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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK655
Library AF
(Link to library)
Clone ID AFK655
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15018-1
Original site URL
Representative seq. ID AFK655P
(Link to Original site)
Representative DNA sequence 
>AFK655 (AFK655Q) /CSM/AF/AFK6-C/AFK655Q.Seq.d/
AATTTTTTGTTTTTTTATTTTTTTTAATTTTTATTTTTTATTTTACTATTTAGTAGTAAC
TTGTATTTGTTTAATTTAAAGTTGTAATCATATTATTTTTATAATCATTATTATTAATAC
TTAAAATATCATTATCTCTATATTTTTAAATTTATAAAACAAAAATGGAACAGAAAATGG
ATTTTTTAAATGTTAAACCAATCGAATTAGAGTTACAAGGTTTATCAATTGCAACTGTAA
AAAATAACGAACAATGGGATTTAGATGGATTTAATCAATATAAATTTGTAGATGAATCAG
AATATGAAGATCAACCATCATTTCAAAAGAATTTATCACACGATCTTACATTTTTACTTC
CAACATTAGATCAATTACAAGATGGTGCCGATTCAAGTGGACATGTTGATCAATCACATT
TAAGAGGTAGAATCTATGCACATAAAGAAAAGGTTGCATTGAAATCCGAATATTTTAGTC
TCCTCTTAACCAATGGAATGAAAGAGAGTAAACAACAAGAAATTTCAATTCCAAATATTT
CAGTCGACATTTTCTTTGAAATTTTAATGTTTTTATATACATCCGAGTTATCAGTAAATC
AAGAGAATTTAACAAGTATGTTTTTAGTTTGTGATCAATTTCTCTTGGAAGATGGTAAAC
AAATTTGTAGAGATTTCATTAAAACTTTAGATAATTTAATTTTAGAAAGATTTTTATTAG
AAGATAATGGTTTATGTTCAGAATTAACAGACCTTTATATAGAACAAGTTTGTTCAAATT
CATCATTTTTCTTAAATATTGAAAGAGTTTCAAAACTTGGAAAAGATGTATTGATCAAAG
TCTTGAATAGAGATGATATACCAATGAAAGAGATTGATATTTTTAGATCTACAATCGAGT
GGGTTTCTTGTAACACTGTATTATTATCATXXXXXXXXXXTAGTCATTTAATTCCTTCAA
ATTTATTATTAGAATCATATAGATTCCTTTCAAAACCAAAGTTAATACCAATGAATGAAA
ACGATTTACTCAATCCTAGATTAACACCAAGAAAGAAACCAAATCAAACTACATTGGTAC
CTGGTTGTGATGTTATATCATTCCAATGTCCTGATCATAAGAAATGTTATTCAGTATCAT
GGCCAATTCATAATTTTAGCGCAATAAAGAGTCAAAAACATGTTAGTAACTGTTTTGAAA
TGTATGGTTTAACTTGGAAAATGTGGGCTTACCCAGCTGGTGAAGCAAAGCACAGTGATA
GTTTTTCAGTTTATTTAGAAGCAGTTCGTGTAAAAGAGAAGGAATCTTACGAATTTTTAA
GAAATACAACATTCTTTTTCGGTCTTGTAAATCAAAAGAATAAAACACTTTGTCGTCACT
ATCCCTCATCTCCAAATGTTTTATTCAATTATGAGAAAAGTGTTTGGGGTAATGGTTTAA
TAGAATTAAAACTTTTGTATGATAAATCTTTAGGTTATATGGATAATGATTGTGTAACAA
TTCAATTACATATTTTAGAATGTATTGCATTGGAGGTTAAATTTAATTTTTTAATCAATA
ACAAATATTNACGCCATTAGATTTTTTTTAAAAAAGG
sequence update 2003. 5. 2
Translated Amino Acid sequence 
ffvflfflififyfti***lvfv*fkvviilfl*slllilkislslyf*IYKTKMEQKMD
FLNVKPIELELQGLSIATVKNNEQWDLDGFNQYKFVDESEYEDQPSFQKNLSHDLTFLLP
TLDQLQDGADSSGHVDQSHLRGRIYAHKEKVALKSEYFSLLLTNGMKESKQQEISIPNIS
VDIFFEILMFLYTSELSVNQENLTSMFLVCDQFLLEDGKQICRDFIKTLDNLILERFLLE
DNGLCSELTDLYIEQVCSNSSFFLNIERVSKLGKDVLIKVLNRDDIPMKEIDIFRSTIEW
VSCNTVLLS---

---SHLIPSNLLLESYRFLSKPKLIPMNENDLLNPRLTPRKKPNQTTLVPGCDVISFQCP
DHKKCYSVSWPIHNFSAIKSQKHVSNCFEMYGLTWKMWAYPAGEAKHSDSFSVYLEAVRV
KEKESYEFLRNTTFFFGLVNQKNKTLCRHYPSSPNVLFNYEKSVWGNGLIELKLLYDKSL
GYMDNDCVTIQLHILECIALEVKFNFLINNKYXRH*iffkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nflffyff*flffillfssnlylfnlkl*syyfynhyy*ylkyhylyifkfikqkwnrkw
if*mlnqsn*sykvyqlql*kitnngi*mdlininl*mnqnmkinhhfkriyhtilhfyf
qh*inykmvpiqvdmlinhi*evesmhikkrlh*npnilvss*pme*krvnnkkfqfqif
qstfslkf*cfyihpsyq*ikri*qvcf*fvinfswkmvnkfveislkl*ii*f*kdfy*
kimvyvqn*qtfi*nkfvqihhfs*ilkefqnlekmy*sks*iemiyq*krlifldlqss
gflvtlyyyh---

---*sfnsfkfiirii*ipfktkvntne*krftqs*intkketksnyigtwl*cyiipms
*s*emlfsimans*f*rnkesktc**lf*nvwfnlenvglpsw*skaq**ffslfrsssc
kregilrifkkynilfrsckske*ntlsslsliskcfiql*ekclg*wfnriktfv**if
rlyg**lcnnsityfrmycigg*i*ffnq*qixtpldff*kr

Frame B:
ifcffiffnfyflfyylvvtcicli*scnhiifiiiiint*niiisiflnl*nkngteng
ffkc*tnrirvtrfincnckk*rtmgfrwi*si*icr*iri*rstiiskefitrsyifts
nirsitrwcrfkwtc*sitfkr*nlct*rkgcieirif*splnqwnere*ttrnfnskyf
srhfl*nfnvfiyirvisksrefnkyvfsl*sislgrw*tnl*rfh*nfr*fnfrkifir
r*wfmfrinrplyrtslfkfiiflky*ksfktwkrcidqsle*r*ytnerd*yf*iynrv
gfl*hciii---

---SHLIPSNLLLESYRFLSKPKLIPMNENDLLNPRLTPRKKPNQTTLVPGCDVISFQCP
DHKKCYSVSWPIHNFSAIKSQKHVSNCFEMYGLTWKMWAYPAGEAKHSDSFSVYLEAVRV
KEKESYEFLRNTTFFFGLVNQKNKTLCRHYPSSPNVLFNYEKSVWGNGLIELKLLYDKSL
GYMDNDCVTIQLHILECIALEVKFNFLINNKYXRH*iffkk

Frame C:
ffvflfflififyfti***lvfv*fkvviilfl*slllilkislslyf*IYKTKMEQKMD
FLNVKPIELELQGLSIATVKNNEQWDLDGFNQYKFVDESEYEDQPSFQKNLSHDLTFLLP
TLDQLQDGADSSGHVDQSHLRGRIYAHKEKVALKSEYFSLLLTNGMKESKQQEISIPNIS
VDIFFEILMFLYTSELSVNQENLTSMFLVCDQFLLEDGKQICRDFIKTLDNLILERFLLE
DNGLCSELTDLYIEQVCSNSSFFLNIERVSKLGKDVLIKVLNRDDIPMKEIDIFRSTIEW
VSCNTVLLS---

---vi*flqiyy*nhidsfqnqs*yq*mktiysild*hqernqiklhwylvvmlyhsnvl
iirnviqyhgqfiilaq*rvknmlvtvlkcmv*lgkcgltqlvkqstvivfqfi*kqfv*
krrnltnf*eiqhsfsvl*ikrikhfvvtiphlqmfysimrkvfgvmv**n*nfcminl*
viwimiv*qfnyif*nvlhwrlnlif*sitnixairfflkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK655 (AFK655Q) /CSM/AF/AFK6-C/AFK655Q.Seq.d/                       2755   0.0
SLC789 (SLC789Q) /CSM/SL/SLC7-D/SLC789Q.Seq.d/                       1219   0.0
SSF141 (SSF141Q) /CSM/SS/SSF1-B/SSF141Q.Seq.d/                       1158   0.0
VFN519 (VFN519Q) /CSM/VF/VFN5-A/VFN519Q.Seq.d/                         42   0.026
VFN234 (VFN234Q) /CSM/VF/VFN2-B/VFN234Q.Seq.d/                         42   0.026
SSH453 (SSH453Q) /CSM/SS/SSH4-C/SSH453Q.Seq.d/                         42   0.026
CFC633 (CFC633Q) /CSM/CF/CFC6-B/CFC633Q.Seq.d/                         42   0.026
CFC594 (CFC594Q) /CSM/CF/CFC5-D/CFC594Q.Seq.d/                         42   0.026
AFO796 (AFO796Q) /CSM/AF/AFO7-D/AFO796Q.Seq.d/                         42   0.026
AFK214 (AFK214Q) /CSM/AF/AFK2-A/AFK214Q.Seq.d/                         42   0.026
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    38   1e-09  16
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   1e-08  23
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    36   1e-07  22
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    34   3e-07  22
BD061520|BD061520.1 Genome DNA of symbiotic bacteria of aphid.     36   4e-07  29
AR409405|AR409405.1 Sequence 1 from patent US 6632935.             36   4e-07  29
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    36   7e-07  30
AL023828|AL023828.1 Caenorhabditis elegans YAC Y17G7B.             34   2e-05  15
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   2e-05  22
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    40   2e-05  22
dna update 2004. 5.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5U504) RecName: Full=Kelch repeat and BTB domain-containing pr...    72   5e-11
DQ327670_1(DQ327670|pid:none) Xenopus laevis BTB domain protein ...    70   3e-10
BC087456_1(BC087456|pid:none) Xenopus laevis hypothetical LOC496...    69   3e-10
(Q5EB39) RecName: Full=Kelch repeat and BTB domain-containing pr...    69   6e-10
(Q6DFF7) RecName: Full=Ectoderm-neural cortex protein 2; AltName...    69   6e-10
(Q9Y573) RecName: Full=Actin-binding protein IPP; AltName: Full=...    67   1e-09
BC032544_1(BC032544|pid:none) Homo sapiens intracisternal A part...    67   1e-09
BC120053_1(BC120053|pid:none) Bos taurus kelch-like 7 (Drosophil...    67   2e-09
AY028802_1(AY028802|pid:none) Homo sapiens kelch/BTB protein mRN...    67   2e-09
AK299006_1(AK299006|pid:none) Homo sapiens cDNA FLJ53164 complet...    67   2e-09
protein update 2009. 3.28
PSORT 

 psg: 0.65  gvh: 0.30  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for AFK655 is cyt
5' end seq. ID AFK655F
5' end seq. 
>AFK655F.Seq
AATTTTTTGTTTTTTTATTTTTTTTAATTTTTATTTTTTATTTTACTATTTAGTAGTAAC
TTGTATTTGTTTAATTTAAAGTTGTAATCATATTATTTTTATAATCATTATTATTAATAC
TTAAAATATCATTATCTCTATATTTTTAAATTTATAAAACAAAAATGGAACAGAAAATGG
ATTTTTTAAATGTTAAACCAATCGAATTAGAGTTACAAGGTTTATCAATTGCAACTGTAA
AAAATAACGAACAATGGGATTTAGATGGATTTAATCAATATAAATTTGTAGATGAATCAG
AATATGAAGATCAACCATCATTTCAAAAGAATTTATCACACGATCTTACATTTTTACTTC
CAACATTAGATCAATTACAAGATGGTGCCGATTCAAGTGGACATGTTGATCAATCACATT
TAAGAGGTAGAATCTATGCACATAAAGAAAAGGTTGCATTGAAATCCGAATATTTTAGTC
TCCTCTTAACCAATGGAATGAAAGAGAGTAAACAACAAGAAATTTCAATTCCAAATATTT
CAGTCGACATTTTCTTTGAAATTTTAATGTTTTTATATACATCCGAGTTATCAGTAAATC
AAGAGAATTTAACAAGTATGTTTTTAGTTTGTGATCAATTTCTCTTGGAAGATGGTAAAC
AAATTTGTAGAGATTTCATTAAAACTTTAGATAATTTAATTTTAGAAAGATTTTTATTAG
AAGATAATGGTTTATGTTCAGAATTAACAGACCTTTATATAGAACAAGTTTGTTCAAATT
CATCATTTTTCTTAAATATTGAAAGAGTTTCAAAACTTGGAAAAGATGTATTGATCAAAG
TCTTGAATAGAGATGATATACCAATGAAAGAGATTGATATTTTTAGATCTACAATCGAGT
GGGTTTCTTGTAACACTGTATTATTATCATNNNNNNNNNN
Length of 5' end seq. 940
3' end seq. ID AFK655Z
3' end seq. 
>AFK655Z.Seq
----------TAGTCATTTAATTCCTTCAAATTTATTATTAGAATCATATAGATTCCTTT
CAAAACCAAAGTTAATACCAATGAATGAAAACGATTTACTCAATCCTAGATTAACACCAA
GAAAGAAACCAAATCAAACTACATTGGTACCTGGTTGTGATGTTATATCATTCCAATGTC
CTGATCATAAGAAATGTTATTCAGTATCATGGCCAATTCATAATTTTAGCGCAATAAAGA
GTCAAAAACATGTTAGTAACTGTTTTGAAATGTATGGTTTAACTTGGAAAATGTGGGCTT
ACCCAGCTGGTGAAGCAAAGCACAGTGATAGTTTTTCAGTTTATTTAGAAGCAGTTCGTG
TAAAAGAGAAGGAATCTTACGAATTTTTAAGAAATACAACATTCTTTTTCGGTCTTGTAA
ATCAAAAGAATAAAACACTTTGTCGTCACTATCCCTCATCTCCAAATGTTTTATTCAATT
ATGAGAAAAGTGTTTGGGGTAATGGTTTAATAGAATTAAAACTTTTGTATGATAAATCTT
TAGGTTATATGGATAATGATTGTGTAACAATTCAATTACATATTTTAGAATGTATTGCAT
TGGAGGTTAAATTTAATTTTTTAATCAATAACAAATATTNACGCCATTAGATTTTTTTTA
AAAAAGG
Length of 3' end seq. 657
Connected seq. ID AFK655P
Connected seq. 
>AFK655P.Seq
AATTTTTTGTTTTTTTATTTTTTTTAATTTTTATTTTTTATTTTACTATTTAGTAGTAAC
TTGTATTTGTTTAATTTAAAGTTGTAATCATATTATTTTTATAATCATTATTATTAATAC
TTAAAATATCATTATCTCTATATTTTTAAATTTATAAAACAAAAATGGAACAGAAAATGG
ATTTTTTAAATGTTAAACCAATCGAATTAGAGTTACAAGGTTTATCAATTGCAACTGTAA
AAAATAACGAACAATGGGATTTAGATGGATTTAATCAATATAAATTTGTAGATGAATCAG
AATATGAAGATCAACCATCATTTCAAAAGAATTTATCACACGATCTTACATTTTTACTTC
CAACATTAGATCAATTACAAGATGGTGCCGATTCAAGTGGACATGTTGATCAATCACATT
TAAGAGGTAGAATCTATGCACATAAAGAAAAGGTTGCATTGAAATCCGAATATTTTAGTC
TCCTCTTAACCAATGGAATGAAAGAGAGTAAACAACAAGAAATTTCAATTCCAAATATTT
CAGTCGACATTTTCTTTGAAATTTTAATGTTTTTATATACATCCGAGTTATCAGTAAATC
AAGAGAATTTAACAAGTATGTTTTTAGTTTGTGATCAATTTCTCTTGGAAGATGGTAAAC
AAATTTGTAGAGATTTCATTAAAACTTTAGATAATTTAATTTTAGAAAGATTTTTATTAG
AAGATAATGGTTTATGTTCAGAATTAACAGACCTTTATATAGAACAAGTTTGTTCAAATT
CATCATTTTTCTTAAATATTGAAAGAGTTTCAAAACTTGGAAAAGATGTATTGATCAAAG
TCTTGAATAGAGATGATATACCAATGAAAGAGATTGATATTTTTAGATCTACAATCGAGT
GGGTTTCTTGTAACACTGTATTATTATCAT----------TAGTCATTTAATTCCTTCAA
ATTTATTATTAGAATCATATAGATTCCTTTCAAAACCAAAGTTAATACCAATGAATGAAA
ACGATTTACTCAATCCTAGATTAACACCAAGAAAGAAACCAAATCAAACTACATTGGTAC
CTGGTTGTGATGTTATATCATTCCAATGTCCTGATCATAAGAAATGTTATTCAGTATCAT
GGCCAATTCATAATTTTAGCGCAATAAAGAGTCAAAAACATGTTAGTAACTGTTTTGAAA
TGTATGGTTTAACTTGGAAAATGTGGGCTTACCCAGCTGGTGAAGCAAAGCACAGTGATA
GTTTTTCAGTTTATTTAGAAGCAGTTCGTGTAAAAGAGAAGGAATCTTACGAATTTTTAA
GAAATACAACATTCTTTTTCGGTCTTGTAAATCAAAAGAATAAAACACTTTGTCGTCACT
ATCCCTCATCTCCAAATGTTTTATTCAATTATGAGAAAAGTGTTTGGGGTAATGGTTTAA
TAGAATTAAAACTTTTGTATGATAAATCTTTAGGTTATATGGATAATGATTGTGTAACAA
TTCAATTACATATTTTAGAATGTATTGCATTGGAGGTTAAATTTAATTTTTTAATCAATA
ACAAATATTNACGCCATTAGATTTTTTTTAAAAAAGG
Length of connected seq. 1587
Full length Seq ID -
Full length Seq. -
Length of full length seq. -