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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLI621
Library SL
(Link to library)
Clone ID SLI621
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14948-1
Original site URL
Representative seq. ID SLI621P
(Link to Original site)
Representative DNA sequence 
>SLI621 (SLI621Q) /CSM/SL/SLI6-A/SLI621Q.Seq.d/
CTTTATCTTACTTTTTACCATACAATTCAAACTTTTTTGTATAGTTTTTTATAATTTTAA
AGAAAAAATAAAAAGATGTCAGCTGTTGATGATCAACAAAATGCAGATAAGCAAGTTGAA
CAATGGAAAATCAAAAAATTAATTAAAAATTTGGAAGCTGCCAGAGGTAATGGTACATCX
XXXXXXXXXAAGAATCTGGTCTTGACTATGAAATCGTCGAAGAAATGCCAATCGTTGAGT
GGTTCGCCAATAACTACAAAAACTTTGGTGCCTCTCTCGAATTCGTCACCAACAAATCTC
AAGAAGGTTCACAATTCTGTAAAGGTTTTGGTGGTTTGGGTGGTTTACTCCGTTATCAAG
TAGATTTCGCTCAACTCAATGATTTCGATAATCCAGATGAGAATGAATACGATGATAGTG
ATTCAGACTTTTGACCAATTGAAAAAACCAGCAACAGAAATGGTACTGGTTTGGTCTCCT
CCACTTTTAAGGTTGCCCCTTCCTTCTCTACCATTCAAAAACAACAACAACAACAACAAA
AATCACTTTCACCAAAAACAACATTAACAACAATACCAACACTCGTTCTCAACAAATAAA
TTTAGTAACAAATTAAAGAAAATAAAAAAAT
sequence update 1999. 2.23
Translated Amino Acid sequence 
lyltfyhtiqtflysfl*f*RKNKKMSAVDDQQNADKQVEQWKIKKLIKNLEAARGNGT-
--

---ESGLDYEIVEEMPIVEWFANNYKNFGASLEFVTNKSQEGSQFCKGFGGLGGLLRYQV
DFAQLNDFDNPDENEYDDSDSDF*piektsnrngtglvsstfkvapsfstiqkqqqqqqk
slspkttlttiptlvlnk*i**qikenkk
Translated Amino Acid sequence (All Frames) 
Frame A:
lyltfyhtiqtflysfl*f*RKNKKMSAVDDQQNADKQVEQWKIKKLIKNLEAARGNGT-
--

---knlvltmksskkcqslsgspittktlvplsnssptnlkkvhnsvkvlvvwvvysvik
*islnsmisiiqmrmntmiviqtfdqlkkpatemvlvwsppllrlplpslpfknnnnnnk
nhfhqkqh*qqyqhsfstnkfsnklkkikk

Frame B:
fillftiqfklfcivfynfkekikrcqllminkmqisklnngkskn*lkiwklpevmvh-
--

---riws*l*nrrrnanr*vvrq*lqklwclsrirhqqisrrftil*rfwwfgwftplss
rfrstq*fr*sr*e*ir***frlltn*knqqqkwywfgllhf*gcpfllyhskttttttk
itftknninnntntrsqqinlvtn*rk*kn

Frame C:
lsyflpynsnffv*ffiilkkk*kdvsc**stkcr*as*tmenqkin*kfgscqr*wyi-
--

---ESGLDYEIVEEMPIVEWFANNYKNFGASLEFVTNKSQEGSQFCKGFGGLGGLLRYQV
DFAQLNDFDNPDENEYDDSDSDF*piektsnrngtglvsstfkvapsfstiqkqqqqqqk
slspkttlttiptlvlnk*i**qikenkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLI621 (SLI621Q) /CSM/SL/SLI6-A/SLI621Q.Seq.d/                        664   0.0
VFM203 (VFM203Q) /CSM/VF/VFM2-A/VFM203Q.Seq.d/                        644   0.0
VFK387 (VFK387Q) /CSM/VF/VFK3-D/VFK387Q.Seq.d/                        644   0.0
VFK364 (VFK364Q) /CSM/VF/VFK3-C/VFK364Q.Seq.d/                        644   0.0
VFI434 (VFI434Q) /CSM/VF/VFI4-B/VFI434Q.Seq.d/                        644   0.0
VFD523 (VFD523Q) /CSM/VF/VFD5-A/VFD523Q.Seq.d/                        644   0.0
SFL448 (SFL448Q) /CSM/SF/SFL4-B/SFL448Q.Seq.d/                        644   0.0
CHG846 (CHG846Q) /CSM/CH/CHG8-B/CHG846Q.Seq.d/                        644   0.0
AFH772 (AFH772Q) /CSM/AF/AFH7-C/AFH772Q.Seq.d/                        644   0.0
AFE158 (AFE158Q) /CSM/AF/AFE1-C/AFE158Q.Seq.d/                        644   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF298834|AF298834.1 Dictyostelium discoideum eukaryotic release factor 1 (erf1) mRNA, complete cds.   644  0.0  4
BQ453108|BQ453108.1 sao96a12.y1 Gm-c1081 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1081-3480 5' similar to SW:ERF1_ARATH P35614 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    74  2e-09  1
AC135505|AC135505.11 Medicago truncatula clone mth2-24j8, WORKING DRAFT SEQUENCE, 2 ordered pieces.    68  4e-08  3
AC136505|AC136505.11 Medicago truncatula clone mth2-24f15, WORKING DRAFT SEQUENCE, 9 unordered pieces.    58  5e-08  4
AW201445|AW201445.1 sf03c02.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-1179 5' similar to SW:ERF1_ARATH P35614 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    68  1e-07  1
BG790012|BG790012.1 sae61h11.y1 Gm-c1064 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1064-3045 5' similar to SW:ERF1_ARATH P35614 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    60  3e-05  1
AW757177|AW757177.1 sl30b03.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-2790 5' similar to SW:ERF1_ARATH P35614 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    60  3e-05  1
AW348273|AW348273.1 GM210001B23A11R Gm-r1021 Glycine max cDNA clone Gm-r1021-46 3', mRNA sequence.    60  3e-05  1
AW831104|AW831104.1 sm08g04.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-8335 5' similar to SW:ERF1_ARATH P35614 EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1 ;, mRNA sequence.    60  3e-05  1
AW350846|AW350846.1 GM210010B10B10R Gm-r1021 Glycine max cDNA clone Gm-r1021-3547 3', mRNA sequence.    60  3e-05  1
dna update 2003. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF298834_1(AF298834|pid:none) Dictyostelium discoideum eukaryoti...   149   6e-35
AC136505_15(AC136505|pid:none) Medicago truncatula clone mth2-24...    85   2e-15
(O16520) RecName: Full=Eukaryotic peptide chain release factor s...    85   2e-15
BT074577_1(BT074577|pid:none) Osmerus mordax clone omor-eva-515-...    84   4e-15
CR382135_224(CR382135|pid:none) Debaryomyces hansenii strain CBS...    83   7e-15
(P35614) RecName: Full=Eukaryotic peptide chain release factor s...    82   9e-15
U40218_1(U40218|pid:none) Arabidopsis thaliana eukaryotic releas...    82   9e-15
DQ241835_1(DQ241835|pid:none) Solanum tuberosum clone 180C08 euk...    82   9e-15
D87691_1(D87691|pid:none) Mus musculus mRNA for eRF1, partial cds.     82   2e-14
(P62498) RecName: Full=Eukaryotic peptide chain release factor s...    82   2e-14
protein update 2009. 6. 5
PSORT 

 psg: 0.43  gvh: 0.30  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SLI621 is nuc
5' end seq. ID SLI621F
5' end seq. 
>SLI621F.Seq
CTTTATCTTACTTTTTACCATACAATTCAAACTTTTTTGTATAGTTTTTTATAATTTTAA
AGAAAAAATAAAAAGATGTCAGCTGTTGATGATCAACAAAATGCAGATAAGCAAGTTGAA
CAATGGAAAATCAAAAAATTAATTAAAAATTTGGAAGCTGCCAGAGGTAATGGTACATC-
---------
Length of 5' end seq. 179
3' end seq. ID SLI621Z
3' end seq. 
>SLI621Z.Seq
----------AAGAATCTGGTCTTGACTATGAAATCGTCGAAGAAATGCCAATCGTTGAG
TGGTTCGCCAATAACTACAAAAACTTTGGTGCCTCTCTCGAATTCGTCACCAACAAATCT
CAAGAAGGTTCACAATTCTGTAAAGGTTTTGGTGGTTTGGGTGGTTTACTCCGTTATCAA
GTAGATTTCGCTCAACTCAATGATTTCGATAATCCAGATGAGAATGAATACGATGATAGT
GATTCAGACTTTTGACCAATTGAAAAAACCAGCAACAGAAATGGTACTGGTTTGGTCTCC
TCCACTTTTAAGGTTGCCCCTTCCTTCTCTACCATTCAAAAACAACAACAACAACAACAA
AAATCACTTTCACCAAAAACAACATTAACAACAATACCAACACTCGTTCTCAACAAATAA
ATTTAGTAACAAATTAAAGAAAATAAAAAAAT
Length of 3' end seq. 442
Connected seq. ID SLI621P
Connected seq. 
>SLI621P.Seq
CTTTATCTTACTTTTTACCATACAATTCAAACTTTTTTGTATAGTTTTTTATAATTTTAA
AGAAAAAATAAAAAGATGTCAGCTGTTGATGATCAACAAAATGCAGATAAGCAAGTTGAA
CAATGGAAAATCAAAAAATTAATTAAAAATTTGGAAGCTGCCAGAGGTAATGGTACATC-
---------AAGAATCTGGTCTTGACTATGAAATCGTCGAAGAAATGCCAATCGTTGAGT
GGTTCGCCAATAACTACAAAAACTTTGGTGCCTCTCTCGAATTCGTCACCAACAAATCTC
AAGAAGGTTCACAATTCTGTAAAGGTTTTGGTGGTTTGGGTGGTTTACTCCGTTATCAAG
TAGATTTCGCTCAACTCAATGATTTCGATAATCCAGATGAGAATGAATACGATGATAGTG
ATTCAGACTTTTGACCAATTGAAAAAACCAGCAACAGAAATGGTACTGGTTTGGTCTCCT
CCACTTTTAAGGTTGCCCCTTCCTTCTCTACCATTCAAAAACAACAACAACAACAACAAA
AATCACTTTCACCAAAAACAACATTAACAACAATACCAACACTCGTTCTCAACAAATAAA
TTTAGTAACAAATTAAAGAAAATAAAAAAAT
Length of connected seq. 621
Full length Seq ID -
Full length Seq. -
Length of full length seq. -