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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK718
Library AF
(Link to library)
Clone ID AFK718
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13973-1
Original site URL
Representative seq. ID AFK718Z
(Link to Original site)
Representative DNA sequence 
>AFK718 (AFK718Q) /CSM/AF/AFK7-A/AFK718Q.Seq.d/
XXXXXXXXXXATAAATATTGGATTTATAAATATCAGCATATATACGCCCCCATTTTTGTA
TGGTCTATATTCGATTAAATATAGATTATGTGATTATAGTGTTTTCACAGAGGGTTCAAT
TGGAAGGGTTAGAACTGCAAATGCTTCAAATTTTGAAATCATCTCTTTTATCATTGGTAA
ATTGGTTTTCATTGTTTTTAGATTCATCATACCATTACAATATCATAGTTTAGTAAACTT
GTTAACTTATTTCTTTATCGCTGAATTCTTTTTTGGTCTTTATTTATCCTTTGGTTTCCA
AGTTAGTCATTCTGCTGATAATTTAAAAATTGTTGCAACAAGTGTAAATGAAAATGATGA
ACCAACAAATGTTGATGAAGATTGGGCAATTCATCAAATTAAAACTACTCAAGATTATGG
TATTAATTCATATATGTGTTTATTTTTTTCGGGTGGTGTAAATTTACAAGTTGTTCATCA
CTTGTTTCCATCAATTTCACAAGAATACTATGGTGAATTAGTACCAATTATTAAAAAGGT
TTGTGATGAGTATGATGTACATTATAATATTCAACCAACATTTTATGCTGCTTTTAAATC
TCATATTGATTTTCTTTACAATATGGGTAATAATGAAAATTATGTAAGAAAATCTGTAAC
CGACTAGTAATTGAACCTAAAAATAAAAAAATAAAAAAATAAAAAATCCAACAAACCCAA
AAACAAAAATAAAAATATCGTTGCAACT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---NIGFINISIYTPPFLYGLYSIKYRLCDYSVFTEGSIGRVRTANASNFEIISFIIGKL
VFIVFRFIIPLQYHSLVNLLTYFFIAEFFFGLYLSFGFQVSHSADNLKIVATSVNENDEP
TNVDEDWAIHQIKTTQDYGINSYMCLFFSGGVNLQVVHHLFPSISQEYYGELVPIIKKVC
DEYDVHYNIQPTFYAAFKSHIDFLYNMGNNENYVRKSVTD**lnlkikk*knkksnkpkn
knknivat
Translated Amino Acid sequence (All Frames) 
Frame A:
---*ildl*isayirphfcmvyirlnidyviivfsqrvqleglelqmlqilkssllslvn
wfslfldssyhyniiv**tc*lislslnsflvfiyplvsklvillii*kllqqv*mkmmn
qqmlmkigqfiklkllkimvlihicvyffrvv*iyklfitcfhqfhkntmvn*yqllkrf
vmsmmyiiifnqhfmlllnlilifftiwvimkim*enl*ptsn*t*k*knkkiknptnpk
tkikislq


Frame B:
---kywiykyqhiyapifvwsifd*i*im*l*cfhrgfnwkg*nckcfkf*nhlfyhw*i
gfhcf*ihhtitis*fsklvnlflyr*ilfwslfilwfps*sfc**fknccnkck*k**t
nkc**rlgnssn*nysrlwy*fiyvfiffgwckftscsslvsinftrilw*istny*kgl
**v*ctl*ystnilccf*isy*fslqyg***klckkicnrlviepknkkikk*kiqqtqk
qk*kyrcn


Frame C:
---NIGFINISIYTPPFLYGLYSIKYRLCDYSVFTEGSIGRVRTANASNFEIISFIIGKL
VFIVFRFIIPLQYHSLVNLLTYFFIAEFFFGLYLSFGFQVSHSADNLKIVATSVNENDEP
TNVDEDWAIHQIKTTQDYGINSYMCLFFSGGVNLQVVHHLFPSISQEYYGELVPIIKKVC
DEYDVHYNIQPTFYAAFKSHIDFLYNMGNNENYVRKSVTD**lnlkikk*knkksnkpkn
knknivat
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK718 (AFK718Q) /CSM/AF/AFK7-A/AFK718Q.Seq.d/                       1283   0.0
VHC494 (VHC494Q) /CSM/VH/VHC4-D/VHC494Q.Seq.d/                       1209   0.0
CHB310 (CHB310Q) /CSM/CH/CHB3-A/CHB310Q.Seq.d/                       1104   0.0
VFM167 (VFM167Q) /CSM/VF/VFM1-C/VFM167Q.Seq.d/                        731   0.0
SFJ777 (SFJ777Q) /CSM/SF/SFJ7-D/SFJ777Q.Seq.d/                        571   e-162
VSH813 (VSH813Q) /CSM/VS/VSH8-A/VSH813Q.Seq.d/                         70   5e-11
VSF803 (VSF803Q) /CSM/VS/VSF8-A/VSF803Q.Seq.d/                         70   5e-11
VSF218 (VSF218Q) /CSM/VS/VSF2-A/VSF218Q.Seq.d/                         70   5e-11
VSC833 (VSC833Q) /CSM/VS/VSC8-B/VSC833Q.Seq.d/                         70   5e-11
VFO589 (VFO589Q) /CSM/VF/VFO5-D/VFO589Q.Seq.d/                         70   5e-11
own update 2002. 9.17
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB022097|AB022097.1 Dictyostelium discoideum FadB mRNA for delta 5 fatty acid desaturase, complete cds.    68   2e-17  3
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.    70   9e-17  4
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.    70   9e-17  4
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.    70   9e-17  4
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.    70   2e-13  4
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           34   7e-05  25
AL929354|AL929354.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 4/4.    40   2e-04  12
CR382400|CR382400.1 Plasmodium falciparum chromosome 6, complete sequence; segment 3/5.    36   0.002  15
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    36   0.002  13
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    36   0.005  12
dna update 2004. 8. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   423   e-121
(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   238   2e-63
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   219   4e-58
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   133   6e-30
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   132   1e-29
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   124   4e-28
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   120   6e-26
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   117   4e-25
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    115   9e-25
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   112   9e-24
protein update 2009. 4.24
PSORT 

 psg: 0.89  gvh: 0.51  alm: 0.23  top: 0.63  tms: 0.07  mit: 0.30  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFK718 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFK718Z
3' end seq. 
>AFK718Z.Seq
----------ATAAATATTGGATTTATAAATATCAGCATATATACGCCCCCATTTTTGTA
TGGTCTATATTCGATTAAATATAGATTATGTGATTATAGTGTTTTCACAGAGGGTTCAAT
TGGAAGGGTTAGAACTGCAAATGCTTCAAATTTTGAAATCATCTCTTTTATCATTGGTAA
ATTGGTTTTCATTGTTTTTAGATTCATCATACCATTACAATATCATAGTTTAGTAAACTT
GTTAACTTATTTCTTTATCGCTGAATTCTTTTTTGGTCTTTATTTATCCTTTGGTTTCCA
AGTTAGTCATTCTGCTGATAATTTAAAAATTGTTGCAACAAGTGTAAATGAAAATGATGA
ACCAACAAATGTTGATGAAGATTGGGCAATTCATCAAATTAAAACTACTCAAGATTATGG
TATTAATTCATATATGTGTTTATTTTTTTCGGGTGGTGTAAATTTACAAGTTGTTCATCA
CTTGTTTCCATCAATTTCACAAGAATACTATGGTGAATTAGTACCAATTATTAAAAAGGT
TTGTGATGAGTATGATGTACATTATAATATTCAACCAACATTTTATGCTGCTTTTAAATC
TCATATTGATTTTCTTTACAATATGGGTAATAATGAAAATTATGTAAGAAAATCTGTAAC
CGACTAGTAATTGAACCTAAAAATAAAAAAATAAAAAAATAAAAAATCCAACAAACCCAA
AAACAAAAATAAAAATATCGTTGCAACT
Length of 3' end seq. 738
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -