>AFK689 (AFK689Q) /CSM/AF/AFK6-D/AFK689Q.Seq.d/
XXXXXXXXXXTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAG
TTGCCAATAAAGCTNGTTACAATTTCGATGAAAAAGAANCNTTNTCATTCGATNCCGTCT
CCAAATGGATNCAAGATGTCATTGGTGGTANAGTTTCTCCATTTGTTAAATCTCAACCAG
AACCAGAATCAAATGATGNTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGG
TTTTAGACTCACCAAAGNATGTTNTGGTTGAATTCTACGCTCCATGGTGCGGTCANNGCA
ANAATCTTGCTCCAATNTACNATAAATTAGGTGAANNCTTAAAAGACGTTGAATCTGTCT
CAATCGTAAAGATTGATGCCGACTCAAATNATGTACCAAGTGATATCGAAATTAGAGGTC
NCCCAACCATCATGCTCTTCAAAGCTGATGACAAAGANCCATCCACTCTCTTATGAAGGN
CAAAGAAATGATCATATGAACTNTGTTGAANTCATTCAANATAACGCTGCCA

---HMGLSGKVVPAISVDSVANKAXYNFDEKEXXSFDXVSKWXQDVIGGXVSPFVKSQPE
PESNDXPVKVAVGTTFKKLVLDSPKXVXVEFYAPWCGXXXNLAPXYXKLGEXLKDVESVS
IVKIDADSNXVPSDIEIRGXPTIMLFKADDKXPSTLL*rxkk*syelc*xhsx*rc

Frame A:
---HMGLSGKVVPAISVDSVANKAXYNFDEKEXXSFDXVSKWXQDVIGGXVSPFVKSQPE
PESNDXPVKVAVGTTFKKLVLDSPKXVXVEFYAPWCGXXXNLAPXYXKLGEXLKDVESVS
IVKIDADSNXVPSDIEIRGXPTIMLFKADDKXPSTLL*rxkk*syelc*xhsx*rc


Frame B:
---iwvslvklfqpslliqlpiklvtismkkxxxhsxpspngxkmslvvxflhllnlnqn
qnqmmxqlrlplvplskswf*thqxmxwlnstlhgavxaxillqxtin*vxx*ktlnlsq
s*rlmptqxmyqvisklevxqpscssklmtkxhplsyegqrndhmnxvexiqxnaa


Frame C:
---ygslw*scsshlc*fscq*sxlqfr*krxxxirxrlqmdxrchwwxsfsic*istrt
rik*xss*gcrwyhfqkvgfrltkxcxg*ilrsmvrsxqxscsnxx*ir*xlkrr*icln
rkd*crlkxctk*yrn*rspnhhalqs**qrxihslmkxkemii*txlxsfxitlp

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK689 (AFK689Q) /CSM/AF/AFK6-D/AFK689Q.Seq.d/                        892   0.0
VFN727 (VFN727Q) /CSM/VF/VFN7-B/VFN727Q.Seq.d/                        852   0.0
VFM432 (VFM432Q) /CSM/VF/VFM4-B/VFM432Q.Seq.d/                        852   0.0
VFL328 (VFL328Q) /CSM/VF/VFL3-B/VFL328Q.Seq.d/                        852   0.0
VFK816 (VFK816Q) /CSM/VF/VFK8-A/VFK816Q.Seq.d/                        852   0.0
VFK745 (VFK745Q) /CSM/VF/VFK7-B/VFK745Q.Seq.d/                        852   0.0
SFL445 (SFL445Q) /CSM/SF/SFL4-B/SFL445Q.Seq.d/                        852   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                        852   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                        852   0.0
SFJ691 (SFJ691Q) /CSM/SF/SFJ6-D/SFJ691Q.Seq.d/                        852   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   464   e-127  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    64   2e-06  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    56   6e-04  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52   0.009  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    52   0.009  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    52   0.009  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    52   0.009  1
CB175436|CB175436.1 pk71h09.y1 Ancylostoma ceylanicum L3 Ancylostoma ceylanicum cDNA 5' similar to TR:Q17770 Q17770 CODED FOR BY C. ELEGANS CDNA YK81H6.5. [1] ;, mRNA sequence.    52   0.009  1
CB189704|CB189704.1 pk84d07.y1 Ancylostoma ceylanicum L3 Ancylostoma ceylanicum cDNA 5' similar to TR:Q17770 Q17770 CODED FOR BY C. ELEGANS CDNA YK81H6.5. [1] ;, mRNA sequence.    52   0.009  1
CA341498|CA341498.1 pk21h01.x1 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 3' similar to TR:Q17770 Q17770 CODED FOR BY C. ELEGANS CDNA YK81H6.5. [1] ;, mRNA sequence.    52   0.009  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   119   6e-26
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   116   4e-25
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   115   7e-25
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   115   7e-25
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   112   7e-24
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   111   1e-23
BC064163_1(BC064163|pid:none) Xenopus tropicalis hypothetical pr...   110   2e-23
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   110   2e-23
AJ440747_1(AJ440747|pid:none) Ostertagia ostertagi mRNA for prot...   110   3e-23
BC067155_1(BC067155|pid:none) Danio rerio zgc:77086, mRNA (cDNA ...   108   8e-23

 psg: 0.79  gvh: 0.44  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFK689 is cyt

>AFK689Z.Seq
----------TCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAG
TTGCCAATAAAGCTNGTTACAATTTCGATGAAAAAGAANCNTTNTCATTCGATNCCGTCT
CCAAATGGATNCAAGATGTCATTGGTGGTANAGTTTCTCCATTTGTTAAATCTCAACCAG
AACCAGAATCAAATGATGNTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGG
TTTTAGACTCACCAAAGNATGTTNTGGTTGAATTCTACGCTCCATGGTGCGGTCANNGCA
ANAATCTTGCTCCAATNTACNATAAATTAGGTGAANNCTTAAAAGACGTTGAATCTGTCT
CAATCGTAAAGATTGATGCCGACTCAAATNATGTACCAAGTGATATCGAAATTAGAGGTC
NCCCAACCATCATGCTCTTCAAAGCTGATGACAAAGANCCATCCACTCTCTTATGAAGGN
CAAAGAAATGATCATATGAACTNTGTTGAANTCATTCAANATAACGCTGCCA