>SLH296 (SLH296Q) /CSM/SL/SLH2-D/SLH296Q.Seq.d/
GGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGG
TTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACA
CACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATC
AAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGA
TGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGA
ACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGAC
CCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTT
ATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTC
CCACACTGTTCCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTX
XXXXXXXXX

DGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLD-
--

Frame A:
gr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcygrygskrllrr**spi
kerylnpqipn*trycyqlg*ygknlasyflq*tpccsrrtpssln*spikskgqqrknd
pnyv*nlqhpshvrchsscliliclwsyhrycygfr*wclphcsnl*rlclttchltfr-
--


Frame B:
DGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLD-
--


Frame C:
tvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkktlt*vmkpnq
revs*psntqlntvllptgmiwkksgiilstmnsvllqkntqfs*lkph*iqrptekk*p
klclkpstpqpctlpfklsypymplvvppvllwiqvmvsptlfqsmkvmpyhmpsyv*--
-

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                       1068   0.0
VFM725 (VFM725Q) /CSM/VF/VFM7-B/VFM725Q.Seq.d/                       1068   0.0
VFM720 (VFM720Q) /CSM/VF/VFM7-A/VFM720Q.Seq.d/                       1068   0.0
VFM705 (VFM705Q) /CSM/VF/VFM7-A/VFM705Q.Seq.d/                       1068   0.0
VFM609 (VFM609Q) /CSM/VF/VFM6-A/VFM609Q.Seq.d/                       1068   0.0
VFM283 (VFM283Q) /CSM/VF/VFM2-D/VFM283Q.Seq.d/                       1068   0.0
VFK573 (VFK573Q) /CSM/VF/VFK5-D/VFK573Q.Seq.d/                       1068   0.0
VFJ616 (VFJ616Q) /CSM/VF/VFJ6-A/VFJ616Q.Seq.d/                       1068   0.0
VFJ346 (VFJ346Q) /CSM/VF/VFJ3-B/VFJ346Q.Seq.d/                       1068   0.0
VFI269 (VFI269Q) /CSM/VF/VFI2-C/VFI269Q.Seq.d/                       1068   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...  1068   0.0    1
(AU284440) Dictyostelium discoideum gamete cDNA clone:FC-AV1...  1068   0.0    1
(AU062036) Dictyostelium discoideum slug cDNA, clone SLH296.     1068   0.0    1
(AU061021) Dictyostelium discoideum slug cDNA, clone SLC645.     1068   0.0    1
(AU060166) Dictyostelium discoideum slug cDNA, clone SLA505.     1068   0.0    1
(BJ416678) Dictyostelium discoideum cDNA clone:ddv26n02, 5' ...  1068   0.0    1
(BJ416659) Dictyostelium discoideum cDNA clone:ddv26j01, 5' ...  1068   0.0    1
(BJ416651) Dictyostelium discoideum cDNA clone:ddv26h05, 5' ...  1068   0.0    1
(BJ416616) Dictyostelium discoideum cDNA clone:ddv26a04, 5' ...  1068   0.0    1
(BJ416521) Dictyostelium discoideum cDNA clone:ddv26n16, 5' ...  1068   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   369   e-101
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   369   e-101
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   369   e-101
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   367   e-101
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   363   2e-99
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        363   2e-99
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   362   4e-99
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   361   6e-99
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   360   1e-98
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   360   2e-98

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.07  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for SLH296 is nuc

>SLH296F.Seq
GGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGG
TTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACA
CACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATC
AAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGA
TGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGA
ACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGAC
CCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTT
ATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTC
CCACACTGTTCCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATT-
---------