>AFK467 (AFK467Q) /CSM/AF/AFK4-C/AFK467Q.Seq.d/
XXXXXXXXXXCCACTCAAGATAACANTCAAGTTNTNGNAANATGTAAAGAAAATNGNTNC
CTCNCAAAAAGGTAATGCCGTTTTNTGTTGGGTCGATATGAAGAAATTCCCNCANCAAGC
AACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAA
TAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATG
GATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGA
ATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGA
CTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCT
TGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGT
AAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAAC
CATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAA
TGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACC
ATCATCTCAAACTGATNATAATGTTGAATCTAAAAAAGATTCTTCTGCTAAACATGANTG
NTTATAAAT

---LKITXKXXXXVKKXXXSQKGNAVXCWVDMKKFPXQATHMGLSGKVVPAISVDSVANK
ARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDS
PKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTI
MLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDXNVESKKDSSAKHXXL
*

Frame A:
---hsr*xssxxxm*rkxxpxkkvmpfxvgsi*rnsxxkqliwvslvklfqpslliqlpi
klvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*t
hqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqp
scssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklximlnlkkilllnmxx
yk


Frame B:
---tqdnxqvxxxckenxxlxkr*crfxlgryeeipxxsnsygslw*scsshlc*fscq*
sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrl
tkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnh
halqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn*x*c*i*krffc*t*xx
in


Frame C:
---LKITXKXXXXVKKXXXSQKGNAVXCWVDMKKFPXQATHMGLSGKVVPAISVDSVANK
ARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDS
PKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTI
MLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDXNVESKKDSSAKHXXL
*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK467 (AFK467Q) /CSM/AF/AFK4-C/AFK467Q.Seq.d/                       1336   0.0
AHO764 (AHO764Q) /CSM/AH/AHO7-C/AHO764Q.Seq.d/                       1314   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1298   0.0
AFO829 (AFO829Q) /CSM/AF/AFO8-B/AFO829Q.Seq.d/                       1292   0.0
AFN252 (AFN252Q) /CSM/AF/AFN2-C/AFN252Q.Seq.d/                       1292   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1292   0.0
AFM402 (AFM402Q) /CSM/AF/AFM4-A/AFM402Q.Seq.d/                       1292   0.0
AFL789 (AFL789Q) /CSM/AF/AFL7-D/AFL789Q.Seq.d/                       1292   0.0
AFM835 (AFM835Q) /CSM/AF/AFM8-B/AFM835Q.Seq.d/                       1289   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1287   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   2e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   165   1e-39
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   163   5e-39
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   162   1e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   4e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   154   3e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   5e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   5e-35

 psg: 0.60  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFK467 is cyt

>AFK467Z.Seq
----------CCACTCAAGATAACANTCAAGTTNTNGNAANATGTAAAGAAAATNGNTNC
CTCNCAAAAAGGTAATGCCGTTTTNTGTTGGGTCGATATGAAGAAATTCCCNCANCAAGC
AACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAA
TAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATG
GATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGA
ATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGA
CTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCT
TGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGT
AAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAAC
CATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAA
TGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACC
ATCATCTCAAACTGATNATAATGTTGAATCTAAAAAAGATTCTTCTGCTAAACATGANTG
NTTATAAAT