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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK518
Library AF
(Link to library)
Clone ID AFK518
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13846-1
Original site URL
Representative seq. ID AFK518P
(Link to Original site)
Representative DNA sequence 
>AFK518 (AFK518Q) /CSM/AF/AFK5-A/AFK518Q.Seq.d/
AATATTTCTTTCTCTCTCTCAACACATTAAAATTTTTTTAAACACGTTAAATAAAAAAAA
TGATTAAAAAATTAATATTAGATCCAAAGAATGTATCAATAATTAAAAATGGTATTAGAA
ATTATTCAAAATCAAAATCATTTATTCAACCAATTACAACATTATCAGAAGAGGAAACTT
TATTAAAAGAAACTGTTGCAAATTTTGCAAATGAAAAAGTTAGACCATTAGTAAAGGTTA
TGGATGAAACATCAGAATTAAATAAAGGATTATTAAAAGATTTATTCGATATGAATTTAA
TGGGTATTGATATTTCAGATAGTTATGGTGGAGCCAATATGAATTTTATGGGATCAATCA
TTGCTATTGAAGAATTAGCAAAAGTTGATCCAGCCATTAGTGTAATAGTAGATGTACAAA
ATACACTCGTTAACAATTGTATCAATCGTTATGGTAGCATACAACAAAGAGAAAAGTATT
TATCAATGTTGGCAACCAACACAGTTGGTAGTTTTTGTCTAAGTGAATCAGGCCTCAGGT
TCTGATGCATTCGCATTGGCAACTCGTGCAGTCAGAXXXXXXXXXXCGTGCAGTCAGACA
ATCAGATGGCACATTCGTTTTAAATGGCACTAACCAATGGATCACAAATGCCAAAGAGGC
AGGTGTTTTCATCGTTATGGCAAATGTCGACCCATCACAGGGTTACAAAGGTATCACTGC
CTTCATTGTTGAATCTAATAATCCAGGTTTAAGAATTGGTAAGAAGGAGGATAAATTAGG
TATTAGAGCAAGCAGTACTTGTGAAGTTATTTTGGACAATTGTGTGGTAAAACCAACTGA
TATCCTCGGTGAATTGGGTAGAGGTTATAAAATTGCTATCGAGGGTTTAAATGAAGGTCG
TATTGGTATCGCTGCTCAAATGTTGGGTTTAGCTCAAGGTGTATTCGATTCAACTATTCC
TTACTTGATGGAACGTAAACAATTTGGTAAACCAATCGCTACTTTCCAAGGTATGCAATT
CACTTATGCAGATTTAGCTGTAGATATTGAAGCTGGTAGACTTTTAACTTATAATGCAGC
TCGTATTAAAGAAGCTGGTCTACCATTTGTTTTCCAAGCTTCAATGGCAAAACTTCATTG
TTCACGTGTCGCTGAAAAAGCAGCTTCTGCTTGTATTTCAATGTTGGGTGGTGTTGGTTT
CACTAAAGAATTCCCTGCTGAAAAATTCTTTAGAGATTCAAAAGTTGGTCAAATTTATGA
AGGTACTTCAAATATTCAATTACAAACAATGCAAAAGAAATGTTAAAAATTTAATAAATA
AAATAAANAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nisfslsth*nffkhvk*kk*lkn*y*iqrmyq*lkmvleiiqnqnhlfnqlqhyqkrkl
y*kkllqilqmkkldh**rlwmkhqn*ikdy*kiysi*i*wvlifqivmvepi*ilwdqs
lllkn*qkliqplv***mykihsltivsivmvaynkeksiyqcwqptqlvvfv*VNQASG
SDAFALATRAVR---

---RAVRQSDGTFVLNGTNQWITNAKEAGVFIVMANVDPSQGYKGITAFIVESNNPGLRI
GKKEDKLGIRASSTCEVILDNCVVKPTDILGELGRGYKIAIEGLNEGRIGIAAQMLGLAQ
GVFDSTIPYLMERKQFGKPIATFQGMQFTYADLAVDIEAGRLLTYNAARIKEAGLPFVFQ
ASMAKLHCSRVAEKAASACISMLGGVGFTKEFPAEKFFRDSKVGQIYEGTSNIQLQTMQK
KC*kfnk*nkx
Translated Amino Acid sequence (All Frames) 
Frame A:
nisfslsth*nffkhvk*kk*lkn*y*iqrmyq*lkmvleiiqnqnhlfnqlqhyqkrkl
y*kkllqilqmkkldh**rlwmkhqn*ikdy*kiysi*i*wvlifqivmvepi*ilwdqs
lllkn*qkliqplv***mykihsltivsivmvaynkeksiyqcwqptqlvvfv*VNQASG
SDAFALATRAVR---

---RAVRQSDGTFVLNGTNQWITNAKEAGVFIVMANVDPSQGYKGITAFIVESNNPGLRI
GKKEDKLGIRASSTCEVILDNCVVKPTDILGELGRGYKIAIEGLNEGRIGIAAQMLGLAQ
GVFDSTIPYLMERKQFGKPIATFQGMQFTYADLAVDIEAGRLLTYNAARIKEAGLPFVFQ
ASMAKLHCSRVAEKAASACISMLGGVGFTKEFPAEKFFRDSKVGQIYEGTSNIQLQTMQK
KC*kfnk*nkx

Frame B:
iflslsqhikiflntlnkknd*kinirskecinn*kwy*klfkikiiystnyniirrgnf
ikrncckfck*ks*tiskgyg*nirik*riikrfiryefngy*yfr*lwwsqyefyginh
cy*risks*ssh*cnsrctkytr*qlyqslw*httkrkvfinvgnqhsw*flsk*irpqv
lmhshwqlvqs---

---vqsdnqmahsf*maltngsqmpkrqvfsslwqmsthhrvtkvslpsllnliiqv*el
vrrrin*vleqavlvklfwtivw*nqlissvnwvevikllsrv*mkvvlvsllkcwv*lk
vysiqlflt*wnvnnlvnqsllskvcnslmqi*l*ilklvdf*limqlvlkklvyhlfsk
lqwqnfivhvslkkqlllvfqcwvvlvslknsllknsleiqklvkfmkvlqifnykqckr
nvknlinkix

Frame C:
yfflslntlkff*tr*ikkmikklildpknvsiikngirnysksksfiqpittlseeetl
lketvanfanekvrplvkvmdetselnkgllkdlfdmnlmgidisdsygganmnfmgsii
aieelakvdpaisvivdvqntlvnncinrygsiqqrekylsmlatntvgsfclsesglrf
*cirignscsq---

---csqtirwhirfkwh*pmdhkcqrgrcfhrygkcrpitglqryhclhc*i**srfknw
*egg*iry*skqyl*syfgqlcgktn*ypr*ig*rl*ncyrgfk*rsywyrcsnvgfssr
cirfnyslldgt*tiw*tnryfpryaihlcrfscry*sw*tfnl*cssy*rswsticfps
fngktslftcr*kssfclyfnvgwcwfh*ripc*kil*rfkswsnl*ryfkysitnnake
mlki**ik*x
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK518 (AFK518Q) /CSM/AF/AFK5-A/AFK518Q.Seq.d/                       2454   0.0
SLG556 (SLG556Q) /CSM/SL/SLG5-C/SLG556Q.Seq.d/                       1388   0.0
SFC345 (SFC345Q) /CSM/SF/SFC3-B/SFC345Q.Seq.d/                       1388   0.0
AFG654 (AFG654Q) /CSM/AF/AFG6-C/AFG654Q.Seq.d/                       1388   0.0
AFD462 (AFD462Q) /CSM/AF/AFD4-C/AFD462Q.Seq.d/                       1388   0.0
AFA590 (AFA590Q) /CSM/AF/AFA5-D/AFA590Q.Seq.d/                       1388   0.0
AFF278 (AFF278Q) /CSM/AF/AFF2-D/AFF278Q.Seq.d/                       1382   0.0
AFD724 (AFD724Q) /CSM/AF/AFD7-A/AFD724Q.Seq.d/                       1362   0.0
AFO692 (AFO692Q) /CSM/AF/AFO6-D/AFO692Q.Seq.d/                       1358   0.0
SFK863 (SFK863Q) /CSM/SF/SFK8-C/SFK863Q.Seq.d/                       1346   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AU114157|AU114157.1 Caenorhabditis elegans cDNA clone:yk715h10 : 3' end, single read.    54   6e-10  2
BJ780526|BJ780526.1 Caenorhabditis elegans cDNA clone:yk1424d10 : 3' end.    48   3e-08  2
D72651|D72651.1 C.elegans cDNA clone yk106c6 : 3' end, single read.    48   3e-08  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   9e-08  19
CO004166|CO004166.1 EST792501 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAH80 3' end, mRNA sequence.    70   1e-07  1
CO006553|CO006553.1 EST794888 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAV43 3' end, mRNA sequence.    70   1e-07  1
CO003886|CO003886.1 EST792221 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAG23 3' end, mRNA sequence.    70   1e-07  1
CO011999|CO011999.1 EST800334 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBQ42 3' end, mRNA sequence.    70   1e-07  1
CO004167|CO004167.1 EST792502 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAH80 5' end, mRNA sequence.    70   1e-07  1
AC006625|AC006625.1 Caenorhabditis elegans clone C55B7, complete sequence.    46   1e-07  2
dna update 2004. 8. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RR5) RecName: Full=Probable short/branched chain specific ac...   461   0.0
CP000473_5244(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   294   e-106
AB169822_1(AB169822|pid:none) Macaca fascicularis brain cDNA, cl...   288   e-105
AB170478_1(AB170478|pid:none) Macaca fascicularis brain cDNA clo...   288   e-105
DQ864937_1(DQ864937|pid:none) Pfiesteria piscicida clone ppi-5p-...   294   e-105
AL831821_1(AL831821|pid:none) Homo sapiens mRNA; cDNA DKFZp451K2...   288   e-105
CP000360_1129(CP000360|pid:none) Acidobacteria bacterium Ellin34...   295   e-105
CR762047_1(CR762047|pid:none) Xenopus tropicalis finished cDNA, ...   279   e-103
AF039038_2(AF039038|pid:none) Caenorhabditis elegans cosmid K06A...   290   e-103
AJ720214_1(AJ720214|pid:none) Gallus gallus mRNA for hypothetica...   283   e-103
protein update 2009. 4.24
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for AFK518 is mit
5' end seq. ID AFK518F
5' end seq. 
>AFK518F.Seq
AATATTTCTTTCTCTCTCTCAACACATTAAAATTTTTTTAAACACGTTAAATAAAAAAAA
TGATTAAAAAATTAATATTAGATCCAAAGAATGTATCAATAATTAAAAATGGTATTAGAA
ATTATTCAAAATCAAAATCATTTATTCAACCAATTACAACATTATCAGAAGAGGAAACTT
TATTAAAAGAAACTGTTGCAAATTTTGCAAATGAAAAAGTTAGACCATTAGTAAAGGTTA
TGGATGAAACATCAGAATTAAATAAAGGATTATTAAAAGATTTATTCGATATGAATTTAA
TGGGTATTGATATTTCAGATAGTTATGGTGGAGCCAATATGAATTTTATGGGATCAATCA
TTGCTATTGAAGAATTAGCAAAAGTTGATCCAGCCATTAGTGTAATAGTAGATGTACAAA
ATACACTCGTTAACAATTGTATCAATCGTTATGGTAGCATACAACAAAGAGAAAAGTATT
TATCAATGTTGGCAACCAACACAGTTGGTAGTTTTTGTCTAAGTGAATCAGGCCTCAGGT
TCTGATGCATTCGCATTGGCAACTCGTGCAGTCAGA----------
Length of 5' end seq. 576
3' end seq. ID AFK518Z
3' end seq. 
>AFK518Z.Seq
----------CGTGCAGTCAGACAATCAGATGGCACATTCGTTTTAAATGGCACTAACCA
ATGGATCACAAATGCCAAAGAGGCAGGTGTTTTCATCGTTATGGCAAATGTCGACCCATC
ACAGGGTTACAAAGGTATCACTGCCTTCATTGTTGAATCTAATAATCCAGGTTTAAGAAT
TGGTAAGAAGGAGGATAAATTAGGTATTAGAGCAAGCAGTACTTGTGAAGTTATTTTGGA
CAATTGTGTGGTAAAACCAACTGATATCCTCGGTGAATTGGGTAGAGGTTATAAAATTGC
TATCGAGGGTTTAAATGAAGGTCGTATTGGTATCGCTGCTCAAATGTTGGGTTTAGCTCA
AGGTGTATTCGATTCAACTATTCCTTACTTGATGGAACGTAAACAATTTGGTAAACCAAT
CGCTACTTTCCAAGGTATGCAATTCACTTATGCAGATTTAGCTGTAGATATTGAAGCTGG
TAGACTTTTAACTTATAATGCAGCTCGTATTAAAGAAGCTGGTCTACCATTTGTTTTCCA
AGCTTCAATGGCAAAACTTCATTGTTCACGTGTCGCTGAAAAAGCAGCTTCTGCTTGTAT
TTCAATGTTGGGTGGTGTTGGTTTCACTAAAGAATTCCCTGCTGAAAAATTCTTTAGAGA
TTCAAAAGTTGGTCAAATTTATGAAGGTACTTCAAATATTCAATTACAAACAATGCAAAA
GAAATGTTAAAAATTTAATAAATAAAATAAANAA
Length of 3' end seq. 744
Connected seq. ID AFK518P
Connected seq. 
>AFK518P.Seq
AATATTTCTTTCTCTCTCTCAACACATTAAAATTTTTTTAAACACGTTAAATAAAAAAAA
TGATTAAAAAATTAATATTAGATCCAAAGAATGTATCAATAATTAAAAATGGTATTAGAA
ATTATTCAAAATCAAAATCATTTATTCAACCAATTACAACATTATCAGAAGAGGAAACTT
TATTAAAAGAAACTGTTGCAAATTTTGCAAATGAAAAAGTTAGACCATTAGTAAAGGTTA
TGGATGAAACATCAGAATTAAATAAAGGATTATTAAAAGATTTATTCGATATGAATTTAA
TGGGTATTGATATTTCAGATAGTTATGGTGGAGCCAATATGAATTTTATGGGATCAATCA
TTGCTATTGAAGAATTAGCAAAAGTTGATCCAGCCATTAGTGTAATAGTAGATGTACAAA
ATACACTCGTTAACAATTGTATCAATCGTTATGGTAGCATACAACAAAGAGAAAAGTATT
TATCAATGTTGGCAACCAACACAGTTGGTAGTTTTTGTCTAAGTGAATCAGGCCTCAGGT
TCTGATGCATTCGCATTGGCAACTCGTGCAGTCAGA----------CGTGCAGTCAGACA
ATCAGATGGCACATTCGTTTTAAATGGCACTAACCAATGGATCACAAATGCCAAAGAGGC
AGGTGTTTTCATCGTTATGGCAAATGTCGACCCATCACAGGGTTACAAAGGTATCACTGC
CTTCATTGTTGAATCTAATAATCCAGGTTTAAGAATTGGTAAGAAGGAGGATAAATTAGG
TATTAGAGCAAGCAGTACTTGTGAAGTTATTTTGGACAATTGTGTGGTAAAACCAACTGA
TATCCTCGGTGAATTGGGTAGAGGTTATAAAATTGCTATCGAGGGTTTAAATGAAGGTCG
TATTGGTATCGCTGCTCAAATGTTGGGTTTAGCTCAAGGTGTATTCGATTCAACTATTCC
TTACTTGATGGAACGTAAACAATTTGGTAAACCAATCGCTACTTTCCAAGGTATGCAATT
CACTTATGCAGATTTAGCTGTAGATATTGAAGCTGGTAGACTTTTAACTTATAATGCAGC
TCGTATTAAAGAAGCTGGTCTACCATTTGTTTTCCAAGCTTCAATGGCAAAACTTCATTG
TTCACGTGTCGCTGAAAAAGCAGCTTCTGCTTGTATTTCAATGTTGGGTGGTGTTGGTTT
CACTAAAGAATTCCCTGCTGAAAAATTCTTTAGAGATTCAAAAGTTGGTCAAATTTATGA
AGGTACTTCAAATATTCAATTACAAACAATGCAAAAGAAATGTTAAAAATTTAATAAATA
AAATAAANAA
Length of connected seq. 1320
Full length Seq ID -
Full length Seq. -
Length of full length seq. -