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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLJ254
Library SL
(Link to library)
Clone ID SLJ254
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLJ254P
(Link to Original site)
Representative DNA sequence 
>SLJ254 (SLJ254Q) /CSM/SL/SLJ2-C/SLJ254Q.Seq.d/
CGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTT
TGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACAC
TGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAA
GAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGA
TATGGAAAAAATCTGGCATCAXXXXXXXXXXCTCCACCAGAACGTAAATACTCTGTCTGG
ATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAA
GAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACT
AGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATA
TAATTATTTTTAATGGTTGTTGATCTTTATCCGACTATCAAAAAAAAAAAAAA
sequence update 1999. 3. 5
Translated Amino Acid sequence 
GEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSK
RGILTLKYPIEHGIVTNWDDMEKIWH---

---PPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesasht
nkyi*mynnnki*lflmvvdlyptikkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
r*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcygrygskrllrr**spik
erylnpqipn*trycyqlg*ygknlas---

---lhqnvntlsglvdls*lhsqlsnkcgspkknmtnqvhqlstenvskqlklvmkvllt
qtnifkciiiikynyf*wllifirlskkkk

Frame B:
GEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSK
RGILTLKYPIEHGIVTNWDDMEKIWH---

---strt*ilcldwwiylsftlnfptnvdlqrri*rirsincpqkmflnn*n***kcfsh
kqiylnv****niiifngc*slsdyqkkkk

Frame C:
vkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkktlt*vmkpnqr
evs*psntqlntvllptgmiwkksgi---

---PPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesasht
nkyi*mynnnki*lflmvvdlyptikkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO611 (VFO611Q) /CSM/VF/VFO6-A/VFO611Q.Seq.d/                        517   e-146
VFO483 (VFO483Q) /CSM/VF/VFO4-D/VFO483Q.Seq.d/                        517   e-146
VFO120 (VFO120Q) /CSM/VF/VFO1-A/VFO120Q.Seq.d/                        517   e-146
VFN701 (VFN701Q) /CSM/VF/VFN7-A/VFN701Q.Seq.d/                        517   e-146
VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                        517   e-146
VFM807 (VFM807Q) /CSM/VF/VFM8-A/VFM807Q.Seq.d/                        517   e-146
VFM725 (VFM725Q) /CSM/VF/VFM7-B/VFM725Q.Seq.d/                        517   e-146
VFM720 (VFM720Q) /CSM/VF/VFM7-A/VFM720Q.Seq.d/                        517   e-146
VFM705 (VFM705Q) /CSM/VF/VFM7-A/VFM705Q.Seq.d/                        517   e-146
VFM609 (VFM609Q) /CSM/VF/VFM6-A/VFM609Q.Seq.d/                        517   e-146
own update 2001.11.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   517  0.0  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   436  0.0  4
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      486  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        452  0.0  4
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   452  0.0  4
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   414  e-170  6
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   389  e-168  4
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   406  e-163  2
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     365  e-155  2
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   315  e-133  2
dna update 2003. 7.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A28258(A28258)actin 5C - fruit fly (Drosophila melanogaster)          163   2e-61
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   183   3e-45
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   183   3e-45
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   183   3e-45
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   183   3e-45
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   180   2e-44
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   179   4e-44
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   179   4e-44
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   178   6e-44
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        178   6e-44
protein update 2009. 6. 6
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SLJ254 is cyt
5' end seq. ID SLJ254F
5' end seq. 
>SLJ254F.Seq
CGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTT
TGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACAC
TGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAA
GAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGA
TATGGAAAAAATCTGGCATCA----------
Length of 5' end seq. 261
3' end seq. ID SLJ254Z
3' end seq. 
>SLJ254Z.Seq
----------CTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCT
TCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCA
TCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCAC
ACAAACAAATATATTTAAATGTATAATAATAATAAAATATAATTATTTTTAATGGTTGTT
GATCTTTATCCGACTATCAAAAAAAAAAAAAA
Length of 3' end seq. 262
Connected seq. ID SLJ254P
Connected seq. 
>SLJ254P.Seq
CGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTT
TGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACAC
TGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAA
GAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGA
TATGGAAAAAATCTGGCATCA----------CTCCACCAGAACGTAAATACTCTGTCTGG
ATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAA
GAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACT
AGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATA
TAATTATTTTTAATGGTTGTTGATCTTTATCCGACTATCAAAAAAAAAAAAAA
Length of connected seq. 523
Full length Seq ID -
Full length Seq. -
Length of full length seq. -