>AFK884 (AFK884Q) /CSM/AF/AFK8-D/AFK884Q.Seq.d/
TTTTTCCAAACAAATAAAAAAATAATATAATATAATATAATTAAATATATAAAATGAGCA
ATGAAGTTATTAATGTATTAATTACTGGTGCAGCAGGTCAAATTGCATATTCATTAATTT
TCAATGTATGTAAAGGTGATATGTTTGGTTTAGATCAACGTATTAAATTACATCTTTTAG
ATATTCTACAAATGGTAGACTCATTGAAAGGTATTGTAATGGAGATTCAAGATGGTGCAT
ATCCATTGATCGCTGATACAGTTATAACTGCCGATGTTAAAGAAGCATTCACTGGTGTAC
ATTATGCAATTTTAGTTGGTGCTATGCCACGTAGAGAAGGTATGGAAAGAGCTGATCTTT
TGAAAGCAAACGCAGCAATCTTCAAAGTTCAAGGTAAAGCACTCGCCGAACATGCAAATA
AGAATGTTAAAGTATTGGTAGTTGGTAATCCAGCCAATACCAATGCACTCATTGCACAAG
TATCAGCCAATGGCATTCCAAAGGAGAACTTTACATGTCTCACTCGTCTCGATCAAAATC
GTGCCAAATCTCAAATAGCCTTGAAAGCAGGTGTAAATGTCAAGGATGTCCACAATGTAA
TTATCTGGGGCAACCATTCATCAACTCAATXXXXXXXXXX

fpnk*knnii*yn*IYKMSNEVINVLITGAAGQIAYSLIFNVCKGDMFGLDQRIKLHLLD
ILQMVDSLKGIVMEIQDGAYPLIADTVITADVKEAFTGVHYAILVGAMPRREGMERADLL
KANAAIFKVQGKALAEHANKNVKVLVVGNPANTNALIAQVSANGIPKENFTCLTRLDQNR
AKSQIALKAGVNVKDVHNVIIWGNHSSTQ---

Frame A:
ffqtnkkii*yniikyik*amkllmy*llvqqvklhih*fsmyvkviclv*invlnyif*
ifykw*th*kvl*wrfkmvhih*sliql*lpmlkkhslvyimqf*lvlchvekvwkelif
*kqtqqsskfkvkhspnmqirmlkyw*lviqpipmhslhkyqpmafqrrtlhvslvsiki
vpnlk*p*kqv*msrmstm*lsgatihql---


Frame B:
fskqikk*ynii*lni*neq*sy*cinywcsrsncifinfqcm*r*yvwfrsty*itsfr
ystngrlierycngdsrwcisidr*ysyncrc*rsihwctlcnfswcyat*rrygks*sf
eskrsnlqssr*strrtck*ec*sigsw*ssqyqcthctsisqwhskgelymshssrsks
cqisnslesrckcqgcpqcnylgqpfins---


Frame C:
fpnk*knnii*yn*IYKMSNEVINVLITGAAGQIAYSLIFNVCKGDMFGLDQRIKLHLLD
ILQMVDSLKGIVMEIQDGAYPLIADTVITADVKEAFTGVHYAILVGAMPRREGMERADLL
KANAAIFKVQGKALAEHANKNVKVLVVGNPANTNALIAQVSANGIPKENFTCLTRLDQNR
AKSQIALKAGVNVKDVHNVIIWGNHSSTQ---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK884 (AFK884Q) /CSM/AF/AFK8-D/AFK884Q.Seq.d/                       1140   0.0
VFL218 (VFL218Q) /CSM/VF/VFL2-A/VFL218Q.Seq.d/                       1132   0.0
VFD713 (VFD713Q) /CSM/VF/VFD7-A/VFD713Q.Seq.d/                       1132   0.0
SFK157 (SFK157Q) /CSM/SF/SFK1-C/SFK157Q.Seq.d/                       1132   0.0
VFK307 (VFK307Q) /CSM/VF/VFK3-A/VFK307Q.Seq.d/                       1124   0.0
VFE489 (VFE489Q) /CSM/VF/VFE4-D/VFE489Q.Seq.d/                       1124   0.0
AHD828 (AHD828Q) /CSM/AH/AHD8-B/AHD828Q.Seq.d/                       1124   0.0
SFJ524 (SFJ524Q) /CSM/SF/SFJ5-A/SFJ524Q.Seq.d/                       1118   0.0
AFN235 (AFN235Q) /CSM/AF/AFN2-B/AFN235Q.Seq.d/                       1108   0.0
AFN387 (AFN387Q) /CSM/AF/AFN3-D/AFN387Q.Seq.d/                       1102   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CN753239|CN753239.1 ApHL3LD-XVII-A4 ApHL3LD Acyrthosiphon pisum cDNA cloneApHL3LDXVIIA4 5', mRNA sequence.    44   1e-13  4
BG612351|BG612351.1 602613689F1 NIH_MGC_60 Homo sapiens cDNA clone IMAGE:4738871 5', mRNA sequence.    48   4e-13  3
CN584208|CN584208.1 USDA-FP_127276 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP056_C03, mRNA sequence.    42   1e-11  4
CD469580|CD469580.1 LeukoS2_4_G09.g1_A024 Stimulated peripheral blood leukocytes S2 Equus caballus cDNA clone LeukoS2_4_G09_A024 5', mRNA sequence.    46   2e-11  4
CN756155|CN756155.1 ID0AAA17DF02RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA17DF02 5', mRNA sequence.    42   2e-11  4
CN754652|CN754652.1 ID0AAA13CE10RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA13CE10 5', mRNA sequence.    42   3e-11  4
CN764322|CN764322.1 ID0AAA9CD06RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA9CD06 5', mRNA sequence.    42   3e-11  4
BG428038|BG428038.1 602501446F1 NIH_MGC_75 Homo sapiens cDNA clone IMAGE:4615067 5', mRNA sequence.    48   3e-11  3
CN585017|CN585017.1 USDA-FP_128086 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP067_B09, mRNA sequence.    42   4e-11  4
CN763349|CN763349.1 ID0AAA6DC04RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA6DC04 5', mRNA sequence.    42   4e-11  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT043497_1(BT043497|pid:none) Salmo salar clone HM4_0203 malate ...   240   3e-62
EU569765_1(EU569765|pid:none) Ctenopharyngodon idella cytosolic ...   240   3e-62
DQ215422_1(DQ215422|pid:none) Taeniopygia guttata clone 0058P000...   239   4e-62
DQ215421_1(DQ215421|pid:none) Taeniopygia guttata clone 0061P002...   239   4e-62
BT045320_1(BT045320|pid:none) Salmo salar clone ssal-rgf-518-137...   238   1e-61
BC059124_1(BC059124|pid:none) Rattus norvegicus malate dehydroge...   234   1e-60
(O88989) RecName: Full=Malate dehydrogenase, cytoplasmic;       ...   234   1e-60
DEMSMC(S02654;S00195) malate dehydrogenase (EC 1.1.1.37), cytoso...   234   1e-60
AF390559_1(AF390559|pid:none) Sphyraena idiastes cytosolic malat...   234   1e-60
(P14152) RecName: Full=Malate dehydrogenase, cytoplasmic;       ...   234   1e-60

 psg: 0.91  gvh: 0.53  alm: 0.38  top: 0.40  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system

>> prediction for AFK884 is cyt

>AFK884F.Seq
TTTTTCCAAACAAATAAAAAAATAATATAATATAATATAATTAAATATATAAAATGAGCA
ATGAAGTTATTAATGTATTAATTACTGGTGCAGCAGGTCAAATTGCATATTCATTAATTT
TCAATGTATGTAAAGGTGATATGTTTGGTTTAGATCAACGTATTAAATTACATCTTTTAG
ATATTCTACAAATGGTAGACTCATTGAAAGGTATTGTAATGGAGATTCAAGATGGTGCAT
ATCCATTGATCGCTGATACAGTTATAACTGCCGATGTTAAAGAAGCATTCACTGGTGTAC
ATTATGCAATTTTAGTTGGTGCTATGCCACGTAGAGAAGGTATGGAAAGAGCTGATCTTT
TGAAAGCAAACGCAGCAATCTTCAAAGTTCAAGGTAAAGCACTCGCCGAACATGCAAATA
AGAATGTTAAAGTATTGGTAGTTGGTAATCCAGCCAATACCAATGCACTCATTGCACAAG
TATCAGCCAATGGCATTCCAAAGGAGAACTTTACATGTCTCACTCGTCTCGATCAAAATC
GTGCCAAATCTCAAATAGCCTTGAAAGCAGGTGTAAATGTCAAGGATGTCCACAATGTAA
TTATCTGGGGCAACCATTCATCAACTCAAT----------