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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLH514
Library SL
(Link to library)
Clone ID SLH514
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15327-1
Original site URL
Representative seq. ID SLH514E
(Link to Original site)
Representative DNA sequence 
>SLH514 (SLH514Q) /CSM/SL/SLH5-A/SLH514Q.Seq.d/
ATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATT
GGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATGCTATGAAACATAACTTATG
TAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTATGGAAGGTGTACCAGGTAAAAC
CAACAGTTTAAGAGTTCTTACCGTTGGTGAATCAATCAAAGATTTAAAGAAAAAATAAAA
ATATAGCCATAATTTTAATAAGCGCAGATTTAT
sequence update 1998. 7.13
Translated Amino Acid sequence 
YTVKHLLATRGAIPFLVESLVGTDKLVESCLEYAMKHNLCKKGSRVVIVSGVMEGVPGKT
NSLRVLTVGESIKDLKKK*kyshnfnkrrf
Translated Amino Acid sequence (All Frames) 
Frame A:
ihc*tftrh*rchsilgriigrh**tr*ilsricyet*lm*krfkschcfwcygrctr*n
qqfkssyrw*inqrfkekiki*p*f**aqiy


Frame B:
YTVKHLLATRGAIPFLVESLVGTDKLVESCLEYAMKHNLCKKGSRVVIVSGVMEGVPGKT
NSLRVLTVGESIKDLKKK*kyshnfnkrrf


Frame C:
tllniysplevpfhsw*nhwsalinslnlvsnml*nityvkkvqelslflvlwkvyqvkp
tv*eflplvnqski*rknkniaiilisadl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLH514 (SLH514Q) /CSM/SL/SLH5-A/SLH514Q.Seq.d/                        470   e-132
SLG254 (SLG254Q) /CSM/SL/SLG2-C/SLG254Q.Seq.d/                        470   e-132
SLE732 (SLE732Q) /CSM/SL/SLE7-B/SLE732Q.Seq.d/                        470   e-132
SFJ423 (SFJ423Q) /CSM/SF/SFJ4-A/SFJ423Q.Seq.d/                        460   e-129
SFC160 (SFC160Q) /CSM/SF/SFC1-C/SFC160Q.Seq.d/                        460   e-129
CFC605 (CFC605Q) /CSM/CF/CFC6-A/CFC605Q.Seq.d/                        460   e-129
SFJ336 (SFJ336Q) /CSM/SF/SFJ3-B/SFJ336Q.Seq.d/                        458   e-128
SFI138 (SFI138Q) /CSM/SF/SFI1-B/SFI138Q.Seq.d/                        458   e-128
SFB628 (SFB628Q) /CSM/SF/SFB6-B/SFB628Q.Seq.d/                        458   e-128
CFJ893 (CFJ893Q) /CSM/CF/CFJ8-D/CFJ893Q.Seq.d/                        458   e-128
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU039548) Dictyostelium discoideum slug cDNA, clone SLE732.      454   e-136  2
(AU039718) Dictyostelium discoideum slug cDNA, clone SLG254.      454   e-136  2
(AU039306) Dictyostelium discoideum slug cDNA, clone SLH514.      454   e-136  2
(BJ374238) Dictyostelium discoideum cDNA clone:ddc6i02, 3' e...   454   e-133  2
(BJ340018) Dictyostelium discoideum cDNA clone:dda11f12, 3' ...   454   e-133  2
(BJ339734) Dictyostelium discoideum cDNA clone:dda10o01, 3' ...   454   e-133  2
(BJ399349) Dictyostelium discoideum cDNA clone:dds5g15, 3' e...   454   e-133  2
(BJ340073) Dictyostelium discoideum cDNA clone:dda11d14, 3' ...   454   e-133  2
(BJ343201) Dictyostelium discoideum cDNA clone:dda22h09, 3' ...   454   e-133  2
(BJ343182) Dictyostelium discoideum cDNA clone:dda22d11, 3' ...   454   e-133  2
dna update 2009. 6.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RF5) RecName: Full=Pyruvate kinase;          Short=PK;      ...   151   8e-36
DQ466585_1(DQ466585|pid:none) Lactobacillus reuteri strain ATCC ...    52   5e-06
AP007281_722(AP007281|pid:none) Lactobacillus reuteri JCM 1112 D...    51   1e-05
AB050726_1(AB050726|pid:none) Toxoplasma gondii pk mRNA for pyru...    51   1e-05
AP008230_1608(AP008230|pid:none) Desulfitobacterium hafniense Y5...    51   1e-05
CP000716_901(CP000716|pid:none) Thermosipho melanesiensis BI429,...    50   2e-05
FM209186_3914(FM209186|pid:none) Pseudomonas aeruginosa LESB58 c...    49   7e-05
A97028(A97028) pyruvate kinase [imported] - Clostridium acetobut...    49   7e-05
CP000679_1751(CP000679|pid:none) Caldicellulosiruptor saccharoly...    49   7e-05
CP000724_3907(CP000724|pid:none) Alkaliphilus metalliredigens QY...    49   7e-05
protein update 2009. 7. 5
PSORT 

 psg: 0.82  gvh: 0.52  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SLH514 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID SLH514E
Full length Seq. 
>SLH514E.Seq
ATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATT
GGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATGCTATGAAACATAACTTATG
TAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTATGGAAGGTGTACCAGGTAAAAC
CAACAGTTTAAGAGTTCTTACCGTTGGTGAATCAATCAAAGATTTAAAGAAAAAATAAAA
ATATAGCCATAATTTTAATAAGCGCAGATTTAT
Length of full length seq. 273