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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK713
Library AF
(Link to library)
Clone ID AFK713
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16397-1
Original site URL
Representative seq. ID AFK713P
(Link to Original site)
Representative DNA sequence 
>AFK713 (AFK713Q) /CSM/AF/AFK7-A/AFK713Q.Seq.d/
TGTTTAATATATAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAG
TTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATT
GGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAG
TTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAG
ATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATT
TAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCA
AGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGNGAATCTGAATATTCCATTA
TGTTTGGNCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAG
GAAAGAACCATTTAATCAAGAAXXXXXXXXXXATNATAGTTTCCNGNTCTATACNTNTGG
TATNTATTATGAGNCANAATGTTCTCCANCTGAATTGNATCATGGTGTNTTAGNTGTTGG
TTATGGTGTTCAANGTANANATNATNAAGGNNCAGTATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fni*RKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSGKLV
HTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGSYIK
LLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIK---

---XSFXXYTXGXYYEXXCSPXELXHGVLXVGYGVQXXXXXGXV
Translated Amino Acid sequence (All Frames) 
Frame A:
cliykeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkmvnqvn*
filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkklivvvhts
syyhpn*inqhsmxnlniplclxqmfvvpqreftsfsttkerti*sr---

---xivsxsixxvximxxnvlxlnximvx*xlvmvfxvxxxkxqy

Frame B:
v*yikkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rrw*ir*is
sycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*lwwfihq
vitiqirsisirwxi*ifhyvwxrclwclkessrhsqlqrkepfnqe---

---x*fpxlyxwyxl*xxmfsx*ixswcxxcwlwcsxxxxxrxsi

Frame C:
fni*RKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSGKLV
HTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGSYIK
LLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIK---

---XSFXXYTXGXYYEXXCSPXELXHGVLXVGYGVQXXXXXGXV
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        831   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        831   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        831   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        831   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        831   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                        831   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        831   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        831   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        831   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        831   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   831   0.0    2
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.   131   1e-26  1
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.   131   1e-26  1
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.   131   1e-26  1
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   7e-21  2
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.    48   0.003  2
AW010688|AW010688.1 ST11A05 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST11A05, mRNA sequence.    46   0.017  2
BM158718|BM158718.1 NXLV_039_C04_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_039_C04 5', mRNA sequence.    46   0.018  2
BM158719|BM158719.1 NXLV_039_C05_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_039_C05 5', mRNA sequence.    46   0.018  2
CO175084|CO175084.1 NDL1_48_F11.b1_A029 Needles control Pinus taeda cDNA clone NDL1_48_F11_A029 3', mRNA sequence.    46   0.019  2
dna update 2004. 8. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   297   2e-79
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   192   8e-48
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   189   4e-47
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   189   4e-47
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   188   9e-47
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   187   2e-46
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   187   2e-46
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   186   4e-46
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   186   4e-46
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   186   4e-46
protein update 2009. 4.24
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.17  tms: 0.00  mit: 0.41  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear

>> prediction for AFK713 is exc
5' end seq. ID AFK713F
5' end seq. 
>AFK713F.Seq
TGTTTAATATATAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAG
TTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATT
GGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAG
TTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAG
ATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATT
TAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCA
AGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGNGAATCTGAATATTCCATTA
TGTTTGGNCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAG
GAAAGAACCATTTAATCAAGAA----------
Length of 5' end seq. 502
3' end seq. ID AFK713Z
3' end seq. 
>AFK713Z.Seq
----------ATNATAGTTTCCNGNTCTATACNTNTGGTATNTATTATGAGNCANAATGT
TCTCCANCTGAATTGNATCATGGTGTNTTAGNTGTTGGTTATGGTGTTCAANGTANANAT
NATNAAGGNNCAGTATT
Length of 3' end seq. 127
Connected seq. ID AFK713P
Connected seq. 
>AFK713P.Seq
TGTTTAATATATAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAG
TTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATT
GGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAG
TTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAG
ATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATT
TAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCA
AGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGNGAATCTGAATATTCCATTA
TGTTTGGNCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAG
GAAAGAACCATTTAATCAAGAA----------ATNATAGTTTCCNGNTCTATACNTNTGG
TATNTATTATGAGNCANAATGTTCTCCANCTGAATTGNATCATGGTGTNTTAGNTGTTGG
TTATGGTGTTCAANGTANANATNATNAAGGNNCAGTATT
Length of connected seq. 629
Full length Seq ID -
Full length Seq. -
Length of full length seq. -