>AFK480 (AFK480Q) /CSM/AF/AFK4-D/AFK480Q.Seq.d/
GAAAAACTAATAATATATTTAAGATGTCTGAAAATTCATCACCAAAATTAGAATCAACAT
CAGCAGCAGCAGCAAGTACAAAAAAACCATTCCCAGAATGGATTAAACCTAAAGGCAAAG
AAACTGAATTATTAATTAATAATAGTTTAACAGGTGGTAAAGTACCATTCGTTTTCAATG
AAAGTGGTAAAGGTAGATCATTAACATGGTATGCTTGTGGTCCAACAGTTTATGATGCAT
CACATATGGGTCATGCTAGAACTTATATTAGTTTTGATATCATTAGACGTATTATGAAAA
ATTATCTTGGTTTCAATATCCAATATGTTATGAACATTACCGATATTGATGATAAAATCA
TTATTCGTGCAAATGAAAATGGTATTTCACATAGTGATCTTTCAAAAAAATGGGAGACTG
CTTTCTTTGAAGATATGAAATTATTAAATGTATTACCACCAGATGCATTAACAAGAGTTA
CAGAATATGTACCACAAATTGTTGAATATGTTGAAAAGATTATTTCAAATGGATTTGCAT
ATGAATCAAATGGATCAGTTTATTTTGATACAGTAGCATTTTCAAAAGCACATGATTATG
GTAXXXXXXXXXX

KTNNIFKMSENSSPKLESTSAAAASTKKPFPEWIKPKGKETELLINNSLTGGKVPFVFNE
SGKGRSLTWYACGPTVYDASHMGHARTYISFDIIRRIMKNYLGFNIQYVMNITDIDDKII
IRANENGISHSDLSKKWETAFFEDMKLLNVLPPDALTRVTEYVPQIVEYVEKIISNGFAY
ESNGSVYFDTVAFSKAHDYG---

Frame A:
ekliiylrclkihhqn*nqhqqqqqvqknhsqnglnlkakklny*liiv*qvvkyhsfsm
kvvkvdh*hgmlvvqqfmmhhiwvmlelilvlisldvl*kiilvsisnml*tlpilmiks
lfvqmkmvfhivifqkngrllslki*ny*myyhqmh*qelqnmyhkllnmlkrlfqmdlh
mnqmdqfiliq*hfqkhmim---


Frame B:
kn**yi*dv*kfitkirinisssskykktiprmd*t*rqrn*iin***fnrw*stirfq*
kw*r*iinmvclwsnsl*citygsc*nly*f*yh*tyyeklswfqypicyehyry***nh
ysck*kwyft**sfkkmgdcfl*ryeiikcittrcinksyricttnc*ic*kdyfkwici
*ikwislf*yssifkst*lw---


Frame C:
KTNNIFKMSENSSPKLESTSAAAASTKKPFPEWIKPKGKETELLINNSLTGGKVPFVFNE
SGKGRSLTWYACGPTVYDASHMGHARTYISFDIIRRIMKNYLGFNIQYVMNITDIDDKII
IRANENGISHSDLSKKWETAFFEDMKLLNVLPPDALTRVTEYVPQIVEYVEKIISNGFAY
ESNGSVYFDTVAFSKAHDYG---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC506 (VHC506Q) /CSM/VH/VHC5-A/VHC506Q.Seq.d/                       1112   0.0
SFD396 (SFD396Q) /CSM/SF/SFD3-D/SFD396Q.Seq.d/                       1112   0.0
AFO629 (AFO629Q) /CSM/AF/AFO6-B/AFO629Q.Seq.d/                       1112   0.0
AFN666 (AFN666Q) /CSM/AF/AFN6-C/AFN666Q.Seq.d/                       1112   0.0
AFK480 (AFK480Q) /CSM/AF/AFK4-D/AFK480Q.Seq.d/                       1112   0.0
AFH281 (AFH281Q) /CSM/AF/AFH2-D/AFH281Q.Seq.d/                       1112   0.0
AFG246 (AFG246Q) /CSM/AF/AFG2-B/AFG246Q.Seq.d/                       1112   0.0
AFO752 (AFO752Q) /CSM/AF/AFO7-C/AFO752Q.Seq.d/                       1110   0.0
AFL214 (AFL214Q) /CSM/AF/AFL2-A/AFL214Q.Seq.d/                       1106   0.0
AFD870 (AFD870Q) /CSM/AF/AFD8-C/AFD870Q.Seq.d/                       1106   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014831|AE014831.1 Plasmodium falciparum 3D7 chromosome 10 section 3 of 7 of the complete sequence.    60   1e-11  11
CR382123|CR382123.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome C of strain NRRL  Y-1140 of Kluyveromyces lactis.    70   4e-08  17
AQ449880|AQ449880.1 500005E02.x1 CpIOWAM13mp18gDNA1 Cryptosporidium parvum genomic similar to cysteinyl-tRNA synthetase, DNA sequence.    66   7e-07  1
AB063521|AB063521.2 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome, section 1/2.    32   7e-06  15
AI980689|AI980689.1 pat.pk0031.a6.f chicken activated T cell cDNA Gallus gallus cDNA clone pat.pk0031.a6.f 5' similar to cysteinyl-tRNA synthetase, mRNA sequence.    42   2e-05  2
CO422681|CO422681.1 GGEZHT1014A04.g HT1 Gallus gallus cDNA clone GGEZHT1014A04, mRNA sequence.    42   3e-05  2
AJ454860|AJ454860.1 Gallus gallus EST, clone library riken1, clone 3o5r1.    42   1e-04  2
BX538350|BX538350.1 Cryptosporidium parvum chromosome 6, complete sequence; segment 1/4.    58   2e-04  1
CR382133|CR382133.1 Debaryomyces hansenii chromosome A of strain CBS767 of Debaryomyces hansenii.    34   4e-04  17
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    34   5e-04  14

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014185_147(AE014185|pid:none) Plasmodium falciparum 3D7 chromo...   191   1e-47
BX538350_42(BX538350|pid:none) Cryptosporidium parvum chromosome...   188   8e-47
AL590448_49(AL590448|pid:none) chromosome VIII of strain GB-M1 o...   172   6e-42
CR940352_62(CR940352|pid:none) Theileria annulata strain Ankara ...   163   3e-39
BC166536_1(BC166536|pid:none) Rattus norvegicus similar to hypot...   162   5e-39
(Q8BYM8) RecName: Full=Probable cysteinyl-tRNA synthetase, mitoc...   160   2e-38
AK021302_1(AK021302|pid:none) Mus musculus 13 days embryo stomac...   160   2e-38
AK009937_1(AK009937|pid:none) Mus musculus adult male tongue cDN...   160   2e-38
AK041962_1(AK041962|pid:none) Mus musculus 3 days neonate thymus...   160   2e-38
BC007220_1(BC007220|pid:none) Homo sapiens cysteinyl-tRNA synthe...   159   4e-38

 psg: 0.57  gvh: 0.38  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK480 is cyt

>AFK480F.Seq
GAAAAACTAATAATATATTTAAGATGTCTGAAAATTCATCACCAAAATTAGAATCAACAT
CAGCAGCAGCAGCAAGTACAAAAAAACCATTCCCAGAATGGATTAAACCTAAAGGCAAAG
AAACTGAATTATTAATTAATAATAGTTTAACAGGTGGTAAAGTACCATTCGTTTTCAATG
AAAGTGGTAAAGGTAGATCATTAACATGGTATGCTTGTGGTCCAACAGTTTATGATGCAT
CACATATGGGTCATGCTAGAACTTATATTAGTTTTGATATCATTAGACGTATTATGAAAA
ATTATCTTGGTTTCAATATCCAATATGTTATGAACATTACCGATATTGATGATAAAATCA
TTATTCGTGCAAATGAAAATGGTATTTCACATAGTGATCTTTCAAAAAAATGGGAGACTG
CTTTCTTTGAAGATATGAAATTATTAAATGTATTACCACCAGATGCATTAACAAGAGTTA
CAGAATATGTACCACAAATTGTTGAATATGTTGAAAAGATTATTTCAAATGGATTTGCAT
ATGAATCAAATGGATCAGTTTATTTTGATACAGTAGCATTTTCAAAAGCACATGATTATG
GTA----------