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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLI232
Library SL
(Link to library)
Clone ID SLI232
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15735-1
Original site URL
Representative seq. ID SLI232E
(Link to Original site)
Representative DNA sequence 
>SLI232 (SLI232Q) /CSM/SL/SLI2-B/SLI232Q.Seq.d/
CATGTATCAGCTGAAGATAAAGCTTCTGGTTCTAAAGAATCCATCACCATTACCAACGAT
AAGGAACGTCTCTCACAAGCCGATATCGATCGTATGGTCAAAGAAGCTGCCGATGCCGCT
GATGAAGATAAAGCTGCCAAAGAACGTGTCGAAGCCAAGAATACCTTGGAAAAATTACAT
CTATCAAATTAAAAACACCATTGGTGATAAAGATAAGATCGGTGGTAAAATCGAATCAGA
TGATAAAGAAACCATTGAAACCGCTATCTCTGATGCCCTTTCTTGGTTGGATGTTAACTC
CTCTGCCGAAAAAGAAGAATTTGATGACCAATACAAAATCCTTGAAAAGATCGTTCAACC
AATCTTCTCTAAATTATATGGTGCTGCTGGTGGTTCCCCAAATGGCGCTGGTGAAGATAT
GCCAAACCATGATGAACTTTAAACGCAATTTTCAAATTTTTTAAAAAAAAAAGTATAGAT
TTTAAAAAAAAAATAAAAAATAAAATAAAAAAAAAAAATATAAAAC
sequence update 1998. 8.22
Translated Amino Acid sequence 
MYQLKIKLLVLKNPSPLPTIRNVSHKPISIVWSKKLPMPLMKIKLPKNVSKPRIPWKNYI
YQIKNTIGDKDKIGGKIESDDKETIETAISDALSWLDVNSSAEKEEFDDQYKILEKIVQP
IFSKLYGAAGGSPNGAGEDMPNHDEL*tqfsnflkkkv*ilkkk*kik*kkki*n
Translated Amino Acid sequence (All Frames) 
Frame A:
hvsaedkasgskesititndkerlsqadidrmvkeaadaadedkaakerveakntleklh
lsn*khhw**r*drw*nrir**rnh*nryl*cpflvgc*llcrkrri**piqnp*kdrst
nll*iiwccwwfpkwrw*ryakp**tlnaifkffkkksidfkkkiknkikkknik


Frame B:
MYQLKIKLLVLKNPSPLPTIRNVSHKPISIVWSKKLPMPLMKIKLPKNVSKPRIPWKNYI
YQIKNTIGDKDKIGGKIESDDKETIETAISDALSWLDVNSSAEKEEFDDQYKILEKIVQP
IFSKLYGAAGGSPNGAGEDMPNHDEL*tqfsnflkkkv*ilkkk*kik*kkki*n


Frame C:
cis*r*sfwf*rihhhyqr*gtsltsryrsygqrscrcr**r*scqrtcrsqeylgkits
iklktplvikirsvvksnqmikkplkplslmpflgwmltplpkkknlmtntkslkrsfnq
sslnymvllvvpqmalvkicqtmmnfkrnfqif*kkkyrf*kknkk*nkkkkyk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLI232 (SLI232Q) /CSM/SL/SLI2-B/SLI232Q.Seq.d/                        916   0.0
VSC175 (VSC175Q) /CSM/VS/VSC1-D/VSC175Q.Seq.d/                        892   0.0
AFM366 (AFM366Q) /CSM/AF/AFM3-C/AFM366Q.Seq.d/                        892   0.0
AFM728 (AFM728Q) /CSM/AF/AFM7-B/AFM728Q.Seq.d/                        886   0.0
AFM580 (AFM580Q) /CSM/AF/AFM5-D/AFM580Q.Seq.d/                        886   0.0
AFD384 (AFD384Q) /CSM/AF/AFD3-D/AFD384Q.Seq.d/                        878   0.0
FCL-AD17 (FCL-AD17Q) /CSM/FCL/FCL-AD/FCL-AD17Q.Seq.d/                 876   0.0
AFF462 (AFF462Q) /CSM/AF/AFF4-C/AFF462Q.Seq.d/                        870   0.0
SLF229 (SLF229Q) /CSM/SL/SLF2-B/SLF229Q.Seq.d/                        866   0.0
AFE593 (AFE593Q) /CSM/AF/AFE5-D/AFE593Q.Seq.d/                        864   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU053251) Dictyostelium discoideum slug cDNA, clone SLI232.      916   0.0    1
(AC114263) Dictyostelium discoideum chromosome 2 map 215673-...   571   0.0    2
(BJ346917) Dictyostelium discoideum cDNA clone:dda25d17, 3' ...   571   0.0    2
(AU262379) Dictyostelium discoideum vegetative cDNA clone:VS...   571   0.0    2
(BJ347176) Dictyostelium discoideum cDNA clone:dda26h07, 3' ...   571   0.0    2
(BJ347384) Dictyostelium discoideum cDNA clone:dda26o19, 3' ...   571   0.0    2
(BJ341709) Dictyostelium discoideum cDNA clone:dda7h21, 3' e...   563   0.0    2
(AU052865) Dictyostelium discoideum slug cDNA, clone SLF229.      571   0.0    2
(BJ340102) Dictyostelium discoideum cDNA clone:dda11l16, 3' ...   511   0.0    3
(BJ339898) Dictyostelium discoideum cDNA clone:dda10i23, 3' ...   511   0.0    3
dna update 2009. 6.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9LKR3) RecName: Full=Luminal-binding protein 1;          Short...    96   7e-29
D89341_1(D89341|pid:none) Arabidopsis thaliana DNA for luminal b...    96   7e-29
(P49118) RecName: Full=Luminal-binding protein;          Short=B...    98   1e-28
BT045516_1(BT045516|pid:none) Salmo salar clone ssal-rgf-523-306...    99   2e-28
AB196459_1(AB196459|pid:none) Oncorhynchus mykiss GRP78 mRNA for...    98   2e-28
DQ662232_1(DQ662232|pid:none) Paralichthys olivaceus glucose-reg...    98   3e-28
AY137471_1(AY137471|pid:none) Panagrellus redivivus heat shock p...    94   6e-28
D84414_1(D84414|pid:none) Arabidopsis thaliana mRNA for luminal ...    92   7e-28
D89342_1(D89342|pid:none) Arabidopsis thaliana DNA for luminal b...    92   7e-28
(Q39043) RecName: Full=Luminal-binding protein 2;          Short...    92   7e-28
protein update 2009. 7. 5
PSORT 

 psg: 0.73  gvh: 0.37  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.10
 nuc: 0.00  erl: 1.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for SLI232 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID SLI232E
Full length Seq. 
>SLI232E.Seq
CATGTATCAGCTGAAGATAAAGCTTCTGGTTCTAAAGAATCCATCACCATTACCAACGAT
AAGGAACGTCTCTCACAAGCCGATATCGATCGTATGGTCAAAGAAGCTGCCGATGCCGCT
GATGAAGATAAAGCTGCCAAAGAACGTGTCGAAGCCAAGAATACCTTGGAAAAATTACAT
CTATCAAATTAAAAACACCATTGGTGATAAAGATAAGATCGGTGGTAAAATCGAATCAGA
TGATAAAGAAACCATTGAAACCGCTATCTCTGATGCCCTTTCTTGGTTGGATGTTAACTC
CTCTGCCGAAAAAGAAGAATTTGATGACCAATACAAAATCCTTGAAAAGATCGTTCAACC
AATCTTCTCTAAATTATATGGTGCTGCTGGTGGTTCCCCAAATGGCGCTGGTGAAGATAT
GCCAAACCATGATGAACTTTAAACGCAATTTTCAAATTTTTTAAAAAAAAAAGTATAGAT
TTTAAAAAAAAAATAAAAAATAAAATAAAAAAAAAAAATATAAAAC
Length of full length seq. 526