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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSA104
Library SS
(Link to library)
Clone ID SSA104
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15035-1
Original site URL
Representative seq. ID SSA104E
(Link to Original site)
Representative DNA sequence 
>SSA104 (SSA104Q) /CSM/SS/SSA1-A/SSA104Q.Seq.d/
CAAAAATGATAAAAACTATTTTAAAAAACTCTTACATCCAAAAAGAAATTACTACACACT
CAGCAACAGTTATTTTCAGTCATGGCTTAGGTGATAGTGGTGCTGGTTGGATTGAAGTAA
TGGAAGAGATTCAAAGTAGAAACAATGGTCACATTAGATTCATTTGTCCAAATGCACCAA
TTCAAGCAGTTACACTTAATGGTGGATTTAAAATGCCATCATGGTATGATATTAAATCAT
TATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATNTAATTGAAA
CTNTTNTCAAACATGAAATGGAAGAAGNGAAAATTCCAGCTGAAAGAATTATAATTGGTG
GTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAAAT
TAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAATT
CATTAAATAAAGAACAACCATTACTCATGATCCNCGGTGATTGTGATCAAGTAGTTCGTC
ATCAATGGGGTAAATTATCATTCGACCATTTAAAAAAGTCAAGGTATTTAATGGTGAATT
TTATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTGATTTAATGACAA
AGTTTATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
sequence update 1999. 1.25
Translated Amino Acid sequence 
KMIKTILKNSYIQKEITTHSATVIFSHGLGDSGAGWIEVMEEIQSRNNGHIRFICPNAPI
QAVTLNGGFKMPSWYDIKSLSSRGDEDPAQVDESKNXIETXXKHEMEEXKIPAERIIIGG
FSQGAALSLYTFYSQTETKLGGCIALSGYLPLATKFVANSLNKEQPLLMIXGDCDQVVRH
QWGKLSFDHLKKSRYLMVNFITLKGLGHHSSPEEIDLMTKFISKTLPK*k*kkkkkkkkk
kkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
qk**klf*ktltskkkllhtqqqlfsvma*vivvlvglk*wkrfkvetmvtldsfvqmhq
fkqlhlmvdlkchhgmilnhyhqevmkiqlklmnqrx*lklxsnmkwkkxkfqlkel*lv
vfhkvqhyhyihfihklkpn*vvvlhyqviyhwqlnl*qih*iknnhys*sxvivik*fv
ingvnyhsti*ksqgi*w*il*h*kv*viiqvqrkli**qslflkhyqnknkkkkkkkkk
kkkkkkkk


Frame B:
kndknyfkkllhpkrnyytlsnsyfqswlr**wcwld*sngrdsk*kqwsh*ihlskctn
sssyt*wwi*naimv*y*iiiikr**rssss**ikexn*nxxqt*ngrxenss*knynww
fftrcsiiiiyilftn*nqirwlyciirlftign*icskfik*rttithdpr*l*sssss
smg*iiirpfkkvkvfngefynikrfrssfksrgn*fndkvyf*nitkikikkkkkkkkk
kkkkkkk


Frame C:
KMIKTILKNSYIQKEITTHSATVIFSHGLGDSGAGWIEVMEEIQSRNNGHIRFICPNAPI
QAVTLNGGFKMPSWYDIKSLSSRGDEDPAQVDESKNXIETXXKHEMEEXKIPAERIIIGG
FSQGAALSLYTFYSQTETKLGGCIALSGYLPLATKFVANSLNKEQPLLMIXGDCDQVVRH
QWGKLSFDHLKKSRYLMVNFITLKGLGHHSSPEEIDLMTKFISKTLPK*k*kkkkkkkkk
kkkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSA104 (SSA104Q) /CSM/SS/SSA1-A/SSA104Q.Seq.d/                       1227   0.0
VSI690 (VSI690Q) /CSM/VS/VSI6-D/VSI690Q.Seq.d/                       1179   0.0
AHE104 (AHE104Q) /CSM/AH/AHE1-A/AHE104Q.Seq.d/                       1142   0.0
SSJ323 (SSJ323Q) /CSM/SS/SSJ3-A/SSJ323Q.Seq.d/                       1043   0.0
VSF154 (VSF154Q) /CSM/VS/VSF1-C/VSF154Q.Seq.d/                        803   0.0
FC-BJ08 (FC-BJ08Q) /CSM/FC/FC-BJ/FC-BJ08Q.Seq.d/                      557   e-158
VSJ183 (VSJ183Q) /CSM/VS/VSJ1-D/VSJ183Q.Seq.d/                         38   0.19
VHI768 (VHI768Q) /CSM/VH/VHI7-C/VHI768Q.Seq.d/                         38   0.19
SFJ480 (SFJ480Q) /CSM/SF/SFJ4-D/SFJ480Q.Seq.d/                         38   0.19
FC-AA12 (FC-AA12Q) /CSM/FC/FC-AA/FC-AA12Q.Seq.d/                       38   0.19
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C24609) Dictyostelium discoideum slug cDNA, clone SL-X004.      1108   0.0    1
(BJ350199) Dictyostelium discoideum cDNA clone:dda39g01, 3' ...  1017   0.0    5
(BJ332427) Dictyostelium discoideum cDNA clone:dda39g01, 5' ...  1017   0.0    1
(AU264788) Dictyostelium discoideum vegetative cDNA clone:VS...   640   0.0    4
(AU264789) Dictyostelium discoideum vegetative cDNA clone:VS...   640   0.0    4
(AU075917) Dictyostelium discoideum slug cDNA, clone SSA104.      615   0.0    4
(AU269090) Dictyostelium discoideum vegetative cDNA clone:VS...   583   0.0    4
(AU269089) Dictyostelium discoideum vegetative cDNA clone:VS...   716   0.0    1
(AU284743) Dictyostelium discoideum gamete cDNA clone:FC-BJ0...   357   e-154  5
(AU074028) Dictyostelium discoideum slug cDNA, clone SSJ323.      377   e-100  1
dna update 2009. 7.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54T49) RecName: Full=Acyl-protein thioesterase 1 homolog 1;   ...   426   e-118
(Q6CGL4) RecName: Full=Acyl-protein thioesterase 1;          EC=...   159   9e-38
CR761348_1(CR761348|pid:none) Xenopus tropicalis finished cDNA, ...   155   1e-36
T52511(T52511)related to lysophospholipase [imported] - Neurospo...   155   1e-36
BC073342_1(BC073342|pid:none) Xenopus laevis MGC80756 protein, m...   155   1e-36
BT048041_1(BT048041|pid:none) Salmo salar clone ssal-evd-522-113...   152   1e-35
(Q5RBR7) RecName: Full=Acyl-protein thioesterase 1;          EC=...   150   4e-35
AK296257_1(AK296257|pid:none) Homo sapiens cDNA FLJ60607 complet...   150   5e-35
(O75608) RecName: Full=Acyl-protein thioesterase 1;          EC=...   150   5e-35
(O77821) RecName: Full=Acyl-protein thioesterase 1;          EC=...   149   9e-35
protein update 2009. 7. 5
PSORT 

 psg: 0.55  gvh: 0.47  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for SSA104 is cyt
5' end seq. ID SSA104F
5' end seq. 
>SSA104F.Seq
CAAAAATGATAAAAACTATTTTAAAAAACTCTTACATCCAAAAAGAAATTACTACACACT
CAGCAACAGTTATTTTCAGTCATGGCTTAGGTGATAGTGGTGCTGGTTGGATTGAAGTAA
TGGAAGAGATTCAAAGTAGAAACAATGGTCACATTAGATTCATTTGTCCAAATGCACCAA
TTCAAGCAGTTACACTTAATGGTGGATTTAAAATGCCATCATGGTATGATATTAAATCAT
TATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATATAATTGAAA
CTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAGCTGAAAGAATTATAATTGGTG
GTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAAAT
TAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAATT
CATTAAATAAAGAACAACCATTACTCATGATCCACGGTGATTGTGATCAAGTAGTTCGTC
ATCAATGGGGTAAATTATCATTCGACCATTTAAAAAAGTCAAGGTATTTAATGGTGAATT
TTATAACATTAAAAGGTTTAGG----------
Length of 5' end seq. 622
3' end seq. ID SSA104Z
3' end seq. 
>SSA104Z.Seq
----------GGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATTTAATTGAAAC
TTTTTTCAAACATGAAATGGAAGAAGGGAAAATTCCAGCTGAAAGAATTATAATTGGTGG
TTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAAATT
AGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAATTC
ATTAAATAAAGAACAACCATTACTCATGATCCCCGGTGATTGTGATCAAGTAGTTCGTCA
TCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTCAAGGTATTAATGGTGAATTTAT
AACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTGATTTAATGACAAAGTT
TATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAA
Length of 3' end seq. 490
Connected seq. ID SSA104P
Connected seq. 
>SSA104P.Seq
CAAAAATGATAAAAACTATTTTAAAAAACTCTTACATCCAAAAAGAAATTACTACACACT
CAGCAACAGTTATTTTCAGTCATGGCTTAGGTGATAGTGGTGCTGGTTGGATTGAAGTAA
TGGAAGAGATTCAAAGTAGAAACAATGGTCACATTAGATTCATTTGTCCAAATGCACCAA
TTCAAGCAGTTACACTTAATGGTGGATTTAAAATGCCATCATGGTATGATATTAAATCAT
TATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATATAATTGAAA
CTATTATCAAACATGAAATGGAAGAAGAGAAAATTCCAGCTGAAAGAATTATAATTGGTG
GTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAAAT
TAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAATT
CATTAAATAAAGAACAACCATTACTCATGATCCACGGTGATTGTGATCAAGTAGTTCGTC
ATCAATGGGGTAAATTATCATTCGACCATTTAAAAAAGTCAAGGTATTTAATGGTGAATT
TTATAACATTAAAAGGTTTAGG----------GGTGATGAAGATCCAGCTCAAGTTGATG
AATCAAAGAATTTAATTGAAACTTTTTTCAAACATGAAATGGAAGAAGGGAAAATTCCAG
CTGAAAGAATTATAATTGGTGGTTTTTCACAAGGTGCAGCATTATCATTATATACATTTT
ATTCACAAACTGAAACCAAATTAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGG
CAACTAAATTTGTAGCAAATTCATTAAATAAAGAACAACCATTACTCATGATCCCCGGTG
ATTGTGATCAAGTAGTTCGTCATCAATGGGGTAAATTATCATTCGACCATTTAAAAAGTC
AAGGTATTAATGGTGAATTTATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGG
AAATTGATTTAATGACAAAGTTTATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Length of connected seq. 1112
Full length Seq ID SSA104E
Full length Seq. 
>SSA104E.Seq
CAAAAATGATAAAAACTATTTTAAAAAACTCTTACATCCAAAAAGAAATTACTACACACT
CAGCAACAGTTATTTTCAGTCATGGCTTAGGTGATAGTGGTGCTGGTTGGATTGAAGTAA
TGGAAGAGATTCAAAGTAGAAACAATGGTCACATTAGATTCATTTGTCCAAATGCACCAA
TTCAAGCAGTTACACTTAATGGTGGATTTAAAATGCCATCATGGTATGATATTAAATCAT
TATCATCAAGAGGTGATGAAGATCCAGCTCAAGTTGATGAATCAAAGAATNTAATTGAAA
CTNTTNTCAAACATGAAATGGAAGAAGNGAAAATTCCAGCTGAAAGAATTATAATTGGTG
GTTTTTCACAAGGTGCAGCATTATCATTATATACATTTTATTCACAAACTGAAACCAAAT
TAGGTGGTTGTATTGCATTATCAGGTTATTTACCATTGGCAACTAAATTTGTAGCAAATT
CATTAAATAAAGAACAACCATTACTCATGATCCNCGGTGATTGTGATCAAGTAGTTCGTC
ATCAATGGGGTAAATTATCATTCGACCATTTAAAAAAGTCAAGGTATTTAATGGTGAATT
TTATAACATTAAAAGGTTTAGGTCATCATTCAAGTCCAGAGGAAATTGATTTAATGACAA
AGTTTATTTCTAAAACATTACCAAAATAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
Length of full length seq. 744