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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL176
Library AF
(Link to library)
Clone ID AFL176
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U02881-1
Original site URL
Representative seq. ID AFL176Z
(Link to Original site)
Representative DNA sequence 
>AFL176 (AFL176Q) /CSM/AF/AFL1-D/AFL176Q.Seq.d/
XXXXXXXXXXAAACAACAACAAGTCAATGATGAAGATTGTTCATCAAACAAACAAAGTAA
TTCATTTTCTGGCAATGTCCTAGCCTTTGGCAATATGATTTTAAAAGGTCTTTCATTTTC
TGATTTAACAGGAAATAATAATAATAATAATATTAATAATAATAATAATAATAATAATAA
AAATAATNCCAACCCTAATTATACCAATAATGTTGTAGTTAATGATAATATTTGTGAAAA
AGACAAAGAAAATACNCAAGATCAACAAACAAATACAGTTACAGTTTCAACTACTATTAT
CAATACAACCACAACTTCAACCACTTCTGAAAATAGTAATACAATTGATAAAAGTAATGA
TAGTATAAATGAAAGTGGTATTAGCTCATTGGATGAATCAAACAATTCAACTAGTGGCGA
TTTAATTTCTTCAAATTCTTCCTCTTTCAGTTCAATGGANGATATATAATCAACTTTTAA
TAATTATTCNCAACNACNGCTCCTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---QQQVNDEDCSSNKQSNSFSGNVLAFGNMILKGLSFSDLTGNNNNNNINNNNNNNNKN
NXNPNYTNNVVVNDNICEKDKENTQDQQTNTVTVSTTIINTTTTSTTSENSNTIDKSNDS
INESGISSLDESNNSTSGDLISSNSSSFSSMXDI*stfnnysqxxll
Translated Amino Acid sequence (All Frames) 
Frame A:
---nnnksmmkivhqtnkvihflams*plai*f*kvfhfli*qeiiiiiiliiiiiiiik
ixptliipiml*lmiifvkktkkixkinkqiqlqfqlllsiqpqlqpllkiviqlikvmi
v*mkvvlahwmnqtiqlvai*flqilplsvqwxiynqlliiixnxxss


Frame B:
---tttsq**rlfikqtk*fifwqcpslwqydfkrsfif*fnrk*****y*********k
*xqp*lyq*ccs***yl*krqrkyxrstnkysysfnyyyqynhnfnhf*k**yn**k***
yk*kwy*lig*ikqfn*wrfnffkfflfqfngxyiinf**lfxttap


Frame C:
---QQQVNDEDCSSNKQSNSFSGNVLAFGNMILKGLSFSDLTGNNNNNNINNNNNNNNKN
NXNPNYTNNVVVNDNICEKDKENTQDQQTNTVTVSTTIINTTTTSTTSENSNTIDKSNDS
INESGISSLDESNNSTSGDLISSNSSSFSSMXDI*stfnnysqxxll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL176 (AFL176Q) /CSM/AF/AFL1-D/AFL176Q.Seq.d/                        601   e-171
AFJ823 (AFJ823Q) /CSM/AF/AFJ8-A/AFJ823Q.Seq.d/                        565   e-160
SFJ166 (SFJ166Q) /CSM/SF/SFJ1-C/SFJ166Q.Seq.d/                        523   e-148
SLI342 (SLI342Q) /CSM/SL/SLI3-B/SLI342Q.Seq.d/                         44   0.002
SLB332 (SLB332Q) /CSM/SL/SLB3-B/SLB332Q.Seq.d/                         44   0.002
CHR851 (CHR851Q) /CSM/CH/CHR8-C/CHR851Q.Seq.d/                         42   0.008
CHN112 (CHN112Q) /CSM/CH/CHN1-A/CHN112Q.Seq.d/                         42   0.008
SSH542 (SSH542Q) /CSM/SS/SSH5-B/SSH542Q.Seq.d/                         40   0.032
SSD121 (SSD121Q) /CSM/SS/SSD1-A/SSD121Q.Seq.d/                         40   0.032
SLG252 (SLG252Q) /CSM/SL/SLG2-C/SLG252Q.Seq.d/                         38   0.13
own update 2002.10.30
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    46   2e-05  9
AC104563|AC104563.14 Homo sapiens chromosome 11, clone RP11-587D21, complete sequence.    44   0.010  2
AP004826|AP004826.1 Staphylococcus aureus subsp. aureus MW2 DNA, complete genome, strain:MW2, section 5/10.    38   0.018  9
AP003133|AP003133.2 Staphylococcus aureus subsp. aureus N315 genomic DNA, complete genome, section 5/10.    38   0.021  9
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            34   0.023  18
AL954768|AL954768.7 Zebrafish DNA sequence from clone CH211-254L23 in linkage group 12.    34   0.026  6
AC112402|AC112402.8 Rattus norvegicus clone CH230-182J19, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    36   0.048  8
AC073387|AC073387.4 Homo sapiens BAC clone RP11-10E6 from 7, complete sequence.    34   0.063  7
AL845319|AL845319.10 Mouse DNA sequence from clone RP23-429I7 on chromosome 2.    38   0.064  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38   0.079  12
dna update 2004. 8. 8
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 304,865,495
protein update 2009. 4.25
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for AFL176 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFL176Z
3' end seq. 
>AFL176Z.Seq
----------AAACAACAACAAGTCAATGATGAAGATTGTTCATCAAACAAACAAAGTAA
TTCATTTTCTGGCAATGTCCTAGCCTTTGGCAATATGATTTTAAAAGGTCTTTCATTTTC
TGATTTAACAGGAAATAATAATAATAATAATATTAATAATAATAATAATAATAATAATAA
AAATAATNCCAACCCTAATTATACCAATAATGTTGTAGTTAATGATAATATTTGTGAAAA
AGACAAAGAAAATACNCAAGATCAACAAACAAATACAGTTACAGTTTCAACTACTATTAT
CAATACAACCACAACTTCAACCACTTCTGAAAATAGTAATACAATTGATAAAAGTAATGA
TAGTATAAATGAAAGTGGTATTAGCTCATTGGATGAATCAAACAATTCAACTAGTGGCGA
TTTAATTTCTTCAAATTCTTCCTCTTTCAGTTCAATGGANGATATATAATCAACTTTTAA
TAATTATTCNCAACNACNGCTCCTCA
Length of 3' end seq. 496
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -