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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLJ194
Library SL
(Link to library)
Clone ID SLJ194
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLJ194Z
(Link to Original site)
Representative DNA sequence 
>SLJ194 (SLJ194Q) /CSM/SL/SLJ1-D/SLJ194Q.Seq.d/
XXXXXXXXXXGCTTTAGCACCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACG
TAAATACTCTGTCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAAAT
GTGGATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGTTT
CTAAATCATGATGAAAGTGCTTCACATAAAAATAATAATAATAATATAACAATAATAATA
TTTAAATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCGAC
CTTAAAAAAAAAAAAATAAAACC
sequence update 1998.10.22
Translated Amino Acid sequence 
---LAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*
immkvlhikiiiii*q**ylnv**nlitfflmvvdlyptlkkkk*n
Translated Amino Acid sequence (All Frames) 
Frame A:
---l*hhqp*klksllhqnvntlsglvdlf*lhsqlsnkcgsqkknmmnlvhqlstenvs
ks**kcft*k****ynnnni*mynki*llff*wllifirp*kkknkt


Frame B:
---fstinhen*nhcstrt*ilcldwwiyfsftlnfptnvdlkrri**iwsincpqkmfl
nhdesashknnnnnitiiifkciikfnyfffngc*slsdlkkkkik


Frame C:
---LAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*
immkvlhikiiiii*q**ylnv**nlitfflmvvdlyptlkkkk*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK467 (VSK467Q) /CSM/VS/VSK4-C/VSK467Q.Seq.d/                        387   e-107
SSM288 (SSM288Q) /CSM/SS/SSM2-D/SSM288Q.Seq.d/                        387   e-107
SSK713 (SSK713Q) /CSM/SS/SSK7-A/SSK713Q.Seq.d/                        387   e-107
SSH731 (SSH731Q) /CSM/SS/SSH7-B/SSH731Q.Seq.d/                        387   e-107
SLK555 (SLK555Q) /CSM/SL/SLK5-C/SLK555Q.Seq.d/                        387   e-107
SLK176 (SLK176Q) /CSM/SL/SLK1-D/SLK176Q.Seq.d/                        387   e-107
SLJ854 (SLJ854Q) /CSM/SL/SLJ8-C/SLJ854Q.Seq.d/                        387   e-107
SLJ257 (SLJ257Q) /CSM/SL/SLJ2-C/SLJ257Q.Seq.d/                        387   e-107
SLJ194 (SLJ194Q) /CSM/SL/SLJ1-D/SLJ194Q.Seq.d/                        387   e-107
SLI574 (SLI574Q) /CSM/SL/SLI5-D/SLI574Q.Seq.d/                        387   e-107
own update 2002.12.11
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        387  e-112  2
AX771242|AX771242.1 Sequence 5 from Patent WO03034275.           387  e-112  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   387  e-110  2
J01278|J01278.1 Slime mold (D.discoideum) actin mRNA, coding region 3' end.   379  e-110  2
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   333  8e-88  5
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   307  4e-84  3
K02954|K02954.1 Slime mold (D.discoideum) actin 2-sub 1 gene, 3' end.   287  1e-81  3
K02961|K02961.1 Slime mold (D.discoideum) actin mRNA ITL-1, 3' end.   274  6e-78  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   293  4e-77  5
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   287  4e-76  3
dna update 2003. 7.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   119   3e-26
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   119   3e-26
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   119   3e-26
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   119   3e-26
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   119   3e-26
J01278_1(J01278|pid:none) Slime mold (D.discoideum) actin mRNA, ...   119   3e-26
K02960_1(K02960|pid:none) Dictyostelium discoideum actin 8 gene,...   119   3e-26
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   119   3e-26
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   119   3e-26
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        119   3e-26
protein update 2009. 6. 6
PSORT 

 psg: 0.53  gvh: 0.49  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SLJ194 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLJ194Z
3' end seq. 
>SLJ194Z.Seq
----------GCTTTAGCACCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACG
TAAATACTCTGTCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAAAT
GTGGATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGTTT
CTAAATCATGATGAAAGTGCTTCACATAAAAATAATAATAATAATATAACAATAATAATA
TTTAAATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCGAC
CTTAAAAAAAAAAAAATAAAACC
Length of 3' end seq. 313
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -