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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLH282
Library SL
(Link to library)
Clone ID SLH282
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10985-1|Contig-U15908-1
Original site URL
Representative seq. ID SLH282P
(Link to Original site)
Representative DNA sequence 
>SLH282 (SLH282Q) /CSM/SL/SLH2-D/SLH282Q.Seq.d/
GGTTTTTAAAACTATTGATACTGAAGAGAATGGAATTATATCAATAACACAATTAAGACA
AGAGATTGAATCATTAACTGGTGAATCAAGTGAAACCAATCCATTAATTGGTAAATTATT
AAATTTTTTAAGAATTGTTCATAGAGCTGAACAACCAAAAAATGATGATGACGAAGACGA
AAATGAAGACGAACAACAAGAGGGTGATGATCAAGAAAATACAGGTGGTAGTGATTTTGA
AGATGATTCATCATTTGAAGATTTAATATTAAATGAAAAGAAACAAATTGAAGAAGATAA
TACATTTGTTGATCTTCAATCATTTTTAGAATCTATGGCAAGTTATATGGATGGTGAAGA
TCCAAAAAAATTACGTGAGAAACAACGTGAACAACGTCGTCTTGAGAAAGAACAACGTGA
AAAGGAGGATAAAGAGCGTGAAAAGGAATTAAAACGTCAAAAAGAGGAACGTGAACGTGA
AAAGGAACAACGTCGTCTCGAAAAGGXXXXXXXXXXAGAACAAATTGAGAGAGAGGTCGA
AGAGGAAAGCAAAAGAATTGAAGCTGACCGTGAAGCTAGAAAGAAAGCCAGAGAACTCGA
TAGACCATCAGTATCCTCTCCAATCTCTTCCTCTTCCTCACCTTCAAATCCTTCCTCCTC
ATCACCAACTCCAATCAGTAATACCTCTCCTTCAAATTCAAGTGGTAATCTCATGCTCTC
TGAACAAGAACAACGTAGAAGAGATCGTGAACAACGTCGTTTAGAATTAGAACAAGAAGC
TAATAAACTTGAAGAAGAACGTAAGAAAAGACAAGAAGAAAGAAGACTCGCAAGAGAACA
AAAAGAAAGAGAATTGGAAGAAGAAACAAAAAGACTTGAACAAGAAAGAGAAGAACGTCG
TAGAAAGAGAGAAGAAGCTAAAAATTCTTAAATCTCATTTCTTATTAATTTTTAAAAAAA
AAAAAAAATCC
sequence update 1999. 2. 9
Translated Amino Acid sequence 
VFKTIDTEENGIISITQLRQEIESLTGESSETNPLIGKLLNFLRIVHRAEQPKNDDDEDE
NEDEQQEGDDQENTGGSDFEDDSSFEDLILNEKKQIEEDNTFVDLQSFLESMASYMDGED
PKKLREKQREQRRLEKEQREKEDKEREKELKRQKEEREREKEQRRLEK---

---EQIEREVEEESKRIEADREARKKARELDRPSVSSPISSSSSPSNPSSSSPTPISNTS
PSNSSGNLMLSEQEQRRRDREQRRLELEQEANKLEEERKKRQEERRLAREQKERELEEET
KRLEQEREERRRKREEAKNS*isflinf*kkkki
Translated Amino Acid sequence (All Frames) 
Frame A:
gf*ny*y*rewnyinntiktrd*iinw*ik*nqsinw*iikffkncs*s*ttkk***rrr
k*rrttrg**srkyrw**f*r*fii*rfnik*ketn*rr*yic*ssiifriygklygw*r
skkit*ett*ttss*ertt*kgg*ra*kgiktskrgt*t*kgttssrk---

---rtn*ergrrgkqkn*s*p*s*kesqrtr*tisilsnlflfltfksfllitnsnq*yl
sfkfkw*shal*trtt*krs*ttsfrirtrs**t*rrt*ektrrkktrkrtkrkrigrrn
kkt*trkrrts*kerrs*kflnlisy*flkkkkn

Frame B:
VFKTIDTEENGIISITQLRQEIESLTGESSETNPLIGKLLNFLRIVHRAEQPKNDDDEDE
NEDEQQEGDDQENTGGSDFEDDSSFEDLILNEKKQIEEDNTFVDLQSFLESMASYMDGED
PKKLREKQREQRRLEKEQREKEDKEREKELKRQKEEREREKEQRRLEK---

---EQIEREVEEESKRIEADREARKKARELDRPSVSSPISSSSSPSNPSSSSPTPISNTS
PSNSSGNLMLSEQEQRRRDREQRRLELEQEANKLEEERKKRQEERRLAREQKERELEEET
KRLEQEREERRRKREEAKNS*isflinf*kkkki

Frame C:
flkllilkrmelyq*hn*dkrlnh*lvnqvkpih*lvny*if*elfielnnqkmmmtktk
mktnnkrvmikkiqvvvilkmihhlki*y*mkrnklkkiihllifnhf*nlwqviwmvki
qknyvrnnvnnvvlrknnvkrriksvkrn*nvkkrnvnvkrnnvvskr---

---nklrerskrkakelkltvklerkpensidhqyplqslplphlqilpphhqlqsvipl
lqiqvviscslnknnveeivnnvv*n*nkklinlkknvrkdkkkedsqenkkkenwkkkq
kdlnkkeknvverekklkilkshfllifkkkkks
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLH282 (SLH282Q) /CSM/SL/SLH2-D/SLH282Q.Seq.d/                       1463   0.0
VFM782 (VFM782Q) /CSM/VF/VFM7-D/VFM782Q.Seq.d/                        722   0.0
SLG793 (SLG793Q) /CSM/SL/SLG7-D/SLG793Q.Seq.d/                        533   e-150
SLE688 (SLE688Q) /CSM/SL/SLE6-D/SLE688Q.Seq.d/                        533   e-150
AFJ534 (AFJ534Q) /CSM/AF/AFJ5-B/AFJ534Q.Seq.d/                        519   e-146
SLK426 (SLK426Q) /CSM/SL/SLK4-B/SLK426Q.Seq.d/                        494   e-138
CHK558 (CHK558Q) /CSM/CH/CHK5-C/CHK558Q.Seq.d/                        337   2e-91
SLI189 (SLI189Q) /CSM/SL/SLI1-D/SLI189Q.Seq.d/                        266   6e-70
SLE655 (SLE655Q) /CSM/SL/SLE6-C/SLE655Q.Seq.d/                        266   6e-70
AFB739 (AFB739Q) /CSM/AF/AFB7-B/AFB739Q.Seq.d/                        258   1e-67
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU062030) Dictyostelium discoideum slug cDNA, clone SLH282.      720   0.0    2
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...   720   0.0    2
(BJ416551) Dictyostelium discoideum cDNA clone:ddv26d21, 5' ...   720   0.0    2
(AU034917) Dictyostelium discoideum slug cDNA, clone SLE688.      182   e-99   5
(AU039941) Dictyostelium discoideum slug cDNA, clone SLG793.      182   1e-98  4
(AU039251) Dictyostelium discoideum slug cDNA, clone SLH282.      190   4e-97  3
(BJ344713) Dictyostelium discoideum cDNA clone:dda20c09, 3' ...   182   3e-91  4
(BJ367653) Dictyostelium discoideum cDNA clone:ddc43c15, 5' ...   335   3e-90  2
(AU054017) Dictyostelium discoideum slug cDNA, clone SLK426.      168   5e-83  3
(AU053233) Dictyostelium discoideum slug cDNA, clone SLI189.      262   1e-66  2
dna update 2009. 5.19
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3159261
Number of Hits to DB: 664,656,050
protein update 2009. 6. 4
PSORT 

 psg: 0.75  gvh: 0.45  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.36  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SLH282 is nuc
5' end seq. ID SLH282F
5' end seq. 
>SLH282F.Seq
GGTTTTTAAAACTATTGATACTGAAGAGAATGGAATTATATCAATAACACAATTAAGACA
AGAGATTGAATCATTAACTGGTGAATCAAGTGAAACCAATCCATTAATTGGTAAATTATT
AAATTTTTTAAGAATTGTTCATAGAGCTGAACAACCAAAAAATGATGATGACGAAGACGA
AAATGAAGACGAACAACAAGAGGGTGATGATCAAGAAAATACAGGTGGTAGTGATTTTGA
AGATGATTCATCATTTGAAGATTTAATATTAAATGAAAAGAAACAAATTGAAGAAGATAA
TACATTTGTTGATCTTCAATCATTTTTAGAATCTATGGCAAGTTATATGGATGGTGAAGA
TCCAAAAAAATTACGTGAGAAACAACGTGAACAACGTCGTCTTGAGAAAGAACAACGTGA
AAAGGAGGATAAAGAGCGTGAAAAGGAATTAAAACGTCAAAAAGAGGAACGTGAACGTGA
AAAGGAACAACGTCGTCTCGAAAAGG----------
Length of 5' end seq. 506
3' end seq. ID SLH282Z
3' end seq. 
>SLH282Z.Seq
----------AGAACAAATTGAGAGAGAGGTCGAAGAGGAAAGCAAAAGAATTGAAGCTG
ACCGTGAAGCTAGAAAGAAAGCCAGAGAACTCGATAGACCATCAGTATCCTCTCCAATCT
CTTCCTCTTCCTCACCTTCAAATCCTTCCTCCTCATCACCAACTCCAATCAGTAATACCT
CTCCTTCAAATTCAAGTGGTAATCTCATGCTCTCTGAACAAGAACAACGTAGAAGAGATC
GTGAACAACGTCGTTTAGAATTAGAACAAGAAGCTAATAAACTTGAAGAAGAACGTAAGA
AAAGACAAGAAGAAAGAAGACTCGCAAGAGAACAAAAAGAAAGAGAATTGGAAGAAGAAA
CAAAAAGACTTGAACAAGAAAGAGAAGAACGTCGTAGAAAGAGAGAAGAAGCTAAAAATT
CTTAAATCTCATTTCTTATTAATTTTTAAAAAAAAAAAAAAATCC
Length of 3' end seq. 455
Connected seq. ID SLH282P
Connected seq. 
>SLH282P.Seq
GGTTTTTAAAACTATTGATACTGAAGAGAATGGAATTATATCAATAACACAATTAAGACA
AGAGATTGAATCATTAACTGGTGAATCAAGTGAAACCAATCCATTAATTGGTAAATTATT
AAATTTTTTAAGAATTGTTCATAGAGCTGAACAACCAAAAAATGATGATGACGAAGACGA
AAATGAAGACGAACAACAAGAGGGTGATGATCAAGAAAATACAGGTGGTAGTGATTTTGA
AGATGATTCATCATTTGAAGATTTAATATTAAATGAAAAGAAACAAATTGAAGAAGATAA
TACATTTGTTGATCTTCAATCATTTTTAGAATCTATGGCAAGTTATATGGATGGTGAAGA
TCCAAAAAAATTACGTGAGAAACAACGTGAACAACGTCGTCTTGAGAAAGAACAACGTGA
AAAGGAGGATAAAGAGCGTGAAAAGGAATTAAAACGTCAAAAAGAGGAACGTGAACGTGA
AAAGGAACAACGTCGTCTCGAAAAGG----------AGAACAAATTGAGAGAGAGGTCGA
AGAGGAAAGCAAAAGAATTGAAGCTGACCGTGAAGCTAGAAAGAAAGCCAGAGAACTCGA
TAGACCATCAGTATCCTCTCCAATCTCTTCCTCTTCCTCACCTTCAAATCCTTCCTCCTC
ATCACCAACTCCAATCAGTAATACCTCTCCTTCAAATTCAAGTGGTAATCTCATGCTCTC
TGAACAAGAACAACGTAGAAGAGATCGTGAACAACGTCGTTTAGAATTAGAACAAGAAGC
TAATAAACTTGAAGAAGAACGTAAGAAAAGACAAGAAGAAAGAAGACTCGCAAGAGAACA
AAAAGAAAGAGAATTGGAAGAAGAAACAAAAAGACTTGAACAAGAAAGAGAAGAACGTCG
TAGAAAGAGAGAAGAAGCTAAAAATTCTTAAATCTCATTTCTTATTAATTTTTAAAAAAA
AAAAAAAATCC
Length of connected seq. 961
Full length Seq ID -
Full length Seq. -
Length of full length seq. -