQMSCKYVQQACHETSCCPDIPQCQ
IPATGGGPATGSATGQGTSGGTPGSCDKVNCPNGYICTIVNQLAVCVSPSSSSSSSSSTT
GSHTTTGGSTTGSHTTTGGSTTGSHTTTGGSTTGSHTTTGGSTTGSHTTTGGSTT---

---CVKIEKPKCLTCKDMHCESNGLLCVLTPQKKTDEECCPIIPICINPSTIAASTIATT
TASTRHSTASTIASLVTGTTSGGGGMGSFGGRSDEESSDPNAILGLFEDDIFWGDNDEYF
SDNYRYVDEEDIDEDFNGNDEFEIDVEGDFQEDDFEEEYAFY*iklffikk***y*nnhi
i
Translated Amino Acid sequence (All Frames) 
Frame A:
PQGNIYEFWSSCTPSSGFTDFIPSNATCSSLNCNAQQMSCKYVQQACHETSCCPDIPQCQ
IPATGGGPATGSATGQGTSGGTPGSCDKVNCPNGYICTIVNQLAVCVSPSSSSSSSSSTT
GSHTTTGGSTTGSHTTTGGSTTGSHTTTGGSTTGSHTTTGGSTTGSHTTTGGSTT---

---mckd*ktkmshl*ryal*vkwitlcldstked**rmlsnysnlyqsinycrihychy
ncinsslnclnnclishwyhkwwwwygfiwwsir*rik*skcytwsl*rryflg****il
***lplc**rry**rfqw***i*n*c*r*fprr*frrricilln*tffhkkiiiilkqsy
hq

Frame B:
lkvifmnfgvvvhqvvvsqilfhpmqhvhh*ivmlnk*vanmfnkhvmkhhvvliflnvk
flqlavaqllevqlvkahqvvhqvlviklivqmvtfvq**in*qyvflhhplrqvlhqpl
ahilplvvqplahilllvvqplahilllvvqplahilllvvqplahilllxvqll---

---CVKIEKPKCLTCKDMHCESNGLLCVLTPQKKTDEECCPIIPICINPSTIAASTIATT
TASTRHSTASTIASLVTGTTSGGGGMGSFGGRSDEESSDPNAILGLFEDDIFWGDNDEYF
SDNYRYVDEEDIDEDFNGNDEFEIDVEGDFQEDDFEEEYAFY*iklffikk***y*nnhi
i

Frame C:
sr*yl*ile*lytk*wfhrfysiqcnmfiikl*cstnelqicstsms*nimls*yssmsn
scnwrwpsywkcnwsrhirwytrfl**s*lskwlhlynsksissmcfsiilfvkffinhw
ltyyhwwfnhwltyyywwfnhwltyyywwfnhwltyyywwfnhwltyyywxfnyw---

---v*rlknqnvslvkicivsqmdysvs*lhkrrlmknvvqlfqsvsihqllphpllplq
lhqlvtqlpqqlph*slvpqvvvvvwvhlvvdpmknqviqmlylvslktifsgvimmntl
vittvmlmkkilmkismvmmnlklmlkviskkmiskknmhfiklnffs*knnnniktiis
s
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL177 (AFL177Q) /CSM/AF/AFL1-D/AFL177Q.Seq.d/                       1980   0.0
SFD793 (SFD793Q) /CSM/SF/SFD7-D/SFD793Q.Seq.d/                       1039   0.0
CHD579 (CHD579Q) /CSM/CH/CHD5-D/CHD579Q.Seq.d/                       1039   0.0
CHC435 (CHC435Q) /CSM/CH/CHC4-B/CHC435Q.Seq.d/                       1039   0.0
CFI766 (CFI766Q) /CSM/CF/CFI7-C/CFI766Q.Seq.d/                       1039   0.0
CFH746 (CFH746Q) /CSM/CF/CFH7-B/CFH746Q.Seq.d/                       1039   0.0
CFF856 (CFF856Q) /CSM/CF/CFF8-C/CFF856Q.Seq.d/                       1039   0.0
AFN509 (AFN509Q) /CSM/AF/AFN5-A/AFN509Q.Seq.d/                       1039   0.0
CHA516 (CHA516Q) /CSM/CH/CHA5-A/CHA516Q.Seq.d/                       1037   0.0
SLE232 (SLE232Q) /CSM/SL/SLE2-B/SLE232Q.Seq.d/                       1031   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26238|M26238.1 D.discoideum spore coat protein SP70 gene, complete cds.  1039   0.0    2
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.  1023   0.0    8
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    40   2e-07  14
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   2e-06  16
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    34   4e-06  13
X16491|X16491.1 Dictyostelium discoideum sp96 gene for spore coat protein SP96.    34   6e-06  3
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   8e-06  17
AC136724|AC136724.5 Mus musculus chromosome 16 clone RP23-65F24 map 16, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    28   1e-05  4
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    34   9e-04  14
AC005140|AC005140.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    50   0.001  9
dna update 2004. 8. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   184   4e-45
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...   131   5e-36
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        74   1e-11
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    74   1e-11
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    57   1e-06
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    45   0.005
DQ059011_2(DQ059011|pid:none) Samia cynthia ricini NADH dehydrog...    42   0.035
CP000478_3884(CP000478|pid:none) Syntrophobacter fumaroxidans MP...    42   0.046
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    41   0.060
BA000030_4488(BA000030|pid:none) Streptomyces avermitilis MA-468...    40   0.17
protein update 2009. 4.25
PSORT 

 psg: 0.75  gvh: 0.47  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFL177 is cyt
5' end seq. ID AFL177F
5' end seq. 
>AFL177F.Seq
CCTCAAGGTAATATTTATGAATTTTGGAGTAGTTGTACACCAAGTAGTGGTTTCACAGAT
TTTATTCCATCCAATGCAACATGTTCATCATTAAATTGTAATGCTCAACAAATGAGTTGC
AAATATGTTCAACAAGCATGTCATGAAACATCATGTTGTCCTGATATTCCTCAATGTCAA
ATTCCTGCAACTGGCGGTGGCCCAGCTACTGGAAGTGCAACTGGTCAAGGCACATCAGGT
GGTACACCAGGTTCTTGTGATAAAGTTAATTGTCCAAATGGTTACATTTGTACAATAGTA
AATCAATTAGCAGTATGTGTTTCTCCATCATCCTCTTCGTCAAGTTCTTCATCAACCACT
GGCTCACATACTACCACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCA
ACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGT
GGTTCAACCACTGGCTCACATACTACTACTGGNGGTTCAACTACTGG----------
Length of 5' end seq. 527
3' end seq. ID AFL177Z
3' end seq. 
>AFL177Z.Seq
----------ATGTGTAAAGATTGAAAAACCAAAATGTCTCACTTGTAAAGATATGCATT
GTGAGTCAAATGGATTACTCTGTGTCTTGACTCCACAAAAGAAGACTGATGAAGAATGTT
GTCCAATTATTCCAATCTGTATCAATCCATCAACTATTGCCGCATCCACTATTGCCACTA
CAACTGCATCAACTCGTCACTCAACTGCCTCAACAATTGCCTCATTAGTCACTGGTACCA
CAAGTGGTGGTGGTGGTATGGGTTCATTTGGTGGTCGATCCGATGAAGAATCAAGTGATC
CAAATGCTATACTTGGTCTCTTTGAAGACGATATTTTCTGGGGTGATAATGATGAATACT
TTAGTGATAACTACCGTTATGTTGATGAAGAAGATATTGATGAAGATTTCAATGGTAATG
ATGAATTTGAAATTGATGTTGAAGGTGATTTCCAAGAAGATGATTTCGAAGAAGAATATG
CATTTTATTAAATTAAACTTTTTTTCATAAAAAAATAATAATAATATTAAAACAATCATA
TCATCAA
Length of 3' end seq. 537
Connected seq. ID AFL177P
Connected seq. 
>AFL177P.Seq
CCTCAAGGTAATATTTATGAATTTTGGAGTAGTTGTACACCAAGTAGTGGTTTCACAGAT
TTTATTCCATCCAATGCAACATGTTCATCATTAAATTGTAATGCTCAACAAATGAGTTGC
AAATATGTTCAACAAGCATGTCATGAAACATCATGTTGTCCTGATATTCCTCAATGTCAA
ATTCCTGCAACTGGCGGTGGCCCAGCTACTGGAAGTGCAACTGGTCAAGGCACATCAGGT
GGTACACCAGGTTCTTGTGATAAAGTTAATTGTCCAAATGGTTACATTTGTACAATAGTA
AATCAATTAGCAGTATGTGTTTCTCCATCATCCTCTTCGTCAAGTTCTTCATCAACCACT
GGCTCACATACTACCACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCA
ACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGT
GGTTCAACCACTGGCTCACATACTACTACTGGNGGTTCAACTACTGG----------ATG
TGTAAAGATTGAAAAACCAAAATGTCTCACTTGTAAAGATATGCATTGTGAGTCAAATGG
ATTACTCTGTGTCTTGACTCCACAAAAGAAGACTGATGAAGAATGTTGTCCAATTATTCC
AATCTGTATCAATCCATCAACTATTGCCGCATCCACTATTGCCACTACAACTGCATCAAC
TCGTCACTCAACTGCCTCAACAATTGCCTCATTAGTCACTGGTACCACAAGTGGTGGTGG
TGGTATGGGTTCATTTGGTGGTCGATCCGATGAAGAATCAAGTGATCCAAATGCTATACT
TGGTCTCTTTGAAGACGATATTTTCTGGGGTGATAATGATGAATACTTTAGTGATAACTA
CCGTTATGTTGATGAAGAAGATATTGATGAAGATTTCAATGGTAATGATGAATTTGAAAT
TGATGTTGAAGGTGATTTCCAAGAAGATGATTTCGAAGAAGAATATGCATTTTATTAAAT
TAAACTTTTTTTCATAAAAAAATAATAATAATATTAAAACAATCATATCATCAA
Length of connected seq. 1064
Full length Seq ID -
Full length Seq. -
Length of full length seq. -