>SLI250 (SLI250Q) /CSM/SL/SLI2-C/SLI250Q.Seq.d/
XXXXXXXXXXTCAACTGAATGGGAACAACAAGTTCCATCAATGGATTTAAGTTGGAAAGA
AACTATTCGTCCACTCTTTAAATATTTCACTCTTCGTACTCCAGGTTCTTTCTTTGAAGA
AAAAGAAATGTTATTCACATGGCATTATAGAAATGCTGATCCAATATTTGGTTCAATTCA
AGCACGTGAATTACATTTACATTTAGATAATTTACCTTTAGATGTTATAGTTGGTGATAA
AACATTGGGTGTAAGATCATATAATATAAATCCATTATCCTCAATGAAAAAAGTTATAAC
AGACACAATTCCAAAAGGATTAGATTTAATTTTATTAATTGGTGATACTCATATTCATCC
AAGTGAATTGCCAACATTTGATGGTAAAATCTTTAATATTTCAGTTGGTAAAAAATCTGT
TAAAGATAGTTACCATTTAAGTGATCCAGCTGAAGTTAATTATCTTTTAAATAGATTAGA
AAAAATTTCAAAAGATGAATCATGTGCTCCTGTACCTGATAATTAATTTGTTTATCAAAA
TAAAAATAAAAATTATAAATAGAATAAATAAATAAATTAATAATAAAA

---TEWEQQVPSMDLSWKETIRPLFKYFTLRTPGSFFEEKEMLFTWHYRNADPIFGSIQA
RELHLHLDNLPLDVIVGDKTLGVRSYNINPLSSMKKVITDTIPKGLDLILLIGDTHIHPS
ELPTFDGKIFNISVGKKSVKDSYHLSDPAEVNYLLNRLEKISKDESCAPVPDN*fvyqnk
nknyk*nk*in***

Frame A:
---qlngnnkfhqwi*vgkklfvhslnislfvlqvlslkkkkcyshgiiemliqylvqfk
hvnyiyi*iiyl*ml*lvikhwv*dhii*ihypq*kkl*qtqfqkd*i*fy*lvilifiq
vncqhlmvkslifqlvknllkivti*viqlkliif*id*kkfqkmnhvllyliinlfiki
kikiinrink*innk


Frame B:
---n*mgttssingfklernysstl*ifhssysrffl*rkrnvihmal*kc*sniwfnss
t*itftfr*ftfrcysw**nigckii*yksiilneksynrhnskrirfnfinw*ysyssk
*iani*w*nl*yfsw*kic*r*lpfk*ss*s*lsfk*irknfkr*imcsct**liclsk*
k*kl*ie*inkliik


Frame C:
---TEWEQQVPSMDLSWKETIRPLFKYFTLRTPGSFFEEKEMLFTWHYRNADPIFGSIQA
RELHLHLDNLPLDVIVGDKTLGVRSYNINPLSSMKKVITDTIPKGLDLILLIGDTHIHPS
ELPTFDGKIFNISVGKKSVKDSYHLSDPAEVNYLLNRLEKISKDESCAPVPDN*fvyqnk
nknyk*nk*in***

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ339 (VHQ339Q) /CSM/VH/VHQ3-B/VHQ339Q.Seq.d/                       1043   0.0
VHI354 (VHI354Q) /CSM/VH/VHI3-C/VHI354Q.Seq.d/                       1043   0.0
VHG138 (VHG138Q) /CSM/VH/VHG1-B/VHG138Q.Seq.d/                       1043   0.0
SLI250 (SLI250Q) /CSM/SL/SLI2-C/SLI250Q.Seq.d/                       1043   0.0
SHK718 (SHK718Q) /CSM/SH/SHK7-A/SHK718Q.Seq.d/                       1043   0.0
CHR506 (CHR506Q) /CSM/CH/CHR5-A/CHR506Q.Seq.d/                       1043   0.0
CHM693 (CHM693Q) /CSM/CH/CHM6-D/CHM693Q.Seq.d/                       1043   0.0
CHL450 (CHL450Q) /CSM/CH/CHL4-C/CHL450Q.Seq.d/                       1043   0.0
CHK472 (CHK472Q) /CSM/CH/CHK4-C/CHK472Q.Seq.d/                       1043   0.0
CHI681 (CHI681Q) /CSM/CH/CHI6-D/CHI681Q.Seq.d/                       1043   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU053258) Dictyostelium discoideum slug cDNA, clone SLI250.     1043   0.0    1
(BJ446660) Dictyostelium discoideum cDNA clone:ddv63n09, 3' ...  1043   0.0    1
(BJ441726) Dictyostelium discoideum cDNA clone:ddv47l13, 3' ...  1043   0.0    1
(BJ440340) Dictyostelium discoideum cDNA clone:ddv43k09, 3' ...  1043   0.0    1
(BJ408201) Dictyostelium discoideum cDNA clone:dds45d05, 3' ...  1043   0.0    1
(BJ386094) Dictyostelium discoideum cDNA clone:ddc57k01, 3' ...  1043   0.0    1
(BJ383985) Dictyostelium discoideum cDNA clone:ddc47i24, 3' ...  1043   0.0    1
(BJ382371) Dictyostelium discoideum cDNA clone:ddc44d14, 3' ...  1043   0.0    1
(BJ381809) Dictyostelium discoideum cDNA clone:ddc42p18, 3' ...  1043   0.0    1
(BJ380917) Dictyostelium discoideum cDNA clone:ddc39a22, 3' ...  1043   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K57) RecName: Full=Alpha,alpha-trehalose-phosphate synthase ...   315   6e-85
CR382130_585(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...    99   8e-20
AP005577_11(AP005577|pid:none) Oryza sativa Japonica Group genom...    94   3e-18
EU626442_4(EU626442|pid:none) Gossypium raimondii clone BAC 031J...    91   2e-17
EU626443_2(EU626443|pid:none) Gossypioides kirkii clone BAC 096G...    90   4e-17
AM477810_1(AM477810|pid:none) Vitis vinifera contig VV78X188344....    90   5e-17
AK228947_1(AK228947|pid:none) Arabidopsis thaliana mRNA for puta...    89   7e-17
(Q9LMI0) RecName: Full=Probable alpha,alpha-trehalose-phosphate ...    89   7e-17
AC183498_50(AC183498|pid:none) Brassica oleracea, *** SEQUENCING...    88   1e-16
AY714859_12(AY714859|pid:none) Uncultured archaeon GZfos34A6 clo...    87   4e-16

 psg: 0.75  gvh: 0.35  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for SLI250 is cyt

>SLI250Z.Seq
----------TCAACTGAATGGGAACAACAAGTTCCATCAATGGATTTAAGTTGGAAAGA
AACTATTCGTCCACTCTTTAAATATTTCACTCTTCGTACTCCAGGTTCTTTCTTTGAAGA
AAAAGAAATGTTATTCACATGGCATTATAGAAATGCTGATCCAATATTTGGTTCAATTCA
AGCACGTGAATTACATTTACATTTAGATAATTTACCTTTAGATGTTATAGTTGGTGATAA
AACATTGGGTGTAAGATCATATAATATAAATCCATTATCCTCAATGAAAAAAGTTATAAC
AGACACAATTCCAAAAGGATTAGATTTAATTTTATTAATTGGTGATACTCATATTCATCC
AAGTGAATTGCCAACATTTGATGGTAAAATCTTTAATATTTCAGTTGGTAAAAAATCTGT
TAAAGATAGTTACCATTTAAGTGATCCAGCTGAAGTTAATTATCTTTTAAATAGATTAGA
AAAAATTTCAAAAGATGAATCATGTGCTCCTGTACCTGATAATTAATTTGTTTATCAAAA
TAAAAATAAAAATTATAAATAGAATAAATAAATAAATTAATAATAAAA