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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK889
Library AF
(Link to library)
Clone ID AFK889
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFK889Z
(Link to Original site)
Representative DNA sequence 
>AFK889 (AFK889Q) /CSM/AF/AFK8-D/AFK889Q.Seq.d/
XXXXXXXXXXTATGAATCAATCGCCGTTCCATTAGCTTATTTATTCATTGACTCCACTCA
AGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGC
CGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTC
TGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTT
CGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGG
TGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGT
TAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTT
GGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAA
ATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTC
AAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGC
TGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGT
TGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCATCATCTCAAACNANATAA
TGTNAATCTAAAAAAGATTCTTCTGCTAAACANANAATTATAAATAATTAATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---ESIAVPLAYLFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSG
KVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVK
VAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSN
DVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKXXNV
NLKKILLLNXXL*iink
Translated Amino Acid sequence (All Frames) 
Frame A:
---mnqspfh*liysltplkitlkfskm*rkslphkkvmpfsvgsi*rnshnkqliwvsl
vklfqpslliqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlql
rlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptq
mmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlp*iqitiisnxim
xi*krffc*txnyk*li


Frame B:
---*inrrsislfih*lhsr*hsssrrckenryltkr*crfllgryeeipttsnsygslw
*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*
gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk
*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rchefklpssqtx*c
xskkdssakxxiinn**


Frame C:
---ESIAVPLAYLFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSG
KVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVK
VAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSN
DVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKXXNV
NLKKILLLNXXL*iink
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK889 (AFK889Q) /CSM/AF/AFK8-D/AFK889Q.Seq.d/                       1487   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1421   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1417   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1415   0.0
AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/                       1407   0.0
AFK319 (AFK319Q) /CSM/AF/AFK3-A/AFK319Q.Seq.d/                       1403   0.0
AFK148 (AFK148Q) /CSM/AF/AFK1-B/AFK148Q.Seq.d/                       1392   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1380   0.0
AFJ720 (AFJ720Q) /CSM/AF/AFJ7-A/AFJ720Q.Seq.d/                       1376   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1374   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   6e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   6e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   6e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   6e-08  1
dna update 2004. 8. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   189   1e-46
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   186   9e-46
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   180   4e-44
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   171   2e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   1e-39
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   162   1e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   162   1e-38
(Q10057) RecName: Full=Putative protein disulfide-isomerase C1F5...   158   2e-37
U82634_1(U82634|pid:none) Alternaria alternata putative protein ...   156   8e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
protein update 2009. 4.25
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK889 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFK889Z
3' end seq. 
>AFK889Z.Seq
----------TATGAATCAATCGCCGTTCCATTAGCTTATTTATTCATTGACTCCACTCA
AGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGC
CGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTC
TGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTT
CGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGG
TGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGT
TAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTT
GGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAA
ATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTC
AAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGC
TGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGT
TGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCATCATCTCAAACNANATAA
TGTNAATCTAAAAAAGATTCTTCTGCTAAACANANAATTATAAATAATTAATAAA
Length of 3' end seq. 765
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -