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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLJ163
Library SL
(Link to library)
Clone ID SLJ163
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLJ163E
(Link to Original site)
Representative DNA sequence 
>SLJ163 (SLJ163Q) /CSM/SL/SLJ1-C/SLJ163Q.Seq.d/
GCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATG
TGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGA
CAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGT
GATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAA
ATAGCATTTTATAATAATTTTAAAAAAAA
sequence update 1999. 7.23
Translated Amino Acid sequence 
AVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAG
DKKGAAAPSKKK*innis**iafynnfkk
Translated Amino Acid sequence (All Frames) 
Frame A:
AVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAG
DKKGAAAPSKKK*innis**iafynnfkk


Frame B:
pss*rmvmplwsn*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpv
irkvllphqkrnk*iiflnk*hfiiilkk


Frame C:
rrlkew*crygrinpissnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr*
*ercccpikkeink*yflinsil**f*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                        517   e-146
SLK554 (SLK554Q) /CSM/SL/SLK5-C/SLK554Q.Seq.d/                        517   e-146
SLK466 (SLK466Q) /CSM/SL/SLK4-C/SLK466Q.Seq.d/                        517   e-146
SLK447 (SLK447Q) /CSM/SL/SLK4-B/SLK447Q.Seq.d/                        517   e-146
SLJ877 (SLJ877Q) /CSM/SL/SLJ8-D/SLJ877Q.Seq.d/                        517   e-146
SLJ355 (SLJ355Q) /CSM/SL/SLJ3-C/SLJ355Q.Seq.d/                        517   e-146
SLJ258 (SLJ258Q) /CSM/SL/SLJ2-C/SLJ258Q.Seq.d/                        517   e-146
SLJ163 (SLJ163Q) /CSM/SL/SLJ1-C/SLJ163Q.Seq.d/                        517   e-146
SLH214 (SLH214Q) /CSM/SL/SLH2-A/SLH214Q.Seq.d/                        517   e-146
SLG782 (SLG782Q) /CSM/SL/SLG7-D/SLG782Q.Seq.d/                        517   e-146
own update 2001. 8.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   408  e-110  1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   387  e-104  1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   188  2e-44  1
AZ932350|AZ932350.1 474.dhz97h09.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz97h09.s1, DNA sequence.   111  1e-23  2
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   117  7e-23  1
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   117  7e-23  1
AL399730|AL399730.1 T7 end of clone AS0AA017B02 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.   103  1e-18  1
M10992|M10992.1 Yeast (S.cerevisiae) EF-1-alpha-* (elongation factor 1-alpha) gene (TEF1), complete cds.   103  1e-18  1
M15666|M15666.1 S.cerevisiae EF1 alpha-A gene encoding polypeptide chain elongation factor 1-alpha, complete cds.   103  1e-18  1
X01638|X01638.1 Yeast TEF1 gene for elongation factor EF-1 alpha.   103  1e-18  1
dna update 2003. 7.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   105   6e-22
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   105   6e-22
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   105   6e-22
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...    87   1e-16
FJ539138_1(FJ539138|pid:none) Ignatius tetrasporus strain UTEX B...    87   2e-16
A29820_1(A29820|pid:none) A.gossypi translation elongation facto...    87   2e-16
FB784609_1(FB784609|pid:none) Sequence 3882 from Patent WO200803...    87   2e-16
AY264216_1(AY264216|pid:none) Pterodoras granulosus elongation f...    86   3e-16
AF054510_1(AF054510|pid:none) Yarrowia lipolytica translation el...    86   3e-16
AB126336_1(AB126336|pid:none) Nematostella vectensis EF1alpha mR...    86   3e-16
protein update 2009. 7. 5
PSORT 

 psg: 0.80  gvh: 0.31  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLJ163 is cyt
5' end seq. ID SLJ163F
5' end seq. 
>SLJ163F.Seq
GCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATG
TGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGA
CAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGT
GATAAGAAAGGTGCTGCTGCCCCATCAAAAAA----------
Length of 5' end seq. 212
3' end seq. ID SLJ163Z
3' end seq. 
>SLJ163Z.Seq
----------TGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCC
CCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAAT
AATATTTCTTAATAAATAGCATTTTATAATAATTTTAAAAAAAA
Length of 3' end seq. 154
Connected seq. ID SLJ163P
Connected seq. 
>SLJ163P.Seq
GCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATG
TGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGA
CAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGT
GATAAGAAAGGTGCTGCTGCCCCATCAAAAAA----------TGAGACAAACCGTCGCCG
TCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTG
CTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTATA
ATAATTTTAAAAAAAA
Length of connected seq. 366
Full length Seq ID SLJ163E
Full length Seq. 
>SLJ163E.Seq
GCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATG
TGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGA
CAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGT
GATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAA
ATAGCATTTTATAATAATTTTAAAAAAAA
Length of full length seq. 269