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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK463
Library AF
(Link to library)
Clone ID AFK463
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16377-1
Original site URL
Representative seq. ID AFK463P
(Link to Original site)
Representative DNA sequence 
>AFK463 (AFK463Q) /CSM/AF/AFK4-C/AFK463Q.Seq.d/
ACTGTTGGCCTACTGGTATTCATCACCCTACAATTTAATTACATACATATATATATATAA
ACAATAATAAAAATGGGTTTCCGTTTCTTAGATATTGTTAAACCATTTACCTCACTCGTT
CCAGAGGTTGGTCAACCAGATAGAAAAATTCCTTTCCGTGAAAAGGTTCTTTGGACAGCA
ATTTGTTTATTCATTTTCTTAGTTTGCAGTCAAATTCCACTTTATGGTATTAGATCAACT
GATTCATCAGATCCATTTTACTGGGCTAAAGTTATTATGGCCTCAAACAGAGGTACTTTA
ATGGAATTAGGTATTTCACCAATCGTTACATCAGGTATGGTTATGCAATTATTAGCAGGT
GCCAAATTAATTGAAATTGATCAATCTGTTAAAGCCGATAGAGATTTATTCTCTGCTGCC
CAAAAATTATTTGGTATGTTAATTTGTGTAGGTCAAGGTGTAGCCTATATTTGGAGTGGT
TCATATGGTGATCCAGCAGTTTTAGGATTTGGTAACTGCTTTTTAATTGTTTTACAATTA
TTCTTTGCAGGTATTATTGNTATGCTTTTGGATGAACTTTTACAAAAAGGATATGGTATT
GGATXXXXXXXXXXATAACATTTTGGTAAACTTATTTGGTGCTTGGAGAACCAGTGAATA
TTCACAACAAATGATTCCAGTCTCTGGTNTCACCTATTACATCAGCTCCCCAAATAATAT
GAGCGCTGTTTTGGCCGATCCATTCCATGCCCTCTTTTACATCACCTTTATGTTGACAAG
TTGTGCTCTNTTCTCCAAAGTTTGGATCGAAGTTAGTGGTTCCTCTGCTCGTGACGTTGC
TAAGCAACTCAAAGATCAACAAATGACCATGAAAGGTCATCGTNATACCTCTGTCATCAA
GGAACTCAATCGTTATATTCCAACCGCTGCCGCTTTTGGTGGTCTCTGTATTGGTGCTCT
CACTGTCGTCGCCGATTTCATGGGTGCCATCGGTTCTGGTACNGGTATTTTATTAGCTGT
CACTATCATCTATCAATACTTTGAAACCTTTGTTAAAGAACAACAAGAACCTTCTGGTGG
TATTGGTGGTCTTTTCTAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllvfitlqfnyihiyi*TIIKMGFRFLDIVKPFTSLVPEVGQPDRKIPFREKVLWTA
ICLFIFLVCSQIPLYGIRSTDSSDPFYWAKVIMASNRGTLMELGISPIVTSGMVMQLLAG
AKLIEIDQSVKADRDLFSAAQKLFGMLICVGQGVAYIWSGSYGDPAVLGFGNCFLIVLQL
FFAGIIXMLLDELLQKGYGIG---

---NILVNLFGAWRTSEYSQQMIPVSGXTYYISSPNNMSAVLADPFHALFYITFMLTSCA
LFSKVWIEVSGSSARDVAKQLKDQQMTMKGHRXTSVIKELNRYIPTAAAFGGLCIGALTV
VADFMGAIGSGTGILLAVTIIYQYFETFVKEQQEPSGGIGGLF*
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvfitlqfnyihiyi*TIIKMGFRFLDIVKPFTSLVPEVGQPDRKIPFREKVLWTA
ICLFIFLVCSQIPLYGIRSTDSSDPFYWAKVIMASNRGTLMELGISPIVTSGMVMQLLAG
AKLIEIDQSVKADRDLFSAAQKLFGMLICVGQGVAYIWSGSYGDPAVLGFGNCFLIVLQL
FFAGIIXMLLDELLQKGYGIG---

---itfw*tylvlgepvnihnk*fqslvspitsapqii*alfwpihsmpsftsplc*qvv
lxspkfgsklvvpllvtllsnskink*p*kviviplssrnsivifqplpllvvsvlvlsl
sspiswvpsvlvxvfy*lslssintlkpllknnknllvvlvvfsk

Frame B:
llaywysspynlityiyiykq**kwvsvs*illnhlphsfqrlvnqiekflsvkrffgqq
fvysfs*favkfhfmvldqlihqihftglkllwpqtevl*wn*vfhqslhqvwlcny*qv
pn*lklinllkpieiysllpknylvc*fv*vkv*pifgvvhmviqqf*dlvtaf*lfyny
slqvllxcfwmnfykkdmvld---

---*hfgkliwclenq*ifttndsslwxhllhqlpk*yercfgrsipcpllhhlyvdklc
sxlqsldrs*wflcs*rc*atqrstndherssxylchqgtqslysnrcrfwwslywcshc
rrrfhgchrfwyxyfischyhlsil*nlc*rttrtfwwywwsfl

Frame C:
cwptgihhpti*lhtyiyinnnkngfpflryc*tiyltrsrgwstr*knsfp*kgsldsn
lfihflslqsnstlwy*in*firsillg*syyglkqryfngiryftnryirygyaiisrc
qin*n*sic*sr*rfilccpkiiwyvnlcrsrcslylewfiw*sssfriw*llfncftii
lcryyxyafg*tftkriwyw---

---NILVNLFGAWRTSEYSQQMIPVSGXTYYISSPNNMSAVLADPFHALFYITFMLTSCA
LFSKVWIEVSGSSARDVAKQLKDQQMTMKGHRXTSVIKELNRYIPTAAAFGGLCIGALTV
VADFMGAIGSGTGILLAVTIIYQYFETFVKEQQEPSGGIGGLF*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK463 (AFK463Q) /CSM/AF/AFK4-C/AFK463Q.Seq.d/                       1974   0.0
VFK877 (VFK877Q) /CSM/VF/VFK8-D/VFK877Q.Seq.d/                       1066   0.0
VFI788 (VFI788Q) /CSM/VF/VFI7-D/VFI788Q.Seq.d/                       1066   0.0
VFH420 (VFH420Q) /CSM/VF/VFH4-A/VFH420Q.Seq.d/                       1066   0.0
VFF234 (VFF234Q) /CSM/VF/VFF2-B/VFF234Q.Seq.d/                       1066   0.0
SFE222 (SFE222Q) /CSM/SF/SFE2-A/SFE222Q.Seq.d/                       1066   0.0
AFO294 (AFO294Q) /CSM/AF/AFO2-D/AFO294Q.Seq.d/                       1066   0.0
AFN640 (AFN640Q) /CSM/AF/AFN6-B/AFN640Q.Seq.d/                       1066   0.0
AFN556 (AFN556Q) /CSM/AF/AFN5-C/AFN556Q.Seq.d/                       1066   0.0
AFN358 (AFN358Q) /CSM/AF/AFN3-C/AFN358Q.Seq.d/                       1066   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CO047995|AF392805_1 probable transport protein Sec61 alpha subunit - Aedes aegypti. Score = 134 bits (337), Expect = 3e-31, mRNA sequence.    64   2e-27  4
BW493297|BW493297.1 Ciona intestinalis cDNA, clone:cima036a18, 3'end, single read.    80   6e-23  2
BW402016|BW402016.1 Ciona intestinalis cDNA, clone:ciem838d02, 3'end, single read.    80   6e-23  2
AL668163|AL668163.1 Ciona intestinalis; larval mRNA; EST 5PRIM end of clone 030ZH11 of  library directional larval cDNA library from Ciona intestinalis  (Ciona).    78   2e-22  2
BW071004|BW071004.1 Ciona intestinalis cDNA, clone:rcicl094l21, 3' end, single read.    78   2e-22  2
BW178699|BW178699.1 Ciona intestinalis cDNA, clone:rciht006h10, 3' end, single read.    78   2e-22  2
BW008403|BW008403.1 Ciona intestinalis cDNA, clone:rcibd033d16, 3' end, single read.    78   2e-22  2
BW222401|BW222401.1 Ciona intestinalis cDNA, clone:cilv049p08, 5' end, single read.    78   2e-22  2
BW001601|BW001601.1 Ciona intestinalis cDNA, clone:rcibd014j09, 3' end, single read.    78   2e-22  2
BW302109|BW302109.1 Ciona intestinalis cDNA, clone:cinc024g16, 5' end, single read.    78   2e-22  2
dna update 2004. 7.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XK2) RecName: Full=Protein transport protein Sec61 subunit a...   353   6e-96
AY737549_1(AY737549|pid:none) Toxoptera citricida probable trans...   260   5e-68
AY064125_1(AY064125|pid:none) Aedes aegypti clone bR19Mc3 probab...   258   3e-67
AB062670_1(AB062670|pid:none) Drosophila melanogaster mRNA for D...   257   4e-67
AK017615_1(AK017615|pid:none) Mus musculus 8 days embryo whole b...   257   5e-67
BC026179_1(BC026179|pid:none) Homo sapiens Sec61 alpha 2 subunit...   257   5e-67
FJ425873_1(FJ425873|pid:none) Bombyx mori transport protein Sec6...   256   7e-67
AF392805_1(AF392805|pid:none) Aedes aegypti strain RED probable ...   256   9e-67
BT059399_1(BT059399|pid:none) Salmo salar clone ssal-rgf-537-290...   256   1e-66
(Q98SN9) RecName: Full=Protein transport protein Sec61 subunit a...   256   1e-66
protein update 2009. 4.24
PSORT 

 psg: 0.81  gvh: 0.68  alm: 0.19  top: 0.47  tms: 0.47  mit: 0.31  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: plasma membrane
	 32.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for AFK463 is pla
5' end seq. ID AFK463F
5' end seq. 
>AFK463F.Seq
ACTGTTGGCCTACTGGTATTCATCACCCTACAATTTAATTACATACATATATATATATAA
ACAATAATAAAAATGGGTTTCCGTTTCTTAGATATTGTTAAACCATTTACCTCACTCGTT
CCAGAGGTTGGTCAACCAGATAGAAAAATTCCTTTCCGTGAAAAGGTTCTTTGGACAGCA
ATTTGTTTATTCATTTTCTTAGTTTGCAGTCAAATTCCACTTTATGGTATTAGATCAACT
GATTCATCAGATCCATTTTACTGGGCTAAAGTTATTATGGCCTCAAACAGAGGTACTTTA
ATGGAATTAGGTATTTCACCAATCGTTACATCAGGTATGGTTATGCAATTATTAGCAGGT
GCCAAATTAATTGAAATTGATCAATCTGTTAAAGCCGATAGAGATTTATTCTCTGCTGCC
CAAAAATTATTTGGTATGTTAATTTGTGTAGGTCAAGGTGTAGCCTATATTTGGAGTGGT
TCATATGGTGATCCAGCAGTTTTAGGATTTGGTAACTGCTTTTTAATTGTTTTACAATTA
TTCTTTGCAGGTATTATTGNTATGCTTTTGGATGAACTTTTACAAAAAGGATATGGTATT
GGAT----------
Length of 5' end seq. 604
3' end seq. ID AFK463Z
3' end seq. 
>AFK463Z.Seq
----------ATAACATTTTGGTAAACTTATTTGGTGCTTGGAGAACCAGTGAATATTCA
CAACAAATGATTCCAGTCTCTGGTNTCACCTATTACATCAGCTCCCCAAATAATATGAGC
GCTGTTTTGGCCGATCCATTCCATGCCCTCTTTTACATCACCTTTATGTTGACAAGTTGT
GCTCTNTTCTCCAAAGTTTGGATCGAAGTTAGTGGTTCCTCTGCTCGTGACGTTGCTAAG
CAACTCAAAGATCAACAAATGACCATGAAAGGTCATCGTNATACCTCTGTCATCAAGGAA
CTCAATCGTTATATTCCAACCGCTGCCGCTTTTGGTGGTCTCTGTATTGGTGCTCTCACT
GTCGTCGCCGATTTCATGGGTGCCATCGGTTCTGGTACNGGTATTTTATTAGCTGTCACT
ATCATCTATCAATACTTTGAAACCTTTGTTAAAGAACAACAAGAACCTTCTGGTGGTATT
GGTGGTCTTTTCTAAA
Length of 3' end seq. 486
Connected seq. ID AFK463P
Connected seq. 
>AFK463P.Seq
ACTGTTGGCCTACTGGTATTCATCACCCTACAATTTAATTACATACATATATATATATAA
ACAATAATAAAAATGGGTTTCCGTTTCTTAGATATTGTTAAACCATTTACCTCACTCGTT
CCAGAGGTTGGTCAACCAGATAGAAAAATTCCTTTCCGTGAAAAGGTTCTTTGGACAGCA
ATTTGTTTATTCATTTTCTTAGTTTGCAGTCAAATTCCACTTTATGGTATTAGATCAACT
GATTCATCAGATCCATTTTACTGGGCTAAAGTTATTATGGCCTCAAACAGAGGTACTTTA
ATGGAATTAGGTATTTCACCAATCGTTACATCAGGTATGGTTATGCAATTATTAGCAGGT
GCCAAATTAATTGAAATTGATCAATCTGTTAAAGCCGATAGAGATTTATTCTCTGCTGCC
CAAAAATTATTTGGTATGTTAATTTGTGTAGGTCAAGGTGTAGCCTATATTTGGAGTGGT
TCATATGGTGATCCAGCAGTTTTAGGATTTGGTAACTGCTTTTTAATTGTTTTACAATTA
TTCTTTGCAGGTATTATTGNTATGCTTTTGGATGAACTTTTACAAAAAGGATATGGTATT
GGAT----------ATAACATTTTGGTAAACTTATTTGGTGCTTGGAGAACCAGTGAATA
TTCACAACAAATGATTCCAGTCTCTGGTNTCACCTATTACATCAGCTCCCCAAATAATAT
GAGCGCTGTTTTGGCCGATCCATTCCATGCCCTCTTTTACATCACCTTTATGTTGACAAG
TTGTGCTCTNTTCTCCAAAGTTTGGATCGAAGTTAGTGGTTCCTCTGCTCGTGACGTTGC
TAAGCAACTCAAAGATCAACAAATGACCATGAAAGGTCATCGTNATACCTCTGTCATCAA
GGAACTCAATCGTTATATTCCAACCGCTGCCGCTTTTGGTGGTCTCTGTATTGGTGCTCT
CACTGTCGTCGCCGATTTCATGGGTGCCATCGGTTCTGGTACNGGTATTTTATTAGCTGT
CACTATCATCTATCAATACTTTGAAACCTTTGTTAAAGAACAACAAGAACCTTCTGGTGG
TATTGGTGGTCTTTTCTAAA
Length of connected seq. 1090
Full length Seq ID -
Full length Seq. -
Length of full length seq. -