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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL143
Library AF
(Link to library)
Clone ID AFL143
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFL143P
(Link to Original site)
Representative DNA sequence 
>AFL143 (AFL143Q) /CSM/AF/AFL1-B/AFL143Q.Seq.d/
AAACAATTNTCAGCCANCAAAAANATTGCAATTGCAAAAGTTGATTGTNCTCAACATGAA
CAACTNTGTAAACAAAATAAAGTTCANGGTTNTCCAACTTTAGTTGTTTTCAANAANGGT
AAAGCTXXXXXXXXXXTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGT
TGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTT
GTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAA
GAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTT
TCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCC
GTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTC
TACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAA
TACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTA
CCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAA
GAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATT
CAAGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGANATAATGTNAATCT
AAAAAAGATTCTTCTGCTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KQXSAXKXIAIAKVDCXQHEQLCKQNKVXGXPTLVVFXXGKA---

---KKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETF
SFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAP
WCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENP
ISYEGQRNDHMNFVEFIQDNAAXNSNYHHLKLX*cxskkdssa
Translated Amino Acid sequence (All Frames) 
Frame A:
KQXSAXKXIAIAKVDCXQHEQLCKQNKVXGXPTLVVFXXGKA---

---*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkkp
shsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstl
hgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkki
qslmkvkemii*tllnsfkitlpxiqitiisn*xnvnlkkilll

Frame B:
nnxqpxkxlqlqklivlnmnnxvnkikfxvxql*lfsxxvk---

---kenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnl
liryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrs
mvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrks
nll*rskk*syelc*ihsr*rchxfklpssqtximxi*krffc*

Frame C:
tixsxqkxcncks*lxst*ttx*tk*ssxxsnfscfqxx*s---

---KKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETF
SFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAP
WCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENP
ISYEGQRNDHMNFVEFIQDNAAXNSNYHHLKLX*cxskkdssa
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL143 (AFL143Q) /CSM/AF/AFL1-B/AFL143Q.Seq.d/                       1423   0.0
AFL139 (AFL139Q) /CSM/AF/AFL1-B/AFL139Q.Seq.d/                       1298   0.0
AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/                       1292   0.0
AFK858 (AFK858Q) /CSM/AF/AFK8-C/AFK858Q.Seq.d/                       1287   0.0
AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/                       1287   0.0
AFO433 (AFO433Q) /CSM/AF/AFO4-B/AFO433Q.Seq.d/                       1285   0.0
AFO247 (AFO247Q) /CSM/AF/AFO2-B/AFO247Q.Seq.d/                       1285   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1285   0.0
AFM548 (AFM548Q) /CSM/AF/AFM5-B/AFM548Q.Seq.d/                       1285   0.0
AFM212 (AFM212Q) /CSM/AF/AFM2-A/AFM212Q.Seq.d/                       1285   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   6e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   6e-08  1
CK029548|CK029548.1 AGENCOURT_16620510 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7051751 5', mRNA sequence.    70   6e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   6e-08  1
dna update 2004. 8. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   170   5e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   169   7e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   166   6e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   2e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   160   4e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   5e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   3e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   3e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   3e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   3e-35
protein update 2009. 4.25
PSORT 

 psg: 0.69  gvh: 0.39  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFL143 is nuc
5' end seq. ID AFL143F
5' end seq. 
>AFL143F.Seq
AAACAATTNTCAGCCANCAAAAANATTGCAATTGCAAAAGTTGATTGTNCTCAACATGAA
CAACTNTGTAAACAAAATAAAGTTCANGGTTNTCCAACTTTAGTTGTTTTCAANAANGGT
AAAGCT----------
Length of 5' end seq. 126
3' end seq. ID AFL143Z
3' end seq. 
>AFL143Z.Seq
----------TAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTC
GATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCA
GCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACC
TTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCA
TTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGT
ACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCT
CCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTA
AAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGT
GATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAAT
CCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGAT
AACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGANATAATGTNAATCTAAAAAA
GATTCTTCTGCTAA
Length of 3' end seq. 664
Connected seq. ID AFL143P
Connected seq. 
>AFL143P.Seq
AAACAATTNTCAGCCANCAAAAANATTGCAATTGCAAAAGTTGATTGTNCTCAACATGAA
CAACTNTGTAAACAAAATAAAGTTCANGGTTNTCCAACTTTAGTTGTTTTCAANAANGGT
AAAGCT----------TAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGT
TGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTT
GTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAA
GAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTT
TCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCC
GTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTC
TACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAA
TACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTA
CCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAA
GAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATT
CAAGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGANATAATGTNAATCT
AAAAAAGATTCTTCTGCTAA
Length of connected seq. 790
Full length Seq ID -
Full length Seq. -
Length of full length seq. -