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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK881
Library AF
(Link to library)
Clone ID AFK881
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFK881P
(Link to Original site)
Representative DNA sequence 
>AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAXXXXXXXXXXGAATGTCATC
AGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCA
ATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTAC
ACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGT
TACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAA
TTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCA
TTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATT
GTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAA
GCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGT
GCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAA
ACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACT
GGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAA
GAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCA
TAATATGNAAACAAAAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHA
GNKLAMQEFMILP---

---NVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASE
FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTA
SVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMV
SHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFR
KF*fys*yxnk
Translated Amino Acid sequence (All Frames) 
Frame A:
thlllyfflslsiyisffkmsviksikareildsrgnptvevdlytekdgvvssfraavp
sgastgiyeavelrdgdksrylgkgvlkaiknileviqpavigksvsdqaaidklmidld
gtpnkgklganailavslavcragaadrnlplykyiseiagtkmrlpvpafnvingwftc
r**isyariydft---

---echqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*
iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*ins
is*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg
*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcwfnfp
*ililliixkqn

Frame B:
ptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHA
GNKLAMQEFMILP---

---NVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASE
FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTA
SVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMV
SHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFR
KF*fys*yxnk

Frame C:
plitlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssi
rcinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrw
ypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwvvhmq
vin*lcknl*fy---

---mssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlpln
sklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*qh
qlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswl
aivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmlv*lsv
nfnfthnmxtk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       2533   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       2512   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1475   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1473   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1473   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1473   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1467   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1461   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1461   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1459   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   3e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           70   6e-68  11
CN587505|CN587505.1 USDA-FP_130580 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP098_G04, mRNA sequence.   101   6e-59  6
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   2e-57  9
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   4e-57  10
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   4e-57  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   4e-57  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   4e-55  9
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   3e-48  5
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   4e-46  5
dna update 2004. 8. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   755   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   533   e-150
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   524   e-147
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   514   e-144
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   513   e-144
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   513   e-144
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   512   e-143
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   512   e-143
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   510   e-143
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   509   e-143
protein update 2009. 4.25
PSORT 

 psg: 0.75  gvh: 0.51  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFK881 is cyt
5' end seq. ID AFK881F
5' end seq. 
>AFK881F.Seq
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCA----------
Length of 5' end seq. 580
3' end seq. ID AFK881Z
3' end seq. 
>AFK881Z.Seq
----------GAATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATG
AAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTG
CCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTG
AATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAG
CTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAA
TCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAG
CATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTA
AGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTT
CAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGG
TTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAG
GTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATC
AACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCC
GTAAATTTTAATTTTACTCATAATATGNAAACAAAAC
Length of 3' end seq. 747
Connected seq. ID AFK881P
Connected seq. 
>AFK881P.Seq
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCA----------GAATGTCATC
AGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCA
ATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTAC
ACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGT
TACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAA
TTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCA
TTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATT
GTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAA
GCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGT
GCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAA
ACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACT
GGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAA
GAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCA
TAATATGNAAACAAAAC
Length of connected seq. 1327
Full length Seq ID -
Full length Seq. -
Length of full length seq. -