>AFL139 (AFL139Q) /CSM/AF/AFL1-B/AFL139Q.Seq.d/
XXXXXXXXXXGATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTG
GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGT
TCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGA
AACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTC
TCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGT
TGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTA
CGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATA
CTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACC
AAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGA
AAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCA
AGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGAGATAATGTGAATCTAA
AAAAGATTCTTCTGCTAA

---VKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKET
FSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYA
PWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKEN
PISYEGQRNDHMNFVEFIQDNAAXNSNYHHLKLR*ceskkdssa

Frame A:
---m*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkk
pshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnst
lhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkk
iqslmkvkemii*tllnsfkitlpxiqitiisn*dnvnlkkilll


Frame B:
---ckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krn
lliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilr
smvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrk
snll*rskk*syelc*ihsr*rchxfklpssqteim*i*krffc*


Frame C:
---VKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKET
FSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYA
PWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKEN
PISYEGQRNDHMNFVEFIQDNAAXNSNYHHLKLR*ceskkdssa

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL139 (AFL139Q) /CSM/AF/AFL1-B/AFL139Q.Seq.d/                       1318   0.0
AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/                       1306   0.0
AFK858 (AFK858Q) /CSM/AF/AFK8-C/AFK858Q.Seq.d/                       1300   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1298   0.0
AFM548 (AFM548Q) /CSM/AF/AFM5-B/AFM548Q.Seq.d/                       1298   0.0
AFM212 (AFM212Q) /CSM/AF/AFM2-A/AFM212Q.Seq.d/                       1298   0.0
AFL143 (AFL143Q) /CSM/AF/AFL1-B/AFL143Q.Seq.d/                       1298   0.0
AFK539 (AFK539Q) /CSM/AF/AFK5-B/AFK539Q.Seq.d/                       1298   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1298   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1298   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   172   1e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   3e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   167   3e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   9e-40
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   160   2e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   3e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   2e-35

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.47  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFL139 is nuc

>AFL139Z.Seq
----------GATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTG
GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGT
TCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGA
AACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTC
TCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGT
TGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTA
CGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATA
CTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACC
AAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGA
AAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCA
AGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGAGATAATGTGAATCTAA
AAAAGATTCTTCTGCTAA