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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK539
Library AF
(Link to library)
Clone ID AFK539
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFK539Z
(Link to Original site)
Representative DNA sequence 
>AFK539 (AFK539Q) /CSM/AF/AFK5-B/AFK539Q.Seq.d/
XXXXXXXXXXATANCACTCAAGGTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAA
GGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATG
GGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGT
TACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGAT
GTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGAT
GCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAG
GATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATC
TACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGAT
GCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTC
TTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATG
AACTTTGTTGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCATCATCTCAAA
CTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---XTQGLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARY
NFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKD
VLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLF
KADDKENPISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKLMIMXI*krffc*t
Translated Amino Acid sequence (All Frames) 
Frame A:
---xhsrsrrckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*ssl
qfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkg
cfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhal
qs**qrksnll*rskk*syelc*ihsr*rchefklpssqtddnvnlkkillln


Frame B:
---XTQGLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARY
NFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKD
VLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLF
KADDKENPISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKLMIMXI*krffc*t


Frame C:
---xlkvskm*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvt
ismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrm
fwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscss
klmtkkiqslmkvkemii*tllnsfkitlp*iqitiisn***cxskkdssak
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK539 (AFK539Q) /CSM/AF/AFK5-B/AFK539Q.Seq.d/                       1360   0.0
AFM548 (AFM548Q) /CSM/AF/AFM5-B/AFM548Q.Seq.d/                       1352   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1352   0.0
AFM212 (AFM212Q) /CSM/AF/AFM2-A/AFM212Q.Seq.d/                       1350   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1346   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1346   0.0
AFJ487 (AFJ487Q) /CSM/AF/AFJ4-D/AFJ487Q.Seq.d/                       1346   0.0
AFM717 (AFM717Q) /CSM/AF/AFM7-A/AFM717Q.Seq.d/                       1342   0.0
AFJ720 (AFJ720Q) /CSM/AF/AFJ7-A/AFJ720Q.Seq.d/                       1340   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1338   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
dna update 2004. 8. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   173   4e-42
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   3e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   169   7e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   1e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   162   9e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   3e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   3e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   3e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   3e-35
protein update 2009. 4.24
PSORT 

 psg: 0.65  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for AFK539 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFK539Z
3' end seq. 
>AFK539Z.Seq
----------ATANCACTCAAGGTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAA
GGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATG
GGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGT
TACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGAT
GTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGAT
GCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAG
GATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATC
TACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGAT
GCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTC
TTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATG
AACTTTGTTGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCATCATCTCAAA
CTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACA
Length of 3' end seq. 692
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -