>AFK519 (AFK519Q) /CSM/AF/AFK5-A/AFK519Q.Seq.d/
AAATTATTTCCCACCCAAAAGCACACCATAACATTGTATTTATATATAAATATTATCACA
CAAATTATTAAATTATTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAATA
AAAATAATAATAACCGAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAATA
GATTTAAAAAAAAAATATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAGC
AAGAAGAGATGCTGAATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATGA
TACTACTCTCAAAACTTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAAT
TAAAGTTAGAAGAAACCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTGA
AGATAATGTTCATTTAGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTTT
AACTACCAACAAAGTCCACGCCATTCCATTAAGATCATCATGGGTTATGACATGTGCTTA
TTCACCAACTGCTAACXXXXXXXXXXGATATGACATGTATTTTATGGGATGTTGAAAATG
GTACAAAGATCACAGAATTCTCTGACCACAACGGGTGATGTCATGAGTGTCAGTGTTTCA
CCAGATAAAAACTATTTCATCAGTGGTGCTTGTGATGCTACCGCCAAATTATGGGATTTA
CGTAGTGGTAAATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAA
TATTTCCCAAATGGTTTATCATTTGGTACTGGTTCTGATGATGCTTCATGTAGATTATTT
GATATTCGTGCCGATCGTGAATTAATGCAATACACCCACGACAACATTTTATGTGGTATT
ACATCCGTTGGTTTTTCATTTTCAGGTCGTTTCTTATTTGCTGGTTACGATGATTTCACT
TGCAACGTTTGGGATACATTAAAGGGTGAACGTGTTCTCTCTTTAACTGGTCACGGTAAT
CGTGTATCTTGTCTTGGTGTACCAACCGATGGTATGGCTTTATGTACAGGTAGTTGGGAT
TCTCTCCTCAAGATTTGGGCTTAAATTTTTTAATAATATTAACATAATAATAATAATAAT
AATAATAATAATAATTATAAAAATAACAAAAATAATATCAACAATACTATCAAATTTCAC
CTCACTCATTTAAAATTCACATCAACAACACACGCAACTTCTCACACAACCATTAATTAT
GTCGTTTTCCCCAGTATCTTTCCAACAATTAATTGTCCTTGTATAATATTATAAACAATT
ACAACCC

nyfppkstp*hciyi*ilshkllnyy*yi*lfriik****k***pnfkinkn*ttiiyk*
i*kkni*KMSSDISEKIQQARRDAESMKEQIRANRDVMNDTTLKTFTRDLPGLPKMEGKI
KVRRNLKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAY
SPTAN---

---DMTCILWDVENGTKITEFSDHNG*checqcftr*klfhqwcl*cyrqimgft*w*mc
snfhwsrs*y*cssifpkwfiiwywf**cfm*ii*yscrs*inaihprqhfmwyyirwff
ifrsflicwlr*fhlqrlgyikg*tcslfnwsr*scilswctnrwygfmyr*lgfspqdl
glnfliilt**********l*k*qk*yqqyyqisphsfkihinntrnfshnh*lcrfpqy
lsnn*lslyniinnyn

Frame A:
klfptqkhtitlylyiniitqiiklllvyiii*nnkiiiikiiitef*n**klnnyni*i
dlkkkyiknviryfrknstskkrc*inertn*skqrcne*yysqnfys*ftrsskdgr*n
*s*kkp*gssc*nlcyalg*r*csfsfclsrw*iislgwfnyqqsprhsikiimgydmcl
ftnc*---

---DMTCILWDVENGTKITEFSDHNG*checqcftr*klfhqwcl*cyrqimgft*w*mc
snfhwsrs*y*cssifpkwfiiwywf**cfm*ii*yscrs*inaihprqhfmwyyirwff
ifrsflicwlr*fhlqrlgyikg*tcslfnwsr*scilswctnrwygfmyr*lgfspqdl
glnfliilt**********l*k*qk*yqqyyqisphsfkihinntrnfshnh*lcrfpqy
lsnn*lslyniinnyn

Frame B:
nyfppkstp*hciyi*ilshkllnyy*yi*lfriik****k***pnfkinkn*ttiiyk*
i*kkni*KMSSDISEKIQQARRDAESMKEQIRANRDVMNDTTLKTFTRDLPGLPKMEGKI
KVRRNLKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAY
SPTAN---

---i*hvfygmlkmvqrsqnsltttgdvmsvsvspdknyfisgacdataklwdlrsgkcv
qtftgheadinavqyfpnglsfgtgsddascrlfdiradrelmqythdnilcgitsvgfs
fsgrflfagyddftcnvwdtlkgervlsltghgnrvsclgvptdgmalctgswdsllkiw
a*if**y*hnnnnnnnnnnyknnknninntikfhlthlkftstthatshttinyvvfpsi
fptincpciil*titt

Frame C:
iishpkahhnivfiykyyhtny*iitsiynyle**nnnnknnnnrilkliktkql*yinr
fkkkiykkchqifqkkfnkqeemlnq*knkleqtem**millsklllviyqvfqrwkvkl
kleetlrvillksmlciglkimfi*flplkmvny*fgmv*lptkstpfh*dhhgl*hvli
hqll---

---ydmyfmgc*kwykdhril*pqrvms*vsvfhqiktissvvlvmlppnygiyvvvnvf
klslvtklilmqfnisqmvyhlvlvlmmlhvdylifvpivn*cntptttfyvvlhplvfh
fqvvsyllvtmislatfgih*rvnvfsl*lvtvivylvlvyqpmvwlyvqvvgilssrfg
lkffnniniiiiiiiiiiiikitkiistilsnftsli*nshqqhtqlltqplimsfspvs
fqqlivlv*yykqlqp

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK519 (AFK519Q) /CSM/AF/AFK5-A/AFK519Q.Seq.d/                       1772   0.0
AFJ370 (AFJ370Q) /CSM/AF/AFJ3-C/AFJ370Q.Seq.d/                       1063   0.0
VFK342 (VFK342Q) /CSM/VF/VFK3-B/VFK342Q.Seq.d/                       1027   0.0
AFO321 (AFO321Q) /CSM/AF/AFO3-A/AFO321Q.Seq.d/                        862   0.0
SSD702 (SSD702Q) /CSM/SS/SSD7-A/SSD702Q.Seq.d/                        781   0.0
SLA519 (SLA519Q) /CSM/SL/SLA5-A/SLA519Q.Seq.d/                        726   0.0
AFO746 (AFO746Q) /CSM/AF/AFO7-B/AFO746Q.Seq.d/                        726   0.0
AFM380 (AFM380Q) /CSM/AF/AFM3-D/AFM380Q.Seq.d/                        726   0.0
SFB734 (SFB734Q) /CSM/SF/SFB7-B/SFB734Q.Seq.d/                        718   0.0
SLD136 (SLD136Q) /CSM/SL/SLD1-B/SLD136Q.Seq.d/                        712   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X73641|X73641.1 Dictyostelium discoideum gene for G-protein beta-subunit.   934   0.0    2
Z23105|Z23105.1 L.stagnalis mRNA for G protein beta subunit.       52   1e-11  2
AY401713|AY401713.1 Pan troglodytes HCM0982 gene, VIRTUAL TRANSCRIPT, partial sequence, genomic survey sequence.    50   7e-11  4
BM475096|BM475096.1 AGENCOURT_6477641 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:5559167 5', mRNA sequence.    50   2e-10  5
AY661671|AY661671.1 Lentinula edodes guanine nucleotide binding protein beta subunit 2 (Gbeta2) mRNA, complete cds.    52   2e-10  4
AY401712|AY401712.1 Homo sapiens HCM0982 gene, VIRTUAL TRANSCRIPT, partial sequence, genomic survey sequence.    50   3e-10  5
AF300648|AF300648.1 Homo sapiens guanine nucleotide binding protein beta subunit 4 (GNB4) mRNA, complete cds.    50   3e-10  5
BC000873|BC000873.1 Homo sapiens, guanine nucleotide binding protein beta subunit 4, clone MGC:5262, mRNA, complete cds.    50   6e-10  5
CR591019|CR591019.1 full-length cDNA clone CS0DI009YC19 of Placenta Cot 25-normalized of Homo sapiens (human).    50   6e-09  5
CR563913|CR563913.1 Xenopus tropicalis EST, clone THdA004c22 5'.    40   7e-09  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36408) RecName: Full=Guanine nucleotide-binding protein subuni...   331   e-100
AY661671_1(AY661671|pid:none) Lentinula edodes guanine nucleotid...   271   1e-78
AF536325_1(AF536325|pid:none) Ustilago maydis G-protein beta sub...   267   5e-78
AF091120_1(AF091120|pid:none) Filobasidiella neoformans G-protei...   265   1e-76
AF056182_1(AF056182|pid:none) Emericella nidulans G-protein beta...   250   2e-72
DQ863323_1(DQ863323|pid:none) Penicillium marneffei STE4-like pr...   249   3e-72
AY550249_1(AY550249|pid:none) Paracoccidioides brasiliensis smal...   249   4e-72
AB188197_1(AB188197|pid:none) Botryotinia fuckeliana bcgb1 gene ...   246   1e-70
(P17343) RecName: Full=Guanine nucleotide-binding protein subuni...   251   2e-70
EU586204_1(EU586204|pid:none) Meloidogyne javanica G-protein bet...   249   2e-70

 psg: 0.38  gvh: 0.30  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK519 is nuc

>AFK519F.Seq
AAATTATTTCCCACCCAAAAGCACACCATAACATTGTATTTATATATAAATATTATCACA
CAAATTATTAAATTATTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAATA
AAAATAATAATAACCGAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAATA
GATTTAAAAAAAAAATATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAGC
AAGAAGAGATGCTGAATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATGA
TACTACTCTCAAAACTTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAAT
TAAAGTTAGAAGAAACCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTGA
AGATAATGTTCATTTAGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTTT
AACTACCAACAAAGTCCACGCCATTCCATTAAGATCATCATGGGTTATGACATGTGCTTA
TTCACCAACTGCTAAC----------

>AFK519Z.Seq
----------GATATGACATGTATTTTATGGGATGTTGAAAATGGTACAAAGATCACAGA
ATTCTCTGACCACAACGGGTGATGTCATGAGTGTCAGTGTTTCACCAGATAAAAACTATT
TCATCAGTGGTGCTTGTGATGCTACCGCCAAATTATGGGATTTACGTAGTGGTAAATGTG
TTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAATATTTCCCAAATGGTT
TATCATTTGGTACTGGTTCTGATGATGCTTCATGTAGATTATTTGATATTCGTGCCGATC
GTGAATTAATGCAATACACCCACGACAACATTTTATGTGGTATTACATCCGTTGGTTTTT
CATTTTCAGGTCGTTTCTTATTTGCTGGTTACGATGATTTCACTTGCAACGTTTGGGATA
CATTAAAGGGTGAACGTGTTCTCTCTTTAACTGGTCACGGTAATCGTGTATCTTGTCTTG
GTGTACCAACCGATGGTATGGCTTTATGTACAGGTAGTTGGGATTCTCTCCTCAAGATTT
GGGCTTAAATTTTTTAATAATATTAACATAATAATAATAATAATAATAATAATAATAATT
ATAAAAATAACAAAAATAATATCAACAATACTATCAAATTTCACCTCACTCATTTAAAAT
TCACATCAACAACACACGCAACTTCTCACACAACCATTAATTATGTCGTTTTCCCCAGTA
TCTTTCCAACAATTAATTGTCCTTGTATAATATTATAAACAATTACAACCC

>AFK519P.Seq
AAATTATTTCCCACCCAAAAGCACACCATAACATTGTATTTATATATAAATATTATCACA
CAAATTATTAAATTATTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAATA
AAAATAATAATAACCGAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAATA
GATTTAAAAAAAAAATATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAGC
AAGAAGAGATGCTGAATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATGA
TACTACTCTCAAAACTTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAAT
TAAAGTTAGAAGAAACCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTGA
AGATAATGTTCATTTAGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTTT
AACTACCAACAAAGTCCACGCCATTCCATTAAGATCATCATGGGTTATGACATGTGCTTA
TTCACCAACTGCTAAC----------GATATGACATGTATTTTATGGGATGTTGAAAATG
GTACAAAGATCACAGAATTCTCTGACCACAACGGGTGATGTCATGAGTGTCAGTGTTTCA
CCAGATAAAAACTATTTCATCAGTGGTGCTTGTGATGCTACCGCCAAATTATGGGATTTA
CGTAGTGGTAAATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAA
TATTTCCCAAATGGTTTATCATTTGGTACTGGTTCTGATGATGCTTCATGTAGATTATTT
GATATTCGTGCCGATCGTGAATTAATGCAATACACCCACGACAACATTTTATGTGGTATT
ACATCCGTTGGTTTTTCATTTTCAGGTCGTTTCTTATTTGCTGGTTACGATGATTTCACT
TGCAACGTTTGGGATACATTAAAGGGTGAACGTGTTCTCTCTTTAACTGGTCACGGTAAT
CGTGTATCTTGTCTTGGTGTACCAACCGATGGTATGGCTTTATGTACAGGTAGTTGGGAT
TCTCTCCTCAAGATTTGGGCTTAAATTTTTTAATAATATTAACATAATAATAATAATAAT
AATAATAATAATAATTATAAAAATAACAAAAATAATATCAACAATACTATCAAATTTCAC
CTCACTCATTTAAAATTCACATCAACAACACACGCAACTTCTCACACAACCATTAATTAT
GTCGTTTTCCCCAGTATCTTTCCAACAATTAATTGTCCTTGTATAATATTATAAACAATT
ACAACCC