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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLH284
Library SL
(Link to library)
Clone ID SLH284
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15748-1
Original site URL
Representative seq. ID SLH284P
(Link to Original site)
Representative DNA sequence 
>SLH284 (SLH284Q) /CSM/SL/SLH2-D/SLH284Q.Seq.d/
CCTATTATGAAAATTGTCAATCGAGCTTCTTCAACCAGATTGTATGGATTCAGTGGGAGC
ACCAGTTCAACCAAGAGTGGTCTTGGGTGGTTGGGAGAATAATAATTCCTCTGCTGCATC
TGCTTTTGTTGTTACCTTCCTTTTAAATAATCCAACCGATATGGTAAATACCGCCATGGC
ATGGGAACAAGTTTGGTTGGATCATATCTCTGCCATTGCTGCCACTTCTGAAATTTTCGA
TATCATATATAGTGCCGAGCGTTCAGTTCAAGATGAGTTATCACGTGAAGGTAATGCCGA
TATTCCAACTATTCTAATCTCTTATTTCGTTATGTTCTTCTATGTCTCCCTTTCATTGGG
TAGTTACTATCCATTCCCAACTCGTTTCCTCTCATTATTTGTCCGTTCGCGTTTTGCACT
TGGTTTTTGTGGTATCATCATTGTAGCTGCCTCCATCGTCATTAGTGTTGGTGTTTGTAG
TATGGCAAATTTAAAGGCAACCTTAATCATTTCCGAAGTAATTCCATTCCTTGGTATTAG
CAATXXXXXXXXXXTCTTGGTTGTAATTTCCGTTGGTATGATTTGTGTCGATTTATTAGG
TATCATGGCATTATGGAACATCTCTTTGAATGCAGTTTCCGTTGTAAACGTTGTTATGGC
CATTGGTATTGGTATCGAGTTTTGTGTTCACATTGCTTCAACCTTTATCAATGCTCCAAA
ACATTTCTCAAGAGATCAAAAAGCAAAATATGCCGTCACTGAGATGGGTAGTTCCATCAT
TAGTGGTATCTTTATCACAAAATTATTGGGTGTCGTGGTTTTAGGTTTCTCAACTTCAGA
AATTTTCACTGTTTATTACTTTAGAATGTATCTCAGTATAGTTTTCTTGGGTGGTTTACA
TGGTTTAGTTTTCTTACCAGTATTATTATCTTTATTTGGAACTGATACCCTTCAATTAGA
TCGTCTTTGTAAATCTAAAAAAAATAGTTATTCACTTAATGATGAATAAAAAAAAAAAAG
T
sequence update 1999. 2. 9
Translated Amino Acid sequence 
ll*KLSIELLQPDCMDSVGAPVQPRVVLGGWENNNSSAASAFVVTFLLNNPTDMVNTAMA
WEQVWLDHISAIAATSEIFDIIYSAERSVQDELSREGNADIPTILISYFVMFFYVSLSLG
SYYPFPTRFLSLFVRSRFALGFCGIIIVAASIVISVGVCSMANLKATLIISEVIPFLGIS
N---

---LVVISVGMICVDLLGIMALWNISLNAVSVVNVVMAIGIGIEFCVHIASTFINAPKHF
SRDQKAKYAVTEMGSSIISGIFITKLLGVVVLGFSTSEIFTVYYFRMYLSIVFLGGLHGL
VFLPVLLSLFGTDTLQLDRLCKSKKNSYSLNDE*kkks
Translated Amino Acid sequence (All Frames) 
Frame A:
pimkivnrasstrlygfsgstsstksglgwlge**flccicfccylpfk*snrygkyrhg
mgtslvgsylchcchf*nfryhi*crafssr*vit*r*crysnysnllfryvllclpfig
*llsipnsfpliicpfafctwflwyhhcsclhrh*cwcl*ygkfkgnlnhfrsnsipwy*
q---

---swl*fplv*fvsiy*vswhygtsl*mqfpl*tllwplvlvssfvftllqplsmlqni
sqeikkqnmpslrwvvpslvvslsqnywvswf*vsqlqkfslfitlecisv*fswvvymv
*fsyqyyylylelipfn*ivfvnlkkivihlmmnkkkk

Frame B:
ll*KLSIELLQPDCMDSVGAPVQPRVVLGGWENNNSSAASAFVVTFLLNNPTDMVNTAMA
WEQVWLDHISAIAATSEIFDIIYSAERSVQDELSREGNADIPTILISYFVMFFYVSLSLG
SYYPFPTRFLSLFVRSRFALGFCGIIIVAASIVISVGVCSMANLKATLIISEVIPFLGIS
N---

---lgcnfrwydlcrfiryhgimehlfecsfrckrcyghwywyrvlcshcfnlyqcsktf
lkrskskicrh*dg*fhh*wylyhkiigcrgfrflnfrnfhclll*nvsqysflgwftwf
sfltsiiifiwn*ypsirssl*i*kk*lft***ikkkk

Frame C:
yyencqssffnqivwiqwehqfnqewswvvgriiipllhlllllpsf*iiqpiw*ippwh
gnkfgwiislpllpllkfsisyivpsvqfkmsyhvkvmpifqlf*slislcssmspfhwv
vtihsqlvsshylsvrvlhlvfvvssl*lppsslvlvfvvwqi*rqp*sfpk*fhslvla
---

---LVVISVGMICVDLLGIMALWNISLNAVSVVNVVMAIGIGIEFCVHIASTFINAPKHF
SRDQKAKYAVTEMGSSIISGIFITKLLGVVVLGFSTSEIFTVYYFRMYLSIVFLGGLHGL
VFLPVLLSLFGTDTLQLDRLCKSKKNSYSLNDE*kkks
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLH284 (SLH284Q) /CSM/SL/SLH2-D/SLH284Q.Seq.d/                       1889   0.0
AHI859 (AHI859Q) /CSM/AH/AHI8-C/AHI859Q.Seq.d/                       1041   0.0
AHG830 (AHG830Q) /CSM/AH/AHG8-B/AHG830Q.Seq.d/                        896   0.0
AHN351 (AHN351Q) /CSM/AH/AHN3-C/AHN351Q.Seq.d/                        864   0.0
VHQ103 (VHQ103Q) /CSM/VH/VHQ1-A/VHQ103Q.Seq.d/                        837   0.0
VHI844 (VHI844Q) /CSM/VH/VHI8-B/VHI844Q.Seq.d/                        837   0.0
SHJ888 (SHJ888Q) /CSM/SH/SHJ8-D/SHJ888Q.Seq.d/                        837   0.0
SHG768 (SHG768Q) /CSM/SH/SHG7-C/SHG768Q.Seq.d/                        837   0.0
SHG328 (SHG328Q) /CSM/SH/SHG3-B/SHG328Q.Seq.d/                        837   0.0
SHD228 (SHD228Q) /CSM/SH/SHD2-B/SHD228Q.Seq.d/                        837   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU062031) Dictyostelium discoideum slug cDNA, clone SLH284.     1078   0.0    1
(AF202726) Dictyostelium discoideum NPC1 protein gene, compl...   993   0.0    3
(AF202725) Dictyostelium discoideum NPC1 protein mRNA, compl...   993   0.0    3
(BJ334999) Dictyostelium discoideum cDNA clone:dda48f16, 5' ...   993   0.0    2
(AU039252) Dictyostelium discoideum slug cDNA, clone SLH284.      898   0.0    1
(BJ333890) Dictyostelium discoideum cDNA clone:dda44l08, 5' ...   854   0.0    2
(BJ442077) Dictyostelium discoideum cDNA clone:ddv48d20, 3' ...   882   0.0    1
(BJ407141) Dictyostelium discoideum cDNA clone:dds37h17, 3' ...   882   0.0    1
(BJ405007) Dictyostelium discoideum cDNA clone:dds30g08, 3' ...   882   0.0    1
(BJ380014) Dictyostelium discoideum cDNA clone:ddc36p18, 3' ...   882   0.0    1
dna update 2009. 5.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF202725_1(AF202725|pid:none) Dictyostelium discoideum NPC1 prot...   301   1e-80
BT009977_1(BT009977|pid:none) Drosophila melanogaster RE56428 fu...   116   1e-24
AE014134_1855(AE014134|pid:none) Drosophila melanogaster chromos...   116   1e-24
AY667365_1(AY667365|pid:none) Ciona intestinalis isolate west co...   115   2e-24
AY667352_1(AY667352|pid:none) Ciona intestinalis isolate west co...   115   2e-24
AF503634_1(AF503634|pid:none) Felis catus Niemann-Pick C1 mRNA, ...   115   2e-24
AF258783_1(AF258783|pid:none) Felis catus Niemann-Pick type C1 d...   115   2e-24
AY667348_1(AY667348|pid:none) Ciona intestinalis isolate west co...   115   2e-24
AF182744_1(AF182744|pid:none) Cricetulus griseus Niemann-Pick ty...   115   3e-24
AC025815_1(AC025815|pid:none) Arabidopsis thaliana chromosome 1 ...   114   4e-24
protein update 2009. 6. 4
PSORT 

 psg: 0.75  gvh: 0.38  alm: 0.17  top: 0.93  tms: 0.47  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 56.0 %: plasma membrane
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for SLH284 is pla
5' end seq. ID SLH284F
5' end seq. 
>SLH284F.Seq
CCTATTATGAAAATTGTCAATCGAGCTTCTTCAACCAGATTGTATGGATTCAGTGGGAGC
ACCAGTTCAACCAAGAGTGGTCTTGGGTGGTTGGGAGAATAATAATTCCTCTGCTGCATC
TGCTTTTGTTGTTACCTTCCTTTTAAATAATCCAACCGATATGGTAAATACCGCCATGGC
ATGGGAACAAGTTTGGTTGGATCATATCTCTGCCATTGCTGCCACTTCTGAAATTTTCGA
TATCATATATAGTGCCGAGCGTTCAGTTCAAGATGAGTTATCACGTGAAGGTAATGCCGA
TATTCCAACTATTCTAATCTCTTATTTCGTTATGTTCTTCTATGTCTCCCTTTCATTGGG
TAGTTACTATCCATTCCCAACTCGTTTCCTCTCATTATTTGTCCGTTCGCGTTTTGCACT
TGGTTTTTGTGGTATCATCATTGTAGCTGCCTCCATCGTCATTAGTGTTGGTGTTTGTAG
TATGGCAAATTTAAAGGCAACCTTAATCATTTCCGAAGTAATTCCATTCCTTGGTATTAG
CAAT----------
Length of 5' end seq. 544
3' end seq. ID SLH284Z
3' end seq. 
>SLH284Z.Seq
----------TCTTGGTTGTAATTTCCGTTGGTATGATTTGTGTCGATTTATTAGGTATC
ATGGCATTATGGAACATCTCTTTGAATGCAGTTTCCGTTGTAAACGTTGTTATGGCCATT
GGTATTGGTATCGAGTTTTGTGTTCACATTGCTTCAACCTTTATCAATGCTCCAAAACAT
TTCTCAAGAGATCAAAAAGCAAAATATGCCGTCACTGAGATGGGTAGTTCCATCATTAGT
GGTATCTTTATCACAAAATTATTGGGTGTCGTGGTTTTAGGTTTCTCAACTTCAGAAATT
TTCACTGTTTATTACTTTAGAATGTATCTCAGTATAGTTTTCTTGGGTGGTTTACATGGT
TTAGTTTTCTTACCAGTATTATTATCTTTATTTGGAACTGATACCCTTCAATTAGATCGT
CTTTGTAAATCTAAAAAAAATAGTTATTCACTTAATGATGAATAAAAAAAAAAAAGT
Length of 3' end seq. 467
Connected seq. ID SLH284P
Connected seq. 
>SLH284P.Seq
CCTATTATGAAAATTGTCAATCGAGCTTCTTCAACCAGATTGTATGGATTCAGTGGGAGC
ACCAGTTCAACCAAGAGTGGTCTTGGGTGGTTGGGAGAATAATAATTCCTCTGCTGCATC
TGCTTTTGTTGTTACCTTCCTTTTAAATAATCCAACCGATATGGTAAATACCGCCATGGC
ATGGGAACAAGTTTGGTTGGATCATATCTCTGCCATTGCTGCCACTTCTGAAATTTTCGA
TATCATATATAGTGCCGAGCGTTCAGTTCAAGATGAGTTATCACGTGAAGGTAATGCCGA
TATTCCAACTATTCTAATCTCTTATTTCGTTATGTTCTTCTATGTCTCCCTTTCATTGGG
TAGTTACTATCCATTCCCAACTCGTTTCCTCTCATTATTTGTCCGTTCGCGTTTTGCACT
TGGTTTTTGTGGTATCATCATTGTAGCTGCCTCCATCGTCATTAGTGTTGGTGTTTGTAG
TATGGCAAATTTAAAGGCAACCTTAATCATTTCCGAAGTAATTCCATTCCTTGGTATTAG
CAAT----------TCTTGGTTGTAATTTCCGTTGGTATGATTTGTGTCGATTTATTAGG
TATCATGGCATTATGGAACATCTCTTTGAATGCAGTTTCCGTTGTAAACGTTGTTATGGC
CATTGGTATTGGTATCGAGTTTTGTGTTCACATTGCTTCAACCTTTATCAATGCTCCAAA
ACATTTCTCAAGAGATCAAAAAGCAAAATATGCCGTCACTGAGATGGGTAGTTCCATCAT
TAGTGGTATCTTTATCACAAAATTATTGGGTGTCGTGGTTTTAGGTTTCTCAACTTCAGA
AATTTTCACTGTTTATTACTTTAGAATGTATCTCAGTATAGTTTTCTTGGGTGGTTTACA
TGGTTTAGTTTTCTTACCAGTATTATTATCTTTATTTGGAACTGATACCCTTCAATTAGA
TCGTCTTTGTAAATCTAAAAAAAATAGTTATTCACTTAATGATGAATAAAAAAAAAAAAG
T
Length of connected seq. 1011
Full length Seq ID -
Full length Seq. -
Length of full length seq. -