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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLI827
Library SL
(Link to library)
Clone ID SLI827
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12059-1|Contig-U15509-1
Original site URL
Representative seq. ID SLI827P
(Link to Original site)
Representative DNA sequence 
>SLI827 (SLI827Q) /CSM/SL/SLI8-B/SLI827Q.Seq.d/
AGCCAATGATCCAATCGATGAGAATGATAATGGTTTAGTATTAACCCAAGCAGGTAAAAG
ATATGCAATCACCTATCAATTAAGTCACGCAATTGATAACACCAATAAAGAATTAATAGT
TCAATATGAGTTTCAATTCCAAGAGGGTGTAACATGTTCAGGTGGTTACATTAAATTGTA
CACAGATCGTGAAGATTTCAATGTGGAAACTGTCGACTCAAACACCCCATACAGCATCAT
GTTTGGCXXXXXXXXXXAGGTGATATCTTTGAAACTATTAAATTCTATTTAACTCTCTTC
CAATCTGAAGCAAATCAAAATCCATTACTTTATTTAGTTGGTTTCTCTTCTTTACTTTTA
CCAATTGTATTTTGTTTCACTCGTTCAAAGAAACCATCATCAAAAAAAGTTAAATCTGAT
GATGATAATAATTCAACCTCAACTACCACCACCAATACTTCAGTCACTAAAGAATCTGTT
TCAATTCAAGATAAACCAACTATTGAATCTGAAGAATCTGATGAATCTGATGAAGATAAT
GAAACTACTAAAAAATCAACTGTTACAAAAAGAACTAATAAAGTTAAATAAAAAGTTAAA
ATAATTTTTTAAATTTTAAAAAAAAAAAAAAAAAA
sequence update 1999. 2.23
Translated Amino Acid sequence 
ANDPIDENDNGLVLTQAGKRYAITYQLSHAIDNTNKELIVQYEFQFQEGVTCSGGYIKLY
TDREDFNVETVDSNTPYSIMFG---

---GDIFETIKFYLTLFQSEANQNPLLYLVGFSSLLLPIVFCFTRSKKPSSKKVKSDDDN
NSTSTTTTNTSVTKESVSIQDKPTIESEESDESDEDNETTKKSTVTKRTNKVK*kvkiif
*ilkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
sq*snr*e**wfsinpsr*kicnhlsiksrn**hq*rinssi*vsiprgcnmfrwlh*iv
hrs*rfqcgncrlkhpiqhhvw---

---r*yl*ny*ilfnslpi*sksksitlfswflfftftncilfhsfketiikks*i****
*fnlnyhhqyfsh*ricfnsr*tny*i*ri**i**r**ny*kincykkn**s*iks*nnf
lnfkkkkkk

Frame B:
ANDPIDENDNGLVLTQAGKRYAITYQLSHAIDNTNKELIVQYEFQFQEGVTCSGGYIKLY
TDREDFNVETVDSNTPYSIMFG---

---GDIFETIKFYLTLFQSEANQNPLLYLVGFSSLLLPIVFCFTRSKKPSSKKVKSDDDN
NSTSTTTTNTSVTKESVSIQDKPTIESEESDESDEDNETTKKSTVTKRTNKVK*kvkiif
*ilkkkkk

Frame C:
pmiqsmrmimv*y*pkqvkdmqspin*vtqlitpikn**fnmsfnskrv*hvqvvtlnct
qivkismwklstqtphtascl---

---vislkllnsi*lssnlkqikihyfi*lvsllyfyqlyfvslvqrnhhqkklnlmmii
iqpqlpppilqslknlfqfkinqllnlknlmnlmkimkllknqllqkeliklnkklk*ff
kf*kkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLI827 (SLI827Q) /CSM/SL/SLI8-B/SLI827Q.Seq.d/                        971   0.0
VFM752 (VFM752Q) /CSM/VF/VFM7-C/VFM752Q.Seq.d/                        521   e-147
SLI776 (SLI776Q) /CSM/SL/SLI7-D/SLI776Q.Seq.d/                        521   e-147
SFJ457 (SFJ457Q) /CSM/SF/SFJ4-C/SFJ457Q.Seq.d/                        521   e-147
FC-BL22 (FC-BL22Q) /CSM/FC/FC-BL/FC-BL22Q.Seq.d/                      521   e-147
AFE582 (AFE582Q) /CSM/AF/AFE5-D/AFE582Q.Seq.d/                        521   e-147
CHJ720 (CHJ720Q) /CSM/CH/CHJ7-A/CHJ720Q.Seq.d/                        511   e-144
AFA253 (AFA253Q) /CSM/AF/AFA2-C/AFA253Q.Seq.d/                        474   e-133
VHE413 (VHE413Q) /CSM/VH/VHE4-A/VHE413Q.Seq.d/                         44   0.003
CHQ275 (CHQ275Q) /CSM/CH/CHQ2-D/CHQ275Q.Seq.d/                         44   0.003
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF073837|AF073837.1 Dictyostelium discoideum calnexin precursor, gene, complete cds.   482  0.0  3
AF019376|AF019376.1 Brassica napus calreticulin mRNA, complete cds.    50  0.005  2
AC116979|AC116979.2 Dictyostelium discoideum chromosome 2 map 6357117-6445670 strain AX4, complete sequence.    32  0.030  6
CD470851|CD470851.1 LeukoS5_2_C12.g1_A027 Stimulated peripheral blood leukocytes S5 Equus caballus cDNA clone LeukoS5_2_C12_A027 5', mRNA sequence.    50  0.033  1
CD428924|CD428924.1 ETH1_1_F06.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_1_F06_A002 5', mRNA sequence.    50  0.033  1
CD836033|CD836033.1 BN45.047H13F020102 BN45 Brassica napus cDNA clone BN45047H13, mRNA sequence.    50  0.033  1
CD837819|CD837819.1 BN45.053K17F020107 BN45 Brassica napus cDNA clone BN45053K17, mRNA sequence.    50  0.033  1
CD231647|CD231647.1 SS1_22_E10.g1_A012 Salt-stressed seedlings Sorghum bicolor cDNA clone SS1_22_E10_A012 5', mRNA sequence.    50  0.033  1
CD813739|CD813739.1 BN15.020N09F020211 BN15 Brassica napus cDNA clone BN15020N09, mRNA sequence.    50  0.033  1
CD825882|CD825882.1 BN25.062B18F011130 BN25 Brassica napus cDNA clone BN25062B18, mRNA sequence.    50  0.033  1
dna update 2003. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC124496_1(BC124496|pid:none) Danio rerio calmegin, mRNA (cDNA c...    74   3e-12
AJ719429_1(AJ719429|pid:none) Gallus gallus mRNA for hypothetica...    69   1e-10
AB047037_1(AB047037|pid:none) Halocynthia roretzi HrCalnexin mRN...    68   2e-10
(Q3SYT6) RecName: Full=Calmegin; Flags: Precursor;  &BC103401_1(...    68   2e-10
DQ381152_1(DQ381152|pid:none) Bos taurus BTA17 39037 genomic seq...    68   2e-10
EU886196_8(EU886196|pid:none) Beauveria bassiana strain ATCC 715...    68   2e-10
FN314644_1(FN314644|pid:none) Schistosoma japonicum isolate Anhu...    68   2e-10
A46637(A46637) calnexin homolog SmIrV1 - fluke (Schistosoma mans...    68   2e-10
(P35565) RecName: Full=Calnexin; Flags: Precursor;  &C54354(C543...    68   2e-10
AY100687_1(AY100687|pid:none) Cricetulus griseus calnexin mRNA, ...    68   2e-10
protein update 2009. 6. 5
PSORT 

 psg: 0.75  gvh: 0.32  alm: 0.25  top: 0.80  tms: 0.07  mit: 0.22  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.11  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for SLI827 is cyt
5' end seq. ID SLI827F
5' end seq. 
>SLI827F.Seq
AGCCAATGATCCAATCGATGAGAATGATAATGGTTTAGTATTAACCCAAGCAGGTAAAAG
ATATGCAATCACCTATCAATTAAGTCACGCAATTGATAACACCAATAAAGAATTAATAGT
TCAATATGAGTTTCAATTCCAAGAGGGTGTAACATGTTCAGGTGGTTACATTAAATTGTA
CACAGATCGTGAAGATTTCAATGTGGAAACTGTCGACTCAAACACCCCATACAGCATCAT
GTTTGGC----------
Length of 5' end seq. 247
3' end seq. ID SLI827Z
3' end seq. 
>SLI827Z.Seq
----------AGGTGATATCTTTGAAACTATTAAATTCTATTTAACTCTCTTCCAATCTG
AAGCAAATCAAAATCCATTACTTTATTTAGTTGGTTTCTCTTCTTTACTTTTACCAATTG
TATTTTGTTTCACTCGTTCAAAGAAACCATCATCAAAAAAAGTTAAATCTGATGATGATA
ATAATTCAACCTCAACTACCACCACCAATACTTCAGTCACTAAAGAATCTGTTTCAATTC
AAGATAAACCAACTATTGAATCTGAAGAATCTGATGAATCTGATGAAGATAATGAAACTA
CTAAAAAATCAACTGTTACAAAAAGAACTAATAAAGTTAAATAAAAAGTTAAAATAATTT
TTTAAATTTTAAAAAAAAAAAAAAAAAA
Length of 3' end seq. 378
Connected seq. ID SLI827P
Connected seq. 
>SLI827P.Seq
AGCCAATGATCCAATCGATGAGAATGATAATGGTTTAGTATTAACCCAAGCAGGTAAAAG
ATATGCAATCACCTATCAATTAAGTCACGCAATTGATAACACCAATAAAGAATTAATAGT
TCAATATGAGTTTCAATTCCAAGAGGGTGTAACATGTTCAGGTGGTTACATTAAATTGTA
CACAGATCGTGAAGATTTCAATGTGGAAACTGTCGACTCAAACACCCCATACAGCATCAT
GTTTGGC----------AGGTGATATCTTTGAAACTATTAAATTCTATTTAACTCTCTTC
CAATCTGAAGCAAATCAAAATCCATTACTTTATTTAGTTGGTTTCTCTTCTTTACTTTTA
CCAATTGTATTTTGTTTCACTCGTTCAAAGAAACCATCATCAAAAAAAGTTAAATCTGAT
GATGATAATAATTCAACCTCAACTACCACCACCAATACTTCAGTCACTAAAGAATCTGTT
TCAATTCAAGATAAACCAACTATTGAATCTGAAGAATCTGATGAATCTGATGAAGATAAT
GAAACTACTAAAAAATCAACTGTTACAAAAAGAACTAATAAAGTTAAATAAAAAGTTAAA
ATAATTTTTTAAATTTTAAAAAAAAAAAAAAAAAA
Length of connected seq. 625
Full length Seq ID -
Full length Seq. -
Length of full length seq. -