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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLJ186
Library SL
(Link to library)
Clone ID SLJ186
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11764-1
Original site URL
Representative seq. ID SLJ186P
(Link to Original site)
Representative DNA sequence 
>SLJ186 (SLJ186Q) /CSM/SL/SLJ1-D/SLJ186Q.Seq.d/
AGGAAATCATTAACATTGCCAAACAAGTGAAAACACCATCCCTCACCATCTATTTGAAAC
CACATATGGCTCGTGATATGGATCGTGCAAAGATTGTAAAATCACAACTCGAATATACTA
CACTTGCAAATGTAACCTCTGCAACCGAAATCTACTATGATCCTGATCCACAAAATACCA
TCATCAGTGAAGATGCTGAATTTGTAAATAGTTATTTCGAAXXXXXXXXXXCACCAACCT
CACCATCTTATTCACCAACCTCACCATCATATTCACCATCATCCCCATCTTATTCACCAA
GTTCGCCATCATATTCACCAAGTTCACCATCATACTCACCATCATCACCAACCTTTACAA
ACAAATATAACTATCAACCAAATAATAAAAAGAAATAGATCAACATTAAGATACCATTTC
CTATTTTAAATATTTAATTTGTATATAAATAAATTTAAACTTAACAAATTAACAAAATAT
AAAAAAAAAAATAAACAA
sequence update 1999. 3. 5
Translated Amino Acid sequence 
EIINIAKQVKTPSLTIYLKPHMARDMDRAKIVKSQLEYTTLANVTSATEIYYDPDPQNTI
ISEDAEFVNSYFE---

---HQPHHLIHQPHHHIHHHPHLIHQVRHHIHQVHHHTHHHHQPLQTNITINQIIKRNRS
TLRYHFLF*ifnlyinkfklnkltkykkknkq
Translated Amino Acid sequence (All Frames) 
Frame A:
rksltlpnk*khhpspsi*nhiwlviwivqrl*nhnsnilhlqm*plqpkstmilihkip
ssvkmlnl*ivis---

---HQPHHLIHQPHHHIHHHPHLIHQVRHHIHQVHHHTHHHHQPLQTNITINQIIKRNRS
TLRYHFLF*ifnlyinkfklnkltkykkknkq

Frame B:
gnh*hcqtsentiphhlfettygs*ygsckdckittriyytckcnlcnrnll*s*stkyh
hq*rc*ick*lfr---

---tnltilftnltiiftiipilftkfaiiftkftiiltiitnlykqi*lstk**keidq
h*dtisyfkylici*inlnltn*qnikkkin

Frame C:
EIINIAKQVKTPSLTIYLKPHMARDMDRAKIVKSQLEYTTLANVTSATEIYYDPDPQNTI
ISEDAEFVNSYFE---

---ptspsysptspsyspsspsyspsspsyspsspsyspssptftnkynyqpnnkkk*in
ikipfpilni*fvyk*i*t*qinki*kkk*t
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLJ186 (SLJ186Q) /CSM/SL/SLJ1-D/SLJ186Q.Seq.d/                        892   0.0
CHH389 (CHH389Q) /CSM/CH/CHH3-D/CHH389Q.Seq.d/                        438   e-122
SLK713 (SLK713Q) /CSM/SL/SLK7-A/SLK713Q.Seq.d/                        398   e-110
AHN301 (AHN301Q) /CSM/AH/AHN3-A/AHN301Q.Seq.d/                        369   e-101
AHC236 (AHC236Q) /CSM/AH/AHC2-B/AHC236Q.Seq.d/                        345   4e-94
VHM557 (VHM557Q) /CSM/VH/VHM5-C/VHM557Q.Seq.d/                        301   5e-81
AHN332 (AHN332Q) /CSM/AH/AHN3-B/AHN332Q.Seq.d/                        224   1e-57
CHP723 (CHP723Q) /CSM/CH/CHP7-A/CHP723Q.Seq.d/                        200   1e-50
SLD707 (SLD707Q) /CSM/SL/SLD7-A/SLD707Q.Seq.d/                        103   3e-21
SHF261 (SHF261Q) /CSM/SH/SHF2-C/SHF261Q.Seq.d/                         40   0.031
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

S52651|S52651.1 RNA polymerase II largest subunit [Dictyostelium discoideum, mRNA Partial, 2874 nt].   432  0.0  3
X03128|X03128.1 Yeast gene RPO21 for RNA polymerase II largest subunit.    76  7e-15  2
Z74188|Z74188.1 S.cerevisiae chromosome IV reading frame ORF YDL140c.    72  1e-13  2
AL394664|AL394664.1 T3 end of clone AR0AA018F07 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    52  2e-13  3
X96876|X96876.1 S.cerevisiae DNA of cosmid from chromosome IV, strain FY 1679.    72  4e-13  2
U24217|U24217.1 Kluyveromyces lactis RNA polymerase II largest subunit gene, partial cds.    74  9e-11  2
M17420|M17420.1 Saccharomyces cerevisiae heptapeptide repeat region DNA.    72  7e-09  1
AL397801|AL397801.1 T3 end of clone AS0AA005C03 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    64  1e-08  2
AL400803|AL400803.1 T3 end of clone AS0AA023H06 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    64  1e-08  2
AL424996|AL424996.1 clone BA0AB002F05 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    68  1e-07  1
dna update 2003. 7.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ223185_1(DQ223185|pid:none) Glaucocystis nostochinearum strain...   103   3e-21
(P36594) RecName: Full=DNA-directed RNA polymerase II subunit rp...   101   8e-21
CR382129_619(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...   100   2e-20
FN392321_582(FN392321|pid:none) Pichia pastoris GS115 chromosome...   100   3e-20
S14183(S14183) DNA-directed RNA polymerase (EC 2.7.7.6) largest ...    99   5e-20
CR382133_628(CR382133|pid:none) Debaryomyces hansenii chromosome...    98   9e-20
AY310198_1(AY310198|pid:none) Glomeridesmus trinidadensis vouche...    97   2e-19
X52494_1(X52494|pid:none) Arabidopsis thaliana RPB1 gene for the...    97   2e-19
X52954_1(X52954|pid:none) Arabidopsis thaliana rpII gene for RNA...    97   2e-19
JDMU1(T04690;S12071;S27346;S11960)DNA-directed RNA polymerase (E...    97   2e-19
protein update 2009. 6. 6
PSORT 

 psg: 0.87  gvh: 0.39  alm: 0.49  top: 0.70  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SLJ186 is cyt
5' end seq. ID SLJ186F
5' end seq. 
>SLJ186F.Seq
AGGAAATCATTAACATTGCCAAACAAGTGAAAACACCATCCCTCACCATCTATTTGAAAC
CACATATGGCTCGTGATATGGATCGTGCAAAGATTGTAAAATCACAACTCGAATATACTA
CACTTGCAAATGTAACCTCTGCAACCGAAATCTACTATGATCCTGATCCACAAAATACCA
TCATCAGTGAAGATGCTGAATTTGTAAATAGTTATTTCGAA----------
Length of 5' end seq. 221
3' end seq. ID SLJ186Z
3' end seq. 
>SLJ186Z.Seq
----------CACCAACCTCACCATCTTATTCACCAACCTCACCATCATATTCACCATCA
TCCCCATCTTATTCACCAAGTTCGCCATCATATTCACCAAGTTCACCATCATACTCACCA
TCATCACCAACCTTTACAAACAAATATAACTATCAACCAAATAATAAAAAGAAATAGATC
AACATTAAGATACCATTTCCTATTTTAAATATTTAATTTGTATATAAATAAATTTAAACT
TAACAAATTAACAAAATATAAAAAAAAAAATAAACAA
Length of 3' end seq. 267
Connected seq. ID SLJ186P
Connected seq. 
>SLJ186P.Seq
AGGAAATCATTAACATTGCCAAACAAGTGAAAACACCATCCCTCACCATCTATTTGAAAC
CACATATGGCTCGTGATATGGATCGTGCAAAGATTGTAAAATCACAACTCGAATATACTA
CACTTGCAAATGTAACCTCTGCAACCGAAATCTACTATGATCCTGATCCACAAAATACCA
TCATCAGTGAAGATGCTGAATTTGTAAATAGTTATTTCGAA----------CACCAACCT
CACCATCTTATTCACCAACCTCACCATCATATTCACCATCATCCCCATCTTATTCACCAA
GTTCGCCATCATATTCACCAAGTTCACCATCATACTCACCATCATCACCAACCTTTACAA
ACAAATATAACTATCAACCAAATAATAAAAAGAAATAGATCAACATTAAGATACCATTTC
CTATTTTAAATATTTAATTTGTATATAAATAAATTTAAACTTAACAAATTAACAAAATAT
AAAAAAAAAAATAAACAA
Length of connected seq. 488
Full length Seq ID -
Full length Seq. -
Length of full length seq. -