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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK555
Library AF
(Link to library)
Clone ID AFK555
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16358-1
Original site URL
Representative seq. ID AFK555P
(Link to Original site)
Representative DNA sequence 
>AFK555 (AFK555Q) /CSM/AF/AFK5-C/AFK555Q.Seq.d/
ACGAATATCCATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGAGTCTTCA
ATGGAAAATGGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAATAATAAAG
TTATTGCAACTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAAGCAATGT
TAGCAGCAAAAGTAAAATGGGCATTAACACCAGCTCCAAGAAGAGGTGAAATTGTTAGAT
TAATTGGTCAAGCCATGAGAGAAAAAATTGAACCATTATCAAAATTAATTTCATTAGAAA
TGGGTAAAATTTACATTGAAGCTAAAGGTGAAGTACAAGAATTCATTGACGTATGCGATT
ATGCAACTGGTTTAAGTAGATCAATCAATGGTCAAGTGATGCCATCTGAACGTCCAAATC
ACATTTTAATGGAAACCTGGAATCCATTAGGTTTAGTTGGTATCATTACTGCTTTCAATT
TCCCATGTGCCGTACTTGGTTGGAATGCTGCAATCTCAATGATTTGCGGTAACGTCCAAT
TATGGAAAGGTGCXXXXXXXXXXGAAATAAAAAANCAAGGNGGTAANGTNGNCATTGGTG
GTAATAAACTCGGATATCAGTGGTGGAAACTTTGTTNNACCNACCGNCGTTGCCNTTGAA
CANGATGCTCCNATNGTAAAGACTGAACTCTTTGNTCCNATTCTNTACATAATGAAATTC
NAAAATNTTGNCNATGCTTTTGCCTGGAATAATNAAGTCCCNCAAGGTNTCTCNTCTTCC
CTCTTCACAAACNATCNAAAGAATATCNTCCAATGGCTTGGNCCNGCCGGATCTGACTGT
GGNANAGTTAACGTNAATGNAGCNACNAACGGNGCTGNAATTGGTGGNGCCCTTGGAGGT
GAAAAANNGA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
EYPFLAELGIKAENNDGVFNGKWGGAGEIIKCLNPTNNKVIATVRGAAPEEYETCIQAML
AAKVKWALTPAPRRGEIVRLIGQAMREKIEPLSKLISLEMGKIYIEAKGEVQEFIDVCDY
ATGLSRSINGQVMPSERPNHILMETWNPLGLVGIITAFNFPCAVLGWNAAISMICGNVQL
WKG---

---EIKXQGGXVXIGGNKLGYQWWKLCXTXRRCX*txcsxxkd*tlxsxsxhneixkxxx
cfcle*xspxrxlxfplhkxskeyxpmawxxri*lwxs*rxxsxxrxxnwwxpwr*kx
Translated Amino Acid sequence (All Frames) 
Frame A:
tnihswln*alklkimmessmengevlvkssnv*iqqiikllqllevlhqknmkhvfkqc
*qqk*ngh*hqlqeevklld*lvkp*ekklnhyqn*fh*kwvkftlklkvkyknsltyai
mqlv*vdqsmvk*chlnvqitf*wkpgih*v*lvslllsishvpylvgmlqsq*favtsn
ygkv---

---EIKXQGGXVXIGGNKLGYQWWKLCXTXRRCX*txcsxxkd*tlxsxsxhneixkxxx
cfcle*xspxrxlxfplhkxskeyxpmawxxri*lwxs*rxxsxxrxxnwwxpwr*kx

Frame B:
risilg*irh*s*k**wslqwkmgrcw*nhqmfksnk**sycnc*rcctrri*nmyssnv
sskskmgintsskkr*nc*inwssherkn*tiikinfirng*nlh*s*r*strih*rmrl
cnwfk*inqwssdai*tskshfngnlesirfswyhycfqfpmcrtwleccnlndlr*rpi
merc---

---k*kxkxvxxxlvvinsdisggnfvxptxvaxexdapxvktelfxpilyimkfxnxxx
afawnnxvpqgxssslftnxxknixqwlgpagsdcgxvnvnxatngaxiggalggekx

Frame C:
EYPFLAELGIKAENNDGVFNGKWGGAGEIIKCLNPTNNKVIATVRGAAPEEYETCIQAML
AAKVKWALTPAPRRGEIVRLIGQAMREKIEPLSKLISLEMGKIYIEAKGEVQEFIDVCDY
ATGLSRSINGQVMPSERPNHILMETWNPLGLVGIITAFNFPCAVLGWNAAISMICGNVQL
WKG---

---nkkxrx*xxhww**trisvvetlxxxpxlplnxmlxx*rlnslxxfxt**nskxlxm
llpgixksxkvsxlpssqtixrissnglxxpdltvxxltxmxxxtxlxlvxplevkxx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL522 (VFL522Q) /CSM/VF/VFL5-A/VFL522Q.Seq.d/                       1096   0.0
VFK658 (VFK658Q) /CSM/VF/VFK6-C/VFK658Q.Seq.d/                       1096   0.0
VFJ372 (VFJ372Q) /CSM/VF/VFJ3-C/VFJ372Q.Seq.d/                       1096   0.0
VFD815 (VFD815Q) /CSM/VF/VFD8-A/VFD815Q.Seq.d/                       1096   0.0
VFB844 (VFB844Q) /CSM/VF/VFB8-B/VFB844Q.Seq.d/                       1096   0.0
SLE339 (SLE339Q) /CSM/SL/SLE3-B/SLE339Q.Seq.d/                       1096   0.0
SFK705 (SFK705Q) /CSM/SF/SFK7-A/SFK705Q.Seq.d/                       1096   0.0
SFH477 (SFH477Q) /CSM/SF/SFH4-D/SFH477Q.Seq.d/                       1096   0.0
SFE649 (SFE649Q) /CSM/SF/SFE6-C/SFE649Q.Seq.d/                       1096   0.0
FC-AA10 (FC-AA10Q) /CSM/FC/FC-AA/FC-AA10Q.Seq.d/                     1096   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   819   0.0    6
BU042811|BU042811.1 PP_LEa0014A23f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0014A23f, mRNA sequence.    74   7e-12  2
CN875989|CN875989.1 020813AARA008546HT (AARA) Royal Gala partially senescing leaf Malus x domestica cDNA clone AARA008546, mRNA sequence.    72   2e-08  1
D88434|D88434.1 Malus domestica mRNA for protein abundantly expressed during apple fruit development, complete cds.    72   2e-08  1
CN903667|CN903667.1 021018ABCA005552HT (ABCA) Royal Gala fruit stored at 0.5 degrees C for 24 hours Malus x domestica cDNA clone ABCA005552, mRNA sequence.    72   2e-08  1
CN883223|CN883223.1 010813AASB004877HT (AASB) Royal Gala 10 DAFB fruit Malus x domestica cDNA clone AASB004877, mRNA sequence.    72   2e-08  1
CN895049|CN895049.1 010626AAYA004321HT (AAYA) Royal Gala 126 DAFB fruit cortex Malus x domestica cDNA clone AAYA004321, mRNA sequence.    64   4e-06  1
CN902319|CN902319.1 021014ABCA004075HT (ABCA) Royal Gala fruit stored at 0.5 degrees C for 24 hours Malus x domestica cDNA clone ABCA004075, mRNA sequence.    64   4e-06  1
AY034889|AY034889.1 Tortula ruralis aldehyde dehydrogenase ALDH2 (RNP129b) mRNA, complete cds.    46   4e-06  2
BG444603|BG444603.1 GA__Ea0024O05f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ea0024O05f, mRNA sequence.    42   2e-04  2
dna update 2004. 8. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P83401) RecName: Full=Putative aldehyde dehydrogenase family 7 ...   378   e-103
BC044367_1(BC044367|pid:none) Danio rerio aldehyde dehydrogenase...   243   5e-63
BT044648_1(BT044648|pid:none) Salmo salar clone ssal-rgf-001-326...   239   9e-62
EF678715_1(EF678715|pid:none) Picea sitchensis clone WS0295_M17 ...   238   3e-61
AF323586_1(AF323586|pid:none) Oryza sativa aldehyde dehydrogenas...   234   4e-60
AY847462_1(AY847462|pid:none) Acanthopagrus schlegelii antiquiti...   233   5e-60
AK297365_1(AK297365|pid:none) Homo sapiens cDNA FLJ56008 complet...   232   1e-59
(P49419) RecName: Full=Alpha-aminoadipic semialdehyde dehydrogen...   232   1e-59
BT067707_1(BT067707|pid:none) Zea mays full-length cDNA clone ZM...   230   5e-59
AK160117_1(AK160117|pid:none) Mus musculus osteoclast-like cell ...   228   2e-58
protein update 2009. 4.24
PSORT 

 psg: 0.75  gvh: 0.31  alm: 0.29  top: 0.63  tms: 0.07  mit: 0.08  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFK555 is nuc
5' end seq. ID AFK555F
5' end seq. 
>AFK555F.Seq
ACGAATATCCATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGAGTCTTCA
ATGGAAAATGGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAATAATAAAG
TTATTGCAACTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAAGCAATGT
TAGCAGCAAAAGTAAAATGGGCATTAACACCAGCTCCAAGAAGAGGTGAAATTGTTAGAT
TAATTGGTCAAGCCATGAGAGAAAAAATTGAACCATTATCAAAATTAATTTCATTAGAAA
TGGGTAAAATTTACATTGAAGCTAAAGGTGAAGTACAAGAATTCATTGACGTATGCGATT
ATGCAACTGGTTTAAGTAGATCAATCAATGGTCAAGTGATGCCATCTGAACGTCCAAATC
ACATTTTAATGGAAACCTGGAATCCATTAGGTTTAGTTGGTATCATTACTGCTTTCAATT
TCCCATGTGCCGTACTTGGTTGGAATGCTGCAATCTCAATGATTTGCGGTAACGTCCAAT
TATGGAAAGGTGC----------
Length of 5' end seq. 553
3' end seq. ID AFK555Z
3' end seq. 
>AFK555Z.Seq
----------GAAATAAAAAANCAAGGNGGTAANGTNGNCATTGGTGGTAATAAACTCGG
ATATCAGTGGTGGAAACTTTGTTNNACCNACCGNCGTTGCCNTTGAACANGATGCTCCNA
TNGTAAAGACTGAACTCTTTGNTCCNATTCTNTACATAATGAAATTCNAAAATNTTGNCN
ATGCTTTTGCCTGGAATAATNAAGTCCCNCAAGGTNTCTCNTCTTCCCTCTTCACAAACN
ATCNAAAGAATATCNTCCAATGGCTTGGNCCNGCCGGATCTGACTGTGGNANAGTTAACG
TNAATGNAGCNACNAACGGNGCTGNAATTGGTGGNGCCCTTGGAGGTGAAAAANNGA
Length of 3' end seq. 347
Connected seq. ID AFK555P
Connected seq. 
>AFK555P.Seq
ACGAATATCCATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGAGTCTTCA
ATGGAAAATGGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAATAATAAAG
TTATTGCAACTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAAGCAATGT
TAGCAGCAAAAGTAAAATGGGCATTAACACCAGCTCCAAGAAGAGGTGAAATTGTTAGAT
TAATTGGTCAAGCCATGAGAGAAAAAATTGAACCATTATCAAAATTAATTTCATTAGAAA
TGGGTAAAATTTACATTGAAGCTAAAGGTGAAGTACAAGAATTCATTGACGTATGCGATT
ATGCAACTGGTTTAAGTAGATCAATCAATGGTCAAGTGATGCCATCTGAACGTCCAAATC
ACATTTTAATGGAAACCTGGAATCCATTAGGTTTAGTTGGTATCATTACTGCTTTCAATT
TCCCATGTGCCGTACTTGGTTGGAATGCTGCAATCTCAATGATTTGCGGTAACGTCCAAT
TATGGAAAGGTGC----------GAAATAAAAAANCAAGGNGGTAANGTNGNCATTGGTG
GTAATAAACTCGGATATCAGTGGTGGAAACTTTGTTNNACCNACCGNCGTTGCCNTTGAA
CANGATGCTCCNATNGTAAAGACTGAACTCTTTGNTCCNATTCTNTACATAATGAAATTC
NAAAATNTTGNCNATGCTTTTGCCTGGAATAATNAAGTCCCNCAAGGTNTCTCNTCTTCC
CTCTTCACAAACNATCNAAAGAATATCNTCCAATGGCTTGGNCCNGCCGGATCTGACTGT
GGNANAGTTAACGTNAATGNAGCNACNAACGGNGCTGNAATTGGTGGNGCCCTTGGAGGT
GAAAAANNGA
Length of connected seq. 900
Full length Seq ID -
Full length Seq. -
Length of full length seq. -