>AFL124 (AFL124Q) /CSM/AF/AFL1-A/AFL124Q.Seq.d/
CAAGATANTGAAGAAATTAAAATGAGATTACAANGTGCTCATCAACAATTACAAGAACAA
TCACCTGCANTCTATCAATCAATGAAATCTGAAGATCTAAGGAAATTTAGATGATGAAGT
AATTCGTAATACTCTTAAGGAAATGCAAATTCAACGTGAAAATCAAGATATTGGTCAACA
AAATCAAGAAGATAAAGAACAATTAATNGATTTACAAAATAGAGAACCTGGTTTATACAA
AAATCAACAAGATCTTAAACAAGAGAAAAGAGCCAATCAACAAGAATTAATCAATTATGA
ATTGAATTTACAAGAAGATCACAGAACANTATGAACTTTTATTAGATCNNCTCTATGATG
AACAACANCAACAACCACAGGAAAGTTTCAAATAAACAACANCTTCAAGAACAACAAATT
AATTCACCCGAGGATATTCAATATCAATTAAATCATTTAAATCAACCATTCCAAGATGAC
TATCATAATGATCAAACTGAAGAATTANNAGATGATGATTATAATTTTAATGATCAACAA
ATTAACAATGGTCAATTTGAAAATAATGTNGAAGAATTTNCXXXXXXXXXXATCCAAGAT
GTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCANCCAGAACCAGAATCAAATGAT
GCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCANAAAGTTGGTTTTAGACTCNCCAAAG
NATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCANTGCAAGAATCTTGCTCCAATA
GTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGA
TGCC

qdxeeikmrlqxahqqlqeqspaxyqsmksedlrkfr**sns*ys*gnanst*ksrywst
ksrr*rtinxftk*rtwfiqkstrs*treksqstrinql*ieftrrsqnxmnfy*IXSMM
NNXNNHRKVSNKQXLQEQQINSPEDIQYQLNHLNQPFQDDYHNDQTEELXDDDYNFNDQQ
INNGQFENNVEEF---

---IQDVIGGKVSPFVKSXPEPESNDAPVKVAVGTTFXKLVLDSPKXVLVEFYAPWCGXC
KNLAPIVR*ir*ilkrr*iclnrkd*c

Frame A:
qdxeeikmrlqxahqqlqeqspaxyqsmksedlrkfr**sns*ys*gnanst*ksrywst
ksrr*rtinxftk*rtwfiqkstrs*treksqstrinql*ieftrrsqnxmnfy*IXSMM
NNXNNHRKVSNKQXLQEQQINSPEDIQYQLNHLNQPFQDDYHNDQTEELXDDDYNFNDQQ
INNGQFENNVEEF---

---IQDVIGGKVSPFVKSXPEPESNDAPVKVAVGTTFXKLVLDSPKXVLVEFYAPWCGXC
KNLAPIVR*ir*ilkrr*iclnrkd*c

Frame B:
kixkklk*dyxvlinnyknnhlxsinq*nlki*gnlddevirntlkemqiqrenqdigqq
nqedkeqlxdlqnrepglyknqqdlkqekranqqelinyelnlqedhrtx*tfirsxl**
ttxtttgkfqinnxfknnklihprifnin*ii*inhskmtiimiklknxxmmiiilmink
ltmvnlkimxknx---

---skmslvvkflhllnlxqnqnqmmlqlrlplvplsxswf*txqxmfwlnstlhgavxa
rillq*ydklgeylkdvesvsivkida

Frame C:
rx*rn*neitxcsstitrtitcxlsinei*rskei*mmk*fvillrkckfnvkikilvnk
ikkiknn*xiykienlvytkinkilnkrkepinkn*simn*iykkitexyellldxlyde
qxqqpqesfk*ttxsrttn*ftrgysisiksfkstipr*ls**sn*rixr**l*f**stn
*qwsi*k*cxrix---

---prchww*sfsic*isxrtrik*css*gcrwyhfxkvgfrlxkxcfg*ilrsmvrsxq
escsnstin*vnt*ktlnlsqs*rlm

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL124 (AFL124Q) /CSM/AF/AFL1-A/AFL124Q.Seq.d/                       1507   0.0
AFE349 (AFE349Q) /CSM/AF/AFE3-C/AFE349Q.Seq.d/                       1005   0.0
AFJ569 (AFJ569Q) /CSM/AF/AFJ5-C/AFJ569Q.Seq.d/                        997   0.0
AHL872 (AHL872Q) /CSM/AH/AHL8-C/AHL872Q.Seq.d/                        993   0.0
AFC366 (AFC366Q) /CSM/AF/AFC3-C/AFC366Q.Seq.d/                        985   0.0
AFN863 (AFN863Q) /CSM/AF/AFN8-C/AFN863Q.Seq.d/                        981   0.0
CFG652 (CFG652Q) /CSM/CF/CFG6-C/CFG652Q.Seq.d/                        979   0.0
AFM384 (AFM384Q) /CSM/AF/AFM3-D/AFM384Q.Seq.d/                        973   0.0
CHC486 (CHC486Q) /CSM/CH/CHC4-D/CHC486Q.Seq.d/                        969   0.0
CFH332 (CFH332Q) /CSM/CF/CFH3-B/CFH332Q.Seq.d/                        967   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25143|U25143.1 Dictyostelium discoideum cAMP-inducible prespore protein gene, complete cds.   428   0.0    4
BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   266   1e-96  2
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    74   4e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    66   1e-06  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    66   1e-06  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    66   1e-06  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    66   1e-06  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    66   1e-06  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    62   2e-05  1
CN845821|CN845821.1 PG07008C11 Ginseng cDNA library from MeJA treated hairy root Panax ginseng cDNA clone PG07008C11 5', mRNA sequence.    58   5e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54682) RecName: Full=cAMP-inducible prespore protein D7; Flags...   149   1e-34
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...    89   2e-16
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...    88   3e-16
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...    86   1e-15
BC097044_1(BC097044|pid:none) Danio rerio protein disulfide isom...    86   1e-15
AJ440747_1(AJ440747|pid:none) Ostertagia ostertagi mRNA for prot...    85   2e-15
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...    84   4e-15
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...    84   7e-15
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...    84   7e-15
AJ271376_1(AJ271376|pid:none) Arabidopsis thaliana mRNA for ERp7...    75   9e-15

 psg: 0.76  gvh: 0.14  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for AFL124 is nuc

>AFL124F.Seq
CAAGATANTGAAGAAATTAAAATGAGATTACAANGTGCTCATCAACAATTACAAGAACAA
TCACCTGCANTCTATCAATCAATGAAATCTGAAGATCTAAGGAAATTTAGATGATGAAGT
AATTCGTAATACTCTTAAGGAAATGCAAATTCAACGTGAAAATCAAGATATTGGTCAACA
AAATCAAGAAGATAAAGAACAATTAATNGATTTACAAAATAGAGAACCTGGTTTATACAA
AAATCAACAAGATCTTAAACAAGAGAAAAGAGCCAATCAACAAGAATTAATCAATTATGA
ATTGAATTTACAAGAAGATCACAGAACANTATGAACTTTTATTAGATCNNCTCTATGATG
AACAACANCAACAACCACAGGAAAGTTTCAAATAAACAACANCTTCAAGAACAACAAATT
AATTCACCCGAGGATATTCAATATCAATTAAATCATTTAAATCAACCATTCCAAGATGAC
TATCATAATGATCAAACTGAAGAATTANNAGATGATGATTATAATTTTAATGATCAACAA
ATTAACAATGGTCAATTTGAAAATAATGTNGAAGAATTTNC----------

>AFL124Z.Seq
----------ATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCANCC
AGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCANAAAGTT
GGTTTTAGACTCNCCAAAGNATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCANTG
CAAGAATCTTGCTCCAATAGTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTG
TCTCAATCGTAAAGATTGATGCC

>AFL124P.Seq
CAAGATANTGAAGAAATTAAAATGAGATTACAANGTGCTCATCAACAATTACAAGAACAA
TCACCTGCANTCTATCAATCAATGAAATCTGAAGATCTAAGGAAATTTAGATGATGAAGT
AATTCGTAATACTCTTAAGGAAATGCAAATTCAACGTGAAAATCAAGATATTGGTCAACA
AAATCAAGAAGATAAAGAACAATTAATNGATTTACAAAATAGAGAACCTGGTTTATACAA
AAATCAACAAGATCTTAAACAAGAGAAAAGAGCCAATCAACAAGAATTAATCAATTATGA
ATTGAATTTACAAGAAGATCACAGAACANTATGAACTTTTATTAGATCNNCTCTATGATG
AACAACANCAACAACCACAGGAAAGTTTCAAATAAACAACANCTTCAAGAACAACAAATT
AATTCACCCGAGGATATTCAATATCAATTAAATCATTTAAATCAACCATTCCAAGATGAC
TATCATAATGATCAAACTGAAGAATTANNAGATGATGATTATAATTTTAATGATCAACAA
ATTAACAATGGTCAATTTGAAAATAATGTNGAAGAATTTNC----------ATCCAAGAT
GTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCANCCAGAACCAGAATCAAATGAT
GCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCANAAAGTTGGTTTTAGACTCNCCAAAG
NATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCANTGCAAGAATCTTGCTCCAATA
GTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGA
TGCC