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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK520
Library AF
(Link to library)
Clone ID AFK520
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16444-1
Original site URL
Representative seq. ID AFK520Z
(Link to Original site)
Representative DNA sequence 
>AFK520 (AFK520Q) /CSM/AF/AFK5-A/AFK520Q.Seq.d/
XXXXXXXXXXCGCTTCAGATCCATTATCAAATATCACCGAGCCAGTTGGCCTCAACTTAA
TAGATCATGTCGTTTCAAATCATGCAGATAGAATGATGGAACCAGTCGTTCAATGGTACG
AAAAGGTTTTACAATTCCACCGTTTCTGGTCAGTTGATGACAAAACCATTCATACCGAAT
ATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAAAAGTTAAATTACCAATTA
ATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAATACGTAGATTTCTACAATG
GTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCATTGATGCTATCTCAAAAT
TAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACATACTATACATCACTCAGAG
AGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGGACACTTTAGAGAAATTAC
ACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAATCTTTACAAATAATGTTG
AAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACAACCATGATGGTTTCGGTG
CTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAACAAGAAACTCGTGGAAACT
TATAGGTGTGTCTCAATTACAAATGTTCTTTATTATTAACTAAATAACATTACAATATAT
CAAAATAAATAATAAATAAAACTCATTTAAAAAGAATTTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---ASDPLSNITEPVGLNLIDHVVSNHADRMMEPVVQWYEKVLQFHRFWSVDDKTIHTEY
SSLRSVVVADKSEKVKLPINEPANGIRKSQIQEYVDFYNGAGVQHIALKTDNIIDAISKL
RSRGVSFLTVPKTYYTSLREKLQHSSLEIKEDLDTLEKLHILIDYDDKGYLLQIFTNNVE
DKPTVFFEIIQRNNHDGFGAGNFKSLFEAIERQQETRGNL*vclnykcsllltk*hynis
k*iinkthlkrif
Translated Amino Acid sequence (All Frames) 
Frame A:
---ASDPLSNITEPVGLNLIDHVVSNHADRMMEPVVQWYEKVLQFHRFWSVDDKTIHTEY
SSLRSVVVADKSEKVKLPINEPANGIRKSQIQEYVDFYNGAGVQHIALKTDNIIDAISKL
RSRGVSFLTVPKTYYTSLREKLQHSSLEIKEDLDTLEKLHILIDYDDKGYLLQIFTNNVE
DKPTVFFEIIQRNNHDGFGAGNFKSLFEAIERQQETRGNL*vclnykcsllltk*hynis
k*iinkthlkrif


Frame B:
---lqihyqispsqlast**imsfqimqie*wnqsfngtkrfynstvsgqlmtkpfipni
hh*dqs*llislkklnyqlmnqpmvlervkfknt*istmvlvfnisp*rlitslmlsqn*
dlvvslsslfqkhtihhsernyntlh*klkkiwtl*rnytf*simmtkvifykslqimlk
inqlfslklskettmmvsvlvtlnpslkqskdnkklvetyrcvsitnvlyy*lnnitiyq
nk**ikli*kef


Frame C:
---frsiikyhraswpqlnrscrfkscr*ndgtsrsmvrkgftippflvs**qnhsyrif
iikisrsc**v*ks*itn**tsqwy*kesnsrirrflqwcwcstyrlkd**hh*cylkik
iswclfphcsknilyitqreittlfirn*rrfghfreithfnrl**qrlsftnlyk*c*r
*tncfl*nypkkqp*wfrcw*l*ipl*snrkttrnswkligvsqlqmffiin*itlqyik
innk*nsfkknf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK520 (AFK520Q) /CSM/AF/AFK5-A/AFK520Q.Seq.d/                       1489   0.0
SFI803 (SFI803Q) /CSM/SF/SFI8-A/SFI803Q.Seq.d/                       1465   0.0
SFG320 (SFG320Q) /CSM/SF/SFG3-A/SFG320Q.Seq.d/                       1461   0.0
AFG569 (AFG569Q) /CSM/AF/AFG5-C/AFG569Q.Seq.d/                       1461   0.0
VFG192 (VFG192Q) /CSM/VF/VFG1-D/VFG192Q.Seq.d/                       1459   0.0
VFF690 (VFF690Q) /CSM/VF/VFF6-D/VFF690Q.Seq.d/                       1459   0.0
CFF120 (CFF120Q) /CSM/CF/CFF1-A/CFF120Q.Seq.d/                       1459   0.0
SFE536 (SFE536Q) /CSM/SF/SFE5-B/SFE536Q.Seq.d/                       1455   0.0
CFC563 (CFC563Q) /CSM/CF/CFC5-C/CFC563Q.Seq.d/                       1453   0.0
AFM586 (AFM586Q) /CSM/AF/AFM5-D/AFM586Q.Seq.d/                       1453   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.  1431   0.0    12
BI322343|BI322343.1 kx19h02.y3 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    42   5e-10  4
BI502176|BI502176.1 kt86c08.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    36   1e-09  5
CO006156|CO006156.1 EST794491 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAT15 3' end, mRNA sequence.    52   5e-08  2
CO013595|CO013595.1 EST801930 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBZ67 5' end, mRNA sequence.    52   7e-08  2
CO005676|CO005676.1 EST794011 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAQ39 3' end, mRNA sequence.    52   7e-08  2
CO009511|CO009511.1 EST797846 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBC24 5' end, mRNA sequence.    52   7e-08  2
CO009510|CO009510.1 EST797845 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBC24 3' end, mRNA sequence.    52   8e-08  2
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          44   1e-07  20
L38493|L38493.1 Coccidioides immitis T-cell reactive protein (trcP) gene exons 1-4, complete cds.    52   2e-07  2
dna update 2004. 8. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NV5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   435   e-121
AM114773_1(AM114773|pid:none) Platynereis dumerilii partial mRNA...   298   1e-79
AE014296_3563(AE014296|pid:none) Drosophila melanogaster chromos...   292   6e-78
S32821(S32821;S35890;S35889)4-hydroxyphenylpyruvate dioxygenase ...   288   9e-77
(Q02110) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   285   1e-75
BC046075_1(BC046075|pid:none) Danio rerio zgc:56326, mRNA (cDNA ...   284   2e-75
(Q6TGZ5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   284   2e-75
BC153801_1(BC153801|pid:none) Xenopus laevis hypothetical protei...   282   7e-75
(P32754) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   281   1e-74
BC077167_1(BC077167|pid:none) Danio rerio 4-hydroxyphenylpyruvat...   280   3e-74
protein update 2009. 4.24
PSORT 

 psg: 0.75  gvh: 0.24  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for AFK520 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFK520Z
3' end seq. 
>AFK520Z.Seq
----------CGCTTCAGATCCATTATCAAATATCACCGAGCCAGTTGGCCTCAACTTAA
TAGATCATGTCGTTTCAAATCATGCAGATAGAATGATGGAACCAGTCGTTCAATGGTACG
AAAAGGTTTTACAATTCCACCGTTTCTGGTCAGTTGATGACAAAACCATTCATACCGAAT
ATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAAAAGTTAAATTACCAATTA
ATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAATACGTAGATTTCTACAATG
GTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCATTGATGCTATCTCAAAAT
TAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACATACTATACATCACTCAGAG
AGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGGACACTTTAGAGAAATTAC
ACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAATCTTTACAAATAATGTTG
AAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACAACCATGATGGTTTCGGTG
CTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAACAAGAAACTCGTGGAAACT
TATAGGTGTGTCTCAATTACAAATGTTCTTTATTATTAACTAAATAACATTACAATATAT
CAAAATAAATAATAAATAAAACTCATTTAAAAAGAATTTTT
Length of 3' end seq. 751
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -