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Dicty_cDB
Contig List | Clone List | cDNA Library List
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Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL122
Library AF
(Link to library)
Clone ID AFL122
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11144-1
Original site URL
Representative seq. ID AFL122P
(Link to Original site)
Representative DNA sequence 
>AFL122 (AFL122Q) /CSM/AF/AFL1-A/AFL122Q.Seq.d/
TTTATTGTTTTTTTATTTTATTTTTATTATTATTATTTCTATTATTATTATTATTTCTAT
TATTATTATTATTTTAATTTTTAATTTCTATTATTCACAATTATTAATTTTTTATTTATT
TTTATTTTTATTTTTTTAAATTCTTCTTTTTAAAGTTACTATTAAATGGATAGTAATAAT
AATAATAACAATGAAAATGAAGCTTTTAGTGGAGCCAGTGAAAGTTCAGAATTTCGTAAA
ATTGTAGAAGAAAATGAAAATGAAAGAGAATTTGAACAATCAAATCCTTCTCCACCAGAA
TATTCAAACTATGAAAATAAAGATGATGGAATTAATTTAGAAACAATTAATCCAAATATT
TCATTAGATAATAATAATAATAATAATCAAAATAATCAAAATAATCAAAATAATAATAAT
AATAATAATAATCAAAATAATAATATTATTAATAATTTAAATAAAAAGAATAAAAAAAGA
TCAACATTTAAAAATAGAATTGATTTTTCATTTAAAGATATAAATCATTATGTTCAAATT
ACCGAAAAAGGTAAAAAGAAAAAAATATCAAAACAAATTTTAACAAATATTAATGGTCAT
ATTGAGAGTGGAACAATTTTTGCAATTATGGGACCATCGGGTGCGGGTAAAACCACATTG
TTGGATATTCTAGCACATAGATTAAATATTAATGGTAGTGGAACAATGTATTTGAATGGT
AATAAATCTGATTTCAATATTTTTAAAAAACTTTGTGGTTATGTAACACAAAGTGATTCA
TTGATGCCATCATTGACCGTAAGAGAGACATTGAATTTTTATGCTCAXXXXXXXXXXGAG
ACATTGAATTTTTATGCTCAACTAAAGATGCCAAGAGATGTTCCATTAAAAGAAAAACTT
CAAAGAGTTCAAGATATTATCGATGAAATGGGCTTAAATCGTTGTGCTGATACATTGGTT
GGTACTGCTGATAATAAAATTAGAGGTATCTCTGGTGGTGAAAGAAGAAGAGTGACAATC
TCCATAGAGTTGTTAACTGGTCCATCCGTGATTCTATTGGATGAACCAACTTCAGGTTTA
GATGCTTCAACAAGTTTCTATGTAATGTCTGCATTGAAAAAGTTGGCTAAATCTGGTAGA
ACTATCATTTGCACAATTCATCAACCACGTTCAAATATTTATGATATGTTTGATAACCTT
TTATTATTAGGTGATGGTAATACCATCTATTATGGTAAAGCAAATAAAGCATTGGAATAT
TTCAATGCAAATGGTTATCATTGTAGTGAAAAAACAAATCCAGCTGATTTCTTTTTAGAT
TTAATTAATACTCAAGTTGAAGATCAAGCTGATAGTGATGATGATGACTACAATGATGAG
GAGGAAGAAATTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGCTGGTGGCATTGAAGAT
ATTGGTATCTCAATTTCACCAACTATGAATGGTTCTGCTGTTATAATATTAAAAATAANA
ACTAAAACAACAACAACAACAACAACAACAACAACAACAATCTACAGA
sequence update 2003. 5. 2
Translated Amino Acid sequence 
fivflfyfyyyyfyyyyyfyyyyyfnf*fllftiinflfifififlnssf*syy*MDSNN
NNNNENEAFSGASESSEFRKIVEENENEREFEQSNPSPPEYSNYENKDDGINLETINPNI
SLDNNNNNNQNNQNNQNNNNNNNNQNNNIINNLNKKNKKRSTFKNRIDFSFKDINHYVQI
TEKGKKKKISKQILTNINGHIESGTIFAIMGPSGAGKTTLLDILAHRLNINGSGTMYLNG
NKSDFNIFKKLCGYVTQSDSLMPSLTVRETLNFYA---

---ETLNFYAQLKMPRDVPLKEKLQRVQDIIDEMGLNRCADTLVGTADNKIRGISGGERR
RVTISIELLTGPSVILLDEPTSGLDASTSFYVMSALKKLAKSGRTIICTIHQPRSNIYDM
FDNLLLLGDGNTIYYGKANKALEYFNANGYHCSEKTNPADFFLDLINTQVEDQADSDDDD
YNDEEEEIGGGGGGSGGGAGGIEDIGISISPTMNGSAVIILKIXTKTTTTTTTTTTTIYR
Translated Amino Acid sequence (All Frames) 
Frame A:
fivflfyfyyyyfyyyyyfyyyyyfnf*fllftiinflfifififlnssf*syy*MDSNN
NNNNENEAFSGASESSEFRKIVEENENEREFEQSNPSPPEYSNYENKDDGINLETINPNI
SLDNNNNNNQNNQNNQNNNNNNNNQNNNIINNLNKKNKKRSTFKNRIDFSFKDINHYVQI
TEKGKKKKISKQILTNINGHIESGTIFAIMGPSGAGKTTLLDILAHRLNINGSGTMYLNG
NKSDFNIFKKLCGYVTQSDSLMPSLTVRETLNFYA---

---ETLNFYAQLKMPRDVPLKEKLQRVQDIIDEMGLNRCADTLVGTADNKIRGISGGERR
RVTISIELLTGPSVILLDEPTSGLDASTSFYVMSALKKLAKSGRTIICTIHQPRSNIYDM
FDNLLLLGDGNTIYYGKANKALEYFNANGYHCSEKTNPADFFLDLINTQVEDQADSDDDD
YNDEEEEIGGGGGGSGGGAGGIEDIGISISPTMNGSAVIILKIXTKTTTTTTTTTTTIYR

Frame B:
llffyfifiiiisiiiiisiiiiilifnfyysqllifylflflff*illfkvtikwivii
iitmkmkllvepvkvqnfvkl*kkmkmkenlnnqillhqniqtmkikmmeli*kqliqif
h*iiiiiiikiikiikiiiiiiiikiiillii*ikrikkdqhlkielifhlki*iimfkl
pkkvkrkkyqnkf*qilmvilrveqflqlwdhrvrvkphcwif*hid*ilmvveqci*mv
inlisiflknfvvm*hkvih*chh*p*erh*ifml---

---rh*ifmln*rcqemfh*kknfkefkilsmkwa*ivvlihwlvlliiklevslvvkee
e*qsp*sc*lvhp*fywmnqlqv*mlqqvsm*clh*kswlnlvelsfaqfinhvqifmic
litfyy*vmvipsimvkqikhwnismqmviivvkkqiqlisf*i*lilklkiklivmmmt
tmmrrkklvvvvvvvvvvlvalkilvsqfhql*mvlll*y*k*xlkqqqqqqqqqqqst

Frame C:
ycffilfllllflllllflllllf*flisiihny*ffiyfyfyffkffflklllng****
**q*k*sf*wsq*kfris*ncrrk*k*kri*tiksfstrifkl*k*r*wn*frnn*skyf
ir******sk*sk*sk*******sk**yy**fk*ke*kkini*k*n*ffi*rykslcsny
rkr*kekniktnfnky*wsy*ewnnfcnygtigcg*nhivgysst*iky*w*wnnvfew*
*i*fqyf*ktlwlcntk*fidaiidrkrdieflcs---

---dieflcstkdakrcsikrktskssryyr*nglkslc*yigwyc***n*rylww*kkk
sdnlhrvvnwsirdsig*tnfrfrcfnkflcnvciekvg*iw*nyhlhnssttfkyl*yv
**pfiir*w*yhllw*sk*sigifqckwlsl**knkss*flfrfn*yss*rss******l
q**ggrnwwwwww*wwwcwwh*rywylnftnyewfccyniknxn*nnnnnnnnnnnnlq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL122 (AFL122Q) /CSM/AF/AFL1-A/AFL122Q.Seq.d/                       1578   0.0
AFM677 (AFM677Q) /CSM/AF/AFM6-D/AFM677Q.Seq.d/                        946   0.0
VHG666 (VHG666Q) /CSM/VH/VHG6-C/VHG666Q.Seq.d/                        519   e-146
CHJ754 (CHJ754Q) /CSM/CH/CHJ7-C/CHJ754Q.Seq.d/                        519   e-146
SHJ559 (SHJ559Q) /CSM/SH/SHJ5-C/SHJ559Q.Seq.d/                        456   e-127
CHN329 (CHN329Q) /CSM/CH/CHN3-B/CHN329Q.Seq.d/                        456   e-127
CHK501 (CHK501Q) /CSM/CH/CHK5-A/CHK501Q.Seq.d/                        456   e-127
CHE614 (CHE614Q) /CSM/CH/CHE6-A/CHE614Q.Seq.d/                        456   e-127
AHQ796 (AHQ796Q) /CSM/AH/AHQ7-D/AHQ796Q.Seq.d/                        456   e-127
AHQ174 (AHQ174Q) /CSM/AH/AHQ1-D/AHQ174Q.Seq.d/                        456   e-127
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF482380|AF482380.1 Dictyostelium discoideum ABC transporter AbcG1 (abcG1) gene, complete cds.  1098   0.0    5
AF482384|AF482384.1 Dictyostelium discoideum ABC transporter AbcG5 (abcG5) gene, complete cds.    66   6e-14  7
AF482385|AF482385.1 Dictyostelium discoideum ABC transporter AbcG6 (abcG6) gene, partial cds.    66   8e-12  6
AF482391|AF482391.1 Dictyostelium discoideum ABC transporter AbcG13 (abcG13) gene, complete cds.    46   9e-10  7
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 5 unordered pieces.    42   5e-09  14
AL049183|AL049183.5 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P6.    38   9e-09  16
AF362369|AF362369.2 Dictyostelium discoideum histidine kinase DhkG (dhkG) gene, complete cds.    44   9e-09  8
AC116921|AC116921.2 Dictyostelium discoideum chromosome 2 map 4624505-4657775 strain AX4, complete sequence.    40   1e-08  10
AC004157|AC004157.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    38   2e-08  16
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36   3e-08  23
dna update 2004. 5.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF677363_1(EF677363|pid:none) Picea sitchensis clone WS02766_G11...   227   1e-57
AC068950_12(AC068950|pid:none) Oryza sativa chromosome 10 BAC OS...   225   3e-57
(Q80W57) RecName: Full=ATP-binding cassette sub-family G member ...   224   5e-57
(Q93YS4) RecName: Full=White-brown complex homolog protein 23;  ...   224   5e-57
AY089996_1(AY089996|pid:none) Rattus norvegicus ATP-binding cass...   224   5e-57
(Q7TMS5) RecName: Full=ATP-binding cassette sub-family G member ...   224   8e-57
BT030709_1(BT030709|pid:none) Bos taurus ATP-binding cassette, s...   223   2e-56
CR942670_1(CR942670|pid:none) Xenopus tropicalis finished cDNA, ...   223   2e-56
AY093054_1(AY093054|pid:none) Arabidopsis thaliana unknown prote...   221   7e-56
AY255521_1(AY255521|pid:none) Gossypium hirsutum ABC transporter...   221   7e-56
protein update 2009. 3.28
PSORT 

 psg: 0.75  gvh: 0.20  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for AFL122 is nuc
5' end seq. ID AFL122F
5' end seq. 
>AFL122F.Seq
TTTATTGTTTTTTTATTTTATTTTTATTATTATTATTTCTATTATTATTATTATTTCTAT
TATTATTATTATTTTAATTTTTAATTTCTATTATTCACAATTATTAATTTTTTATTTATT
TTTATTTTTATTTTTTTAAATTCTTCTTTTTAAAGTTACTATTAAATGGATAGTAATAAT
AATAATAACAATGAAAATGAAGCTTTTAGTGGAGCCAGTGAAAGTTCAGAATTTCGTAAA
ATTGTAGAAGAAAATGAAAATGAAAGAGAATTTGAACAATCAAATCCTTCTCCACCAGAA
TATTCAAACTATGAAAATAAAGATGATGGAATTAATTTAGAAACAATTAATCCAAATATT
TCATTAGATAATAATAATAATAATAATCAAAATAATCAAAATAATCAAAATAATAATAAT
AATAATAATAATCAAAATAATAATATTATTAATAATTTAAATAAAAAGAATAAAAAAAGA
TCAACATTTAAAAATAGAATTGATTTTTCATTTAAAGATATAAATCATTATGTTCAAATT
ACCGAAAAAGGTAAAAAGAAAAAAATATCAAAACAAATTTTAACAAATATTAATGGTCAT
ATTGAGAGTGGAACAATTTTTGCAATTATGGGACCATCGGGTGCGGGTAAAACCACATTG
TTGGATATTCTAGCACATAGATTAAATATTAATGGTAGTGGAACAATGTATTTGAATGGT
AATAAATCTGATTTCAATATTTTTAAAAAACTTTGTGGTTATGTAACACAAAGTGATTCA
TTGATGCCATCATTGACCGTAAGAGAGACATTGAATTTTTATGCTCANNNNNNNNNN
Length of 5' end seq. 837
3' end seq. ID AFL122Z
3' end seq. 
>AFL122Z.Seq
----------GAGACATTGAATTTTTATGCTCAACTAAAGATGCCAAGAGATGTTCCATT
AAAAGAAAAACTTCAAAGAGTTCAAGATATTATCGATGAAATGGGCTTAAATCGTTGTGC
TGATACATTGGTTGGTACTGCTGATAATAAAATTAGAGGTATCTCTGGTGGTGAAAGAAG
AAGAGTGACAATCTCCATAGAGTTGTTAACTGGTCCATCCGTGATTCTATTGGATGAACC
AACTTCAGGTTTAGATGCTTCAACAAGTTTCTATGTAATGTCTGCATTGAAAAAGTTGGC
TAAATCTGGTAGAACTATCATTTGCACAATTCATCAACCACGTTCAAATATTTATGATAT
GTTTGATAACCTTTTATTATTAGGTGATGGTAATACCATCTATTATGGTAAAGCAAATAA
AGCATTGGAATATTTCAATGCAAATGGTTATCATTGTAGTGAAAAAACAAATCCAGCTGA
TTTCTTTTTAGATTTAATTAATACTCAAGTTGAAGATCAAGCTGATAGTGATGATGATGA
CTACAATGATGAGGAGGAAGAAATTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGCTGG
TGGCATTGAAGATATTGGTATCTCAATTTCACCAACTATGAATGGTTCTGCTGTTATAAT
ATTAAAAATAANAACTAAAACAACAACAACAACAACAACAACAACAACAACAATCTACAG
A
Length of 3' end seq. 711
Connected seq. ID AFL122P
Connected seq. 
>AFL122P.Seq
TTTATTGTTTTTTTATTTTATTTTTATTATTATTATTTCTATTATTATTATTATTTCTAT
TATTATTATTATTTTAATTTTTAATTTCTATTATTCACAATTATTAATTTTTTATTTATT
TTTATTTTTATTTTTTTAAATTCTTCTTTTTAAAGTTACTATTAAATGGATAGTAATAAT
AATAATAACAATGAAAATGAAGCTTTTAGTGGAGCCAGTGAAAGTTCAGAATTTCGTAAA
ATTGTAGAAGAAAATGAAAATGAAAGAGAATTTGAACAATCAAATCCTTCTCCACCAGAA
TATTCAAACTATGAAAATAAAGATGATGGAATTAATTTAGAAACAATTAATCCAAATATT
TCATTAGATAATAATAATAATAATAATCAAAATAATCAAAATAATCAAAATAATAATAAT
AATAATAATAATCAAAATAATAATATTATTAATAATTTAAATAAAAAGAATAAAAAAAGA
TCAACATTTAAAAATAGAATTGATTTTTCATTTAAAGATATAAATCATTATGTTCAAATT
ACCGAAAAAGGTAAAAAGAAAAAAATATCAAAACAAATTTTAACAAATATTAATGGTCAT
ATTGAGAGTGGAACAATTTTTGCAATTATGGGACCATCGGGTGCGGGTAAAACCACATTG
TTGGATATTCTAGCACATAGATTAAATATTAATGGTAGTGGAACAATGTATTTGAATGGT
AATAAATCTGATTTCAATATTTTTAAAAAACTTTGTGGTTATGTAACACAAAGTGATTCA
TTGATGCCATCATTGACCGTAAGAGAGACATTGAATTTTTATGCTCA----------GAG
ACATTGAATTTTTATGCTCAACTAAAGATGCCAAGAGATGTTCCATTAAAAGAAAAACTT
CAAAGAGTTCAAGATATTATCGATGAAATGGGCTTAAATCGTTGTGCTGATACATTGGTT
GGTACTGCTGATAATAAAATTAGAGGTATCTCTGGTGGTGAAAGAAGAAGAGTGACAATC
TCCATAGAGTTGTTAACTGGTCCATCCGTGATTCTATTGGATGAACCAACTTCAGGTTTA
GATGCTTCAACAAGTTTCTATGTAATGTCTGCATTGAAAAAGTTGGCTAAATCTGGTAGA
ACTATCATTTGCACAATTCATCAACCACGTTCAAATATTTATGATATGTTTGATAACCTT
TTATTATTAGGTGATGGTAATACCATCTATTATGGTAAAGCAAATAAAGCATTGGAATAT
TTCAATGCAAATGGTTATCATTGTAGTGAAAAAACAAATCCAGCTGATTTCTTTTTAGAT
TTAATTAATACTCAAGTTGAAGATCAAGCTGATAGTGATGATGATGACTACAATGATGAG
GAGGAAGAAATTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGCTGGTGGCATTGAAGAT
ATTGGTATCTCAATTTCACCAACTATGAATGGTTCTGCTGTTATAATATTAAAAATAANA
ACTAAAACAACAACAACAACAACAACAACAACAACAACAATCTACAGA
Length of connected seq. 1538
Full length Seq ID -
Full length Seq. -
Length of full length seq. -