>AFK532 (AFK532Q) /CSM/AF/AFK5-B/AFK532Q.Seq.d/
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCXXXXXXXXXXANAATTCATTA
AANAANATCCNATCNTTTCAATTGAANATCCNTTTGATCAANATGATTGGGAATCATACC
CTNAATTAACAGCNTCAGTTGANATTCNAANTGTTGGNGATGATTTACTTGNTACCNACC
CANAAANAATTAANACTGGTATTGAAAANAAAGCATGTAATGCTNTTTTATTNAAAGTTA
ACCNAATTGGNTCAGTCNCTGAANCNATTNGNGCCGCTCTTGACNCAAANAANGCNTCAT
GGGGNGTCANGGNTANCCNTCGNTCAGGTGAAACTGAANANACCNTCNNTGCTGANTTAG
NTGTTGGNTTAGGGACCGGNCNAATTAAAACTGGNGCNCCNTGTAGATCAGAAANATT

ptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHA
GNKLAMQEFMIL---

---XFIKXXPIXSIEXPFDQXDWESYPXLTASVXIXXVGDDLLXTXPXXIXTGIEXKACN
AXLXKVNXIGSVXEXIXAALDXXXASWGVXXXXRSGETEXTXXAXLXVGLGTGXIKTGAP
CRSEX

Frame A:
thlllyfflslsiyisffkmsviksikareildsrgnptvevdlytekdgvvssfraavp
sgastgiyeavelrdgdksrylgkgvlkaiknileviqpavigksvsdqaaidklmidld
gtpnkgklganailavslavcragaadrnlplykyiseiagtkmrlpvpafnvingwftc
r**isyariydft---

---xih*xxsxxfn*xsx*sx*lgiipxinsxs*xsxcwx*ftxyxpxxn*xwy*kxsm*
cxfixs*pnwxsx*xxxxrs*xkxxxmgxxgxpsxr*n*xxxxc*xxcwxrdrxn*nwxx
x*irxi

Frame B:
ptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHA
GNKLAMQEFMIL---

---XFIKXXPIXSIEXPFDQXDWESYPXLTASVXIXXVGDDLLXTXPXXIXTGIEXKACN
AXLXKVNXIGSVXEXIXAALDXXXASWGVXXXXRSGETEXTXXAXLXVGLGTGXIKTGAP
CRSEX

Frame C:
plitlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssi
rcinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrw
ypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwvvhmq
vin*lcknl*fy---

---nslxxixsfqlxixlixmignhtln*qxqlxfxxlxmiylxptxkxlxlvlkxkhvm
lfyxkltxlxqslxxxxplltqxxxhgxsxxxxxqvklxxpsxlx*xlx*gpxxlklxxx
vdqkx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK532 (AFK532Q) /CSM/AF/AFK5-B/AFK532Q.Seq.d/                       1473   0.0
AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/                       1096   0.0
AFN159 (AFN159Q) /CSM/AF/AFN1-C/AFN159Q.Seq.d/                       1096   0.0
AFL547 (AFL547Q) /CSM/AF/AFL5-B/AFL547Q.Seq.d/                       1096   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1096   0.0
VFL491 (VFL491Q) /CSM/VF/VFL4-D/VFL491Q.Seq.d/                       1088   0.0
SFK804 (SFK804Q) /CSM/SF/SFK8-A/SFK804Q.Seq.d/                       1088   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1080   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1080   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1080   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI504877|BI504877.1 BB170011A20G10.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20G10 5', mRNA sequence.    82   2e-25  3
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   3e-25  6
AX932030|AX932030.1 Sequence 13 from Patent WO03087352.            50   3e-25  6
AX932021|AX932021.1 Sequence 4 from Patent WO03087352.             50   3e-25  6
BD237140|BD237140.1 Staphylococcus aureus gene and polypeptide.    50   6e-25  6
AR282358|AR282358.1 Sequence 21 from patent US 6521441.            50   6e-25  6
AF065394|AF065394.1 Staphylococcus aureus enolase (eno) gene, complete cds.    50   6e-25  6
AR354089|AR354089.1 Sequence 207 from patent US 6593114.           50   7e-25  6
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62   1e-24  5
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62   1e-24  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   300   1e-85
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   204   5e-57
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   204   7e-57
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   206   9e-57
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   203   1e-56
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   201   4e-56
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   201   4e-56
CR859195_1(CR859195|pid:none) Pongo abelii mRNA; cDNA DKFZp459B1...   201   4e-56
BC017249_1(BC017249|pid:none) Homo sapiens enolase 3 (beta, musc...   201   7e-56
JC1039(JC1039)phosphopyruvate hydratase (EC 4.2.1.11) - rat           201   7e-56

 psg: 0.75  gvh: 0.51  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK532 is cyt

>AFK532F.Seq
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACC----------

>AFK532Z.Seq
----------ANAATTCATTAAANAANATCCNATCNTTTCAATTGAANATCCNTTTGATC
AANATGATTGGGAATCATACCCTNAATTAACAGCNTCAGTTGANATTCNAANTGTTGGNG
ATGATTTACTTGNTACCNACCCANAAANAATTAANACTGGTATTGAAAANAAAGCATGTA
ATGCTNTTTTATTNAAAGTTAACCNAATTGGNTCAGTCNCTGAANCNATTNGNGCCGCTC
TTGACNCAAANAANGCNTCATGGGGNGTCANGGNTANCCNTCGNTCAGGTGAAACTGAAN
ANACCNTCNNTGCTGANTTAGNTGTTGGNTTAGGGACCGGNCNAATTAAAACTGGNGCNC
CNTGTAGATCAGAAANATT

>AFK532P.Seq
ACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATG
AGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTT
GAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCA
TCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGA
TACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCC
GTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGAT
GGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTT
TGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCA
GGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACC----------ANAATTCATTA
AANAANATCCNATCNTTTCAATTGAANATCCNTTTGATCAANATGATTGGGAATCATACC
CTNAATTAACAGCNTCAGTTGANATTCNAANTGTTGGNGATGATTTACTTGNTACCNACC
CANAAANAATTAANACTGGTATTGAAAANAAAGCATGTAATGCTNTTTTATTNAAAGTTA
ACCNAATTGGNTCAGTCNCTGAANCNATTNGNGCCGCTCTTGACNCAAANAANGCNTCAT
GGGGNGTCANGGNTANCCNTCGNTCAGGTGAAACTGAANANACCNTCNNTGCTGANTTAG
NTGTTGGNTTAGGGACCGGNCNAATTAAAACTGGNGCNCCNTGTAGATCAGAAANATT