>SSC516 (SSC516Q) /CSM/SS/SSC5-A/SSC516Q.Seq.d/
AACTGAATCCTTCAAATTCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGGTAC
ATACATTGTCATTCCATCAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAACACT
TTTCACCGATGATAAGAATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGTTGA
ACAAGTTAAAGGTACTTGGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAACTTT
CTTCAATAATCCACAATTCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCATCGC
CGTTCAATTGATTCAAGATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTTATC
TCGTGATTCTCATTCTGAACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTTTTCAC
TAAAACTTCAAATTGGGAAAAGAGAAATGATATCGTTTGTCGTCTTCATGTTAAACCAGA
ATCACCAAGAGAATTCACTATCATTCCTTCAACTTTTGATCCAAGTGTTAATCGTTCATT
CAAACTTCAAGTTATTCTGATGTTTCAATTTCATTAGATGAAATTGAACAAAAAGATGAA
TCCTCTGATTCTGAACAAAATGATGATTAXXXXXXXXXX

TESFKFSCSTGCTLTLDEGTYIVIPSTYDHGIEGAFHLTLFTDDKNATFQPLTNAFQEVE
QVKGTWVGKSAGGSPNQPTFFNNPQFHLKVPASDKDEVIAVQLIQDSTIADEGIGFIVLS
RDSHSEPLTAQDFQNEMVFTKTSNWEKRNDIVCRLHVKPESPREFTIIPSTFDPSVNRSF
KLQVILMFQFH*mklnkkmnplilnkmmi---

Frame A:
n*ilqillfhwlhfnprrryihchsinl*swyrrcfpfntfhr**echlptinkrfprg*
ts*rylgw*icwwfskstnflq*stipfestcl**r*shrrsidsrfnhcr*rywfhcfi
s*fsf*tidcsrfpk*ngfh*nfklgkek*yrlsssc*tritkrihyhsfnf*skc*sfi
qtssysdvsisldeieqkdessdseqndd---


Frame B:
TESFKFSCSTGCTLTLDEGTYIVIPSTYDHGIEGAFHLTLFTDDKNATFQPLTNAFQEVE
QVKGTWVGKSAGGSPNQPTFFNNPQFHLKVPASDKDEVIAVQLIQDSTIADEGIGFIVLS
RDSHSEPLTAQDFQNEMVFTKTSNWEKRNDIVCRLHVKPESPREFTIIPSTFDPSVNRSF
KLQVILMFQFH*mklnkkmnplilnkmmi---


Frame C:
lnpsnslvplval*pstkvhtlsfhqlmimvskvlsi*hfspmirmppsnh*qtlskrln
klkvlglvnllvvlqinqlssiihnsi*kylplikmksspfn*fkiqplpmkvlvslfyl
vililnh*llkiskmkwfslklqigkremisfvvfmlnqnhqenslsflqlliqvlivhs
nfklf*cfnfir*n*tkr*il*f*tk**---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSC516 (SSC516Q) /CSM/SS/SSC5-A/SSC516Q.Seq.d/                       1247   0.0
SLC787 (SLC787Q) /CSM/SL/SLC7-D/SLC787Q.Seq.d/                       1233   0.0
VFL249 (VFL249Q) /CSM/VF/VFL2-C/VFL249Q.Seq.d/                       1132   0.0
SLF205 (SLF205Q) /CSM/SL/SLF2-A/SLF205Q.Seq.d/                       1130   0.0
SFH493 (SFH493Q) /CSM/SF/SFH4-D/SFH493Q.Seq.d/                       1110   0.0
VHL339 (VHL339Q) /CSM/VH/VHL3-B/VHL339Q.Seq.d/                       1074   0.0
SLC473 (SLC473Q) /CSM/SL/SLC4-D/SLC473Q.Seq.d/                        823   0.0
SFD653 (SFD653Q) /CSM/SF/SFD6-C/SFD653Q.Seq.d/                         90   5e-17
VFO135 (VFO135Q) /CSM/VF/VFO1-B/VFO135Q.Seq.d/                         40   0.040
VFM323 (VFM323Q) /CSM/VF/VFM3-A/VFM323Q.Seq.d/                         40   0.040

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF525418|AF525418.1 Dictyostelium discoideum calpain-like cysteine protease (cplA) mRNA, complete cds.  1096  0.0  2
BQ667603|BQ667603.1 pb55h10.y1 Anc caninum L3 serum stim pAMP1 v1 Chiapelli McCarter Ancylostoma caninum cDNA 5' similar to TR:O18000 O18000 R11H6.1 PROTEIN. [1] ;contains Alu repetitive element;, mRNA sequence.    44  0.029  2
AC079034|AC079034.34 Homo sapiens 12 BAC RP11-652N17 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38  1.4  3
BG425862|BG425862.1 602492222F1 NIH_MGC_75 Homo sapiens cDNA clone IMAGE:4606229 5', mRNA sequence.    44  2.1  1
BP025179|BP025179.1 Ciona intestinalis cDNA, clone:cits36j01, 5' end, single read.    34  3.0  2
AJ494199|AJ494199.1 Ciona intestinalis EST, clone CION00699.      34  3.1  2
AF202987|AF202987.1 Petroselinum crispum acyl-CoA oxidase (ACO) gene, complete cds.    42  3.4  2
AC118435|AC118435.4 Rattus norvegicus clone CH230-400J1, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    38  3.5  5
AC109215|AC109215.4 Mus musculus clone RP23-354C17, WORKING DRAFT SEQUENCE, 8 ordered pieces.    38  4.5  3
AV971191|AV971191.1 Ciona intestinalis cDNA, clone:cicl21d17, 5' end, single read.    34  4.6  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC091999_1(BC091999|pid:none) Danio rerio CAPN1 protein, mRNA (c...    53   6e-06
AL954831_9(AL954831|pid:none) Zebrafish DNA sequence from clone ...    51   2e-05
BC077421_1(BC077421|pid:none) Xenopus laevis cDNA clone MGC:8218...    50   7e-05
(Q4V8Q1) RecName: Full=Calpain 11;          EC=3.4.22.-; AltName...    49   9e-05
AY639153_1(AY639153|pid:none) Gecarcinus lateralis calpain B (Ca...    49   1e-04
(P34308) RecName: Full=Calpain clp-1;          EC=3.4.22.-;  &L2...    48   3e-04
BC169895_1(BC169895|pid:none) Xenopus laevis calpain, mRNA (cDNA...    47   3e-04
BC169897_1(BC169897|pid:none) Xenopus laevis calpain, mRNA (cDNA...    47   3e-04
AB016726_1(AB016726|pid:none) Schistosoma japonicum mRNA for cal...    47   5e-04
AY808568_1(AY808568|pid:none) Schistosoma japonicum SJCHGC05999 ...    47   5e-04

 psg: 0.89  gvh: 0.48  alm: 0.44  top: 0.30  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SSC516 is cyt

>SSC516F.Seq
AACTGAATCCTTCAAATTCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGGTAC
ATACATTGTCATTCCATCAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAACACT
TTTCACCGATGATAAGAATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGTTGA
ACAAGTTAAAGGTACTTGGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAACTTT
CTTCAATAATCCACAATTCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCATCGC
CGTTCAATTGATTCAAGATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTTATC
TCGTGATTCTCATTCTGAACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTTTTCAC
TAAAACTTCAAATTGGGAAAAGAGAAATGATATCGTTTGTCGTCTTCATGTTAAACCAGA
ATCACCAAGAGAATTCACTATCATTCCTTCAACTTTTGATCCAAGTGTTAATCGTTCATT
CAAACTTCAAGTTATTCTGATGTTTCAATTTCATTAGATGAAATTGAACAAAAAGATGAA
TCCTCTGATTCTGAACAAAATGATGATTA----------