>AFB306 (AFB306Q) /CSM/AF/AFB3-A/AFB306Q.Seq.d/
AAATAAATAAAAATAAAAAATAAATATTTTTTATAATTATAAAAGGGTATTAAATTTCAA
ATTCTTTTTCATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATA
TATATAAAAATATAAAAAAAAATAAANTGAGAATTTTATATTTAGCTTCTCTTTTATTTT
TAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTC
ATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGNGCATATTNACCATTTGTATCAT
GTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATT
TATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAAC
CACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTT
GTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCXX
XXXXXXXX

inknkk*iffiiikgy*ISNSFSFIQFCNYKKRNQIIIYIYKNIKKNKXRILYLASLLFL
ITLYLSPTFGWGGGRDCESHRSEYTCKSDRSXAYXPFVSCCGKKEFFCVNRDHRNCCDDL
SCAKNTRTGEIFEIWSSCKPHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPCYGT--
-

Frame A:
k*ikiknkyfl*l*kgikfqilfhsfnfviikkgik*lyiyiki*kkix*efyi*llfyf
*lhyiyhqllvgvvaeivkvivqnihvnqievxhixhlyhvvvrknfsvliviieivvmi
yhvlktrelvkslrfgqvvnhieilyhiihqiqphvnh*vvkqevwnvnglnhhhvmel-
--


Frame B:
nk*k*kinifynykrvlnfkfffihsil*l*kkesnnyiyi*kykkk*xenfifsfsfif
nyiisitnfwlgwwqrl*kssfriym*ir*klxixticimlw*eriflc*s*s*kll**f
imc*khenw*nl*dlvkl*tt*rfctisftkynhm*iirl*skrygm*mg*iitmlwn--
-


Frame C:
inknkk*iffiiikgy*ISNSFSFIQFCNYKKRNQIIIYIYKNIKKNKXRILYLASLLFL
ITLYLSPTFGWGGGRDCESHRSEYTCKSDRSXAYXPFVSCCGKKEFFCVNRDHRNCCDDL
SCAKNTRTGEIFEIWSSCKPHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPCYGT--
-

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSA149 (SSA149Q) /CSM/SS/SSA1-C/SSA149Q.Seq.d/                        767   0.0
SLG851 (SLG851Q) /CSM/SL/SLG8-C/SLG851Q.Seq.d/                        767   0.0
SLB538 (SLB538Q) /CSM/SL/SLB5-B/SLB538Q.Seq.d/                        767   0.0
SLA438 (SLA438Q) /CSM/SL/SLA4-B/SLA438Q.Seq.d/                        767   0.0
SLA432 (SLA432Q) /CSM/SL/SLA4-B/SLA432Q.Seq.d/                        767   0.0
SLA402 (SLA402Q) /CSM/SL/SLA4-A/SLA402Q.Seq.d/                        767   0.0
SHL109 (SHL109Q) /CSM/SH/SHL1-A/SHL109Q.Seq.d/                        767   0.0
SHJ527 (SHJ527Q) /CSM/SH/SHJ5-B/SHJ527Q.Seq.d/                        767   0.0
SHH249 (SHH249Q) /CSM/SH/SHH2-C/SHH249Q.Seq.d/                        767   0.0
SHF542 (SHF542Q) /CSM/SH/SHF5-B/SHF542Q.Seq.d/                        767   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.   599  0.0  3
CA522886|CA522886.1 KS12015A12 KS12 Capsicum annuum cDNA, mRNA sequence.    40  0.047  2
BX284625|BX284625.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEYP-55F12.    42  0.092  3
AZ208391|AZ208391.1 SP_0150_A1_B06_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=150 Col=11 Row=C, DNA sequence.    48  0.11  1
AC073320|AC073320.8 Homo sapiens clone RP11-306G20, complete sequence.    48  0.11  1
AL662785|AL662785.5 Mouse DNA sequence from clone RP23-186N7 on chromosome 11.    38  0.33  5
AZ682969|AZ682969.1 ENTGM50TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    34  0.41  3
AZ547546|AZ547546.1 ENTDJ08TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    34  0.45  3
BH146587|BH146587.1 ENTPW38TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    34  0.48  3
AC016287|AC016287.4 Homo sapiens clone RP11-21L17, WORKING DRAFT SEQUENCE, 12 unordered pieces.    36  0.63  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       278   8e-74
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   274   9e-73
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   103   4e-21
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    70   3e-11
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    49   8e-05
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    47   4e-04
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    40   0.029
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    40   0.029
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    38   0.14
AB255883_1(AB255883|pid:none) Leucophaea maderae VgR mRNA for vi...    38   0.19

 psg: 0.89  gvh: 0.77  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.07
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum

>> prediction for AFB306 is exc

>AFB306F.Seq
AAATAAATAAAAATAAAAAATAAATATTTTTTATAATTATAAAAGGGTATTAAATTTCAA
ATTCTTTTTCATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATA
TATATAAAAATATAAAAAAAAATAAANTGAGAATTTTATATTTAGCTTCTCTTTTATTTT
TAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTC
ATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGNGCATATTNACCATTTGTATCAT
GTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATT
TATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAAC
CACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTT
GTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTC--
--------