>AFB334 (AFB334Q) /CSM/AF/AFB3-B/AFB334Q.Seq.d/
CACTGTTGGCCTACTGGATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATT
ATATATATATAAAAATATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTA
TTTTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAA
AGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTA
TCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGAT
GATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGT
AAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTA
GGTTGTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACT
TCTTGTTGCCCAAGAATTCCAAGATGTGTTGGTCATCATGGTGGCGGNCATAAATGTGAC
AGAATGAGATGTCXXXXXXXXXXTTTGTATTCATCCATCAACTACTGATGCTTCAACCAT
TGCAACAANTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAAC
TGGTGGAACTACAACTGGTGGNTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAG
TAGCGCTGCCCCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACC
ATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTNCAAGTAGCGCTCCATCCTC
TGCCTCAAGTAGTGCTCCANTCATTCATCAGCT

hcwptgfiqfcnykkrnqiiiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCE
SHRSEYTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSC
KPHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCD
RMRC---

---FVFIHQLLMLQPLQQXHQLSQLQPLQLLQVQQLVELQLVXQLLIVVLLPQPIVALPP
HHHQAVLLQVPLQVNHQVAPLQVVLHPLLQVALHPLPQVVLXSFIS

Frame A:
hcwptgfiqfcnykkrnqiiiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCE
SHRSEYTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSC
KPHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCD
RMRC---

---FVFIHQLLMLQPLQQXHQLSQLQPLQLLQVQQLVELQLVXQLLIVVLLPQPIVALPP
HHHQAVLLQVPLQVNHQVAPLQVVLHPLLQVALHPLPQVVLXSFIS

Frame B:
tvglldsfnfviikkgik*lyiyknikkik*efyi*llfyf*lhyiyhqllvgvvaeivk
vivqnihvnqievvhiyhlyhvvvrknfsvliviieivvmiyhvlktrelvkslrfgqvv
nhieilyhiihqiqphvnh*vvkqevwnvnglnhhhvmellvaqefqdvlvimvaxinvt
e*dv---

---lyssiny*cfnhcnxcincrnynlcnycrynnwwnynwwxnf***ccflsr**rcpl
itikqccfkcrfk*tik*rrfk*csilcxk*rsilclk*csxhssa

Frame C:
llaywihsil*l*kkesnnyiyiki*kk*nenfifsfsfifnyiisitnfwlgwwqrl*k
ssfriym*ir*klcifticimlw*eriflc*s*s*kll**fimc*khenw*nl*dlvkl*
tt*rfctisftkynhm*iirl*skrygm*mg*iitmlwnfllpknskmcwsswwrx*m*q
nem---

---cihpsttdastiatxastvatttsattagtttggtttggstsdssaassadssaaps
spsssaassaassepsssaasssapssaxssapssasssapxihq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG851 (SLG851Q) /CSM/SL/SLG8-C/SLG851Q.Seq.d/                        910   0.0
SLB538 (SLB538Q) /CSM/SL/SLB5-B/SLB538Q.Seq.d/                        910   0.0
SLA438 (SLA438Q) /CSM/SL/SLA4-B/SLA438Q.Seq.d/                        910   0.0
SLA432 (SLA432Q) /CSM/SL/SLA4-B/SLA432Q.Seq.d/                        910   0.0
SLA402 (SLA402Q) /CSM/SL/SLA4-A/SLA402Q.Seq.d/                        910   0.0
SHJ527 (SHJ527Q) /CSM/SH/SHJ5-B/SHJ527Q.Seq.d/                        910   0.0
SHH249 (SHH249Q) /CSM/SH/SHH2-C/SHH249Q.Seq.d/                        910   0.0
SHF542 (SHF542Q) /CSM/SH/SHF5-B/SHF542Q.Seq.d/                        910   0.0
SHD608 (SHD608Q) /CSM/SH/SHD6-A/SHD608Q.Seq.d/                        910   0.0
SHC423 (SHC423Q) /CSM/SH/SHC4-A/SHC423Q.Seq.d/                        910   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.   745  0.0  4
AZ208391|AZ208391.1 SP_0150_A1_B06_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=150 Col=11 Row=C, DNA sequence.    48  0.18  1
BM755316|BM755316.1 K-EST0033042 S10SNU1 Homo sapiens cDNA clone S10SNU1-7-D07 5', mRNA sequence.    48  0.18  1
BX120001|BX120001.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-217J8.    46  0.72  1
BD101381|BD101381.1 Novel genes cloned in humanneuroblastoma and fragments thereof.    46  0.72  1
BD021443|BD021443.1 Novel gene and novel gene fragment cloned in human neuroblastoma.    46  0.72  1
BX119921|BX119921.8 Zebrafish DNA sequence from clone CH211-198N10 in linkage group 16.    46  0.72  1
BX519876|BX519876.1 RZPD Mus musculus cDNA clone IMAGp998C195950 = IMAGE:2395338 5' EST.    46  0.72  1
BD101699|BD101699.1 Novel genes cloned in humanneuroblastoma and fragments thereof.    40  0.98  2
BD097381|BD097381.1 Nucleic acid sequences characterized by their enhanced expression in good prognostic human neuroblastoma upon comparison between good prognostic human neuroblastoma and poor prognostic human neuroblastoma.    40  0.98  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       353   5e-96
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   349   6e-95
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   131   2e-29
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    97   7e-19
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    64   8e-09
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    62   2e-08
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    53   1e-05
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    48   4e-04
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    47   6e-04
FJ501956_1(FJ501956|pid:none) Oryza sativa Indica Group keratin ...    40   0.096

 psg: 0.96  gvh: 0.77  alm: 0.27  top: 0.10  tms: 0.07  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.20  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	  4.0 %: nuclear

>> prediction for AFB334 is exc

>AFB334F.Seq
CACTGTTGGCCTACTGGATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATT
ATATATATATAAAAATATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTA
TTTTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAA
AGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTA
TCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGAT
GATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGT
AAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTA
GGTTGTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACT
TCTTGTTGCCCAAGAATTCCAAGATGTGTTGGTCATCATGGTGGCGGNCATAAATGTGAC
AGAATGAGATGTC----------

>AFB334Z.Seq
----------TTTGTATTCATCCATCAACTACTGATGCTTCAACCATTGCAACAANTGCA
TCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAACTACA
ACTGGTGGNTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCCCC
TCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGCC
GCTTCAAGTAGTGCTCCATCCTCTGCTNCAAGTAGCGCTCCATCCTCTGCCTCAAGTAGT
GCTCCANTCATTCATCAGCT

>AFB334P.Seq
CACTGTTGGCCTACTGGATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATT
ATATATATATAAAAATATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTA
TTTTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAA
AGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTA
TCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGAT
GATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGT
AAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTA
GGTTGTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACT
TCTTGTTGCCCAAGAATTCCAAGATGTGTTGGTCATCATGGTGGCGGNCATAAATGTGAC
AGAATGAGATGTC----------TTTGTATTCATCCATCAACTACTGATGCTTCAACCAT
TGCAACAANTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAAC
TGGTGGAACTACAACTGGTGGNTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAG
TAGCGCTGCCCCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACC
ATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTNCAAGTAGCGCTCCATCCTC
TGCCTCAAGTAGTGCTCCANTCATTCATCAGCT