>SLJ230 (SLJ230Q) /CSM/SL/SLJ2-B/SLJ230Q.Seq.d/
XXXXXXXXXXGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGA
TTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCG
TATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCCCC
ACCAGAACGTAAATACTCTGTTTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTT
CCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAG
AAAATGTTTCTAAACAATTAATAAATATGTTGTATTTTATTTAAACTTATATTAAAAAAA
AAAA

---TTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPP
ERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tinkyvvfylnly*kkk

Frame A:
---kppttps*nvmlisvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klkslph
qnvntlfglvdls*lhsqlsnkcgspkknmtnqvhqlstenvskqlinmlyfi*tyikkk


Frame B:
---nhlqlhhem*c*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhcpt
rt*ilcldwwiylsftlnfptnvdlqrri*rirsincpqkmflnn**iccilfklilkkk


Frame C:
---TTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPP
ERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tinkyvvfylnly*kkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLK869 (SLK869Q) /CSM/SL/SLK8-C/SLK869Q.Seq.d/                        680   0.0
SLJ230 (SLJ230Q) /CSM/SL/SLJ2-B/SLJ230Q.Seq.d/                        680   0.0
SLI718 (SLI718Q) /CSM/SL/SLI7-A/SLI718Q.Seq.d/                        680   0.0
SLI675 (SLI675Q) /CSM/SL/SLI6-D/SLI675Q.Seq.d/                        680   0.0
SLI477 (SLI477Q) /CSM/SL/SLI4-D/SLI477Q.Seq.d/                        680   0.0
SLH419 (SLH419Q) /CSM/SL/SLH4-A/SLH419Q.Seq.d/                        680   0.0
SLH340 (SLH340Q) /CSM/SL/SLH3-B/SLH340Q.Seq.d/                        680   0.0
SLH102 (SLH102Q) /CSM/SL/SLH1-A/SLH102Q.Seq.d/                        680   0.0
SLF425 (SLF425Q) /CSM/SL/SLF4-B/SLF425Q.Seq.d/                        680   0.0
SLF423 (SLF423Q) /CSM/SL/SLF4-A/SLF423Q.Seq.d/                        680   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   585  e-160  3
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      569  e-159  1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   545  e-146  4
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        523  e-145  1
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   523  e-144  2
K02960|K02960.1 Slime mold (D.discoideum) actin 8 gene, 3' end.   486  e-133  1
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   480  e-130  5
AX771242|AX771242.1 Sequence 5 from Patent WO03034275.           454  e-124  1
J01278|J01278.1 Slime mold (D.discoideum) actin mRNA, coding region 3' end.   438  e-119  1
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     418  e-113  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   206   3e-52
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   206   3e-52
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   206   3e-52
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        206   3e-52
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   203   1e-51
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   203   1e-51
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   202   2e-51
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   201   8e-51
AY615868_1(AY615868|pid:none) Amastigomonas marina actin gene, p...   201   8e-51
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   201   8e-51

 psg: 0.43  gvh: 0.47  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SLJ230 is cyt

>SLJ230Z.Seq
----------GAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGA
TTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCG
TATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCCCC
ACCAGAACGTAAATACTCTGTTTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTT
CCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAG
AAAATGTTTCTAAACAATTAATAAATATGTTGTATTTTATTTAAACTTATATTAAAAAAA
AAAA