>AFK470 (AFK470Q) /CSM/AF/AFK4-C/AFK470Q.Seq.d/
CCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTTATGCCCCATGGTGCGN
NCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAACAATTATCAGCCAACAA
AAAGATTGCAATTGCAAAAGTTGATTGTACTCANCANGAACAACTCTGTTTNCAAAATAA
AGTTCAAGGTTATCCAACTTTAGTTGTNGNNGGGAATGGNAAAGCTGAACCATATGAAGG
NGATAGAACCACTAAATCAATNGNTCAAACTTTAGAANAAGAXXXXXXXXXXGATGTAAA
GAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATT
CCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGA
TTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATAC
CGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCA
ACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAA
GTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCA
TTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATC
TGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAG
AGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGA
AGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATNAA
TTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCGCTAA

HNSVSEHDVTLVMFYAPWCXHCKTLKPLYEEAAKQLSANKKIAIAKVDCTXXEQLCXQNK
VQGYPTLVVXGNGKAEPYEGDRTTKSXXQTLEX---

---DVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKE
TFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFY
APWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKE
NPISYEGQRNDHMNFVEFIQDNAAXNSNYHHLKLMIMXI*krffa

Frame A:
p*fsf*t*rnscyvlcpmvrxl*nfktii*rsc*tiisqqkdcncks*lysxxttlfxk*
ssrlsnfscxxewxs*ti*rx*nh*inxsnfrxr---

---DVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKE
TFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFY
APWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKE
NPISYEGQRNDHMNFVEFIQDNAAXNSNYHHLKLMIMXI*krffa

Frame B:
HNSVSEHDVTLVMFYAPWCXHCKTLKPLYEEAAKQLSANKKIAIAKVDCTXXEQLCXQNK
VQGYPTLVVXGNGKAEPYEGDRTTKSXXQTLEX---

---m*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkk
pshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnst
lhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkk
iqslmkvkemii*tllnsfkitlpxiqitiisn***cxskkdssl

Frame C:
iiqflnmt*lllcfmphgaxivkl*nhymkkllnnyqptkrlqlqklivlxxnnsvxkik
fkviql*lxxgmxklnhmkxieplnqxxkl*xk---

---ckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krn
lliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilr
smvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrk
snll*rskk*syelc*ihsr*rchxfklpssqtddnvnlkkilr*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK470 (AFK470Q) /CSM/AF/AFK4-C/AFK470Q.Seq.d/                       1754   0.0
AFJ889 (AFJ889Q) /CSM/AF/AFJ8-D/AFJ889Q.Seq.d/                       1312   0.0
AFO363 (AFO363Q) /CSM/AF/AFO3-C/AFO363Q.Seq.d/                       1302   0.0
AFM548 (AFM548Q) /CSM/AF/AFM5-B/AFM548Q.Seq.d/                       1302   0.0
AFM212 (AFM212Q) /CSM/AF/AFM2-A/AFM212Q.Seq.d/                       1302   0.0
AFK779 (AFK779Q) /CSM/AF/AFK7-D/AFK779Q.Seq.d/                       1302   0.0
AFK539 (AFK539Q) /CSM/AF/AFK5-B/AFK539Q.Seq.d/                       1302   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1302   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1302   0.0
AFJ720 (AFJ720Q) /CSM/AF/AFJ7-A/AFJ720Q.Seq.d/                       1302   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   3e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   5e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   5e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   5e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   5e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   7e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   7e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   7e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   7e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U41542_7(U41542|pid:none) Caenorhabditis elegans cosmid C07A12, ...   177   4e-43
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   172   2e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   5e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   167   6e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   2e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   160   4e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   6e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   3e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   4e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   4e-35

 psg: 0.75  gvh: 0.46  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFK470 is cyt

>AFK470F.Seq
CCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTTATGCCCCATGGTGCGN
NCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAACAATTATCAGCCAACAA
AAAGATTGCAATTGCAAAAGTTGATTGTACTCANCANGAACAACTCTGTTTNCAAAATAA
AGTTCAAGGTTATCCAACTTTAGTTGTNGNNGGGAATGGNAAAGCTGAACCATATGAAGG
NGATAGAACCACTAAATCAATNGNTCAAACTTTAGAANAAGA----------

>AFK470Z.Seq
----------GATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTG
GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGT
TCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGA
AACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTC
TCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGT
TGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTA
CGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATA
CTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACC
AAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGA
AAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCA
AGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTA
AAAAAGATTCTTCGCTAA

>AFK470P.Seq
CCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTTATGCCCCATGGTGCGN
NCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAACAATTATCAGCCAACAA
AAAGATTGCAATTGCAAAAGTTGATTGTACTCANCANGAACAACTCTGTTTNCAAAATAA
AGTTCAAGGTTATCCAACTTTAGTTGTNGNNGGGAATGGNAAAGCTGAACCATATGAAGG
NGATAGAACCACTAAATCAATNGNTCAAACTTTAGAANAAGA----------GATGTAAA
GAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATT
CCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGA
TTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATAC
CGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCA
ACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAA
GTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCA
TTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATC
TGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAG
AGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGA
AGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATNAA
TTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCGCTAA