>AFB330 (AFB330Q) /CSM/AF/AFB3-B/AFB330Q.Seq.d/
XXXXXXXXXXCTCAACATAAACCATTATTACATGATATTTTAGATAGTATTTTAAAGGAA
CAAATTAAAAGAAACCTCTTATCCATACTTATCAACAACTCAATCAAGAGAGAGACCACA
AGATGTAATTATATTTATGGTTGGTGGTATCACTTATGAAGAGGCATTAACCGTTTACAC
TTTTAATTCTTTAAATACTGGTGTTTGTCGTGTTGTATTAGGTGGTACTTCAATTTTAAA
TCGTGAACAATTTTTAGAAGATTTATCAAGTACTCAAATTTCTAATCCTTCCTAGTAGTA
GTGGTAGAAGATAAAAATAAAAAAATAAAA

---ninhyymif*ivf*RNKLKETSYPYLSTTQSRERPQDVIIFMVGGITYEEALTVYTF
NSLNTGVCRVVLGGTSILNREQFLEDLSSTQISNPS***w*kikikk*

Frame A:
---st*tiit*yfr*yfkgtn*kkplihtyqqlnqerdhkm*lylwlvvslmkrh*pftl
lil*ilvfvvly*vvlqf*ivnnf*kiyqvlkflilpsssgrr*k*knk


Frame B:
---qhkpllhdildsilkeqikrnllsilinnsikrettrcnyiygwwyhl*rginrlhf
*ffkywclsccirwyfnfks*tifrrfikysnf*sflvvvvedknkkik


Frame C:
---ninhyymif*ivf*RNKLKETSYPYLSTTQSRERPQDVIIFMVGGITYEEALTVYTF
NSLNTGVCRVVLGGTSILNREQFLEDLSSTQISNPS***w*kikikk*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB330 (AFB330Q) /CSM/AF/AFB3-B/AFB330Q.Seq.d/                        599   e-170
VFK543 (VFK543Q) /CSM/VF/VFK5-B/VFK543Q.Seq.d/                        567   e-161
AFI471 (AFI471Q) /CSM/AF/AFI4-C/AFI471Q.Seq.d/                        567   e-161
SSJ808 (SSJ808Q) /CSM/SS/SSJ8-A/SSJ808Q.Seq.d/                        563   e-160
SSC757 (SSC757Q) /CSM/SS/SSC7-C/SSC757Q.Seq.d/                        561   e-159
FC-BS11 (FC-BS11Q) /CSM/FC/FC-BS/FC-BS11Q.Seq.d/                      559   e-159
AFI651 (AFI651Q) /CSM/AF/AFI6-C/AFI651Q.Seq.d/                        555   e-157
SSJ643 (SSJ643Q) /CSM/SS/SSJ6-B/SSJ643Q.Seq.d/                        553   e-157
SFC628 (SFC628Q) /CSM/SF/SFC6-B/SFC628Q.Seq.d/                        551   e-156
CHB225 (CHB225Q) /CSM/CH/CHB2-B/CHB225Q.Seq.d/                        551   e-156

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF303741|AF303741.1 Chilo iridescent virus complete genome.       34  0.078  7
AC016171|AC016171.4 Homo sapiens clone RP11-19E24, WORKING DRAFT SEQUENCE, 20 unordered pieces.    36  0.32  6
L13731|L13731.1 Onchocerca volvulus TATA box-binding protein gene, complete cds.    42  0.77  2
AC125639|AC125639.4 Rattus norvegicus clone CH230-10N10, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44  1.0  1
AL450490|AL450490.6 Human DNA sequence from clone RP11-124N4 on chromosome X.    44  1.0  1
AC132906|AC132906.3 Mus musculus clone RP24-297H12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  1.0  1
AF238325|AF238325.1 Dictyostelium discoideum lysosomal integral membrane protein II (lmpC) mRNA, complete cds.    44  1.0  1
AQ752519|AQ752519.1 HS_5572_B1_E03_SP6 RPCI-11 Human Male BAC Library Homo sapiens genomic clone Plate=1148 Col=5 Row=J, genomic survey sequence.    44  1.0  1
AC111849|AC111849.4 Rattus norvegicus clone CH230-275P12, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34  1.5  6
BZ250021|BZ250021.1 CH230-315E3.TV CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-315E3, DNA sequence.    36  1.9  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54GE3) RecName: Full=Vacuolar protein sorting-associated prote...   157   2e-42
BC123422_1(BC123422|pid:none) Bos taurus vacuolar protein sortin...    85   8e-16
AK146190_1(AK146190|pid:none) Mus musculus CRL-1722 L5178Y-R cDN...    84   1e-15
AK295529_1(AK295529|pid:none) Homo sapiens cDNA FLJ54353 complet...    84   1e-15
AK223214_1(AK223214|pid:none) Homo sapiens mRNA for vacuolar pro...    84   1e-15
AJ133421_1(AJ133421|pid:none) Homo sapiens mRNA for leucocyte va...    84   1e-15
(Q9NRW7) RecName: Full=Vacuolar protein sorting-associated prote...    84   1e-15
BC121938_1(BC121938|pid:none) Xenopus tropicalis vacuolar protei...    83   2e-15
BC084162_1(BC084162|pid:none) Xenopus laevis hypothetical LOC495...    82   5e-15
BT045798_1(BT045798|pid:none) Salmo salar clone ssal-rgf-531-252...    81   1e-14

 psg: 0.80  gvh: 0.41  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for AFB330 is nuc

>AFB330Z.Seq
----------CTCAACATAAACCATTATTACATGATATTTTAGATAGTATTTTAAAGGAA
CAAATTAAAAGAAACCTCTTATCCATACTTATCAACAACTCAATCAAGAGAGAGACCACA
AGATGTAATTATATTTATGGTTGGTGGTATCACTTATGAAGAGGCATTAACCGTTTACAC
TTTTAATTCTTTAAATACTGGTGTTTGTCGTGTTGTATTAGGTGGTACTTCAATTTTAAA
TCGTGAACAATTTTTAGAAGATTTATCAAGTACTCAAATTTCTAATCCTTCCTAGTAGTA
GTGGTAGAAGATAAAAATAAAAAAATAAAA